• 한국약용작물학회지
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• 제21권4호
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• pp.262-267
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• 2013

The purpose of this study was to research for anti-oxidation and anti-wrinkle effects of Acar mono Sap (AM). To cosmetic effect of AM, safety effect (MTT assay), anti-wrinkle effect (elastase, MMP-1 inhibition assay) and anti-oxidant effect (DPPH assay) were measured. When water extract of AM was used for cell viability, it was over 100% at 6% (6 ml/100 ml in phosphate buffer) concentration. AM showed 45.7% elastase inhibition and 23.7% MMP-1 inhibition at 50% (50 ml/100 ml in phosphate buffer) concentration so that it had good anti-wrinkle characteristic. And AM showed 68.9% antioxidation capacity at 50% concentration by using a DPPH assay. Consequently, AM can be used as natural materials or additives for human skin owing to their beneficial biologic functions, including the anti-wrinkle effect, for cosmetic compositions.

• 대한화장품학회지
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• 제21권2호
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• pp.94-111
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• 1995

Paraben에 대한 Oil의 분배계수를 측정하고, 실제 에멀젼을 제조하여 방부력을 비교 측정하였다. 분배계수측정은 Hydrocarbon, Ester, Silicone Oil류로 분류하여 실험을 하였으며, Stirring Time, Temperature, Volume ratio에 따른 최적실험조건을 결정하여 실험을 수행하였다. 각각의 oil 종류에 따라 분배계수 값의 차이가 많았으며, 특히 Ester oil 류의 분배계수 값이 다른 오일 류에 비해 상당히 높음을 알 수 있었고, 오일의 Paraben에 대한 분배계수에 Propylene Glycol이 좋은 효과를 나타냄을 관찰하였다. 또한 각각의 오일을 주 처방으로 한 에멀젼을 제조하여, Challenge test에 주로 사용하는 5종의 미생물을 접종하여 균의 사멸속도를 비교한 결과, 분배계수가 낮은 오일을 함유한 에멀젼일수록 빠른 사멸속도를 보여주었다.

• 대한화장품학회지
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• 제23권3호
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• pp.128-133
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• 1997

In manufacturing emulsion lipstick in the past, heat and emulsifyoil phase made with oil, wax and lipophilic emulsifier with water phase together; and then mold above-mentioned materials, so-called lipophilic emulsified lipstick production method has been used. However it is hard to manufacture products using the above said method and furthermore, weak in moisturizing effect, lip care and stability. In this paper, I will discuss about multi-emulsion lipstick complementing already existing emulsion lipstick in terms of stability, giving moisturing effect of lip, and outstanding protection effectiveness of skin by safely gelatinating the state between amphiphilic lipid and water in stable.

• 대한본초학회지
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• 제25권4호
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• pp.145-149
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• 2010

Objectives : Since cosmetics have been one of the help of life, unlike medicine, natural products have been used for cosmetics, generally giving the image of safety and relief compared to synthetic products. Among them, Houttuynia cordata has been known as a useful herbal medicine with antibiotic, anti-inflammatory and anti-oxidative activities. In this study, we were to use Houttuynia cordata extract on formulation of cosmetic emulsions. Methods : The effects of Houttuynia cordata extract on emulsion stability and viscoelastic properties of emulsion were measured using turbiscan and rheometer. And we assessed the anti-oxidative and antibiotic activities of Houttuynia cordata extract. Results : 1. The results of this investigation for emulsion stability showed that the stability emulsion containing Houttuynia cordata extract was decreased depending on concentration of Houttuynia cordata extract. 2. Super oxide dismutase activity was strongly dependents on concentration of Houttuynia cordata extract. 3. Houttuynia cordata extract showed good anti-microbial activity against Staphylococcus aureus. Conclusions : Houttuynia cordata extract can be effectively used in cosmetic emulsions when the relation between natural product extracts and formulation of cosmetics is elucidated.

• 대한의용생체공학회:의공학회지
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• 제32권2호
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• pp.151-157
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• 2011

Tattooing is a performance for decorative and cosmetic marking by placing permanent ink into the skin. As the cultural meaning of tattoo in Korea is changing, the tattoo machines are widely spread n permanent cosmetic market. Though the use of the tattoo machine was increased, the evaluation standards of tattoo machine were not existed. Korea Food and Drug Association regulated the electrical and mechanical safety standards which were founded on the IEC 601-1 second edition. Also they regulated he biological safety standards which were derived from the ISO 10993 series, however, these general valuations of common medical device were insufficient for evaluating tattoo machine. We developed the standards of tattoo machine for safety and performance evaluation for tattoo machine by preliminary hazard analysis in ISO 14971. The evaluation criteria of tattoo machines are focused on the mechanical invasion. We suggested the additional evaluation items of the needle speed, length, vibration with general valuation criteria of common medical device. We anticipate that this research may be a primary stage to figure a standard regulation and evaluation for tattoo machine.

• Journal of Pharmaceutical Investigation
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• 제37권4호
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• pp.217-222
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• 2007

To develop cleansing tissue composed of silicone multiple emulsions which could easily remove make-up residues and confer skin protecting effect without damaging skin, we formulated various silicone multiple emulsions and evaluated the physicochemical properties including viscosity, cleansing effect, and safety effect. Also, cleansing tissue incorporated with silicone multiple (W/Si/W) emulsion was stored for 6 months, and judged its stability through microscopes under accelerated and long-term condition. Cleansing effect was evaluated by chromameter. Skin hydration effect was determined by corneometer and incorporation effect into non-woven fabric cotton was evaluated by volunteer survey. Low viscosity ranged from 400 centipoise (cP) to 1,000 cP was obtained from a stabilized W/Si/W emulsion containing more than 10% volatile silicone. Mean diameter of fresh W/Si/W emulsion was $20{\mu}m$, but after storage for 3 months at $45^{\circ}C$, the particle size of the W/Si/W emulsion increased up to $50{\mu}m$. Both W/Si/W emulsion-incorporated cleansing tissue and commercial product showed equally good cleansing effect. In addition, skin allergies such as erythema, edema, scaling itching, stinging, burning, tightness and prickling were not observed through macroscopic examination. From the transepidermal water loss results, the cleansing tissue consisting of W/Si/W emulsion showed superior hydration effect to commercial product. In conclusion, this study suggests cleansing tissue using W/Si/W emulsion could be used for an excellent efficacy compared with commercialized cleansing tissue.

• Korean Chemical Engineering Research
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• 제48권4호
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• pp.413-416
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• 2010

본 연구에서는 연근의 추출물에서 기능성화장품 소재를 개발하고자 하였다. 용매는 70~100%의 에탄올을 이용하였으며, 연근의 유효성분으로 nuciferine을 확인하였다. 연근 추출물의 화장품소재로서의 응용가능성을 보기 위해 안전성시험(MTT assay), 주름개선 효과시험(Elastase 활성저해 시험) 및 항산화효과시험(DPPH free radical scavenging assay)을 실시하였다. 100% 에탄올로 연근을 추출할 경우 $100{\mu}g/ml$의 농도에서 세포생존율이 80% 이상이어서 연근 추출물은 화장품소재로서 비교적 안전함을 알 수 있었다. 또한 연근 추출물은 $100{\mu}g/ml$의 농도에서 elastase 활성억제가 40~50%를 보여주어서 비교적 우수한 주름개선 효과를 나타내었다. 50% 항산화 저해능을 보이는 농도($FSC_{50}$)는 $5.0{\sim}38{\mu}g/ml$로 연근 추출물의 항산화효과가 매우 우수하였다. 따라서 연근 에탄올추출물은 주름개선 기능성화장품 소재로서 그 가능성이 높음을 알 수 있었다.

• 대한화장품학회지
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• 제42권3호
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• pp.279-284
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• 2016

연구에서는 수염에 대한 면도력을 향상시킬 수 있는 화장료 제형 개발을 위하여 대면용 화장료에서 사용가능한 우레아 및 여러 항산화제의 수염 강도 약화 효과를 연구하였다. 실제 모발 강도 약화 효과는 우레아 농도에 의존적인 성향을 보였으며, 모발 면적 증가율 역시 농도에 의존적인 것으로 나타나 우레아 농도에 따라 모발의 수화 정도가 달라지며 이것이 강도 약화에 영향을 주는 것을 확인할 수 있었다. 항산화제는 종류에 따라 소량으로도 뛰어난 강도 감소 효과를 보이며, 이는 모발에 다량 존재하는 시스틴 결합을 환원시키기 때문으로 해석된다. 마지막으로 대면용으로서의 적합성 판단을 위해 우레아 및 항산화제의 in vitro 안전성 테스트를 진행하였다. 안전성 테스트 결과 우레아는 대면용으로 사용하기 적합한 정도로 나타났으며, 항산화제 중에서는 글루타치온이 적합한 것으로 나타났다. 위 결과들을 통해 피부 자극이 적고 모발 강도 약화 효과가 뛰어나 면도용 제품에 적용하기 적합한 원료는 우레아 및 글루타치온임을 알 수 있었다.

• 대한화장품학회지
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• 제26권1호
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• pp.41-58
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• 2000

최근 환경오염으로 인한 지구오존층의 파괴로 자외선의 지표면의 도달 량이 증가하고 그로 인한 인간에 대한 피해가 증가하고 있다 . 과도한 자외선의 피부에 대한 직접적인 노출은 피부에 많은 문제점을 야기하므로 일차적으로 자외선차단제를 이용하여 인체에 대한 직접적인 피해를 최소화 해야한다 . 현재 자외선 차단제는 유기자외선흡수제와 무기자외선산란제가 많이 사용이 되는데 적은량으로도 효과가 높은 유기자외선차단제는 사용 시 주의를 요하므로 국가별로 사용량과 사용여부에 대하여 규제를 하고 있다. 본 연구에서는 플라보노이드류 ,알카로이드류 같은 자외선 흡수 성분을 함유한 식물중 UV/vis spectrophotometer 에서 자외선 흡수 peak을 나타내는 금은화 , 포공영, 녹차 , 황금추출물을 이용하여 화장품의 자외선 흡수제로서의 사용가능성여부를 조사하였다. 또한 식물추출물이 가진 자극성 성분과 오염성, 그들이 가진 유효성분들을 보호하고자 실리콘을 이용한 겔화를 시도하였다 . 자연친화적인 천연물로써 식물추출성분을 이용 유기자외선흡수제의 사용량을 줄이는 자외선홉수보조제로서 가능성을 보여주었다.

• 한국약용작물학회지
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• 제28권6호
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• pp.445-454
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• 2020

Background: Culturing wild ginseng adventitious root using plant factory technology provides genetic safety and high productivity. This production technology is drawing attention in the fields of functional raw materials and product development. The cultivation method using elicitors is key technology for controlling biomass and increasing secondary metabolites. Methods and Results: Elicitor treatments using methyl jasmonate, pyruvic acid, squalene, β-sistosterol were performed to amplify total ginsenosides (Rb1, Rc, Rb2, Rb3, and Rd) of cultured wild ginseng adventitious root. Thereafter, fermentation and steaming processes were performed to convert total ginsenosides into minor molecular ginsenosides (Rg3, Rk1, and Rg5). The result indicated that methyl jasmonate minimizes the reduction in fresh weight of cultured wild ginseng adventitious root and maximizes total ginsenosides (sum of Rb1, Rc, Rb2, Rb3, and Rd). Ginsenoside conversion results showed a maximum degree of conversion of 131 mg/g. Conclusions: In this study, we demonstrated that the optimal elicitor treatment method increased the content of total ginsenosides, while the steaming and fermentation processing method increased the content of minor ginsenosides.