• 제목/요약/키워드: cross hybridization

검색결과 101건 처리시간 0.022초

자가불화합성 Brassica campestris에 있어서 단일유전자좌가설에 의해 분리되지 않는 S-유전자 계통의 분석 (Analysis of Non-segregated S-allele Strain by Single-Locus Hypothesis in Self-incompatible Brassica campestris)

  • 노일섭
    • Journal of Plant Biology
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    • 제36권2호
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    • pp.127-132
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    • 1993
  • Self-incompatibility in Brassica campestris is controlled by multi-allele system in a single genetic locus, the S locus, and it is elucidated that S-glycoproteins are S gene products. In this experiments, we examined the genetic mode(pollen tube behavior and segregation of S-glycoprotein), characteristic of S-glycoproteins and DNA constitution within nuclear genome on S gene family that unexplained by single locus model, and investigated the segregation pattern of S-glycoproteins in bred F1 generation. By diallel cross among the 15 plants within one family the existence of three types of homozygotes and three types of heterozygotes were observed, and segregation of S-allele could not explained by single locus model. From the results of IEF-immunoblot analysis for non-segregated individual plant, the segregation pattern of S specific bands was corresponded with results of diallel cross except with one case(SaSa genotype). The molecular weight of 6 different S-genotype varied in near by 50 kD, and each genotype expressed with 2 or 3 bands. Specific bands in SaSa, SbSb, ScSc has almost similar molecular weight between them. Southern analysis of genomic DNA probed with S-glycoprotein cDNA for 6 different genotypes revealed that there are clear difference in polymorphism, multiple bands of hybridization, when restriction enzymes of EcoR I were used. It could be assumed that there are several sequences related to the S-glycoprotein structural genes within their nuclear genome. Therefore, we suggested the possibilities that S-allele system could be controlled by multi-locus, that dominance-recessive interactions could be explained by modifier gene or supressor gene based on the results of abnormal segregation of S-glycoprotein in bred F1. The F2 analyses are progressing in now.

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현대미술에 나타난 사진의 활용과 그 유형학적 분류 (Application and Its Typological Classification of Photographs Shown in Contemporary Art)

  • 이경률
    • 비교문화연구
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    • 제33권
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    • pp.193-239
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    • 2013
  • A photograph, which was applied to contemporary art, plays a very important role in the whole of the multiplied and diversified contemporary arts today. The application of photos, which were shown in contemporary art following the 1950s, can be classified into five types according to role and function of a photo in the process of developing a work. However, this classification is shown a little ambiguously. That is because photos of being utilized by artists are indicated very diversely depending on their artistic strategy and situation. As the first type, a few painters utilize a photo as mirror of reality in order to materialize a challenging and revolutionary idea with going against traditional picture in their pictorial practice. As the second classification, especially American painters utilize a photo as material component of forming their picture like photo-montage or collage. The combined application of this medium is first doing genre de-construction and hybridization of post-modernism in the 1980s while ultimately aiming at social criticism or political satire. The third type implies particularly a case that avant-garde artists utilize a photo as evidence of a work. In this case, a photo is employed as evidence of a work, which disappears in the temporal space essentially like body art, land art, and performance. Also, as the fourth type is a case of utilizing a photo for artistic concept of a work, not pictorial practice of picture, it is utilized often as important artistic strategy of conceptual artists. The final type of utilizing photo is a case of applying photo as formative tool on behalf of dye-stuffs or crayon in order to record a section of reality, which always continues regardless of event or meaning any more, in the traditional picture. In this case, a work is indicated as a photo of having a form of picture, namely, as Forme-tableau. The main subject is indicated there as a daily case of being repeated always the extremely common and revived theme.

황화 스트라이프 대륜계 호접란 신품종 '옐로우 크림' 육성 (Development of a Phalaenopsis (P. Blume) Cultivar, 'Yellow Cream' with Striped Yellow Flower)

  • 빈철구;김진기;김수경;노치웅
    • 화훼연구
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    • 제19권3호
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    • pp.177-180
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    • 2011
  • 경남농업기술원 화훼육종연구소에서 1997년부터 교배조합 육성을 위하여 국내의 재배농가와 종묘업체로부터 유전자원을 수집한 후, 2000년부터 2006년에 걸쳐 교배 및 계통선발육종을 하였다. 2006년부터 2008년까지 3회의 특성검정을 거쳐서 호접란 신품종 '옐로우 크림'을 개발하였다. '옐로우 크림'은 황색스트라이프 대륜계 꽃을 가진 품종으로 설판은 적색을 띤다. 꽃 크기가 10.3 cm 이상이 될 정도로 꽃이 큰 편이며 대비품종에 비해 화형은 둥글고 립이 붉으며 꽃잎의 모양이 반원형으로 아름다운 모양을 가지고 있다. 또한 꽃배열도 우수하며 꽃수도 많은 편이다. 엽색은 연한 녹색이며 생육속도도 빠르고 재배하기가 용이하다. 평균 꽃 수명 또한 55일 이상으로 길다. 소수의 품종만이 시장에 나와 있는 황색 스트라이프 계통의 신품종으로서 국내외 시장에서 기호도가 높을 것으로 판단된다.

중형 백색계 다화성 심비디움 'Honey Girl' 육성 (Cymbidium 'Honey Girl' with White Color and Medium Plant Size)

  • 김미선;조해룡;이혜경;임진희;최성렬;김영진
    • 화훼연구
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    • 제16권4호
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    • pp.291-294
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    • 2008
  • 'Honey Girl' 품종을 개발하기 위하여 1995년도에 적색 설판이 있는 백색계열의 소형 심비디움 'Yakoto'을 모본으로 하고 착화성이 우수한 백색계 중대형종 'Marian Simplicity"를 부본으로 하여 교배를 실시하였다. 1996년부터 1999년까지 실생계통 57개체를 양성하면서 엽형, 화색, 화형이 우수한 개체를 선발하여 2000~2004년에 1, 2차 특성검정과 품평회 결과 생육 및 개화특성이 우수한 원교 'F1-13' 계통을 최종 선발하였다. 이 품종은 생육이 강건하고 화색이 선명하고 화형이 둥글고 꽃 폭이 약 7.7cm 정도로 크며 화경의 늘어짐이 적고 초세가 강하고 개화시기는 11월 하순에서 1월경이었다.

생육이 강한 대형 백색계 심비디움 'White Princess' 육성 (A New Cultivar Cymbidium 'White Princess' with White Color and Vigorous Growth)

  • 김미선;조해룡;이혜경;임진희;최성렬;김영진
    • 화훼연구
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    • 제16권4호
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    • pp.295-298
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    • 2008
  • 'White Princess'(화이트프린세스) 품종은 원예연구소에서 2005년도에 육성한 심비디움 품종이다. 이 품종을 개발하기 위하여 1995년도에 화색이 연한 자주색 계열의 심비디움 수집 유전자원 Cym. '2113'을 모본으로 착화성이 우수하고 꽃송이가 큰 연분홍색의 'Lucky Rainbow Randevious'를 부본으로 교배를 실시하였다. 1996년부터 1999년까지 실생계통 90개체를 양성하면서 엽형, 화색 및 화형이 우수한 개체를 선발하여 2000~2004년에 1,2차 특성검정과 품평회 결과 생육 및 개화특성이 우수한 '원교 F1-14' 계통을 최종 선발하였다. 생육이 강건하고 화색이 선명하고 화형이 둥글고 꽃 폭이 약 8cm 정도로 크며 개화시기가 12월에서 1월경이었다.

Gold Shell Nanocluster Networks in Designing Four-Branch (1×4) Y-Shape Optical Power Splitters

  • Ahmadivand, Arash;Golmohammadi, Saeed
    • Journal of the Optical Society of Korea
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    • 제18권3호
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    • pp.274-282
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    • 2014
  • In this study, closely spaced Au nanoparticles which are arranged in nanocluster (heptamer) configurations have been employed to design efficient plasmonic subwavelength devices to function at the telecommunication spectrum (${\lambda}$~1550 nm). Utilizing two kinds of nanoparticles, the optical properties of heptamer clusters composed of Au rod and shell particles that are oriented in triphenylene molecular fashion have been investigated numerically, and the cross-sectional profiles of the scattering and absorption of the optical power have been calculated based on a finite-difference time-domain (FDTD) method. Plasmon hybridization theory has been utilized as a theoretical approach to characterize the features and properties of the adjacent and mutual heptamer clusters. Using these given nanostructures, we designed a complex four-branch ($1{\times}4$) Y-shape splitter that is able to work at the near infrared region (NIR). This splitter divides and transmits the magnetic plasmon mode along the mutual heptamers arrays. Besides, as an important and crucial parameter, we studied the impact of arm spacing (offset distance) on the guiding and dividing of the magnetic plasmon resonance propagation and by calculating the ratio of transported power in both nanorod and nanoshell-based structures. Finally, we have presented the optimal structure, that is the four-branch Y-splitter based on shell heptamers which yields the power ratio of 23.9% at each branch, 4.4 ${\mu}m$ decaying length, and 1450 nm offset distance. These results pave the way toward the use of nanoparticles clusters in molecular fashions in designing various efficient devices that are able to be efficient at NIR.

맥류의 종속간 교잡에 관한 연구 제1보 교배방법 및 교배촉진물질의 처리가 교잡종자 생산에 미치는 영향 (Studies on the Intergeneric Crosses in Triticeae I. Influence of Crossing Methods and Chemicals on the Hybridization Rate in Wheat-barley Crossings)

  • 김봉연;홍병희;조장환;제상율
    • 한국작물학회지
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    • 제27권2호
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    • pp.111-115
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    • 1982
  • 속간교잡을 실시함에 있어서 교배방법 및 교배촉진 물질로 알려진 EACA와 $GA_3$의 처리가 결실율 향상에 미치는 영향을 구명하고 교배시기에 있어서 뢰수분과 노화수분중 어떤 처리가 교잡종 생산에 유리한가를 검토하여 생장조절제인 EACA와 $GA_3$를 함께 처리한 구와 EACA만을 처리한 구를 두고 생장조절제의 효과도 아울러 구명코저 시험한 바 그 결과를 요약하면 다음과 같다. 1. 대맥$\times$소맥 속간교잡을 실시할 때는 노화수분의 결실율(4.5%)이 뢰수분보다 훨씬 높았다. 그러나 배형성율은 오히려 뢰수분쪽이 높았다. 2. $GA_3$ 처리 효과는 크게 인정되었으나 EACA 단독 효과는 인정되지 않았다. 3. 대맥$\times$소맥을 속간교잡하여 2개체의 형태적으로 소맥쪽을 더 닮은 식물체를 얻었으며 이는 생육이 진행될수록 더욱 소맥과 유사하였다. 이밖에도 시험관내에서 얼마간 자란 9개의 식물체도 얻을 수 있었다.

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Cloning and Characterization of Liver cDNAs That Are Differentially Expressed between Chicken Hybrids and Their Parents

  • Sun, Dong-Xiao;Wang, Dong;Yu, Ying;Zhang, Yuan
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권12호
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    • pp.1684-1690
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    • 2005
  • Using mRNA differential display technique, we investigated differential gene expression in hybrids relative to their parents in a diallel cross involving four chicken breeds in order to provide an insight into the molecular basis of heterosis in chicken. The results indicated that there was extensive differential gene expression between chicken F1 hybrids and their parents which was classified into four kinds of patterns as following: (1) bands only detected in hybrid F1; (2) bands only absent in hybrid F1; (3) bands only detected in parent P1 or P2; (4) bands absent in parent P1 or P2. Forty-two differentially expressed cDNAs were cloned and sequenced, and their expression patterns were confirmed by Reverse-Northern dot blot. Sequence analysis and database searches revealed that genes showed differential expression between hybrid and parents were regulatory and functional genes involved in metabolism, mRNA splicing, transcriptional regulation, cell cycles and protein modification. These results indicated that hybridization between two parents can cause changes in expression of a variety of genes. In conclusion, that the altered pattern of gene expression in hybrids may be responsible for heterosis in chickens.

A New Hybrid, Dark Pink Spotted Type Phalaenopsis 'Pink Marble'

  • Kim, Mi-Seon;Lee, Young-Ran;Rhee, Hye-Kyung;Park, Sang-Kun;Shin, Hak-Ki;Jung, Hyang-Young;Lim, Jin-Hee
    • 원예과학기술지
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    • 제29권5호
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    • pp.503-506
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    • 2011
  • A new hybrid, Phalaenopsis 'Pink Marble' was made by the National Institute of Horticultural and Herbal Science, Rural Development Administration, in 2005. This hybrid was selected from self-crossed progenies of P. '21-1' (collected number) in 1999. In 2001, one line was selected based on the aspects such as flower color, leaf shape, flower stalk, and vigorous growth. Trials were conducted from 2003 to 2005 for evaluation and selection of this cultivar. 'Pink Marble' had a medium flowering habit and a dark pink spot (RHS, RPN74B) on white petal and sepal when fully opened. The number of flowers on each peduncle was 7.5, and flower diameter was 52.3 cm. The general impression of petals and sepals is a plate shape. The thick sepal could extend the long flowering time. The average length of leaf and peduncle were 16.5 cm and 6.8 cm, respectively. It had a half-erect leaf form, and was a fast-growing cultivar. This hybrid is relatively easy to clone.

잡종 3배체 어류 기름종개 - 왕종개 잡종군의 종간 교잡실험 (Interspecific Hybridization between Triploid Hybrid Fish, Cobitis sinensis - longicorpus and Two Diploid Species from Korea)

  • 김익수;이은희
    • 한국어류학회지
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    • 제7권1호
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    • pp.71-78
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    • 1995
  • 낙동강 상류의 여러 수계에서 거의 암컷으로 구성된 Cobitis sinensis - longicorpus complex 2배체와 3배체 집단이 높은 빈도로 출현하였다. 그 가운데, 수컷의 C. sinensis (2n=48) 및 C. longicorpus (2n=50)와 암컷 3배체 C. sinensis - longicorpus (3n=73)를 실험실내에서 인공 교잡 실험을 하였다. 그 결과 각 조합에서 얻은 자손의 체측 반문에는 부계 형질이 발현되었고 그들의 염색체는 모두 부계의 haploid genome이 포함된 diploid form (2n=48,49)이었다. 이상의 결과로 볼때 3배체 암컷인 Cobitis sinensis - longicorpus complex는 제 1 감수분열에서 uneven genome을 배제하고 제 2감수분열에 의한 반수체 ovum을 형성하는 독특한 생식 양상을 갖는 것으로 사료된다.

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