• Journal of the Korean Vacuum Society
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• v.18 no.3
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• pp.176-185
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• 2009

The realization and the performance of ICP source are strongly affected by its electrical impedance and the electric/magnetic field distribution. The ICP source impedance is determined by the antenna impedance and the plasma one. It is preferred to keep the imaginary impedance between -100 ohm to 100 ohm, since it should be avoided the high voltage formation on the antenna and abrupt impedance variation during the thin film process. The plasma uniformity is affected by the electric and magnetic field which is formed by the antenna current and voltage. The influence of azimuthal symmetry are shown by the electromagnetic simulation and the measurement result of plasma density. The radial uniformity can be controlled by locating the concentric antennas which have different diameters. The power distribution ratio and its control method are presented in the case of parallel antenna connections.

• Journal of Korea Water Resources Association
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• v.45 no.11
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• pp.1187-1199
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• 2012

A hybrid forecasting scheme based on wavelet decomposition coupled to a support vector machine model is presented for water demand series that exhibit nonlinear behavior. The use of wavelet transform followed by the SVM model of each leading component is explored as a model for water demand data. The proposed forecasting model yields better results than a traditional ARIMA time series forecasting model in terms of self-prediction problem as well as reproducing the properties of the observed water demand data by making use of the advantages of wavelet transform and SVM model. The proposed model can be used to substantially and significantly improve the water demand forecasting and utilized in a real operation.

• Journal of the Korean Vacuum Society
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• v.16 no.2
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• pp.110-115
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• 2007

A plasma enhanced atomic layer deposition(PEALD) system has been constructed adopting an inductively coupled plasma(ICP) source with an ALD system, and its plasma generation was carried out. Cobalt thin films were deposited on a p-type Si(100) wafer at $230^{\circ}C$. $Co_{2}(CO)_{6}$ was used as a cobalt precursor, $NH_{3}$ as a reactant, and Ar as a carrier and purge gas. The properties of the thin films were investigated using field emission scanning electron microscopy(FESEM) and auger electron spectroscopy(AES). Large amounts of impurities were found in both the ALD film and the PEALD film, however, the amount of impurities in the PEALD film was reduced to about 50 % compared to that in the ALD film. It was found that $NH_{3}$ plasma, very effectively, induces the reaction with carbon in a cobalt precursor.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.8
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• pp.693-700
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• 2008

The primary mirror system in a satellite camera is an opto-mechanically coupled system for a reason that optical and mechanical behaviors are intricately interactive. In order to enhance the opto-mechanical performance of the primary mirror system, opto-mechanical behaviors should be thoroughly investigated by using various analysis procedures such as elastic, thermo-elastic, optical and eigenvalue analysis. In this paper, optimal design of the bipod flexure mounts for high opto-mechanical performance is performed. Optomechanical performances considered in this paper are RMS wavefront error under the gravity and thermal loading conditions and 1st natural frequency of the mirror system. The procedures of the flexure mounts design based on design of experiments and statistics is as follows. The experiments for opto-mechanical analysis are constructed based on the tables of orthogonal arrays and analysis of each experiment is carried out. In order to deal with the multiple opto-mechanical properties, MADM (Multiple-attribute decision making) is employed. From the analysis results, the critical design variables of the flexure mounts which have dominant influences on opto-mechanical performance are determined through analysis of variance and F-test. The regression model in terms of the critical design variables is constructed based on the response surfaceanalysis. Then the critical design variables are optimized from the regression model by using SQP algorithm. Opto-mechanical performance of the optimal bipod flexure mounts is verified through analysis.

• Journal of KIISE:Computing Practices and Letters
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• v.10 no.5
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• pp.394-405
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• 2004

To make Web Services the real applications, the efficient mechanisms for Web Services discovery, Web Services composition and Web Services execution must be provided. Among these issues, especially, Web Services composition plays the key roles in Web Services applications that are loosely coupled and composed applications consisted of primitive Web Service components. In this paper we demonstrate a new Web Service composition approach using ontologies. We apply ontologies to describe Web Services information such as Web Services input/output parameters, pre conditions, post conditions and other necessary management information. In this paper, we also introduce Action ontology and Object ontology to describe the functional properties of Web Services These ontologies offer semantic description of Web Services functionalities beyond the limitation of the current WSDL. We can achieve semantic interoperabilities between heterogeneous Web Services in terms of conceptual processing and realize semantic services composition. We implement semantic Web Services composition system called Genie based on service description ontologies.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.536-545
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• 2019

Cytochrome P450 (P450 or CYP) is involved in the metabolism of endogenous and exogenous compounds in most organisms. P450s have great potential as biocatalysts in the pharmaceutical and fine chemical industries because they catalyze diverse oxidative reactions using a wide range of substrates. The high-cost nicotinamide cofactor, NADPH, is essential for P450 reactions. Glucose-6-phosphate dehydrogenase (G6PDH) has been commonly used in NADPH-generating systems (NGSs) to provide NADPH for P450 reactions. Currently, only two G6PDHs from Leuconostoc mesenteroides and Saccharomyces cerevisiae can be obtained commercially. To supply high-cost G6PDH cost-effectively, we cloned the cytosolic G6PDH gene of Solanum lycopersicum (tomato) with 6xHis tag, expressed it in Escherichia coli, and purified the recombinant G6PDH (His-G6PDH) using affinity chromatography. In addition, enzymatic properties of His-G6PDH were investigated, and the His-G6PDH-coupled NGS was optimized for P450 reactions. His-G6PDH supported CYP102A1-catalyzed hydroxylation of omeprazole and testosterone by NADPH generation. This result suggests that tomato His-G6PDH could be a cost-effective enzyme source for NGSs for P450-catalyzed reactions as well as other NADPH-requiring reactions.

• Coupled systems mechanics
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• v.7 no.3
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• pp.297-319
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• 2018

In this paper, the vibro-acoustic behaviors of vibrational cylindrical shells are investigated by using structural intensity approach. The reducing interior noise method for vibrating cylindrical shells is proposed by altering and redistributing the structural intensity through changing the damping property of the structure. The concept of proposed novel method is based on the properties of structural intensity distribution on cylindrical shells under different load and damping conditions, which can reflects power flow in the structures. In the study, the modal formulas of structural intensity are developed for the steady state vibration of cylindrical shell structures. The detailed formulas of structural intensity are derived by substituting modal quantities, in which the effect of main parameters such as weight coefficients and distribution functions on structure intensity are analyzed and discussed. Numerical simulations are first carried out based on the structural intensity analytical solutions of modal formulas. Through simulating the coupling vibration and acoustical radiation problems of cylindrical shell, the relationship between vibro-acoustic and structural intensity distribution is derived. We find that for cylindrical shell, by properly arranging damping conditions, the structural intensity can be efficiently changed and further the noise property can be improved. The proposed methodology has important implications and potential applications in the vibration and noise control of fuselage structure.

• Steel and Composite Structures
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• v.26 no.4
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• pp.473-484
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• 2018

Free vibration of cross-ply laminated plates using a higher-order shear deformation theory is studied. The arbitrary number of layers is oriented in symmetric and anti-symmetric manners. The plate kinematics are based on higher-order shear deformation theory (HSDT) and the vibrational behaviour of multi-layered plates are analysed under simply supported boundary conditions. The differential equations are obtained in terms of displacement and rotational functions by substituting the stress-strain relations and strain-displacement relations in the governing equations and separable method is adopted for these functions to get a set of ordinary differential equations in term of single variable, which are coupled. These displacement and rotational functions are approximated using cubic and quantic splines which results in to the system of algebraic equations with unknown spline coefficients. Incurring the boundary conditions with the algebraic equations, a generalized eigen value problem is obtained. This eigen value problem is solved numerically to find the eigen frequency parameter and associated eigenvectors which are the spline coefficients.The material properties of Kevlar-49/epoxy, Graphite/Epoxy and E-glass epoxy are used to show the parametric effects of the plates aspect ratio, side-to-thickness ratio, stacking sequence, number of lamina and ply orientations on the frequency parameter of the plate. The current results are verified with those results obtained in the previous work and the new results are presented in tables and graphs.

• Journal of Korean Tunnelling and Underground Space Association
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• v.7 no.3
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• pp.187-195
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• 2005

When a tunnel is excavated below groundwater table, the groundwater flow occurs towards the tunnel resulting in the seepage pressure. In this paper, the effect of groundwater flows on the behavior of shotcrete lining installed between ground-liner interfaces was studied considering permeability ratio between the ground and the shotcrete into account. Three-dimensional coupled finite element analysis was performed for this assessment. Seepage forces will seriously affect the shotcrete behavior since arching phenomena do not occur in seepage forces. A parametric study was conducted on the various tunnelling situations including interfacial properties between ground and shotcrete lining, the shape of tunnel cross-section and the thickness of liner, etc. Moreover, the convergence-confinement method (CCM) of a NATM tunnel considering seepage forces was proposed. The result showed that the more water tight is the shotcrete, the smaller is the convergence and the larger is the internal pressure. Therefore, the watertight fiber-reinforced shotcrete is found to be even more advantageous when used in under water tunnel.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.507-513
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• 2017

Bacillus subtilis spores can be used for protein display to engineer protein properties. This method overcomes viability and protein-folding concerns associated with traditional protein display methods. Spores remain viable under extreme conditions and the genotype/phenotype connection remains intact. In addition, the natural sporulation process eliminates protein-folding concerns that are coupled to the target protein traveling through cell membranes. Furthermore, ATP-dependent chaperones are present to assist in protein folding. CotA was optimized as a whole-cell biocatalyst immobilized in an inert matrix of the spore. In general, proteins that are immobilized have advantages in biocatalysis. For example, the protein can be easily removed from the reaction and it is more stable. The aim is to improve the pH stability using spore display. The maximum activity of CotA is between pH 4 and 5 for the substrate ABTS (ABTS = diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate). However, the activity dramatically decreases at pH 4. The activity is not significantly altered at pH 5. A library of approximately 3,000 clones was screened. A E498G variant was identified to have a half-life of inactivation ($t_{1/2}$) at pH 4 that was 24.8 times greater compared with wt-CotA. In a previous investigation, a CotA library was screened for organic solvent resistance and a T480A mutant was found. Consequently, T480A/E498G-CotA was constructed and the $t_{1/2}$ was 62.1 times greater than wt-CotA. Finally, E498G-CotA and T480A/E498G-CotA yielded 3.7- and 5.3-fold more product than did wt-CotA after recycling the biocatalyst seven times over 42 h.