• Korean Journal of Microbiology
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• v.51 no.2
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• pp.115-125
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• 2015

A monoclonal antibody AP64 IgM binds to human acute nonlymphocytic leukemia (ANLL) cell line HL60 and also cross-reacts with the homologous antigen in a rat ANLL cell. This antibody mediated by complement, has leukemia a suppression effect. In this study, we generated a recombinant single-chain variable domain fragment (ScFv) which were derived from $V_H$ and $V_L$ cDNA of AP64 IgM-secreting hybridoma by RT-PCR. The two variable regions were joined with a single 15 amino acid linker $(G_4S)_3$. This recombinant ScFv was expressed as a single polypeptide chain from Escherichia coli BMH 71-18. The recombinant ScFv was purified by applying the periplasmic extract to $Ni^+$-NTA-agarose affinity column and detected with westernblot. The purified recombinant ScFv recognized a surface antigen (about 30 kDa) of HL60 cell line which is the same antigen detected by parental AP64 IgM. But the affinity of ScFv for a surface antigen of HL60 was lower than that of the parental AP64 IgM, which needs to be further improved. Overall, the recombinant ScFv specific to HL60 might be a useful bioreagent for either diagnostic or therapeutic purposes.

• Korean Journal of Cognitive Science
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• v.10 no.1
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• pp.25-43
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• 1999

The focus of this study is on the elements of structure design that facilitate u user interaction with applications within cyberspace Structure design entails decisions regarding the optimal classification and hierarchical organization of information into s successively higher units. i.e .. the grouping of highly related information in the form of nodes of a site and the subsequent connection of nodes that are inter-related. The decisions are based on the designer's subjective classification framework. which is not always compatible with that of the user. We propose that the ensuing cognitive dissonance can be reduced via the employment of multiple hierarchies and cross-referencing links. Multiple hierarchies represent a single information space in terms of a number of single hierarchies. each of which represent a different perspective Cross-referencing refers to the inter-connection between the constituent hierarchies by providing a link to the alternate hierarchy for information that is most likely to be categorized in diverse manners by users with differing perspectives. In this study we conducted two empirical studies to gauge the effectiveness of multiple hierarchies and Cross-referencing links in the domain of cyber shopping malls. In the first phase. an experiment was conducted to determine how subjects classified given products with respect to two different perspectives for categorization. Experimental cyber malls were developed based on the results from the first phase to test the effectiveness of multiple hierarchies and cross-referencing links. Results show that the ease of navigation was higher for cyber malls that had implemented cross-referencing links are of greater value when used in conjunction with single hierarchical designs rather than multiple hierarchies. Users satisfaction with and ease of navigation was higher for cyber malls that had not implemented multiple hierarchies. This paper concludes with discussion of these results and their implications for designers of cyber malls.

• 한국전산유체공학회:학술대회논문집
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• 2004.03a
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• pp.183-189
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• 2004

The flow and the heat transfer about the cross-flow fin-tube heat exchanger in an out-door unit of a heat pump system has been numerically Investigated. Using the general purpose analysis code, FLUENT, the Navier-Stokes equations and the energy equation are solved for the three dimensional computation domain that encompasses multiple rows of the fin-tube. The temperature on the fin and tube surface is assumed constant but compensated later through the fin efficiency when predicting the heat-transfer rate. The contact resistance is also taken into consideration. The flow and temperature fields for a wide range of inlet velocity and fin-tube arrangements are examined and the results are presented in the paper. The details of the flow are very well captured and the heat transfer rate for a range of inlet velocity is in excellent agreement with the measured data. The flow solution provides the effective permeability and the inertial resistance factor of the heat exchanger if the exchanger were to be approximated by the porous medium. This information is essential in carrying out the global flow field calculation which, in turn, provides the inlet velocity lot the microscopic temperature-field calculation of the heat exchanger unit.

• Journal of Korean Library and Information Science Society
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• v.36 no.4
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• pp.77-98
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• 2005

The purpose of this research is to propose a powerful alternative in designing knowledge portals using Topic Maps(TM). To demonstrate the power of TM In constructing knowledge portals. we designed a TM-based korean folk music(pansori) site, tested It with an existing pansoree site (pansoree.com ) employing diverse query patterns : simple, advanced, associative, and cross referential Queries. The results show that the TM-based site outperforms the pansoree.com in searching time and steps. The TM-based site also provide novice users who do not know pansori domain with easy access to Information that they need.

• Journal of the Korean Society for information Management
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• v.15 no.3
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• pp.7-26
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• 1998

The large scale documents of the data repository are utilized to the diverse applications. In the cross-language information retrieval, if the words of a query contain polymorphic meanings, the system needs multilingual corpus to exactly translate to the target words. We constructed the financial comparable corpus, called KFCM(Korean Financial Corpus corresponding to MLCC Corpus), comparing to the MLCC Polylingual Documents which consisted with the 6 European languages. It is independently constructed under the DTD of MLCC comparable corpus, and can be utilized to the cross-language information retrieval. In this paper, we discussed about the application and construction procedures of KFCM which is public domain data.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.12
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• pp.113-116
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• 1994

Estimation of the evoked potential using the iterated bispectrum and cross-correlation (IBC) has been tried for both simulation and real clinical data. Conventional time average (TA) method suffers from synchronization error when the latency time of the evoked potential is random, which results in poor SNR distortion in the estimation of EP waveform. Instead of EP signal average in time domain, bispectrum is used which is insensitive to time delay. The EP signal is recovered by the inverse transform of the Fourier amplitude and phase obtained from the bispectrum. The distribution of the latency time is calculated using cross-correlation between EP signal estimated by the bispectrum and the acquired signal. For the simulation. EEG noise was added to the known EP signal and the EP signal was estimated by both the conventional technique and bispectrum technique. The proposed bispectrum technique estimates EP signal more accurately than the conventional technique with respect to the maximum amplitude of a signal, full width at half maximum(FWHM). signal-to-noise-ratio, and the position of maximum peak. When applied to the real visual evoked potential(VEP) signal. bispectrum technique was able to estimate EP signal more distinctively. The distribution of the latency time may play an important role in medical diagonosis.

• Proceedings of the KIEE Conference
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• 2006.07d
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• pp.1803-1804
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• 2006

The purpose of this paper is to implement a Labview based TFDR Real Time system through the instruments of Pci eXtensions for Instrumentation(PXI). The proposed load impedance measurement algorithm was verified by experiments via the implemented real time system. The TFDR real time system consisted of the reference signal design, signal generation, signal acquisition, algorithm execution and results display parts. To implement real time system, all of the parts wore programmed by the Labview which is one of graphical programming languages. In the application software implemented by the Labview we were able to design a suitable reference signal according to the length and frequency attenuation characteristics of the target cable and controled the arbitrary waveform generator(ZT500PXI) of the signal generation part and the digital storage oscilloscope(ZT430PXI) of the signal acquisition part. By using the TFDR real time system with the terminal resistor on the target cable, we applied to the load impedance measurements. In the proposed load impedance algorithm a normalized time-frequency cross correlation function and a cross time-frequency distribution function was employed to calculate the reflection coefficient and phase difference between the input and the reflected signals.

• 한국지구물리탐사학회:학술대회논문집
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• 2005.05a
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• pp.165-180
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• 2005

Impact seismic wave test is a method for nondestructive testing of concrete structure using of stress wave which is propagated and reflected from internal flaws within concrete structure and external surface, In this study, we performed non-destructive testing using impact seismic wave test for safety diagnosis of civil engineering structures. For this, I've compared and analized the result in the way of reflective method mostly using on one-dimension such as tunnel lining, and penetration method using the way of cross hole and tomography.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.39 no.10
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• pp.27-37
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• 2002

In this paper, we present various combined field integral equation (CFIE) formulations for the analysis of electromagnetic scattering from arbitrarily shaped three dimensional homogeneous dielectric body in the frequency domain. For the CFIE case, we propose eight separate formulations with different combinations of testing functions that result in sixteen different formulations of CFIE by neglecting one of testing terms. One of the objectives of this paper is to illustrate that not all CFIE are valid methodologies in removing defects, which occur at a frequency corresponding to an internal resonance of the structure. Numerical results involving far scattered fields and radar cross section (RCS) are presented for a dielectric sphere to illustrate which formulation works and which do not.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.197-200
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• 2002

In this paper, roll damping coefficients for a non-conventional cross section, which is herein named as 'step' model, are investigated numerically and experimentally. Experiments are extensively carried out to estimate the roll damping coefficients. Numerical estimations are also made with the help of numerical codes. For convenience, the roll damping is divided into wave-making component and viscous component. The wave-making component is determined using a potential code and the viscous component using a viscous flow code, in which the fluid domain is taken as unbounded. In order to validate the present approach, a typical cross section with bilge is considered and our results are compared with published data. The comparison shows a good agreement qualitatively. For the step model, numerical results are compared well with experimental data besides some quantitative discrepancies at a certain range of frequency. It is thought that the discrepancy might be caused by the ignorance of the free surface in viscous computations. It is found in the case of the step model that not only the viscous component but also the wave component increases considerably compared to the section with bilge.