• Earthquakes and Structures
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• v.24 no.1
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• pp.53-64
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• 2023

Earth fissures with several kilometers will inevitably approach or cross the metro line, significantly threatening the safety of the underground structure in the earth fissure site. However, the influence of the earth fissure site's amplification effect on the metro station's dynamic response is still unclear. A representative earth fissure in Xi'an was taken as an example to establish a numerical model of a metro station in the earth fissure site. The dynamic response characteristics of the metro stations at different distances from the earth fissure under various seismic waves were calculated. The results show that the existence of the earth fissure significantly amplifies the dynamic response of the nearby underground structures. The responses of the axial force, shear force, bending moment, normal stress, horizontal displacement, inter-story drift, and relative slip of the metro station were all amplified within a specific influence range. The amplification effect increases with the seismic wave intensity. The amplification effect caused by the earth fissure has relatively weak impacts on the axial shear, shear force, bending movement, normal stress, and horizontal movement; slightly larger impacts on the inter-story drift and acceleration; and a significant impact on the relative slip. The influence ranges of the axial force and normal stress are approximately 20 m. The influence ranges of the acceleration and inter-story drift can reach 30 m. Therefore, the seismic fortification level of the underground structure in the earth fissure site needs to be improved.

• Journal of applied mathematics & informatics
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• v.41 no.5
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• pp.1071-1084
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• 2023

We consider inviscid, incompressible homogeneous shear flows of variable cross section known as extended Rayleigh problem. For this extended Rayleigh problem, we derived instability region which intersect with semi-circle instability region under some condition. Also we derived condition for stability, upper bound for amplification factor and growth rate of an unstable mode.

• Korean Journal of Veterinary Service
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• v.45 no.1
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• pp.19-30
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• 2022

In this study, a simple loop-mediated isothermal amplification (LAMP) combined with visual detection method (vLAMP) assay was developed for the rapid and specific detection of African swine fever virus (ASFV), overcoming the shortcomings of previously described LAMP assays that require additional detection steps or pose a cross-contamination risk. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubation for 40 min at 62℃. The assay specifically amplified ASFV DNA and no other viral nucleic acids. The limit of detection of the assay was <50 DNA copies/reaction, which was ten times more sensitive than conventional polymerase chain reaction (cPCR) and comparable to real-time PCR (qPCR). For clinical evaluation, the ASFV detection rate of vLAMP was higher than cPCR and comparable to OIE-recommended qPCR, showing 100% concordance, with a κ value (95% confidence interval) of 1 (1.00~1.00). Considering the advantages of high sensitivity and specificity, no possibility for cross-contamination, and being able to be used as low-cost equipment, the developed vLAMP assay will be a valuable tool for detecting ASFV from clinical samples, even in resource-limited laboratories.

• Journal of Mechanical Science and Technology
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• v.17 no.9
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• pp.1388-1396
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• 2003

The hydrodynamic instability of the three-dimensional boundary layer on a rotating disk introduces a periodic modulation of the mean flow in the form of stationary cross flow vortices. The instability labeled Type II by Faller occurs first at lower Reynolds number than that of well known Type I instability. Detailed numerical values of the amplification rates, neutral curves and other characteristics of the two instabilities have been calculated over a wide range of parameters. Presented are the neutral stability results concerning the two instability modes by solving the appropriate linear stability equations reformulated not only by considering whole convective terms but also by correcting some errors in the previous stability equations. The present stability results agree with the previously known ones within reasonable limit. Consequently, the flow is found to be always stable for a disturbance whose dimensionless wave number is greater than 0.75. Some spatial amplification contours have been computed for the stationary disturbance wave, whose azimuth angle $\varepsilon$= 11.29$^{\circ}$ to 15$^{\circ}$ and for the moving disturbance wave, whose azimuth angle $\varepsilon$ = 12.5$^{\circ}$ to 15$^{\circ}$. Also, some temporal amplification contours have been computed for the stationary disturbance wave, whose azimuth angle $\varepsilon$= 11.29$^{\circ}$ to 15$^{\circ}$ and for the moving disturbance wave, whose azimuth angle $\varepsilon$= 12$^{\circ}$ to 15$^{\circ}$. The flow instability was observed by using a white titanium tetrachloride gas over rotating disk system. When the numerical results are compared to the present experimental data, the numerical results agree quantitatively, indicating the existence of the selective frequency mechanism.

• The Plant Pathology Journal
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• v.36 no.5
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• pp.497-502
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• 2020

Barley yellow dwarf virus (BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42℃) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with other cereal-infecting viruses, and the method was 100 times more sensitive than conventional reverse transcription polymerase chain reaction. Furthermore, the assay was validated for the detection of BYDV in both field-collected oat leaves and viruliferous aphids. Thus, the RT-RPA assay developed in the present study represents a simple, rapid, sensitive, and reliable method for detecting BYDV in oats.

• Wind and Structures
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• v.15 no.3
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• pp.189-208
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• 2012

Lock-in and drag amplification phenomena are studied for a flexible cantilever using a simplified fluid-structure interaction approach. Instead of solving the 3D domain, a simplified setup is devised, in which 2D flow problems are solved on a number of planes parallel to the wind direction and transversal to the structure. On such planes, the incompressible Navier-Stokes equations are solved to estimate the fluid action at different positions of the line-like structure. The fluid flow on each plane is coupled with the structural deformation at the corresponding position, affecting the dynamic behaviour of the system. An Arbitrary Lagrangian-Eulerian (ALE) approach is used to take in account the deformation of the domain, and a fractional-step scheme is used to solve the fluid field. The stabilization of incompressibility and convection is achieved through orthogonal quasi-static subscales, an approach that is believed to provide a first step towards turbulence modelling. In order to model the structural problem, a special one-dimensional element for thin walled cross-section beam is implemented. The standard second-order Bossak method is used for the time integration of the structural problem.

• Proceedings of the KSME Conference
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• 2000.11b
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• pp.585-590
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• 2000

The hydrodynamic instability of the three-dimensional boundary layer on a rotating disk introduces a periodic modulation of the mean flow in the form of stationary cross flow vortices. Detailed numerical values of the growth rates, neutral curves and other characteristics of the two instabilities have been calculated over a wide range of parameters. Presented are the neutral stability results concerning the two instability modes by solving new linear stability equations reformulated not only by considering whole convective terms but by correcting some errors in the previous stability equations. The present stability results are agree with the previously known ones within reasonable limit. The flow is found to be always stable for a disturbance whose dimensionless wave number at Re=1200 is greater than 0.75. Also, the spatial amplification contours have been calculated for the moving disturbance wave, whose azimuth angle is between ${\varepsilon}=15^{\circ}$ and $12.5^{\circ}$.

• Korean Journal of Optics and Photonics
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• v.11 no.3
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• pp.171-178
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• 2000

We present a numerical model which enables to analyze $1.47mu$m amplification band characteristics for thulium doped fluoride fiber amplifiers. We focused on upconversion pumping scheme, thus many transitions affecting $1.47mu$m band amplification was considered simultaneously. Backward propagating waves and transverse mode were also considered in the model. The parameters for modeling were then acquired using published experimental data and related theories such as Judd-Ofelt theory for radiative transition, empirical energy gap law for non-radiative transition, and McCumber relations for cross-sections. The simulation showed well-matched results with experiment and internal dynamics. amics.

• Research in Plant Disease
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• v.27 no.2
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• pp.79-83
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• 2021

The aim of the present study was to develop a sensitive and specific detection method for the rapid detection of apple scar skin viroid (ASSVd) in apple leaves. The resulting reverse transcription recombinase polymerase amplification (RT-RPA) assay can be completed in 10 min at 42℃, is 10 times more sensitive than conventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple stem grooving virus, apple stem pitting virus, and apple chlorotic leaf spot virus. The reliability of the RT-RPA assay was assessed, and the findings suggested that it can be successfully utilized to detect ASSVd in field-collected samples. The RT-RPA assay developed in the present study provides a potentially valuable means for improving the detection of ASSVd in viroid-free certification programs, especially in resource-limited conditions.

• Parasites, Hosts and Diseases
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• v.60 no.3
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• pp.201-205
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• 2022

Babesia microti is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, B. microti cytochrome c oxidase subunit I (cox1) was selected as the target gene, multiple primers were designed, and optimized by a recombinase-aided amplification (RAA) assay. The optimal primers and probe were labeled with fluorescein. The sensitivity of fluorescent RAA (fRAA) was evaluated using gradient diluents of the cox1 recombinant plasmid and genomic DNA extracted from whole blood of B. microti infected mice. The specificity of fRAA was assessed by other transfusion transmitted parasites. The analytical sensitivity of the fRAA assay was 10 copies of recombinant plasmid per reaction and 10 fg/µl B. microti genomic DNA. No cross-reaction with any other blood-transmitted parasites was observed. Our results demonstrated that the fRAA assay would be rapid, sensitive, and specific for the detection of B. microti.