• Korean Journal of Food Science and Technology
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• v.7 no.4
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• pp.229-237
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• 1975

An attempt was made to utilize the enzyme produced by Asp. oryzae as meat tenderizer. The production, purification, and various properties of proteinase produced by Asp. oryzae were investigated. Results obtained are as follow; 1. A strain which had the highest proteolytic activity was selected among 9 Aspergillus species. 2. Culture medium consisted of wheat bran 10g, 2% glucose, 0.03% urea and 0.1% $MgSO_4$ (pH 6.5). Mold was incubated at $30^{\circ}C$ for 3 days. 3. Enzyme extract from culture medium were fractionated with ammonium sulfate and purified by Sephadex G-75 column chromatography. 4. When pH of reaction mixture was controlled, maximal activity of proteinase by Asp. oryzae was obtained at pH 3, pH 6.6, $8.4{\sim}8.5$ and pH 10.0 to 10.5. Those results were interpreted to show that enzyme consists of acid proteinase, neutral proteinase and alkaline proteinase. Enzyme was stable at pH 6 to 10. 5. Opt. temperature for proteinase activity was $50^{\circ}C$, but enzyme was stable up to $40^{\circ}C$. 6. The proteinase was inhibited by $Ag^+$. It was also inhibited by EDTA. 7. When myofibrillar proteins were treated by proteinase from Asp. oryzae, ATPase activities of myofibrillar proteins changed remarkably. Accordingly, it was concluded that proteinase produced by Asp. oryzae were able to be used as meat tenderizer.

• Neonatal Medicine
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• v.15 no.1
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• pp.44-53
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• 2008

Purpose : Epidemic keratoconjunctivitis (EKC) caused by adenovirus is a highly contagious disease, which has been reported as outbreaks involving adults in the community. However, there has been no report on EKC outbreak by adenovirus in a neonatal intensive care unit (NICU) in Korea. Aims of this study were to investigate the EKC outbreak by adenovirus type 8 in NICU and to confirm an effectiveness of polymerase chain reaction (PCR) for diagnosis. Methods : Conjunctival swab or nasopharyngeal aspirate specimens were taken from all patients and tested by viral culture and PCR. Adenovirus serotype was determined by sequencing of PCR product of selected region of hexon gene using the virus isolates or specimens. Results : An outbreak of EKC occurred which was involving 12 preterm infants in the NICU of the Seoul National University Children's Hospital between July 12th and August 1st, 2005. Three hospital staffs and one family member of the neonate were also affected. Adenovirus was detected in 12/12 (100%), 6/11 (54.5%) by PCR and virus culture, respectively. Eleven PCR-positive neonates were identified as serotype 8 by sequencing. The first affected 4 babies have had routine ROP (retinopathy of prematurity) examinations one week ago. While previous outbreaks were sustained for a few months, the event in our unit was controlled without complications in 3 weeks. Conclusion : We analyzed the EKC outbreak by adenovirus type 8 in NICU. Adenovirus serotype was identified by PCR and sequencing with high sensitivity for the first time in Korea, so we suggest this method can be very useful for rapid diagnosis and infection control.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.492-500
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• 2017

The purpose of this study was to investigate the seasonal distribution of phytoplankton as prey for oysters and to characterize the environmental factors controlling their abundance from June 2016 to May 2017, in the northeast coast between Tongyeong and Saryang Island, particularly for the oyster farming area. During the survey period, water temperature changed from $7.54^{\circ}C$ in February to $29.5^{\circ}C$ in August. The abnormal high temperature persisted during one month in August. Salinity was low due to summer rainfall and typhoon. The lowest level was 30.68 psu in September, and it peaked at 34.24 psu in May. The dissolved oxygen (DO) concentration ranged from $6.0-9.45mg\;L^{-1}$, and the DO concentration in the surface layer was like that in the bottom layers. The seasonal trends of pH were also like those of DO. The pH ranged from 7.91 to 8.50. Nitrate with nitrite, phosphate, and silicate concentrations ranged from $0.14{\mu}M$ to $7.66{\mu}M$, from $0.01{\mu}M$ to $4.16{\mu}M$, and from $0.27{\mu}M$ to $20.33{\mu}M$, respectively. The concentration of chlorophyll a (Chl. a) ranged from $0.37{\mu}g\;L^{-1}$ to $2.44{\mu}g\;L^{-1}$ in the surface layer. The annual average concentration was $1.26{\mu}g\;L^{-1}$. The annual mean phytoplankton community comprised Bacillariophyta (69%), Dinophyta (17%), and Cryptophyta (10%), respectively. Dinoflagellate Prorocentrum donghaiense in June was the most dominant at 90%. In the summer, diatom Chaetoceros decipiens, Rhizosolenia setigera and Pseudo-nitzschia delicatissima were dominant. These species shifted to diatom Chaetoceros spp. and Crytophyta species in autumn. In the winter, high densities of Skeletonema spp. and Eucampia zodiacus were maintained. Therefore, the researchers thought that the annual mean Chl. a concentration was relatively lower to sustain oyster feeding, implying that the prey organism (i.e., phytoplankton) was greatly controlled by continuous filter feeding behavior of oyster in the vicinity area of the oyster culture farm.

• (The) Research of the performance art and culture
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• no.23
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• pp.63-103
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• 2011

This paper inquires closely into the background of pansori gosu(drummer) and his social position based on documents and oral materials and the performance features of gosu in the entire process of pansori performance, 'preparation', 'performance' and 'aftermath', focused on the relationship between the changja(singer)-gosu. In the past, some gosus were ex-tightrope performers. Their social position and working conditions were better than that of the ex-tightrope performers but were worse than that of the pansori singer. After 1910's, people formed some special sense about the gosu due to the change of the space for pansori performances and the technological advances on the media, and gosu's conditions improved. The theory of pansori drum gradually began to be established well. The function and the role of gosu in the whole process of pansori performance may be summarized as follows. To begin with, the training with various and a lot of singers is required in the 'preparation'. Rehearsals are divided into individual practices and joint practices, and the latter can be controlled by the level of the capacity of gosu and the degree of the experience between chanja-gosu. Next, bobiwi(flattering drumming) and chuimsae(encouraging remarks) are important in tbe 'process'. The gosu has to share the speed of one jangdan(rhythmic patterns) and the accent of the sori and adjust his enery. Besides, he has to acknowledge the naedeureum(beginning sign) and reply with changja's singing. In formal performances, working in harmony with changja and gosu and their joint experiences are necessary for the gosu; in pansori contests, giving chanja a stability; in contests for gosu, drumming skill, position, chuimsae; in small performances and new adaptation of pansori, cheap fees and positive response of the transformational play or ad-lib; in lecturer concerts, reacting quickly to rapidly changing situations. Chuimsae is way which gosus and audiences express their feeling together, however, its context and sound are different. Finally, 'aftermath' is a process the pair of chamgja and gosu mutually evaluates about performance or audiences estimate that.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.39-46
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• 2015

Objective of this research was to investigate the influence of compositions and concentrations of fertilizer solutions on the vegetative growth and nutrient uptake of 'Seolhyang' strawberry. To achieve this, the solutions of acid fertilizer (AF), neutral fertilizer (NF), and basic fertilizer (BF) were prepared at concentrations of 100 or $200mg{\cdot}L^{-1}$ based on N and applied during the 100 days after transplanting. The changes in chemical properties of the soil solution were analysed every two weeks, and crop growth measurements as well as tissue analyses for mineral contents were conducted 100 days after fertilization. The growth was the highest in the treatments with BF, followed by those with NF and AF. The heaviest fresh and dry weights among treatments were 151.3 and 37.8 g, respectively, with BF $200mg{\cdot}L^{-1}$. In terms of tissue nutrient contents, the highest N, P and Na contents, of 3.08, 0.54, and 0.10%, respectively, were observed in the treatment with NF $200mg{\cdot}L^{-1}$. The highest K content was 2.83%, in the treatment with AF $200mg{\cdot}L^{-1}$, while the highest Ca and Mg were 0.98 and 0.42%, respectively, in BF $100mg{\cdot}L^{-1}$. The AF treatments had higher tissue Fe, Mn, Zn, and Cu contents compared to those of NF or BF when fertilizer concentrations were controlled to equal. During the 100 days after fertilization, the highest and lowest pH in soil solution of root media among all treatments tested were 6.67 in BF $100mg{\cdot}L^{-1}$ and 4.69 in AF $200mg{\cdot}L^{-1}$, respectively. The highest and lowest ECs were $5.132dS{\cdot}m^{-1}$ in BF $200mg{\cdot}L^{-1}$ and $1.448dS{\cdot}m^{-1}$ in BF $100mg{\cdot}L^{-1}$, respectively. For the concentrations of macronutrients in the soil solution of root media, the AF $200mg{\cdot}L^{-1}$ treatment gave the highest $NH_4$ concentrations followed by NF $200mg{\cdot}L^{-1}$ and AF $100mg{\cdot}L^{-1}$. The K concentrations in all treatments rose gradually after day 42 in all treatments. When fertilizer concentrations were controlled to equal, the highest Ca and Mg concentrations were observed in AF followed by NF and BF until day 84 in fertilization. The BF treatments produced the highest $NO_3$ concentrations, followed by NF and AF. The trends in the change of $PO_4$ concentration were similar in all treatments. The $SO_4$ concentrations were higher in treatments with AF than those with NF or BF until day 70 in fertilization. These results indicate that compositions of fertilizer solution should to be modified to contain more alkali nutrients when 'Seolhyang' strawberry is cultivated through inert media and nutri-culture systems.

• Journal of the Korean Society of Food Culture
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• v.18 no.6
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• pp.551-561
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• 2003

To determine the conditions of the fermentation and storage for Chonggak kimchi in kimchi refrigerator, prepared Chonggak kimchi took into kimchi refrigerators which were controlled at four different modes of the fermented temperature and time, and fermented and kept for 16 weeks. The pH in Chonggak kimchi fermented at $20^{\circ}C$ for 24 hours/stored at $-1^{\circ}C$ dropped greater than all of kimchi fermented at other combinations, and the changes of pH at any combinations were not greater than those in Baechu kimchi, because pH in Chonggak kimchi did not dropped below 4.5. Acidities in Chonggak kimchi were greatly increased at higher temperature. The acidity in Chonggak kimchi during the first week of fermentation was lower than that in Baechu kimchi and then it was rather higher because of the addition of waxy rice paste. In texture, puncture force of Chonggak kimchi was decreased slowly until 8 weeks of fermentation and then did not changed much and the highest values showed in Chonggak kimchi stored directly at $-1^{\circ}C$ without any fermentation. In sensory evaluation, the scores for the carbonated flavor and the sourness were the highest in Chonggak kimchi fermented at $20^{\circ}C$ for 24 hours/stored at $-1^{\circ}C$, but the lowest in Chonggak kimchi stored directly at $-1^{\circ}C$ without any fermentation because of some undesirable flavors. The lowest hardness showed in Chonggak kimchi fermented at highest temperature and the best hardness was in Chonggak kimchi fermented at $5^{\circ}C$ for 3 days or 6 days/stored at $-1^{\circ}C$. The appearance was the best in Chonggak kimchi fermented at $20^{\circ}C$ for 24 hours/stored at $-1^{\circ}C$ and the worst was in Chonggak kimchi stored directly at $-1^{\circ}C$ without any fermentation. The overall acceptability of Chonggak kimchi fermented at $20^{\circ}C$ for 24 hours/stored at $-1^{\circ}C$ was good after 4 weeks of fermentation, but in Chonggak kimchi fermented at $5^{\circ}C$ for 3 days or 6 days/stored at $-1^{\circ}C$ it was good after 6 weeks. Total microbial counts in most of Chonggak kimchi were reached to a maximum number within 7 days, and then decreased similarly at all modes. Leuconostoc spp. and Lactobacillus spp. increased to maximum number of $1.48{\times}10^9\;and\;5.62{\times}10^9$, respectively, in Chonggak kimchi fermented for 7 days. Yeast counts showed a increasing trend not depends on fermenting temperature and they were lower counts than those in Baechu kimchi. Waxy rice paste which added to Chonggak kimchi resulted in increasement of glucose as a carbon source and stimulated to reproduce the microbes in Chonggak kimchi.

• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.276-280
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• 2012

This study was conducted to determine the ideal beginning date of plastic house optimum heating for off-season (December) production of asparagus. To achieve this, the 1.6-year-old 'Green tower' asparagus was cultivated in Jeju area ($33^{\circ}$28.110N, $126^{\circ}$31.076E) and the yield and quality among 7 different beginning dates of heating from Oct. 25 to Jan. 20 with 15-days interval were compared. The heating was controlled to maintain the inside temperature was higher than $20^{\circ}C$, But the plastic house was ventilated when the inside temperature rose to $30^{\circ}C$. Days to sprouting took longer as heating dates were delayed. Days to sprouting took 52 days in heating at December 30, and the days were shortened thereafter. As the beginning of heating was delayed, sprouting of asparagus was retarded. Among treatments, days to sprouting was 52 days and longest when the heating began on December 30. When the heating began on October 25, harvest was earliest and started on 7 November. When the heating began on November 15 and 30, harvest started on November 30 and December 18, respectively. Harvesting started on November 7 for October 25 heating, November 30 for Nov. 15 heating, and December 18 for November 30 heating, respectively. When the heating began was done after December. 15, harvesting was possible after early January. The number of spear, spear weight and yield were highly increased in the treatments of heating after January. 10 when the asparagus dormancy was broken. In the case of heating dates were before the asparagus dormancy breaking, the total yield was highest in November 15 heating with 607 kg/10a. The marketable yield of November 15 heating was 386kg/10a, which was twice higher than the 193 kg/10a of October 25 heating. Accordingly, it is recommended to start heating on November 15 for the production of off-season (December) asparagus in Jeju. Therefore, the optimum heating date to start was November 15 for the improvement of quality and yield of off-season (December) asparagus in Jeju area.

• The Korean Journal of Mycology
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• v.27 no.1 s.88
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• pp.10-14
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• 1999

A single phase composter was constructed by modifying the conventional mixer of sawdust for the cultivation of oyster mushroom Pleurotus ostreatus. The machine was designed on the basis of 3-phase-1 system which was controlled in prewetting, pasteurization and fermentation processes. In composting 200 kg of straw and cotton waste in the machine, it took 20 minutes in prewetting step and also to hours at $65^{\circ}C$ in pasteurization process. Postfermentation by aerothermophiles was completed by treating the compost at $45^{\circ}C-50^{\circ}C$ for 48 hours which was shorten 24 hours from the conventional method. In the postfermentation at high temperature, forced aeration and/or vigorous mixing process(es) played a great role in the improvement of spawn quality. The growth of mycelium of oyster mushroom was excellent in the culture combinated with 3 parts of surface inoculation and 7 parts of mechanical mixing.

• Journal of Sericultural and Entomological Science
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• v.16 no.1
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• pp.1-20
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• 1974

These studies are conducted to find out inducing factors for the non-sprouting bud which appears in the utility reclaimed hill mulberry held and to establish control method for it since 1971. The results are as follows： 1. In spring, winter bud does not germinate at all on the top of branches or does wither suddenly after or during its germination. Necrosis and browning are appeared in cortex and phloem of non-sprouting bud branch. In autumn, the deterioration of leaves, the abnormal leaves on the top of branch, and browning of veins or petioles are also observed. 2. The soil of non-sprouting bud mulberry Held were found to be sand loom derived from granite rocks. The sub soil of it was very poor in development of root system because it had hard soil and lower pore space ratio. Especially, the sub soil to bring about severe non-sprouting bud had been easily appeared with deficiency of moisture content because of high density solid. 3. Content of soil moisture was significantly lower in the severe non-sprouting bud soil than in the healthy field. The sub soil o( the healthy field contained proper moisture content. On the other hand, the sub soil of the severe non-sprouting bud held contained almost critical moisture content for wilting. 4. The depth of available soil was shallow in the non-sprouting bud than in the healthy fold. The more rate of the non-sprouting bud was severe, the more available soil depth was sallow. 5. Available boron content in soil was affected by moisture content in soil. There was. lower moisture content in the non-sprouting bud field than that in healthy fold during 5, June to 5, September. 6. There was no significant correlation between soil pH and available boron content. On the other hand, the correlation of the content of organic matter and available boron content appeared to be highly positive significance. 7. The quantity of boron was significantly contained more in healthy mulberry field (0.34∼0.43ppm) than in non-sprouting bud field (0.10∼0.28ppm). 8. Boron content in leaves and barks was significantly lower in the non-sprouting bud trees than in healthy trees. 9. The symptom of non-sprouting bud induced from boron free sand culture was similar with that arised in the non-sprouting bud field. 10. The rate of non-sprouting bud was high by the increased application of lime. Considering the facts mentioned the above, author may conclude that the non-sprouting bud of mulberry tree is caused by boron deficiency, but also it is affected by the parent rocks, organic matter, soil moisture content and lime application. 11. The non$.$sprouting bud may be completely controlled by the application of 6∼9kg borax per 10a mulberry field twice a year in spring and summer.

• Clinical and Experimental Reproductive Medicine
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• v.33 no.4
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• pp.237-243
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• 2006

Objective: The aim of this study was to evaluate the efficacy of frozen-thawed ET in poor prognosis patients such as the old age (38~44 years; OA group) and the patients who did not achieve clinical pregnancy with the first fresh ET cycle (non-pregnant patients; NP group). Methods: Laboratory and clinical data were collected from fresh and frozen-thawed ET cycles of OA and NP group. Controlled ovarian hyperstimulation (COH) and conventional insemination or ICSI, in vitro culture and ET were performed by routine procedures. Supernumerary embryos were frozen by the slow freezing method, and frozen embryos were thawed by the rapid thawing method. Embryo development, pregnancy and implantation rates were statistically analyzed by Student t-test and chi square test Results: Mean ages were similar between fresh ET ($40.0{\pm}1.8$ years, n=206) and frozen-thawed ET ($39.9{\pm}1.9$ years, n=69) cycles in OA group. However, the clinical pregnancy and implantation rate of subsequent frozen-thawed ET significantly higher than those of fresh ET cycles (29.0% and 11.2% vs. 16.5% and 7.0%, p<0.05). In NP group, there was no difference in the mean age between fresh ET ($31.2{\pm}2.3$ years, n=40) and frozen-thawed ET ($31.9{\pm}3.1$ years, n=119) in subsequent cycles. The clinical pregnancy and implantation rates were similar between the subsequent fresh ET (42.5% and 22.6%) and the frozen-thawed ET (40.3% and 18.8%). Conclusion: In old age patients, higher pregnancy rate of frozen-thawed ET compared to fresh ET cycles in this study. It may be related that better uterine environments for implantation in frozen-thawed ET cycles than that of non-physiological hormonal condition in uterus of fresh COH cycles.