• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.6
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• pp.532-538
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• 1990

This experiment was carried out to establish the efficiency of crop breeding on comparison of combination methods of single, 3-way, and double crosses and combination order of 4 winter wheat which were different in origin, source, and plant types, On comparison of 4 crossing modes, there appeared the earliest heading and the highest grain yield in double cross, and decreased in 3-way. single crosses. and parents in order, There showed the significant mean squares of GCA and SCA in 4${\times}$4 diallel analysis for grain yield and yield components. Grumil and Bezostaya 1 exhibited highest GCA effect of grain yield which appeared the actual highest grain yield. There appeared the highest SCA-effect in F$_1$ (Eunpamil/Bezostaya 1) showing 4.22. Of the 3 double crosses there exhibited the highest grain yield in F$_1$ (Grumil/Eunpamil/ /Lanota/Bezostaya 1). Two single crosses for this double cross ---F$_1$ (Grumil/Eunpamil) and F$_1$ (Lancota/Bezostaya 1) --- do not revealed directly for this yield, but combined each other by chromosome switch as combination of F$_1$ (Grumil/Lancota), F$_1$(Grumil/Bezostaya 1), F$_1$(Eunpamil/Lancota) and F$_1$(Eunpamil/Bezostaya 1) which appeared the higher grain yields and SCA-effects. Of the six 3-way crosses. F$_1$ (Lancota/Bezostaya 1/ /Eunpamil) expressed the highest grain yield. Its combinations were F$_1$ (Lancota/Eunpamil) and F$_1$ (Bezostaya 1/Eunpamil) combined by chromosome switch, which its grain yield and SCA-effect were higher.

• The Korean Journal of Mycology
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• v.47 no.2
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• pp.173-179
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• 2019

Sawdust cultivation of Lentinula edodes has been increasing in Korea. Fourteen strains were used to develop the best varieties of L. edodes, and hybridization was carried out by monohybrid cross. The number of hybridized strains was 1,638 among 3,100 combinations. They were cultivated on sawdust medium, and fruiting bodies were formed in 364 strains. Among them, 65 strains were selected as superior candidate strains based on the shape and size of the fruiting bodies. Forty strains formed fruiting bodies without lamellae structure. The shape of the stipe was cylindrical (255 strains), thick to lower part (15 strains), and thick to upper part (94 strains). By the combinations of 2462 n1-10 and 3420 n1-10, 2462 n1, 2462 n2, 2462 n10, and 3420 n3 were selected as excellent monokaryotic strains. These strains were considered to be superior monokaryotic strains that could be used for hybrid breeding.

• Korean Journal of Plant Taxonomy
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• v.45 no.4
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• pp.362-368
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• 2015

Two new and one unrecorded hybrids of the genus Asplenium were newly found in Korea. They were proved to be hybrid based on intermediate morphology between putative parents. The first hybrid is $A.{\times}uiryeongse$ C.S. Lee & K. Lee (Aspleniaceae), nom. nov. (vernacular name: geo-mi-sa-cheol-go-sa-ri). This new hydrid is a cross between the Asian walking fern, A. ruprechtii and A. pekinens in Uiryeong-gil, Mt. Bukansan, Seoul, Korea. The other new one occurs in Mt. Seongsan in Yeoncheon-gun, Gyeonggi-do, Korea, $A.{\times}montanus$ C.S. Lee & K. Lee, nom. nov. (vernacular name: san-kko-ri-go-sa-ri) which might have experienced multiple hybridization events between A. ruprechtii ${\times}$ A. trichomanes and A. incisum following the hybrid between A. ruprechtii and A. trichomanes. In addition, one unrecorded hybrid, $A.{\times}kitazawae$ Kurata & Hutoh (vernacular name: geo-mi-dol-dam-go-sa-ri), reported first from Japan, is found in natural habitats in Do-dong, Daegu-si, and is a hybrid, between the Asian walking fern and A. sarelli. These hybrid taxa grow in sympatric regions with both putative parental species. The diagnostic characters for each taxon and evidence for their hybridization have been suggested. Descriptions, illustrations, and photographs of these hybrid taxa in their habitats are provided from Korea.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.184-190
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• 2013

It is important for genetic resources to collect and identify in native medium-leaf type zoysiagrasses species distributed in Korea. This study was conducted to investigate morphological variation and characteristics of native medium-leaf type zoysiagrasses from coastal, island and inland regions in Korea. Among them, 75 collected lines was confirmed to have various morphological variations, accessions were classified into 2 main based group coastal and inland regions by morphological characteristics and site environment. Group I included Z. sinica type, this group showed 3.7 mm in leaf width, 29 in number of seed per spikelet and 5.0 mm in seed length. Group II included Z. japonica type, this group showed 4.4 mm in leaf width, 42 in number of seed per spikelet and 3.5 mm in seed length. There is a need for additional research on growth characteristics and the molecular level for the introgressive hybridization between species which confirmed that cross-pollination is possible due to protogyny. The individuals showing variations should be preserved as valuable genetic resources for the expansion of variations in zoysiagrasses, and the results of this investigation on the genetic resources collected will be highly valuable in breeding high quality turfgrass.

• Korean Journal of Plant Taxonomy
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• v.53 no.2
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• pp.126-147
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• 2023

The taxonomic identity of Elaeagnus ×maritima and E. ×submacrophylla (Elaeagnaceae) in Korea is unclear, yet they are presumed to be hybrid taxa based on their morphology. To determine their hybrid origins, a morphological analysis (field surveys and specimen examinations) and a molecular analysis involving two nuclear ribosomal DNA (nrDNA) regions (internal transcribed spacer and 5S non-transcribed spacer) and one chloroplast DNA (cpDNA) region (matK) were conducted. The morphological analysis revealed that E. ×maritima showed certain morphological similarities to E. glabra, whereas E. ×submacrophylla showed certain morphological similarities to E. pungens. However, the molecular analysis indicated that E. ×maritima exhibited additive species-specific sites of E. glabra and E. macrophylla in the nrDNA regions. Notably, E. ×submacrophylla showed various aspects, with some individuals exhibiting additive species-specific sites of E. pungens and E. macrophylla in the nrDNA and E. macrophylla sequences in the cpDNA regions, some individuals exhibiting E. macrophylla sequences in the nrDNA and E. pungens sequences in the cpDNA regions, and some individuals displaying E. macrophylla sequences in both the nrDNA and cpDNA regions, despite an intermediate morphology between E. pungens and E. macrophylla. These results indicate that these two species are of hybrid origin and frequently cross between parental and hybrid individuals.

• Horticultural Science & Technology
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• v.30 no.1
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• pp.73-78
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• 2012

KC00256, KC00406, KC00462, KC00463, KC00820, and KC00821, the genetic resources that have previously been reported as moderately resistant to Phytophthora capsici, as well as the line KC01322, a new source of moderate resistance introduced from Laos, were tested against two strains (Pc003 and Pc005) of P. capsici. We also determined the nuclear restorer genotypes of these lines, in regards to their interaction with cytoplasmic male sterility, through crossing the resources with cytoplasmic male sterile Punggok-A (Srfrf) and determining the fertility of the $F_1$ hybrids. The studied lines exhibited a low level of resistance to both the strains of P. capsici compared to highly resistant CM334, but their response was fairly consistent for both P. capsici strains. KC00406, KC00462, KC00463, and KC01322 produced stable, male fertile $F_1$ plants indicating that they are restorers with genotype N(S)RfRf. KC00821 produced male sterile $F_1$ plants and was identified as a maintainer with genotype Nrfrf. The $F_1$ plants of the KC00820 cross, however, set a few male fertile flowers in the greenhouse at seedling stage, then became male sterile after being transplanted to the plastic greenhouse soil in May and remained so to the end of the growing season. Therefore, KC00820 is an unstable maintainer with genotype Nrfrf. The moderate resistance exhibited by these genetic resources may be integrated into breeding programs aimed at promoting higher levels resistance via recurrent selection or hybridization.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.9
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• pp.1229-1233
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• 2006

To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.19
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• pp.8441-8445
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• 2014

Background: Paired-like homeodomain transcription factor 2 (PITX2) is another new marker in breast carcinoma since hypermethylation at P2 promoter of this gene was noted to be associated with poor prognosis. We investigated the expression of PITX2 protein using immunohistochemistry in invasive ductal carcinoma and its association with the established growth receptors such as estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth receptor 2 (HER2). Methods: We conducted a cross sectional study using 100 samples of archived formalin-fixed paraffin embedded tissue blocks of invasive ductal carcinoma and stained them with immunohistochemistry for PITX2, ER, PR and HER2. All HER2 with scoring of 2+ were confirmed with chromogenic in-situ hybridization (CISH). Results: PITX2 protein was expressed in 53% of invasive ductal carcinoma and lack of PITX2 expression in 47%. Univariate analysis revealed a significant association between PITX2 expression with PR (p=0.001), ER (p=0.006), gland formation (p=0.044) and marginal association with molecular subtypes of breast carcinoma (p=0.051). Combined ER and PR expression with PITX2 was also significantly associated (p=0.003) especially in double positive cases. Multivariate analysis showed the most significant association between PITX2 and PR (RR 4.105, 95% CI 1.765-9.547, p=0.001). Conclusion: PITX2 is another potential prognostic marker in breast carcinoma adding significant information to established prognostic factors of ER and PR. The expression of PITX2 together with PR may carry a very good prognosis.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.298-311
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• 2015

Grape is one of the important fruit crops around the world, and exposed to disease and pests, and internal or environmental stresses in the vineyards. Breeding and cultivation of new varieties of high quality-grapes resistant to diseases and pests and tolerant to stresses are the most important steps in the grape production. However, conventional breeding has laborious and time-consuming procedures in maintaining and selecting seedlings in the fields. Development of molecular breeding technology through understanding of molecular mechanism of useful traits can be used as an alternative strategy to improve the efficiency of grape breeding program by cross hybridization in grape development programs. The completion of the grape genome sequencing project provided the way to discover the novel genes and to analyze their functions. Comparative genomics, transcriptomic analysis, and the genome-wide identification and analysis of useful genes as well as development of molecular marker for valuable traits could provide novel insights into fruit quality and the responses to diseases and stresses, and can be used as important information in molecular breeding programs for grape development.

• Economic and Environmental Geology
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• v.17 no.4
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• pp.299-314
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• 1984

The Yeongdo Island in Busan City is a remnant of the latest Cretaceous volcano, and consists geologically of andesites, rhyolite tuff, pelitic and psammitic hornfelses, lapilli rhyodacite tuff of the Yucheon Group, felsite and felsite porphyry of the Bulgugsa intrusives, and Holocene sediments in ascending order. The hornfelses are bound to the Taejongdae Formation. The stratigraphic position of the Formation is determined definitely into the Yucheon Group, thus the geologic age is approximately the same with the volcanic rocks of the Group. The sediments had been thermally metamorphosed to make pelitic and psammitic hornfelses of the albite epidote hornfels facies by the effects of active hydrothermal circulation, vaporization, and hybridization of andesitic solution, or of basification of acidic intrusives. Thus, on occasion, those hornfelses are not used to be distinguished from the andesitic rocks in the southeastern part of the Korean peninsula. The paleocurrent direction determined from several cross-beddings of the Taejongdae Formation is suggested to be from southwest to northeast. Orbicular rocks occur in hornfelsed rhyolite tuff, pelitic- and psammitic-hornfelses, and felsite porphyry at a lot of outcrops in the area of southwestern shoreline of the Yeongdo Island. Orbicules in rhyolite tuff and hornfels in the island might have originated from diffusion processes of metasomatic metamorphism carried out by hydrothermal solution rised from the intrusive adamellite which may be emplaced deeply under the Yeongdo volcanics. Those orbicules are due to metasomatic, secondary, and epigenetic origin. Proto-, multi-shelled, and multi-cored orbicules are described in the orbicular tuff. But multi-cored orbicules are not found in the orbicular fornfels. 250 tuff-orbicules numbered sporadically are in $20,000m^2$ area of the locality of orbicular tuff. About 60 hornfels-orbicules occurred sporadically are in $1,700m^2$ area of the locality of orbicular hornfels in the Taejongdae Formation. Orbicules in felsite porphyry might have originated by diffusion reaction between xenoliths and a quiescent zone in felsite porphyry magma. Those are of igneous, primary, and syngenetic origin.