• Asian Journal of Turfgrass Science
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• v.14 no.1
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• pp.263-272
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• 2000

A field study was carried out to find out the effective chemical for controlling algae without visual injury on creeping bentgrass golf greens. The results were as follows. 1. Chlorothalonil(75%), Metalaxy(7.5%)+Mancozeb(56%) and Mancozeb(75%) did not injure creeping bentgrass. Only chlorothalonil(75%) effectively controlled algae regardless of concentration among above mentioned 3 fungicides. 2. Mancozeb frequently used on golf courses showed about 50% effect on controling algae in this study. 3. Mancozeb(75%)+Copper hydroxide(73%) and Mancozeb(75%)+Streptomycin(100%) had good effects on algae control while injuring creeping bentgrass. 4. Copper hydroxide(73%) and copper sulfate basic(58%) $including\ulcorner$Cu$\lrcorner$showed effective algae control but caused injure creeping bentgrass. 5. Streptomycin has been scarcely used on golf greens but streptomycin(20%) 0.1g a.i/$\m^2$ had good control of algae and durability without injuring creeping bentgrass.

• Journal of Plant Biotechnology
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• v.36 no.4
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• pp.327-335
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• 2009

Creeping bentgrass (Agrostis stolonifera L.) is economically important as the principal turfgrass species for golf course greens and fairways in temperate climates around the world. As the utilization area of the turfgrass species increases recently, the demand for new and improved cultivars increases. Thus, substantial progress has been made in applying modern biotechnology to develop genetically engineered (i.e., biotech) creeping bentgrass with new traits that eluded the breeders. This review article addresses the advances made in developing biotech creeping bentgrass, which are categorized in the following topics: (i) genetic transformation of creeping bentgrass, (ii) development of various biotech creeping bentgrasses by genetic engineering, and (iii) progresses in the deregulation of herbicideresistant creeping bentgrass.

• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.1
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• pp.101-110
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• 1994

A field study was carried out to investigate the visual injury of zoysiagrass and creeping bentgrass by the application of various herbicides the result were as follows. 1. Trifluralin + benefin and dithiopyr did not injure creeping bentgrass and zoysiagrass. 2. Creeping bentgrass was safe while zoysiagrass was slightly injured within acceptable level with benefin. 3. Oryzalin caused injury both on creeping bentgrass and zoysiagrass. However, the injury of zoysigrass was within acceptable level while the injury of creeping bentgrass increased without acceptable level when applied at>5kg/ha. 4. Creeping bentgrass was tolerant to pendimethalin only when treated at<3.4kg/ha whereas zoysiagrass was tolerant regardless of rate. 5. Creeping bentgrass treated with fenoxaprop, oxadiazon, and bensulide were severely injured. However, turfgrasses treated with bensulide recovered rapidly when compared with fenoxaprop and oxadiazon. 6. Zoysiagrass treated with 2,4-D, dicambe, bentazon was safe when applied at mid summer.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.267-276
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• 2006

To investigate the physiological responses to winter freezing stress naturally occurring, the level of lipid peroxidation and enzymatic antioxidant responses were compared between zoysiagrass and creeping bentgrass during overwintering. Root mortality of creeping bentgrass was significantly higher than zoysiagrass at January. Root growth of creeping bentgrass was nearly parallel with temperature fluctuation, while zoysiagrass showed little changes in root growth until the end of April. Total nonstructural carbohydrate of zoysiauass was 10% higher than creeping bentgrass. Malondialdehyde(MDA) content in creeping bentgrass was 2-fold higher than that of zoysiagrass. The peroxidase(POD) activity of creeping bentgrass in January was 4.2 times higher, while superoxide(SOD) and catalase(CAT) activities lowered 22% and 67%, respectively, compared to zoysiagrass. These results suggest that zoysiagrass roots much properly operate cold tolerance mechanism and: are less susceptible to cold stress in comparison to creeping bentgrass.

• Asian Journal of Turfgrass Science
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• v.26 no.2
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• pp.135-139
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• 2012

Creeping bentgrass (Agrostis stolonifera) is one of the most popular turfgrasses for high-quality playing surface such as putting green on golf courses and athletic fields. Continues damage such as divot injury on creeping bentgrass is major issue to maintain golf course properly. Although plentiful researches to maximize divot resistance have been reported, minimal research has focused on relation between nitrogen (N) sources and divot resistance. The study was conducted to determine the effect of N source for turfgrass divot recovery and overall tee performance. Eleven fertilizer treatments as N sources were applied to creeping bentgrass 'Penncross'. Before the first application, divot injuries were simulated by removing a core of soil and turfgrass from established plots and backfilling with native soil. Data collection included turfgrass color and quality. N release speed did not influenced divot recovery. Frequency of urea application had no effects on divot recovery. Urea with split application had no difference with no treatment for divot recovery. Polyon product especially polyon mini (41-0-0) had the best performance for divot recovery and for maintaining better turfgrass quality. Overall, small particle size of slow-release N form would influence creeping bentgrasss to recover divot damage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.3
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• pp.167-170
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• 2013

This study examined the growth performance and field evaluation of the dual herbicide-resistant transgenic creeping bentgrass plants. The effect of glyphosate treatment on the herbicide resistance of the transgenic creeping bentgrass plants was determined, and the non-transgenic control plant withered at the concentration $11{\mu}g/mL$ or higher whereas the transgenic creeping bentgrass plants survived the treatment at the concentration of $3,000{\mu}g/mL$, and the increase of the plant length was repressed as the glyphosate treatment concentration was increased. At field evaluation, glufosinate-ammonium and glyphosate were simultaneously treated to investigate the weed control effect. The results showed that more than 90% of the weeds withered four week after herbicide treatment, while the transgenic creeping bentgrass plants continued to grow normally. Therefore, the dual herbicide-resistant creeping bentgrass plants may be able to greatly contribute to the efficiency of weed control and to the economic feasibility of mowing in places such as golf courses.

• Weed & Turfgrass Science
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• v.1 no.4
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• pp.64-68
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• 2012

Creeping bentgrass (Agrostis stolonifera L.) is one of the highest maintained turfgrass but often problematic especially for Kentucky bluegrass fairway. Mesotrione is one of selective herbicide that is firstly registered for corn (Zea mays L.) and provides preemergence and postemergence control of broadleaf and annual grassy weeds. Although mesotrione is effective to eradicate area contaminated by creeping bentgrass, protracted time is required to recover damaged area by rhizome extension of Kentucky bluegrass. Overseeding is typically used to fill bare or damaged areas using appropriate turf species to create a uniform turfgrass surface. The objectives of this study were to evaluate mesotrione and seeding rate effects to recover Kentucky bluegrass contaminated by creeping bentgrass. Six treatments consisted of three mesotrione rates and two Kentucky bluegrass seeding rates. The mesotrione rate were 0, 0.05 and 0.10 m $ml^{-2}$. Two seeding rate of to Kentucky bluegrass 'Midnight' were 15 and 30 g $m^{-2}$. Mesotrione application and Kentucky bluegrass overseeding at the same time is helpful to damage creeping bentgrass but not for establishment of Kentucky bluegrass to refill damaged area. To maximize mesotrione effects, temperature above $20^{\circ}C$ would be recommended based on this study.

• Journal of Ecology and Environment
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• v.38 no.4
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• pp.437-442
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• 2015

Studies to estimate seed longevity and dormancy of creeping bentgrass (Agrostis stolonifera L.) were conducted from 2000 to 2005 at Corvallis and Hermiston, Oregon. Seeds from three transgenic glyphosate resistant creeping bentgrass lines, 48-10, 48-13, and ASR368, and one non-transgenic glyphosate susceptible line, SR1020, were used. Creeping bentgrass seeds were buried at 3, 18 and 31 cm in 2000 and removed 6, 12, 18, 24, and 51 months later. Soil type and climatic conditions were different at the two locations. At Corvallis, the soil was a Malabon silty clay loam, and the winters wet and mild. The soil at Hermiston was an Adkins fine sandy loam, and winters drier and colder. Seeds of all creeping bentgrass lines deteriorated faster at Corvallis than at Hermiston. The estimated half-lives of creeping bentgrass lines buried at Corvallis were 8.4 to 20.2 months, while those buried at Hermiston were 8.4 to 37.7 months. At both sites, seeds of the glyphosate resistant lines, 48-10 and 48-13, deteriorated faster than the susceptible line, SR1020. However, seed deterioration in the resistant line, ASR368, was slower than all other creeping bentgrass lines. Based on the germination test, exhumed intact seeds at Corvallis were more dormant than those at Hermiston. If buried, it could be expected that viable creeping bentgrass seeds will persist more than 4 years after the seeds are introduced to a site, but environmental conditions can influence both seed longevity and dormancy.

• Rapid Communication in Photoscience
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• v.3 no.2
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• pp.28-31
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• 2014

Loss of chlorophyll is the visible symptom of leaf senescence and staygreen refers to the delayed leaf senescence in plants. The staygreen gene (SGR) in rice (Oryza sativa L.) has been identified as its mutation maintains greenness during leaf senescence, and encodes a chloroplast protein required for the initiation of chlorophyll breakdown in plants. In this study, we isolated a rice SGR-homologous gene in creeping bentgrass (Agrostis stolonifera L.), and transgenic creeping bentgrass plants were obtained by introducing pCAMBIA3301 vector harboring antisense SGR gene under control of the senescence-specific SAG12 promoter. Transgenic plants were selected by herbicide resistance assays and genomic integration of the transgenes was confirmed by PCR analysis. Subsequent analyses demonstrated the staygreen phenotype of the transgenic creeping bentgrass plants with decreased chlorophyll loss during leaf senescence. These results suggest that the antisense SGR expression in creeping bentgrass delays leaf senescence, which provides a way to develop genetically engineered turfgrass varieties with the commercially useful staygreen trait.

• Journal of Environmental Science International
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• v.31 no.4
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• pp.311-318
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• 2022

In this study, we analyzed the relationships between weather factors and photosystem II activity (F_v/F_m), as a measure of photochemical efficiency, in three cool-season turfgrasses commonly planted on golf courses in Jeju, South Korea: perennial ryegrass (Lolium perenne L.), Kentucky bluegrass (Poa pratensis L.), and creeping bentgrass (Agrostis palustris Huds.). In all three turfgrasses, F_v/F_m was higher during late summer than during early summer. However, in late summer, F_v/F_m was significantly lower in perennial ryegrass than in the other two species. In early summer, F_v/F_m in perennial ryegrass and Kentucky bluegrass was positively correlated with mean low temperature and extreme minimum temperature, whereas, in late summer, this parameter in Kentucky bluegrass and creeping bentgrass was positively correlated with relative humidity, and in creeping bentgrass was negatively correlated with mean high temperature, mean low temperature, and extreme maximum temperature. These results indicate that raising low temperatures is favorable for perennial ryegrass and Kentucky bluegrass in early summer, whereas, in late summer, the lowering of high temperatures proves to be beneficial for creeping bentgrass, and raising relative humidity is conducive to the growth of Kentucky bluegrass and creeping bentgrass. These findings will contribute to improving the selection and management of turfgrasses on golf courses and sports fields.