• Title/Summary/Keyword: counting method

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A novel method for cell counting of Microcystis colonies in water resources using a digital imaging flow cytometer and microscope

  • Park, Jungsu;Kim, Yongje;Kim, Minjae;Lee, Woo Hyoung
    • Environmental Engineering Research
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    • v.24 no.3
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    • pp.397-403
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    • 2019
  • Microcystis sp. is one of the most common harmful cyanobacteria that release toxic substances. Counting algal cells is often used for effective control of harmful algal blooms. However, Microcystis sp. is commonly observed as a colony, so counting individual cells is challenging, as it requires significant time and labor. It is urgent to develop an accurate, simple, and rapid method for counting algal cells for regulatory purposes, estimating the status of blooms, and practicing proper management of water resources. The flow cytometer and microscope (FlowCAM), which is a dynamic imaging particle analyzer, can provide a promising alternative for rapid and simple cell counting. However, there is no accurate method for counting individual cells within a Microcystis colony. Furthermore, cell counting based on two-dimensional images may yield inaccurate results and underestimate the number of algal cells in a colony. In this study, a three-dimensional cell counting approach using a novel model algorithm was developed for counting individual cells in a Microcystis colony using a FlowCAM. The developed model algorithm showed satisfactory performance for Microcystis sp. cell counting in water samples collected from two rivers, and can be used for algal management in fresh water systems.

Study on Development of Automation System for Non-Contact Counting of ID Card (비접촉 ID카드 계수를 위한 자동화 시스템 개발)

  • Kang, Dae-Hwa;Hong, Jun-Hee;Guo, Yang-Yang;Lee, Hyok-Won
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.22 no.4
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    • pp.652-657
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    • 2013
  • In this study, we developed a counting method for non-contact ID cards using an optical fiber displacement sensor instead of the traditionally used friction counting method. The proposed method has the advantage of high speed and automated measurement. For counting non-contact ID cards, an H-type optical fiber sensor, jig part, and counting program are developed separately to build the system and adjust it. Through the experimental test results, it was confirmed that counting is possible with one type of international ID card and one type of financial security card based on ISO7810. Furthermore, by applying the proposed method to 100 ID cards 100 times repeatedly, it was confirmed that it has high accuracy and an error ratio of 0%. We experimentally demonstrated that the proposed counting method for non-contact ID cards using an optical fiber displacement sensor can perform measurements with high accuracy and high speed.

Fatigue life estimation using the multi-axial multi-point Load Counting method under Variable Amplitude Loading (가변진폭하중에서 다축-다점 하중 Counting method를 이용한 피로수명평가)

  • 이원석;이현우
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.11a
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    • pp.913-920
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    • 1996
  • In this study, the counting method for multi-axial and multi-point load states was proposed. Using this counting method, the load spectrum is generated from the service load history which is measured for boom structure of excavator. Loading state for loading points of boom structure is described as a multi-dimensional state space. From this load spectrum, the stress spectrum was generated by FEM analysis using the superposition of the unit load. The cumulated damage at the severe damage point of In nm structure by the failure example is calculated by Palmgren-Miner's rule. As a result of this study, the fatigue life estimation using the multi-axial and multi-point load counting method is useful.

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Counting People Walking Through Doorway using Easy-to-Install IR Infrared Sensors (설치가 간편한 IR 적외선 센서를 활용한 출입문 유동인구 계측 방법)

  • Oppokhonov, Shokirkhon;Lee, Jae-Hyun;Jung, Jae-Won
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2021.10a
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    • pp.35-40
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    • 2021
  • People counting data is crucial for most business owners, since they can derive meaningful information about customers movement within their businesses. For example, owners of the supermarkets can increase or decrease the number of checkouts counters depending on number of occupants. Also, it has many applications in smart buildings, too. Where it can be used as a smart controller to control heating and cooling systems depending on a number of occupants in each room. There are advanced technologies like camera-based people counting system, which can give more accurate counting result. But they are expensive, hard to deploy and privacy invasive. In this paper, we propose a method and a hardware sensor for counting people passing through a passage or an entrance using IR Infrared sensors. Proposed sensor operates at low voltage, so low power consumption ensure long duration on batteries. Moreover, we propose a new method that distinguishes human body and other objects. Proposed method is inexpensive, easy to install and most importantly, it is real-time. The evaluation of our proposed method showed that when counting people passing one by one without overlapping, recall was 96% and when people carrying handbag like objects, the precision was 88%. Our proposed method outperforms IR Infrared based people counting systems in term of counting accuracy.

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Accuracy of lntersection Counting Method in Measurement of Short Fiber Orientation Distribution by lmage Processing (화상처리에 의한 단섬유배향각 분포측정에 있어서 교점계수법의 정밀도)

  • 이상동;이동기;한길영;김이곤
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.04a
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    • pp.556-560
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    • 1996
  • In order to examine thd accuracy of intersection counting method, the fiber orientation distribution of simulation figure platted by PC is measured using image processing. The fiber orientation distribution obtained by an image processing method is compared with those by the intersection counting method. The result shows that the errors of the intersection counting method are large because its measurement is made by the cross point of the scanning line and the fiber.

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Accuracy of Intersection Counting Method in Measurement of Fiber Orientation Angle Distribution Using Image Processing (화상처리에 의한 섬유배향각 분포측정에 있어서 교차점합산법의 정밀도)

  • 이상동;박준식;이동기;한길영;김이곤
    • Journal of the Korean Society for Precision Engineering
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    • v.15 no.12
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    • pp.97-105
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    • 1998
  • The fiber oriented condition inside fiber reinforced composite material is a basic factor of mechanical properties of composite materials. It is very important to meausure the fiber orientation angle for the determination of molding conditions, mechanical characteristics, and the design of composite materials. In the work, the fiber orientation distribution of simulation figure plotted by PC is measured using image processing in order to examine the accuracy of intersection counting method. The fiber orientation function measured by intersection counting method using image processing is compared with the calculated fiber orientation function. The results show that the measured value of fiber orientation function using intersection counting method is lower than the calculated value, because the number of intersection between the scanning line and the fiber with smaller fiber aspect ratio is counted less than with larger fiber aspect ratio.

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Noise Reduction for Photon Counting Imaging Using Discrete Wavelet Transform

  • Lee, Jaehoon;Kurosaki, Masayuki;Cho, Myungjin;Lee, Min-Chul
    • Journal of information and communication convergence engineering
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    • v.19 no.4
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    • pp.276-283
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    • 2021
  • In this paper, we propose an effective noise reduction method for photon counting imaging using a discrete wavelet transform. Conventional 2D photon counting imaging was used to visualize the object under dark conditions using statistical methods, such as the Poisson random process. The photons in the scene were estimated using a statistical method. However, photons which disturb the visualization and decrease the image quality may occur in the background where there is no object. Although median filters are used to reduce the noise, the noise in the scene remains. To remove the noise effectively, our proposed method uses the discrete wavelet transform, which removes the noise in the scene using a specific thresholding method that utilizes photon counting imaging characteristics. We conducted an optical experiment to demonstrate the denoising performance of the proposed method.

A Segmentation Method for Counting Microbial Cells in Microscopic Image

  • Kim, Hak-Kyeong;Lee, Sun-Hee;Lee, Myung-Suk;Kim, Sang-Bong
    • Transactions on Control, Automation and Systems Engineering
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    • v.4 no.3
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    • pp.224-230
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    • 2002
  • In this paper, a counting algorithm hybridized with an adaptive automatic thresholding method based on Otsu's method and the algorithm that elongates markers obtained by the well-known watershed algorithm is proposed to enhance the exactness of the microcell counting in microscopic images. The proposed counting algorithm can be stated as follows. The transformed full image captured by CCD camera set up at microscope is divided into cropped images of m$\times$n blocks with an appropriate size. The thresholding value of the cropped image is obtained by Otsu's method and the image is transformed into binary image. The microbial cell images below prespecified pixels are regarded as noise and are removed in tile binary image. The smoothing procedure is done by the area opening and the morphological filter. Watershed algorithm and the elongating marker algorithm are applied. By repeating the above stated procedure for m$\times$n blocks, the m$\times$n segmented images are obtained. A superposed image with the size of 640$\times$480 pixels as same as original image is obtained from the m$\times$n segmented block images. By labeling the superposed image, the counting result on the image of microbial cells is achieved. To prove the effectiveness of the proposed mettled in counting the microbial cell on the image, we used Acinetobacter sp., a kind of ammonia-oxidizing bacteria, and compared the proposed method with the global Otsu's method the traditional watershed algorithm based on global thresholding value and human visual method. The result counted by the proposed method shows more approximated result to the human visual counting method than the result counted by any other method.

Pedestrian Counting System based on Average Filter Tracking for Measuring Advertisement Effectiveness of Digital Signage (디지털 사이니지의 광고효과 측정을 위한 평균 필터 추적 기반 유동인구 수 측정 시스템)

  • Kim, Kiyong;Yoon, Kyoungro
    • Journal of Broadcast Engineering
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    • v.21 no.4
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    • pp.493-505
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    • 2016
  • Among modern computer vision and video surveillance systems, the pedestrian counting system is a one of important systems in terms of security, scheduling and advertising. In the field of, pedestrian counting remains a variety of challenges such as changes in illumination, partial occlusion, overlap and people detection. During pedestrian counting process, the biggest problem is occlusion effect in crowded environment. Occlusion and overlap must be resolved for accurate people counting. In this paper, we propose a novel pedestrian counting system which improves existing pedestrian tracking method. Unlike existing pedestrian tracking method, proposed method shows that average filter tracking method can improve tracking performance. Also proposed method improves tracking performance through frame compensation and outlier removal. At the same time, we keep various information of tracking objects. The proposed method improves counting accuracy and reduces error rate about S6 dataset and S7 dataset. Also our system provides real time detection at the rate of 80 fps.

Comparative Study on Wave Induced Fatigue Analysis Methods for Steel Catenary Riser (파랑하중에 의한 Steel Catenary Riser 피로손상 평가 방법의 비교검토)

  • Lee, Jeong-Dae;Lee, Sung-Je;Jang, Chang-Hwan;Jun, Seock-Hee;Oh, Yeong-Tae
    • Journal of the Society of Naval Architects of Korea
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    • v.52 no.3
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    • pp.222-235
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    • 2015
  • The purpose of this study is to suggest guidelines for riser fatigue analysis in terms of selection of reasonable analysis method. Three analysis methods (spectral, regular wave, rain-flow counting) are introduced and compared. As the riser systems give non-linear response, the time-domain analysis method is more preferred than frequency-domain analysis method. The spectral fatigue analysis method, however, is still useful for identifying fatigue prone areas. Once stress RAO is established, fatigue damage can be calculated very quickly. The regular wave method and the rain-flow counting method are more time consuming but give more exact results compare to spectral method. In case of regular wave method, a set of regular waves which represent random sea states is considered for dynamic analysis. The rain-flow counting method is the most intuitive and exact method because it refers time history stresses containing most of non-linear effects of the riser system. However, it is not common for early design stage to use rain-flow counting method because of its high cost. In this study, it was confirmed that the regular wave method is the most cost effective way in specific cases. However, if the system is highly non-linear, it seems that the regular wave method gives less accurate results than rain-flow counting method. Therefore, it is imperative that the engineers select appropriate analysis method based on design stage and given engineering period. This paper also discusses the theoretical background of each calculation method and hydrodynamic aspects of marine riser systems. A steel catenary riser (SCR) line on FPSO was considered and marine dynamic program (OrcaFlex) was used for static and dynamic analysis.