• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2004년도 정기총회 및 제33차 춘계 학술대회
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• pp.198-201
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• 2004

To investigate hydrostatic pressure (HP) effect on myofibrillar protein (Mf) extracted from bovine Semitendinosus muscle, Ca- and Mg-ATPase activities to evaluate denaturation of myosin and actin, and soluble protein contents were observed. In Mf treated with 100 MPa for 5 min was not observed denaturation of myosin and actin. In Mf treated with 200 MPa for 5 min, denaturation of myosin and actin were observed but inactivation rate was low (0.0136 $min^{-1}$). Inactivation rate of myosin and actin was dramatically increased above 300 MPa treatment. However denaturation of myosin and actin was not that critical with duration time. By increasing pressure size, the amount of myosin and actin in soluble protein eluted in 20 mM potassium phosphate buffer (pH 7.0) containing 0.6 M NaCl were decreased. SDS-PAGE of soluble protein released from Mf suspension in 0.1 M NaCl buffer (pH 7.0) showed that low molecular weight proteins (15${\sim}$36 KDa) were released by HP treatment above 200 MPa. From the results, denaturation of myosin and actin, and release of light molecule proteins of Mf were observed by HP treatment over 200 MPa.

• 한국전산구조공학회논문집
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• 제12권3호
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• pp.353-362
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• 1999

본 연구에서는 콘크리트 중력식 댐의 임계균열길이가 계산되었으며 또한 복합균열의 균열선단에서 유효응력 확대계수의 변화량이 조사되었다. 작용하중으로는 댐 상부면에 작용하는 정수압과 댐의 균열면에 작용하는 수압 및 자중으로 구성된 정하중, 그리고 댐 주위에서 발파작업이 수행되는 경우에 고려될 수 있는 발파진동 및 동수압으로 구성된 동하중이 사용되었다. 균열이 발생한 위치와 방향 및 발파진동의 크기에 따라 임계균열길이가 계산되었으며, 또한 복합균열의 형태 및 균열선단 간의 이격거리에 따른 유효응력확대계수의 변화량이 검토되었다.

• 대한조선학회논문집
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• 제49권2호
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• pp.116-123
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• 2012

This study aims to analyze the strength of pressure hull of a small submarine. The pressure hull of a submarine has to withstand very large differential pressure between hydrostatic pressure in submarine operating depth and atmospheric pressure in inner space of a submarine. To do that, the pressure hull is generally ring-stiffened cylindrical shell under external pressure. In this situation, there are some foreseeable failure modes of the pressure hull such as shell yielding, axisymmetric shell buckling, asymmetric shell buckling, overall buckling and buckling of end closure. We calculated collapse pressures of these failure modes with approximation and empirical formulas. And, to analyze critical buckling pressure, we performed eigenvalue analysis with finite element method tools.

• Steel and Composite Structures
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• 제19권1호
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• pp.1-19
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• 2015

The buckling analysis is presented for non-homogeneous (NH) orthotropic truncated conical shells subjected to combined loading of axial compression and external pressure. The governing equations have been obtained for the non-homogeneous orthotropic truncated conical shell, the material properties of which vary continuously in the thickness direction. By applying Superposition and Galerkin methods to the governing equations, the expressions for critical loads (axial, lateral, hydrostatic and combined) of non-homogeneous orthotropic truncated conical shells with simply supported boundary conditions are obtained. The results are verified by comparing the obtained values with those in the existing literature. Finally, the effects of non-homogeneity, material orthotropy, cone semi-vertex angle and other geometrical parameters on the values of the critical combined load have been studied.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2007년도 International Meeting of the Microbiological Society of Korea
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• pp.83-85
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• 2007

Deep-sea bacteria are adapted to extreme environments, such as high pressures and cold temperatures. We have isolated many piezophiles which grow well even under high pressures from deep-sea sediment. Shewanella violacea DSS12 and Moritella japonica DSK1 have the ability to grow at up to 70 MPa, and those bacteria have unique mechanisms of gene expression in response to high pressure conditions. The combination of gene expression systems in piezophiles, like the high pressure-dependent promoters and GFP reporter gene, may reveal highly fluorescent cells when exposed to high hydrostatic pressure conditions. It is predicted that a novel bio-sensing system can be made to probe high pressure environments using living bacteria. First, gene transformation into our piezophiles, strains DSS12 and DSK1, were examined. Eschericha coli S17-1 was used for bacterial conjugation with those piezophiles. As a result, the broad host range vector, pKT231, and the shuttle vector, pTH10, were successfully introduced to DSS12 and DSK1, respectively. Next, The pressure regulated promoters from DSS12 and DSK1 were cloned into proper vectors and combined with GFP as a reporter gene downstream of each promoter. The transformants of DSK1 and DSS12 with the recombinant pTH10 and pKT231 plasmid, which has cadA and glnA promoters (each of them is a pressure regulated promoter from DSK1 and DSS12, respectively) and GFP, were grown under high pressure and gene expression of GFP promoted by 50 MPa pressure was confirmed. This is a critical point to create a pressure-sensing bacteria, as the "High Pressure Glow Cells", which will indicate the level of environmental pressure using fluorescence of GFP as a reporter gene.

• Coupled systems mechanics
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• 제2권2호
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• pp.147-157
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• 2013

We numerically investigate the electronic band structure of carbon nanotubes (CNTs) under radial corrugation. Hydrostatic pressure application to CNTs leads to a circumferential wave-like deformation of their initially circular cross-sections, called radial corrugations. Tight-binding calculation was performed to determine the band gap energy as a function of the amplitude of the radial corrugation. We found that the band gap increased with increasing radial corrugation amplitude; then, the gap started to decline at a critical amplitude and finally vanished. This non-monotonic gap variation indicated the metal-semiconductor-metal transition of CNTs with increasing corrugation amplitude. Our results provide a better insight into the structure-property relation of CNTs, thus advancing the CNT-based device development.

• Journal of Dairy Science and Biotechnology
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• 제25권2호
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• pp.29-32
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• 2007

An understanding functional properties and molecular interactions of milk proteins was critical to improve qualities of fermented dairy products including yogurts and cheeses. Extensive rearrangements of casein particles were important factors to enhance whey separation in yogurt gel network. The use of high hydrostatic pressure treated whey protein as an ingredient of low fat processed cheese food resulted in the production of low fat processed cheese food with acceptable firmness and enhanced meltabilities. Milk protein-based nano particles produced by self-association of proteins could be better nutrient delivery vehicle than micro particle since particle size reduction in nano particles could lead to increased residence time and surface area available in GI tract.

• 한국유가공학회:학술대회논문집
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• 한국유가공기술과힉회 2007년도 추계학술발표대회
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• pp.31-37
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• 2007

An understanding functional properties and molecular interactions of milk proteins was critical to improve qualities of fermented dairy products including yogurts and cheeses. Extensive rearrangements of casein particles were important factors to enhance whey separation in yogurt gel network. The use of high hydrostatic pressure treated whey protein as an ingredient of low fat processed cheese food resulted in the production of low fat processed cheese food with acceptable firmness and enhanced meltabilities. Milk protein-based nano particles produced by self-association of proteins could be better nutrient delivery vehicle than micro particle since particle size reduction in nano particles could lead to increased residence time and surface area available in GI tract.

• 한국해양공학회지
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• 제14권2호
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• pp.28-35
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• 2000

In this paper a complicated structural behavior in collision and its effect of energy transmission to the collision bulkhead was examined through a methodology of the numerical simulation to obtain a ideal bow construction and a location of collision bulkhead against heat on collision. At present the bow structure is normally designed in consideration of its specific structural arrangement and internal and external loads in these areas such as hydrostatic and dynamic pressure wave impact and bottom slamming in accordance with the Classification rules and the specific location of collision bulkhead by SOLAS requirement. By these studies the behavior of the bow collapse due to collision was synthetically evaluated for the different size of tankers and its operational speed limits and by the results of these simulation it provides the optimal design concept for the bow construction to prevent the subsequent plastic deformation onto or near to the collision bulkhead boundary and to determine the rational location of collision bulkhead.

• 한국항만학회지
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• 제14권2호
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• pp.219-231
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• 2000

In this paper a complicated structural behavior in collision and its effects of energy translation to the collision bulkhead was examined through a methodology of the numerical simulation to obtain a ideal bow construction and a location of collision bulkhead against head on collision. In the present the bow structure is normally designed in consideration of its specific structural arrangements and internal and external loads in these area such as hydrostatic and dynamic pressure, wave impact and bottom slamming in accordance with the Classification rules, and the specific location of collision bulkhead by SOLAS requirement. By these studies the behavior of the bow collapse due to collision was synthetically evaluated for the different size of tankers and its operational speed limits, and by the results of these simulation it provides the optimal design concept for the bow construction to prevent the subsequent plastic deformation onto or near to the collision bulkhead boundary and to determine the rational location of collision bulkhead.