• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.8 s.227
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• pp.1183-1189
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• 2004

A novel cell count method, which can improve the count efficiency and reduce contamination problem, was presented using micro lattice engraved on culture dish. The micro lattice has feature of $50{\mu}m{\times}50{\mu}m$ rectangular shape, $2{\mu}m$ line width, and $2{\mu}m$ depth in $3mm{\times}3mm$ area. In this paper, nickel mold was fabricated with thickness of 3mm and diameter of 80 mm, and transcription of the micro lattice on a polystyrene cell culture dish was performed by hot embossing at $200\;^{\circ}C$. The tedious and error-prone harvest/load processes of conventional cell counts with a hemocytometer could be omitted, and these advantages became magnified during periodical counts involving long-term cultures. SupT1 cells and HeLa cells were cultivated with the dish for 7 days in $CO_2$ incubator and counted as $371.84/mm^2$ and $123.36/mm^2$, respectively, during the cultivation without harmful effects on the cells.

• Biomedical Science Letters
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• v.6 no.1
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• pp.55-63
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• 2000

This study has been carried out to investigate the sensitivity of polymerase chain reaction (PCR) method over conventional acid-fast bacilli (AFB) staining and/culture methods for the detection of Mycobacterium tuberculosis from trace body fluid and paraffin-embedded tissues (PET) specimens. A total of 65 cases were employed for the AFB staining and culture test, and a total of 50 cases were subjected to PCR and histopathological analysis. Among the specimen showing negative reaction to AFB staining, 12.1% were positive to PCR and 3.7% of the specimen representing negative result to culture test showed positive reaction to PCR. In addition, 20.0% of the specimen with AFB negative showed positive reaction to PCR. From these results, it could be concluded that PCR method overwhelms AFB staining and culture tests in sensitivity and specificity to M tuberculosis detection.

• Journal of the Korean Society of Clothing and Textiles
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• v.19 no.2
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• pp.108-108
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• 1995

This study, on the American hippies and their fashion, is composed of an examination of the following; the social and cultural circumstances of America in the late 1960's, the charac? teristics of the hippies that appeared in the midst of the foresaid background, how those factors materialized in the hippies' distinctive fashion and how they influenced tahion to come. The hippies were composed of the young generation that revolted against all general conventional values of American society and seceded from it, seeking for the ideas of love and freedom. They expressed their newly defined culture-rock music based on drug culture, psychedelic art, rejection of an established society and ethnic tastes that sprang up from nostalgia concerning nature-by means of their own distinctive fashion. And as a revival of the hippies' fashion, neo-hippie and grunge fashions newly appeared in the F /W season of 1992 and afterwards, adding fresher and more abundant sensibility to the original image and layered look of the hippies. The hippies' fashion has not only played a significant part in modem fashion history but will continue to exert its influence in the 1990's as the hippies generation are presently the center of American culture and leaders of world culture. In conclusion, various sorts of "individ- uality" and "the pleasure in the wearing itself" expressed by the original layering of the hippies' fashion presents new promise in the coming development at fashion.

• Journal of the Korean Society of Clothing and Textiles
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• v.19 no.2
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• pp.278-286
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• 1995

This study, on the American hippies and their fashion, is composed of an examination of the following; the social and cultural circumstances of America in the late 1960's, the charac\ulcorner teristics of the hippies that appeared in the midst of the foresaid background, how those factors materialized in the hippies' distinctive fashion and how they influenced tahion to come. The hippies were composed of the young generation that revolted against all general conventional values of American society and seceded from it, seeking for the ideas of love and freedom. They expressed their newly defined culture-rock music based on drug culture, psychedelic art, rejection of an established society and ethnic tastes that sprang up from nostalgia concerning nature-by means of their own distinctive fashion. And as a revival of the hippies' fashion, neo-hippie and grunge fashions newly appeared in the F /W season of 1992 and afterwards, adding fresher and more abundant sensibility to the original image and layered look of the hippies. The hippies' fashion has not only played a significant part in modem fashion history but will continue to exert its influence in the 1990's as the hippies generation are presently the center of American culture and leaders of world culture. In conclusion, various sorts of "individ- uality" and "the pleasure in the wearing itself" expressed by the original layering of the hippies' fashion presents new promise in the coming development at fashion.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.357-369
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• 2010

Cymbidium is horticulturally important and has been one of the most commercially successful orchid plants as well as cut flowers around the world including Korea. Up to now, a huge number of elite Cymbidium cultivars have been released on the commercial market via cross-hybridization, mutation and polyploidization breeding techniques. To investigate on breeding system in Cymbidium, we inquired the brief history and techniques of breeding and the current status on Cymbidium breeding in Korea. Also, the general propagation process of elite Cymbidium lines via tissue culture should be presented. However, the slow process of conventional breeding and the lack of useful genes in Cymbidium species delays the introduction of new cultivars to the commercial market. To solve these limitations, efficient regeneration and genetic transformation systems should be established in the improvement of Cymbidium breeding program. During the last several decades, some progress has been made in tissue culture and genetic transformation in Cymbidium species. We review the recent status of tissue culture and genetic transformation systems in Cymbidium plants.

• Korean Linguistics
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• v.79
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• pp.145-169
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• 2018

The purpose of this paper is to review critically the validity and relevance of the pan-national language culture improvement movement, Annyeong! Urimal(Hello! Our Language) implemented for the last three years as part of the government-driven projects, and to seek for improvement direction or fundamental reorientation of this movement. In order to accomplish this purpose, this paper firstly points out abstract and conventional aspects of the diagnosis for background and the purpose of projects, and suggests that this movement focuses on verbal abuse rather than language destruction and pays attention to positive aspects of newly rising language use. Then, with reference to main agent, this paper argues that the government must lead actively formation of voluntary nongovernmental agent with indirect financial and institutional support. Thirdly, it is suggested that public language should be the top priority target sphere and the language used by the leaders of society and the older generation should be also included in the target spheres of this movement. Finally, this paper indicates that enlightenment/public relation projects end often in one time events, imposing unilaterally the normative contents, and also emphasizes the importance of survey/research projects and deliberation/evaluation projects.

• Korean Journal of Medicinal Crop Science
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• v.20 no.4
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• pp.217-221
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• 2012

This research was conducted to investigate the influence of various organic substrates on growth and yield of ginseng seedling grown organically in the closed plastic house. The pH and EC of substrates used for organically ginseng cultivation ranged 5.93~6.78 and 0.03~0.15 dS/m respectively. The concentrations $NH_4$-N and $NO_3$-N respectively was 14.01~68.63 mg/L, 5.60~58.83 mg/L. The average quantum of the closed plastic house was range from 10 to 16% of natural light. In July and August, the maximum temperature of the closed plastic house did not exceed 30 and the average temperature was maintained within 25 lower than the field because air conditioning ran. The PPV-1 and PPV-2 bed soil substrates produced higher stem length, stem diameter, shoot fresh weight and leaf area than those of conventional culture. In PPV-2 bed soil substrates, root fresh weight and root diameter was the highest. The root fresh weight of PPV-2 bed soil substrates in closed plastic house was maximum 25% heavier than the conventional cultivation. The results of this experiment will be utilized for making new substrate application for organic ginseng culture in the plastic house.

• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.67-74
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• 2005

To overcome various disadvantages of conventional cul-ture vessels for micropropagation, a novel disposable vessel, the 'Vitron', made of a multi-layered $OTP^{(R)}$ film and supported by a polypropylene frame, was developed. The film possesses superior properties such as: high light transmittance, low water vapor transmittance and thermal stability and in particular, high gas-permeability. Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in Vitron and polycarbonate vessels on $3\%$ sugar-containing agar on MS medium and placed at 3000 ppm $CO_2$-enrichment at a low photosynthetic photon flux density (PPFD) ($45{\mu}mol\;m^{-2}\;s^{-1}$). The in vitro and ex vitro growth, and the net photosynthetic rate of in vitro and ex vitro plantlets were significantly enhanced in the Vitron compared to those cultured in a polycarbonate vessel. Explants that were cultured on the same MS medium under low PPFD at various $CO_2$ concentrations were also cultured at 3000 ppm $CO_2$- enrichment at various PPFD: 30, 45, 60, 75 and $90{\mu}mol\;m^{-2}\;s^{-1}$. The best in vitro and ex vitro growth obtained for 3000 ppm $CO_2$-enrichment at $75{\mu}mol\;m^{-2}\;s^{-1}$ PPFD. The novel Vitron vessel, when placed under the two conditions, may replace conventional culture vessels for the successful micropropagation of sweetpotato.

• Journal of the Korea Furniture Society
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• v.22 no.3
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• pp.190-198
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• 2011

This study intended to present a new direction of differentiated and innovative furniture design for small living space overcoming the uniformity of conventional small living. In order to achieve these goals, a new piece of furniture with multi functions useful for such small space has been designed and explained in this study. This new type multi-functional furniture can be utilized as kitchen, dining table, home bar or office stand depending on the user's necessity. In particular, it provides more adaptability to changing life and spatial availabilities for small living space in terms of space division, separation and fusion features. Based on the needs of small living space brought by social changes, the conventional concept of small living space, which divides space by partitions or walls, should be changed into flexible perception of space. Also, this research proposed the features of furniture design for functional shifts in terms of its functional, structural, and systematical aspects. In this respect, space can be regarded as a variable place and the image of space can be differently appreciated by means of shift, functional transition and mobility. That is, small living space can be perceived as kitchen, living space or working space at the same time. In conclusion, the versatile use of limited space can be a solution to the issues related to small living space; the convertible and movable furniture called F4 Island presented in this study can transform such spatially limited space into a space with multiple functions to meet the requirements of its residents.

• The Journal of the Convergence on Culture Technology
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• v.2 no.2
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• pp.29-35
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• 2016

The conventional educational robots, which are commonly industrial robots or toy robots, use text-based programming to teach the students. Therefore, students have difficulty in studying robotics due to the difficulties of text based language. The developed robot in this study have a camera, which have the color tracking function, and it has various sensors and actuators. It supports the open hardware and uses graphic language based programming. The developed educational robot is programmed by Scratch, which uses graphic modular language. We also present a curriculum, which is based upon the developed robot and Scratch. We applied the robot and curriculum to the primary school students. We obtained satisfactory results comparing it with the conventional robot education. Furthermore, the imagination and execution ability of students showed enhancement in learning robotics. Thus, this fact means the validity and effectiveness of the proposed approach.