Forty yeast strains isolated from soils taken from different locations in Egypt were tested for their P-solubilizing activities on the basis of analyzing the clear zone around colonies growing on a tricalcium phosphate medium after incubation for 5 days at $25^{\circ}C$, denoted as the solubilization index (SI). Nine isolates that exhibited P-solubilization potential with an SI ranging from 1.19 to 2.76 were genetically characterized as five yeasts belonging to the genus Saccharomyces cerevisiae and four non-Saccharomyces, based on a PCR analysis of the ITS1-26S region amplied by SC1/SC2 species-specific primers. The highest P-solubilization efficiency was demonstrated by isolate PSY- 4, which was identified as Saccharomyces cerevisiae by a sequence analysis of the variable D1/D2 domain of the 26S rDNA. The effects of single and mixed inoculations with yeast PSY-4 and Bacillus polymyxa on the P-uptake and growth of corn were tested in a greenhouse experiment using different levels of a phosphorus chemical fertilizer (50, 100, and 200 kg/ha super phosphate 15.5% $P_2O_5$). The results showed that inoculating the corn with yeast PSY-4 or B. polymyxa caused significant increases in the shoot and root dry weights and P-uptake in the shoots and roots. The P-fertilization level also had a significant influence on the shoot and root dry weights and P-uptake in the shoots and roots when increasing the P-level from 50 up to 200 kg/ha. Dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 200 kg/ha gave higher values for the shoot and root dry weights and P-uptake in the shoots and roots, yet these increases were nonsignificant when compared with dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 100 kg/ha. The best increases were obtained from dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 100 kg/ha, which induced the following percentage increases in the shoot and root dry weights, and P-uptake in the shoots and roots; 16.22%, 46.92%, 10.09%, and 31.07%, respectively, when compared with the uninoculated control (fertilized with 100 kg/ha).
Kim, Min Jeong;Shim, Chang Ki;Kim, Yong Ki;Hong, Sung Jun;Park, Jong Ho;Han, Eun Jung;Kim, Jin Ho;Kim, Suk Chul
The Plant Pathology Journal
/
v.31
no.3
/
pp.259-268
/
2015
This study investigated the chemical characteristics and microbial population during incubation of four kinds of aerated compost teas based on oriental medicinal herbs compost, vermicompost, rice straw compost, and mixtures of three composts (MOVR). It aimed to determine the effects of the aerated compost tea (ACT) based on MOVR on the growth promotion of red leaf lettuce, soybean and sweet corn. Findings showed that the pH level and EC of the compost tea slightly increased based on the incubation time except for rice straw compost tea. All compost teas except for oriental medicinal herbs and rice straw compost tea contained more ${NO^-}_3-N$ than ${NH^+}_4-N$. Plate counts of bacteria and fungi were significantly higher than the initial compost in ACT. Microbial communities of all ACT were predominantly bacteria. The dominant bacterial genera were analyzed as Bacillus (63.0%), Ochrobactrum (13.0%), Spingomonas (6.0%) and uncultured bacterium (4.0%) by 16S rDNA analysis. The effect of four concentrations, 0.1%, 0.2%, 0.4% and 0.8% MOVR on the growth of red leaf lettuce, soybean and sweet corn was also studied in the greenhouse. The red leaf lettuce with 0.4% MOVR had the most effective concentration on growth parameters in foliage part. However, 0.8% MOVR significantly promoted the growth of root and shoot of both soybean and sweet corn. The soybean treated with higher MOVR concentration was more effective in increasing the root nodule formation by 7.25 times than in the lower MOVR concentrations Results indicated that ACT could be used as liquid nutrient fertilizer with active microorganisms for culture of variable crops under organic farming condition.
Environmental concerns arising from synthetic herbicides in plant management systems have led to an interest in plant-derived compounds as natural herbicides. Inhibitory effects of compounds extracted with 50% methanol from corn (Zea mays L.) and pine (Pinus densiflora L.) were evaluated on large crabgrass (Digitaria sanguinalis (L.) Scop.), annual bluegrass Poa annua L.), radish (Raphanus sativus L.), and perennial ryegrass (Lolium perenne L.) The aqueous extracts inhibited seed germination and had postemergence activity on the four species. The stability of biological activity of corn grain, stover, and root extracts was not affected by heating to $135^{\circ}C$ or freezing/thawing treatments when applied at levels above 0.25kg m(sup)-2 based on dry weights of powders before extraction. Heating reduced the activity of pine litter and bark extracts at all levels except the highest application level but had little effect on pine needle extracts.
The productivity of cropping systems with corn and soybean were tested in field layouts such as monocultures of each, I-row alternation(l:1), 1-row corn with 2-rows soybean(l:2), and 1-row corn with 3 rows soybean(l : 3). Increasing soybean rows in alternative row croppings modified corn plant short and thick stalk. Decreasing soybean rows induced lodging of soybean plants because of lengthy stem. Although LAI in alternative row croppings increased by 2-8% over monocultures, light transmition into canopy was improved in alternative rows with rearrangement of leaf area and top dry wt.. Top/root ratio of corn plants in alternative rows was decreased as increasing soybean rows. The amount of total nitrogen uptake was increased by 4-22% in alternative row croppings over monocultures. Grain yield of corn plants was increased and that of soybean was decreased as rows of soybean increased in alternative row croppings. Land equivalent ratios were ranged from 0.96 to 1.01, but grain yields, amount of total nitrogen, and dry matter yields were increased by 17-20, 10-25, and 17-20%, respectively, in alternative rows. The 1 : 1 alternative row of corn and soybean was concluded the best cropping system for production of grain and dry matter.
Incubation condition affecting the chlamydospore formation and isolation from mycelia and conidia of Cylindrocarpon destructanse (isolate ACY-9701), isolated from the root rot lesion of the American ginseng (Panax quinquefolium) was investigated. Chlamydospores were formed from mycilia but not from conidia on the Czapek-Dox agar without carbon or nitrogen source after 20 days incubation at 2$0^{\circ}C$. In the medium added with nitrogen and carbon sources, immatured chlamy-dospore-like cells were formed from microconidia and mycelia as well. Immatured chlamydospore-like cells were formed from mycelia as well as microconidia In corn, kidney bean, and pea root extracts after 20 days incubation at 20"C, while typical chlamydospores were formed from both of them in the root extract of Panax quinquefolium. The 3.6 log chlamydospore/mm" was converted from microconidia in the medium, which was equal to 2.5% conidia formed. Under the light condition (251.1 pmol/m" sec, 12 hrs dark and light cycle), 4.2 log/mm" of chlamydospores were converted from interracially or terminal cells of macroconidia, which was 4.0% of macroconidia produced on Potato dextrose agar (PDA). When mycelia and microconidia were stored at -7$0^{\circ}C$ for 32 days and incubated on PDA after thawing at room temperature to isolate chlamydospores from them, microconidia and mycelia were still alive. Meanwhile, microconidial lysis was found after heating them at 32$^{\circ}C$ for 7 days, but the chlamydospores converted from macroconidia were not lysed up to 13 days at 32"C. to 13 days at 32"C.ot;C.
Three kinds of selection indexes were calculated and compared for effectiveness in selecting best performing hybrids from yield trial of corn. The following four characters were used in calculating indexes; yield, moisture content, and root and stalk lodging. 1. Among three indexes calculated, William's base index was more simple in calculation and more efficient in selection of a hybrid than other indexes. 2. Pesek and Baker's estimated index was considered less effective in this test, because of low estimation of genetic variance and covariances of characters from the limited materials and years of experiment. 3. Index calculated according to the Purdue University method (1976) was least effective, even though calculation was simple and easy. The less effectiveness of the index was due to the fact that equal importance of characters were given to each character without considering the differences in economic weight of each character. 4. Equations for expressing moisture content and root and stalk lodging in terms of market price were formulated and they were as follow : 1) Moisture content : $(Y-15.5){\times}$0.0065{\times}bushels/acre$, where Y was the average moisture content of a hybrid at the time harvest. The $0.0065 was the expenses required for removing one per cent of moisture from a bushel of shelled corn in 1976. 2) Root and stalk lodging:$Percent\;lodging{\times}price/Bu.{\div}50{\div}average$ yield of all entries in the test.
Korean Journal of Agricultural and Forest Meteorology
/
v.25
no.3
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pp.182-196
/
2023
Accurate and timely estimation of crop yields is crucial for various purposes, including global food security planning and agricultural policy development. Remote sensing techniques, particularly using vegetation indices (VIs), have show n promise in monitoring and predicting crop conditions. However, traditional VIs such as the normalized difference vegetation index (NDVI) and enhanced vegetation index (EVI) have limitations in capturing rapid changes in vegetation photosynthesis and may not accurately represent crop productivity. An alternative vegetation index, the near-infrared reflectance of vegetation (NIRv), has been proposed as a better predictor of crop yield due to its strong correlation with gross primary productivity (GPP) and its ability to untangle confounding effects in canopies. In this study, we investigated the potential of NIRv in estimating crop yield, specifically for corn and soybean crops in major crop-producing regions in 14 states of the United States. Our results demonstrated a significant correlation between the peak value of NIRv and crop yield/area for both corn and soybean. The correlation w as slightly stronger for soybean than for corn. Moreover, most of the target states exhibited a notable relationship between NIRv peak and yield, with consistent slopes across different states. Furthermore, we observed a distinct pattern in the yearly data, where most values were closely clustered together. However, the year 2012 stood out as an outlier in several states, suggesting unique crop conditions during that period. Based on the established relationships between NIRv peak and yield, we predicted crop yield data for 2022 and evaluated the accuracy of the predictions using the Root Mean Square Percentage Error (RMSPE). Our findings indicate the potential of NIRv peak in estimating crop yield at the county level, with varying accuracy across different counties.
Choi, Sung Won;Lee, Chang Sug;Park, Chang Hee;Kim, Dong Hee;Park, Sung Kwon;Kim, Beob Gyun;Moon, Sang Ho
Journal of The Korean Society of Grassland and Forage Science
/
v.34
no.4
/
pp.277-282
/
2014
Nutritive value analysis of feed is very important for the growth of livestock, and ensures the efficiency of feeds as well as economic status. However, general laboratory analyses require considerable time and high cost. Near-infrared reflectance spectroscopy (NIRS) is a spectroscopic technique used to analyze the nutritive values of seeds. It is very effective and less costly than the conventional method. The sample used in this study was a corn kernel and the partial least square regression method was used for evaluating nutrient composition, digestibility, and energy value based on the calibration equation. The evaluation methods employed were the coefficient of determination ($R^2$) and the root mean squared error of prediction (RMSEP). The results showed the moisture content ($R^2_{val}=0.97$, RMSEP=0.109), crude protein content ($R^2_{val}=0.94$, RMSEP=0.212), neutral detergent fiber content ($R^2_{val}=0.96$, RMSEP=0.763), acid detergent fiber content ($R^2_{val}=0.96$, RMSEP=0.142), gross energy ($R^2_{val}=0.82$, RMSEP=23.249), in vitro dry matter digestibility ($R^2_{val}=0.68$, RMSEP=1.69), and metabolizable energy (approximately $R^2_{val}$ >0.80). This study confirmed that the nutritive components of corn kernels can be predicted using near-infrared reflectance spectroscopy.
Kim, Kyung-Min;Jeong, Wu-Seong;Bhattarai, Rabin;Jeong, Han-Seok
Journal of The Korean Society of Agricultural Engineers
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v.64
no.3
/
pp.53-61
/
2022
This study evaluated the impacts of subsurface drainage design, i.e., spacing and depth, on agricultural productivity and environmental sustainability in two tile-drained fields (Sites A and E) under a corn-soybean rotation in the Midwestern United States. A calibrated and validated Root Zone Water Quality Model (RZWQM) was used to simulate Nitrate-N (nitrogen) losses to tile drainage and crop yields of 30 tile spacing and depth scenarios over 24 years (1992-2015). Our results presented that the narrower and deeper the tile drains are placed, the greater corn yield and Nitrate-N losses, indicating that the subsurface drainage design may cause a trade-off between agricultural productivity and environmental sustainability. The simulation results also presented that up to about 255.7% and 628.0% increase in Nitrate-N losses in Sites A and E, respectively, far outweigh the rate of increase in corn yield up to about 1.1% and 1.6% from the adjustment of tile spacing and depth. Meanwhile, the crop yield and Nitrate-N losses according to the tile configuration differed depending on the field, and the soybean yield presented inconsistent simulation results, unlike the corn yield, which together demonstrate the heterogeneous characteristic of agro-environmental systems to a subsurface drainage practice. This study demonstrates the applicability of agricultural systems models in exploring agro-environmental responses to subsurface drainage practices, which can help guide the introduction and installation of tile systems into farmlands, e.g., orchards and paddy fields, in our country.
Tolerance mechanism to simazine (6-chloro-N,N'-diethyl-1,3,5-triazine-2,4-diamine) in Coix lacryma-jobi was investigated with respect to herbicide detoxification via glutathione conjugation. Simazine was initially absorbed by seedlings of C. lacryma-jobi and corn, but after 12 hours of treatment, no significant difference in simazine absorption was found in both species. Simazine absorbed was rapidly metabolized to glutathione-simazine conjugate. One to six hours after treatment, metabolism was approximately 2-fold faster in C. lacryma-jobi than in corn. Glutathione content was found 1.5- and 2.3-fold higher in coleoptile and root of C. lacryma-jobi, respectively, compared with corn. In both species, the highest concentration of glutathione was found in coleoptile tissue. Glutathione S-transferase that exhibits activity with 1-chloro-2,4-dinitrobenzene was not significantly different between two species. However, glutathione S-transferase activity with simazine was approximately 2-fold greater in C. lacryma-jobi than in corn. The glutathione S-transferase activity was 20 to 30% greater in shoot of either species than in root. Fast protein liquid chromatography-anion exchange column was used to separate glutathione S-transferase isozymes in coleoptiles of C. lacryma-jobi and corn. A peak of glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene and two peaks of glutathione S-transferase activity with simazine from C. lacryma-jobi were coeluted with those from corn, but showed greater activity than in the case of corn. Another glutathione S-transferase isozyme that exhibits activity with simazine was detected in the elution of C. lacryma-jobi extract, but not in corn. Electron transport in chloroplast thylakoids isolated from leaves of both species was equally sensitive to simazine applied at 1 to 100 nM. These results indicate that the simazine tolerance in C. lacryma-jobi is due to its capacity to detoxify the herbicide via glutathione conjugation, which is positively correlated with the level of glutathione content and glutathione S-transferase activity.
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