• The Plant Pathology Journal
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• 제16권5호
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• pp.290-293
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• 2000

The main grape producing regions in Korea were surveyed for the occurrence and relative incidence of crown gall disease on grapevine. The results of the survey showed that the disease tended to affect tetraploid cultivars, which produce a large and sweet fruit but are very weak to cold weather. Incidence of crown gall disease was high on the tetraploid cultivars, Kyoho (Gerbong), Daebong, and Black Olympia while it was low on the diploid cultivars, Cambell Early and Sheridan. The disease incidence was very high on Anseong, Cheonan, and Chincheon, where the major growing areas of tetraploid cultivars and grapevines were burried in the winter to protect from freeze injury, whereas it was low in Yungdong, Kimcheon, and Nonsan. Crown gall disease did not increased with grapevine age on both Cambell Early and Kyoho. It remained low in Cambell Early, but high for all ages on Kyoho, Galls were found on the crown region and mid part of the trunk, but more galls were on small branches and canes on Kyoho grapevines. More than one third of ZKyoho grapevines inspected had galls on multiple locations on grapevines. On Kyoho, 56.3% of the galls covered more than 50% of the crown gall disease affects severely on the cultivar.

• The Plant Pathology Journal
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• 제39권2호
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• pp.220-227
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• 2023

Rose crown gall caused by Agrobacterium tumefaciens is a major disease that damages the production of cutroses in Korea. The effective prevention methods for this disease include the use of resistant varieties. This study was conducted to evaluate the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease with nodal explants in vitro. Among 180 A. tumefaciens strains, pathogenic strain RC12 was selected as an inoculant strain. The strain RC12 was identified based on characteristics of some selective media, pathogenicity test, and polymerase chain reaction analysis. Forty rose cultivars formed tumors on explants inoculated with A. tumefaciens RC12. However, 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to A. tumefaciens RC12 without forming any tumors. Six cultivars with tumor formation rates of over 30% formed initial tumors within 23 days after inoculation. Six cultivars with low tumor formation rates of around 5% formed initial tumors after 28 days of inoculation. It was found that gall formation rate was highly correlated with the initial gall formation period. Thus, the relationship between the period of gall formation and the rate of gall formation could be useful for assessing resistance to crown gall disease. In vitro inoculation methods could be used to evaluate resistance of cut-rose cultivars to crown gall diseases.

• 식물병연구
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• 제10권4호
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• pp.227-230
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• 2004

Occurrence and epidemiology of crown gall caused by A. tumefaciens in persimmon tree was investigated. Persimmon tree was more sensitive to crown gall disease than peach and jujube tree. Over 80% persimmon trees were infected to crown gall in all investigated region with no relation to planted years. Yield of infected tree was severely decreased to 54%. Incidence of crown gall in nursery field of persimmon tree was also severe and the infection rate of persimmon seedlings collected from market was 34%. It was guessed that the primary inoculum of crown gall caused by A. tumefaciens is infected persimmon seedlings.

• The Korean Journal of Ecology
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• 제2권1호
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• pp.15-20
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• 1978

Pathological and anatomical studies on the cause of the crown gall like symptoms associated with the chestnut nurse grafts were undertaken. The crown gall bacterium, Agrobacterium tumefaciens, was isolated from the gall tissues of chestnut nurese grafts by using selective media developed by S초개소 et al. and kado and Heskett. Typical crown gall symptoms appeared on tomato, castor bean and geranium plants 10~21 days following inoculation with the bacterium isolated from the gall tissues of chestnut nurse grafts. Agrobacterium tumefaciens was reisolated from crown gall tissues of tomato, castor bean and geranium. Anatomical studies on the origin, growth and differentiation of the gall tissues of the chestnut nurse grafts confirmed that the gall tissues are of crown gall origin. Masses of Agrobacterium tumefaciens were observed from gall tissues of chestnut nurse grafts, so it could be confirmed that the crown gall symptoms prevalent on chestnut nurse grafts are caused by the crown gall bacterium, Agrobacterium tumefaciens.

• 농약과학회지
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• 제5권3호
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• pp.50-53
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• 2001

최근 우리나라의 여러 작물에서 뿌리혹병(근두암수병(根頭癌睡病), crown gall)의 발생이 증가하고 있음이 보고되고 있으며, 특히 중요한 화훼작물인 장미를 재배하는 시설하우스에서 뿌리혹병의 발생은 매우 심한 피해를 가져오고 있다. 그럼에도 불구하고 이 병에 대한 방제는 거의 이루어지지 않고 있는 실정이다. 본 연구에서는 전 세계적으로 매우 성공적으로 뿌리혹병의 생물농약으로 개발된 Agrobacterium radiobacter K84의 장미 뿌리혹병 방제효과를 검정하여 국내에서 장미 뿌리혹병의 방제에 그 사용가능성을 확인하고자 하였다. 장마의 지상부에 인위적인 상처를 주고 병원균을 접종하기 전에 그리고 접종 후 A. radiobacter K84 균주 현탁액의 살포는 뿌리흑병의 발병률과 혹의 크기를 크게 감소시켰다. K84 균주를 처리한 장미에서 뿌리혹병의 발병률은 $5.0{\sim}6.7%$로서 병원균을 단독 접종한 대조구에서의 발병률 85%에 비하여 크게 낮았다. K84 균주를 처리한 장미에서 형성된 혹의 크기와 생체중도 병원균만을 접종한 대조구 장미에 형성된 혹의 $4{\sim}5%$로서 K84 균주의 처리에 의해 크게 감소하였다. 장미의 뿌리에 인위적인 상처를 주고 병원균의 접종 전 및 후에 K84 현탁액에 침지 처리한 경우에도 혹의 크기, 생체중 그리고 병 발생률이 크게 감소하였다. 전체적으로 K84 균주 치리에 의한 지상부 혹의 생물적방제가는 $92{\sim}94%$이었고, 뿌리에서의 뿌리혹병 생물적방제가는 $51{\sim}59%$이었다. 이상의 결과는 A. radiobacter K84 균주의 처리가 매우 효과적으로 장미 뿌리혹병을 방제할 수 있음을 보여주고 있다.

• 식물병연구
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• 제12권2호
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• pp.75-80
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• 2006

2003 년부터 2005 년까지 장미 주요 재배단지를 중심으로 뿌리혹병 발생을 조사한 결과 전지역에서 발생이 확인되었으며, 주로 토양재배보다는 양액재배되는 장미에서 발생이 더 심하였다. 뿌리혹병에 감염된 장미는 주로 지제부에 발생이 많았으며, 뿌리에 발생하여 생육이 부진해지는 피해를 나타내었다. 저항성 품종 선발에서는 '리틀 실버' 등 10 품종만이 저항성 품종으로 검정되었을 뿐 대부분이 감수성이었다. 특히 '골든게이트', '리틀마블', '로사로제티' 등은 매우 감수성이 높았다.

• 한국유기농업학회지
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• 제23권3호
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• pp.497-507
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• 2015

내병성 포도 신품종을 육성하기 위해서는 병해 저항성인 유전자원을 선발하는 것이 요구된다. 본 연구에서는 머스카딘포도와 플로리다 교잡종포도를 대상으로 병원균을 상처접종하여 포도나무 줄기혹병에 대한 저항성을 검정하였다. 3종류의 Agrobacterium vitis 균주를 접종한 포도나무는 품종 간에는 혹의 형성에 차이를 나타내었지만, 병원균의 균주 간에는 차이가 나타나지 않았다. 머스카딘포도는 많은 품종이 감수성이었으며, 'Gold Isle'과 'Africa Queen'은 고도의 감수성이었고 'Welder'와 'Jumbo'는 저항성이었다. 플로리다 교잡종포도는 머스카딘포도에 비해 덜 민감하였으며 'Daytona'와 'Stover' 등은 감수성이고, 'Blanc du Bois'는 중도감수성이었다. 본 연구는 머스카딘포도를 대상으로 줄기혹병에 대한 품종저항성을 판별함으로써 아속간 교잡을 통한 줄기혹병 저항성 포도 신품종육성에 중요한 정보를 제공할 것이다.

• 식물병연구
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• 제14권2호
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• pp.90-94
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• 2008

한국 포도나무의 뿌리혹병에 의한 발생생태를 조사하기 위하여 뿌리혹병이 발병주율 및 포도나무 재배포장에서 병원균을 측정하였다. 노지와 하우스재배 '거봉'의 뿌리혹병 발병주율은 각각 $0.4{\sim}97.9%$ 및 $1.4{\sim}3.8%$, '캠벨얼리'의 발병주율은 각각 $1.2{\sim}2.1%$ 및 $0{\sim}1.8%$이었다. Agrobacterium 속의 밀도는 뿌리혹병이 발생한 포장의 토양이 뿌리혹병이 발생하지 않은 토양보다 많이 분리되었다. 플레이트상에서 콜로니 형태와 크기로 21개의 포도나무 토양 샘플로부터 Agrobacterium spp. 480균주를 선발하였다. 이들 중 포도나무에 접종하였을 때 13균주만이 뿌리혹을 형성하였다.

• The Plant Pathology Journal
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• 제19권5호
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• pp.235-238
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• 2003

The resistance to crown gall in grape rootstocks was evaluated by inoculating cuttings from 27 grape rootstock varieties with Agrobacterium vitis strain Cheonan 493. Thmors were formed in all varieties of grape rootstocks tested in this study. The symptoms were observed in the stems of all plants tested including '196-17' and '41B'. Based on the measurement of tumor weight on the stems of grape rootstocks, '779P' was found to be higbly susceptible to crown gall. While some varieties such as 'Gloire', '140R', '101-14M', '3309C', and '333EM' were found to be relatively resistance to crown gall. Varieties such as '99R', '1447P', 'Rupestris du lot', 'll0R', 'Freedom', and '41B' were also found to be susceptibl~ to crown gall. Other~ varieties including 'l103P', 'Teleki. 5C', '420A', 'Golia', and '5BB' were found to be moderately susceptible.

• 농업과학연구
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• 제47권3호
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• pp.693-705
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• 2020

Grapes (Vitis spp. L.) are the third most produced fruit in the world. Crown gall disease caused by Agrobacterium vitis forms galls in the stems of the grapevines and reduces the vitality of the fruit trees, resulting in reduced yields. This pathogen has occurred in vineyards worldwide and caused serious economic losses. It is a soil-borne disease, so Agrobacterium vitis can survive for several years in vineyards and is difficult to control. Additionally, since there is no effective chemical control method, the most effective control method is the breeding of resistant varieties. To make the resistant variety, marker-assisted selection (MAS) enables fast breeding with low cost. In this study, we applied a genome-wide association study (GWAS), by combining phenotyping and genotyping-by-sequencing (GBS), for the development of a single nucleotide polymorphism (SNP) marker set related to crown gall disease using 350 grapevine varieties. As a result of the GBS based genotyping analysis, about 58,635 SNPs were obtained. In addition, the phenotypic analysis showed 35.2% resistance, 73% moderate susceptibility and 16.4% highly susceptibility. Moreover, after confirmation, two genes (VvARF4 and VvATL6-like) were shown to be related to crown gall disease based on the results of GWAS analysis, using the phenotypic data, and GBS. High-resolution melting analysis (HRMA) was performed using the Luna^® Universal Probe with real-time PCR to distinguish the melting peaks of the resistant and susceptible varieties. Our data show that these SNP markers are expected to be helpful in evaluating resistance against grapevine crown gall disease and in breeding.