• Title/Summary/Keyword: creeping

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Antisense expression of a staygreen gene (SGR) delays leaf senescence in creeping bentgrass

  • Hwang, Ok-Jin;Han, Yun-Jeong;Paek, Nam-Chon;Kim, Jeong-Il
    • Rapid Communication in Photoscience
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    • v.3 no.2
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    • pp.28-31
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    • 2014
  • Loss of chlorophyll is the visible symptom of leaf senescence and staygreen refers to the delayed leaf senescence in plants. The staygreen gene (SGR) in rice (Oryza sativa L.) has been identified as its mutation maintains greenness during leaf senescence, and encodes a chloroplast protein required for the initiation of chlorophyll breakdown in plants. In this study, we isolated a rice SGR-homologous gene in creeping bentgrass (Agrostis stolonifera L.), and transgenic creeping bentgrass plants were obtained by introducing pCAMBIA3301 vector harboring antisense SGR gene under control of the senescence-specific SAG12 promoter. Transgenic plants were selected by herbicide resistance assays and genomic integration of the transgenes was confirmed by PCR analysis. Subsequent analyses demonstrated the staygreen phenotype of the transgenic creeping bentgrass plants with decreased chlorophyll loss during leaf senescence. These results suggest that the antisense SGR expression in creeping bentgrass delays leaf senescence, which provides a way to develop genetically engineered turfgrass varieties with the commercially useful staygreen trait.

The Relationships between Weather Factors and Photosystem II Activity in Three Cool-season Turfgrasses in Summer (한지형 잔디 3종의 하절기 광계II 활성과 기상요인과의 상관성)

  • Koh, Seok Chan
    • Journal of Environmental Science International
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    • v.31 no.4
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    • pp.311-318
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    • 2022
  • In this study, we analyzed the relationships between weather factors and photosystem II activity (Fv/Fm), as a measure of photochemical efficiency, in three cool-season turfgrasses commonly planted on golf courses in Jeju, South Korea: perennial ryegrass (Lolium perenne L.), Kentucky bluegrass (Poa pratensis L.), and creeping bentgrass (Agrostis palustris Huds.). In all three turfgrasses, Fv/Fm was higher during late summer than during early summer. However, in late summer, Fv/Fm was significantly lower in perennial ryegrass than in the other two species. In early summer, Fv/Fm in perennial ryegrass and Kentucky bluegrass was positively correlated with mean low temperature and extreme minimum temperature, whereas, in late summer, this parameter in Kentucky bluegrass and creeping bentgrass was positively correlated with relative humidity, and in creeping bentgrass was negatively correlated with mean high temperature, mean low temperature, and extreme maximum temperature. These results indicate that raising low temperatures is favorable for perennial ryegrass and Kentucky bluegrass in early summer, whereas, in late summer, the lowering of high temperatures proves to be beneficial for creeping bentgrass, and raising relative humidity is conducive to the growth of Kentucky bluegrass and creeping bentgrass. These findings will contribute to improving the selection and management of turfgrasses on golf courses and sports fields.

Influence of NaCl on Seed Germination of Cool-Season Turfgrass species (NaCl이 한지형잔디 종자발아에 미치는 영향)

  • Kang, Hoon;Lee, Chi-Won
    • Asian Journal of Turfgrass Science
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    • v.13 no.2
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    • pp.71-78
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    • 1999
  • The influence of increasing livel (0.0, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1.2, 1.6, and 2.0%) fo NaCl on the germination of red fescue (Festuca rubra) 'Sea Breeze', tall fesce(Festucaarundinacea) 'Pixie', creeping red fescue (Festuca rubra ssp. rubra) 'Cindy', annual rye-grass (Lolium multiflorum) 'Permer Ⅱ', perennial ryegrass (Lolium perenne) 'Pennant', fairway wheatgrass(Agropyron cristatum), creeping bentgrass (Agrostis palustris) 'Penncross', and kentucky bluegrass (Poa pratensis) 'Nuglade' was investigated. Red fescue 'Sea Breeze', tall fescue 'Pixie', and creeping red fescue 'Cindy' had greater than 90% seed germination at NaCl concentrations of 0.2% or lower, while showing similar seeding shoot and root lengths and TAA50 values as the control. Creeping red fescue 'Cindy'gave at 0.6% or higher NaCl. Perennial ryegrass 'Pennant' and annual ryegrass 'Permer Ⅱ' showed more than 95% seed germination when NaCl concentrations were 0.4% and 0.8%, respectively. Fairway wheatgrass, creeping bentgrass 'Penncross' and Kentucky bluegrass 'Nugade' had showing similar germination percent, shoot and root lengths and T50 values as the control at NaCl concentrations of 0.1% or lower. In general, germination percent and the lengthes of seedling roots and shoots of all species tested decreased as NaCl concentrations iscreased. The T50 values became greater as NaCl concentration increased. Seed fermination in red fescue 'Sea Breeze', tall fescue 'Pixie',perennial ryegrass 'Pennant', and annual ryegrass 'Permer Ⅱ' was compoetely inhibited at 2.0% NaCl. Creeping red fescue 'Cindy' and fairway wheatgrass gardly germinated at 1.6% MaCl. Creeping bentgrass 'Penncross' and Dentucky bluegrass 'Nuglade' showed a complete inhibition of germination at 1.2% and 0.6% NaCl, respectively.

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Proposed Survey Steps for Investigation of Land-Creeping Susceptibility Areas: A Focus on Geophysical Mapping of the Yongheung-dong, Pohang, Korea

  • Kim, Jeong-In;Lee, Sun-Joong;Kim, Kwan-Soo;Lee, Jae-Eun;Sa, Jin-Hyun;Kim, Ji-Soo
    • The Journal of Engineering Geology
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    • v.31 no.3
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    • pp.269-281
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    • 2021
  • Land creeping is the imperceptibly slow, steady, downward movement o f slope-forming soil or rock. Because creep-related failures occur frequently on a large scale without notice, they can be hazardous to both property and human life. Korea Forest Service has operated the prevention and response system from land creeping which has been on the rise since 2018. We categorized and proposed three survey steps (e.g., preliminary, regional, detailed) for investigation of creeping susceptibility site with a focus on geophysical mapping of a selected test site, Yongheung-dong, Pohang, Korea. The combination of geophysical (dipole-dipole electrical resistivity tomography and reciprocal seismic refraction technique, well-logging), geotechnical studies (standard penetrating test, laboratory tests), field mapping (tension cracks, uplift, fault), and comprehensive interpretation of their results provided the reliable information of the subsurface structures including the failure surface. To further investigate the subsurface structure including the sliding zone, we performed high-resolution geophysical mapping in addition to the regional survey. High-resolution seismic velocity structures are employed for stability analysis because they provided more simplified layers of weathering rock, soft rock, and hard rock. Curved slip plane of the land creeping is effectively delineated with a shape of downslope sliding and upward pushing at the apex of high resistive bedrock in high-resolution electrical resistivity model with clay-mineral contents taken into account. Proposed survey steps and comprehensive interpretation schemes of the results from geological, geophysical, and geotechnical data should be effective for data sets collected in a similar environment to land-creeping susceptibility area.

Growth and Abscisic Acid Changes of Creeping Thyme in the Exposure of NaCl and Drought (염 및 건조스트레스 하에서 포복형 백리향의 생육과 Abscisic Acid 농도변화)

  • Kim, Min-Jea;Eom, Seok-Hyun
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.5
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    • pp.328-334
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    • 2009
  • Experimental purpose was to evaluate growth characteristic and abscisic acid (ABA) responses against salt/drought stresses. In the shoot biomass, creeping thyme was tolerated in mild NaCl stress, ranging 0 to 100 mM, while it was severely reduced in higher salinity. Under constant drought stress, the shoot biomass of creeping thyme showed a worse value compared to that of 100 mM NaCl treatment. Chlorophyll degradation was more severe in immature leaf than mature leaf under salt and drought stresses. In salt stress, immature leaf produced much amounts of ABA compared to mature leaf and also immature leaf showed faster increase of ABA than that of mature leaf. In drought stress, immature leaf responded to stress within 24 hours by the increase of ABA, while mature leaf responded to at 72 hours. Our results recommended that the optimal salinity level of creeping thyme was 50~100 mM NaCl.

Air Damping Evaluation for Laterally Driven Electrostatic Repulsive-Force Microactuators Using Creeping Flow Model (수평 구동형 정전 반발력 마이크로엑추에이터의 Creeping 유동 모델에 의한 공기 감쇠)

  • Lee, Gi-Bang;Seo, Yeong-Ho;Jo, Yeong-Ho
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.50 no.11
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    • pp.581-588
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    • 2001
  • This paper presents theoretical and experimental study on the quality-factor of the laterally oscillated electrostatic microactuator, driven by a lateral repulsive-force generated by the asymmetry of planar electric field. The quality-factor of the repulsive-force microactuator using a creeping flow model of the ambient air is evaluated. By filling the simulation results of damping force, we evaluate the dimensionless damping force, $\alpha$, thereby obtaining an analytical damping force, F, in the form of $F=\mu\; \alphaUL,\; where\; \mu,$ U and L denote the air viscosity, the velocity and the characteristic length of the movable electrodes. The measured quality-factor increases from 12 to 13 for the DC bias voltage increased from 60V to 140v. The theoretical quality-factor estimated from the creeping flow model increases from 14.9 to 18.7. Characteristics of quality-factor of the repulsive-force microactuator have been discussed and compared with those of the conventional attractive-force microactuator.

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Identification of Microdochium bolleyi Associated with Basal Rot of Creeping Bent Grass in Korea

  • Hong, Sung-Kee;Kim, Wan-Gyu;Choi, Hyo-Weon;Lee, Sang-Yeob
    • Mycobiology
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    • v.36 no.2
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    • pp.77-80
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    • 2008
  • Symptoms of basal rot occurred sporadically on creeping bent grasses growing at a golf course in Hampyeong, Korea in April 2007. Ten isolates of Microdochium sp. were obtained from leaves and crowns of the diseased bent grasses. All isolates were identified as Microdochium bolleyi based on morphological, cultural, and molecular characteristics. This is the first report on M. bolleyi associated with basal rot on creeping bent grass in Korea.

Characteristics and Factors Affecting Algae Development on Creeping Bentgrass Greens (크리핑 벤트그래스 그린에 발생되는 조류(Algae)의 특성과 발생원인)

  • 이혜원;정대영;심상렬
    • Asian Journal of Turfgrass Science
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    • v.14 no.1
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    • pp.257-262
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    • 2000
  • This study was conducted on ten golf courses in the vicinity of Yongin city to find out the kinds of algae and factors affecting algae development on creeping bentgrass greens. 1. Serious algae contamination was observed on almost of creeping bentgrass greens after and before rainy season. 2. There were ten kinds of algae occurred on creeping bentgrass greens, most of which were Oscillatoria spp. (bluegreen algae) 3. The fact that the same kind of algae inhabiting in the pond was observed on greens indicated that greens were easily contaminated by algae in case of greens watered with the pond. 4. This study showed that the main factor affecting algae contamination on greens was not high total nitrogen and phosphorus contents in the green soil, but pond watering.

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Control Effects of Microbial Products on Pythium Blight, Brown Patch and Dollar Spot of Creeping Bentgrass (Creeping Bentgrass에서 미생물제에 의한 Pythium Blight, Brown Patch 및 Dollar Spot 방제 효과)

  • 황연성;최준수;김영호
    • Korean Journal Plant Pathology
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    • v.12 no.2
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    • pp.237-244
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    • 1996
  • 길항곰팡이 3종(Aspergillus sp. A101, Penicillium sp. B202, Trichoderma sp. C303), 길항세균 3종(Arthrobacter sp. AN303)또는 이들 모두의 혼합균주를 배양하여 creeping bentgrass 양묘장에 처리하여 Pythium blight, brown patch 및 dollar spot의 방제효과를 조사하였다. 또한 길항미생물 6종의 배양액을 살균제와 병용하여 그린에 살포하여 이들 토양병에 대한 방제효과를 조사하였다. 양묘장에서는 미생물의 종류에 관계없이 Pythium blight 억제효과가 커 3회 이상 미생물제 처리시 병이 전혀 발생하지 않았다. 그러나 brown patch와 dollar spot은 미생물제에 의한 방제효과가 크지 않았으며, 살균제에 의해 효과적으로 방제되었다. 미생물제와 살균(Pythium blight 방제용 살균제 제외)를 병용하여 처리한 그린에서는 살균제 단독처리와 비교할 때 brown patch는 유의적으로 억제되었고 Pythium blight와 dollar spot은 차이가 없었다.

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Influence of Cultivated Conditions and Fungicides on Development of Rhizoctonia Diseases of Zoysiagrass and Bentgrass (한국들잔디와 벤트그라스의 라이족토니아 마름병 발생에 미치는 재배조건 및 살균제의 영향)

  • 이재홍;이두형
    • Asian Journal of Turfgrass Science
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    • v.9 no.3
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    • pp.199-206
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    • 1995
  • This study is aimed to clarify on the influence of cultivated conditions and fungicides on development of Rhizoetonia diseases of zoysiagrass and creeping bentgrass and results are as follows. Infection rates of disease were observed more serious when inoculum amounts were higher than low. Effects of the watering intervals on the severity of disease with artificial inoculating conditions, infection rates were higher when two days watering interval for creeping bentgrass and one day interval for zoysiagrass. Disease severity of creeping bentgrass incited by Rhizoetonia solani AG 1 (1A) was grater under conditions of double amounts of nitrogen fertilization. A posi- tive correlation was founded between the pH and disease development. The pots with pH 5 and 8 were significantly more diseased grass than those in which the pH was in 6.5. Mepronil and myclobutanil were extremely effective against brown patch of creeping bentgrass and large patch of zoysiagrass and followed by capro, and tebeonazole. Fenari was inferior effect to both diseases.

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