• Title/Summary/Keyword: controlled culture

Search Result 513, Processing Time 0.028 seconds

Sexual Maturation Inducement of Striped Knife-Jaw, Oplegnathus fasciatus by Manipulating Environmental Condition (환경조절에 의한 돌돔 Oplegnathus fasciatus 성 성숙 유도)

  • Kim, Sung-Yeon;Bang, In-Chul;Kim, Seok-Min
    • Korean Journal of Ichthyology
    • /
    • v.12 no.1
    • /
    • pp.46-53
    • /
    • 2000
  • Artificial gonadal maturation and spawning inducement of striped knife-jaw Oplegnathus fasciatus were studied. Effects of water temperature and photoperiod under different regims on gonadal activity and maturation of three years old O. fasciatus were investigated histologically. In experiment I (Exp. I), water temperature was gradually increased from $14.5^{\circ}C$ to $21.0^{\circ}C$ and photoperiod was also gradually increased from 10 : 30 L to 15 : 30 L from December 1996 to February 1997 and this conditions were maintained till April. In experiment II (Exp. II), water temperature was increased in the same way from Exp. I and photoperiod was controlled as natural condition till early March and then increased to 15 : 30 L immediately. Control fish were reared in net-cage culture system in the sea from December 1996 to April 1997. Gonadal activity was initiated by increasing water temperature in both Exp. I and II from January. In Exp. I, gonadal maturation and spawning were induced from February when water temperature and photoperiod reached at $21.0^{\circ}C$ and 15 : 30 L, respectively. In Exp. II, complete gonadal maturation was not induced until early March but after treated by compensatory long photoperiod (15 : 30 L), the gonad was matured and subsequently spawning occurred.

  • PDF

Effects of Light Emitting Diodes on Growth and Morphogenesis of in vitro Seedlings in Platycodon grandiflorum (도라지 배양묘의 생장 및 형태형성에 미치는 발광다이오우드의 효과)

  • 은종선;김영선;김용현
    • Korean Journal of Plant Tissue Culture
    • /
    • v.27 no.1
    • /
    • pp.71-75
    • /
    • 2000
  • To clarify the possibility of plant production under red, green. blue, and red+blue using light emitting diodes (LEDs) and fluorescent lamps (control), the effects of light quality on the growth and morphogenesis of in vitro seedlings in Piatycodon grandiflorum were examined. The plantlets grown under the LEDs resulted in taller plants with greater stem than fluorescent lamps. The shortest shoot length, 3.8 cm, was observed in the control and the longest one, 13.4 cm, in the red light. But the shoot length was 5.6 cm under red LED with supplemental blue(red+blue light). This results indicate that red LED may be suitable, in proper combination with other wavelengths of light. The root length under red light was significantly smaller among the treatments. The plantlets grown under red+blue light had lower shoot dry weight, higher dry matter than other lights-grown plantlets. Among the various growth parameters measered, there was an indication that leaf area was controlled by the LEDs. Leaf area of a plantlets developing under green light was about 2.4 times wider than that of plantlets grown under red LED (10.1 $\textrm{cm}^2$ in area). The dry matter rate per plantlet among the treatments was greater in plantlets grown under the red/blue LEDs in comparison with that grown under other LEDs. Chlorophyll contents in plantlets grown under the red, green, blue and red/blue LEDs were 2%, 7% 20% and 10% lower, respectively, than those in plant grown under fluorescent lamps.

  • PDF

Enhancement of flood stress tolerance for upland-adapted cereal crops by the close mixed-planting with rice

  • Iijima, Morio;Awala, Simon K;Hirooka, Yoshihiro;Yamane, Koji
    • Proceedings of the Korean Society of Crop Science Conference
    • /
    • 2017.06a
    • /
    • pp.44-44
    • /
    • 2017
  • Recently, the occurrences of extreme flooding and drought, often in the same areas, have increased due to climate change. We tested the hypothesis that wetland species could help upland species under flood conditions; that is, the roots of wetland crops may supply $O_2$ to the roots of upland crops by a series of experiments conducted in both humid Japan and semi-arid Namibia (See Iijima et al, 2016 and Awala et al, 2016). Firstly, flooding tolerance of upland-adapted staple crops-pearl millet (Pennisetum glaucum) and sorghum (Sorghum bicolor) mix-cropped with rice (Oryza spp.) was investigated in glasshouse and laboratory experiments in Japan. We found a phenomenon that strengthens the flood tolerance of upland crops when two species-one wetland and one drought tolerant-were grown using the mixed cropping technique that results in close tangling of their root systems, hereinafter referred to "close mixed-planting". This technique improved the photosynthetic and transpiration rates of the upland crops subjected to flood stress ($O_2$-deficient nutrient culture). Oxygen transfer was suggested between the two plants mix-cultured in water, implying its contribution to the phenomenon that improved the physiological status of upland crops under the simulated flood stress. Secondly, we further tested whether this phenomenon would be expressed under field flood conditions. The effects of close mixed-planting of pearl millet and sorghum with rice on their survival, growth and grain yields were evaluated under controlled field flooding in semi-arid Namibia during 2014/2015-2015/2016. Single-stand and mixed plant treatments were subjected to 11-22 day flood stress at the vegetative growth stage. Close Mixed-planting increased seedling survival rates in both pearl millet and sorghum. Grain yields of pearl millet and sorghum were reduced by flooding, in both the single-stand and mixed plant treatments, relative to the non-flooded upland yields, but the reduction was lower in the mixed plant treatments. In contrast, flooding increased rice yields. Both pearl millet-rice and sorghum-rice mixtures demonstrated higher land equivalent ratios, indicating a mixed planting advantage under flood conditions. These results indicate that mix-planting pearl millet or sorghum with rice could alleviate flood stress on dryland cereals. The results also suggest that with this cropping technique, rice could compensate for the dryland cereal yield losses due to field flooding. Mixed cropping of wet and dryland crops is a new concept to overcome flood stress under variable environmental conditions.

  • PDF

Adjustment Effect for Coping Strategy from Fatigue Scale of Security Guard's on Emotional Labor (시큐리티요원의 감정노동이 피로도에 미치는 영향 및 스트레스 대처방식의 조절효과)

  • Kim, Eui Young;Cho, Sung Jin
    • Korean Security Journal
    • /
    • no.60
    • /
    • pp.283-302
    • /
    • 2019
  • This study was conducted to provide the basic data for improving the working environment and welfare level of the security guard by identifying the primary effects of stress response on fatigue with emotional labor and identifying the adjustment effects with coping strategy. The data were collected from 214 security personnel using questionnaire and the statistical tests of correlation, heavy return analysis and hierarchical return analysis using the SPSS 18.0 statistics program were used to reach the conclusion that: First, it has been shown that the emotional labor of a security guard affects fatigue Second, the effects of emotional labor on fatigue of a security guard have been shown to have the effect of coping strategy. Based on the above studies, it was concluded that the emotional labor of a security guard was affected by fatigue and could be controlled by a stress coping strategy. Therefore, it is believed that there is a need to seek an autonomous corporate culture based on the organization's performance by changing the management system for employees at the organizational level so that security guards can use an active way of coping.

Minority report; Diketopiperazines and Pyocyanin as Quorum Sensing Signals in Pseudomonas aeruginosa (Minority report; Pseudomonas aeruginosa의 정족수 인식(쿼럼 센싱) 신호물질로써의 Diketopiperazines과 Pyocyanin)

  • Lee, Joon-Hee
    • Korean Journal of Microbiology
    • /
    • v.44 no.2
    • /
    • pp.85-92
    • /
    • 2008
  • Pseudomonas aeruginosa is an opportunistic human pathogen, causing a wide variety of infections including cystic fibrosis, microbial keratitis, and burn wound infections. The cell-to-cell signaling mechanism known as quorum sensing (QS) plays a key role in these infections and the QS systems of P. aeruginosa have been most intensively studied. While many literatures that introduce the QS systems of P. aeruginosa have mostly focused on two major acyl-homo serine lactone (acyl-HSL) QS signals, N-3-oxododecanoyl homoserine lactone (3OC12) and N-butanoyl homoserine lactone (C4), several new signal molecules have been discovered and suggested for their significant roles in signaling and virulence of P. aeruginosa. One of them is PQS (Pseudomonas quinolone signal; 2-heptyl-3-hydroxy-4-quinolone), which is now considered as a well-characterized major signal meolecule of P. aeruginosa. In addition, recent researches have also suggested some more putative signal molecules of P. aeruginosa, which are diketopiperazines (DKPs) and pyocyanin. DKPs are cyclic dipeptides and structurally diverse depending on what amino acids are involved in composition. Some DKPs from the culture supernatant of P. aeruginosa are suggested as new diffusible signal molecules, based on their ability to activate Vibrio fischeri LuxR biosensors that are previously considered specific for acyl-HSLs. Pyocyanin (1-hydroxy-5-methyl-phenazine), one of phenazine derivatives produced by P. aeruginosa is a characteristic blue-green pigment and redox-active compound. This has been recently suggested as a terminal signaling factor to upregulate some QS-controlled genes during stationary phase under the mediation of a transcription factor, SoxR. Here, details about these newly emerging signaling molecules of P. aeruginosa are discussed.

Favorable Irrigation Timing with Timer and Fruiting Position Focused on the Fruit Quality and Harvesting Time in Perlite Culture of Muskmelon (멜론의 펄라이트 재배시 타이머 제어에 의한 급액 시간과 착과절위가 과실의 품질과 수확시기에 미치는 영향)

  • Kim, H. J.;Kim, Y. S.
    • Journal of Bio-Environment Control
    • /
    • v.11 no.4
    • /
    • pp.157-162
    • /
    • 2002
  • The efficient timer-controlled irrigation and the favorable fruiting position were investigated far highly quality melon fruits from Feb.18 to July Si 1999. The nutrient solution was supplied either at every hour from 6:00 to 18:00 (T-1) or at 6:00, 8:00, 10:00, 11:00, 12:00, 12:30, 13:00, 13:30, 14:00, 14:30, IS:00,16:00, and 17:00 (T-2). A fruit was set at the first node of the fruit bearing branch from the 10, 12, or 13th node of the main stem. Pot weight was maintained at almost n constant level, regardless of the daily integrated solar radiation in T-2. Soluble solids content (SSC) and fresh weight of fruit were not significantly different among the irrigation treatments at each harvesting time. At the first harvest, SSC and fresh weight of fruit were not significantly different between the fruiting positions within the irrigation treatment. At the second harvest, SSC was higher in T-2 than T-1. The SSC was low in the fruit of the loth node in T-1, while it was not significantly different between fruiting positions in T-2. Fruit fresh weight was the highest at the 12 and 13th nodes in T-1, and the 13th node in T-2. Fresh and dry weights of leaf except petiole, regardless of harvesting time, increased as the node position was higher, The higher the fruiting position was, the lower the leaf weight was. Therefore, it is recommended to irrigate more frequently during the mid-noon. Fruits can be harvested earlier at the lower nodes in the spring crop production.

A Study on the Management of Foreign Crew in Domestic Merchant Vessel (내항상선 승선 외국인선원의 관리에 관한 연구)

  • Kim, Young-Mo
    • Journal of the Korean Society of Marine Environment & Safety
    • /
    • v.18 no.2
    • /
    • pp.123-129
    • /
    • 2012
  • Cabotage means transportation of cargoes or passengers between two points within same country by vessel or aircraft which registered to foreign country. Cabotage regulation has justified by the protectionism in economic aspect and by national security in the defence of a country, thus most world's major countries including United States of America, Japan and European Union(outside EU) are executing cabotage. Even though Korean's controlled fleet ranked to 5th in the world, shortage and ageing of Korean domestic seafarers came to the restricted factor in the Korean domestic shipping. Resultingly Korean government permitted 530 Myanmarese and Indonesian seafarers to come on board in domestic vessel. In regarding to the Korean law, there are no direct relationship between boarding foreign crew in domestic vessel. However the baxic concept of cabotage will be spoiled by boarding foreign crew under the regulation of cabotage, so special treatment should be needed to cover the problems in coastal shipping arose form them. To minimize the problems derived from foreign crew, following actions are recommended; first, management skills of foreign crew are needed to overcome differences of communication and culture; second foreign crew supporting center should be established to counsel their difficulties; third, high level of manning companies should be promoted; forth, stabilized crew supply should be guaranteed by improving employment condition; finally, memorandum of understanding should be concluded between two countries to prevent seceders.

Effect of PLGA/Silk Fibroin Hybrid Film on Attachment and Proliferation of Schwann Cells (실크피브로인을 함유한 PLGA 하이브리드 필름이 슈반세포의 부착과 증식에 미치는 영향)

  • Kim, Hye-Lin;Yoo, Han-Na;Park, Hyun-Jin;Kim, Yong-Gi;Lee, Dong-Won;Kang, Young-Sun;Khang, Gil-Son
    • Polymer(Korea)
    • /
    • v.35 no.1
    • /
    • pp.7-12
    • /
    • 2011
  • Poly(lactic-co-glycolic acid) (PLGA) is a biodegradable synthetic polymer with acceptable mechanical strength and well-controlled degradation rate. Also, it can be easily fabricated into many shapes. Silk fibroin contains powerful bioactive molecules. We fabricated natural/synthetic hybrid films using 0, 10, 20, 40 and 80 wt% of silk fibroin. Schwann cells (SCs) were seeded on PLGA/silk fibroin hybrid films and confirmed the effects of adhesion and proliferation on SCs according to the content of silk fibroin. In this study, we confirmed PLGA/silk fibroin hybrid film containing 40% and 80% of silk fibroin interrupted adhesion and proliferation of SCs. Films containing 10% and 20% of silk, however, provided suitable environment for growth and proliferation of SCs. These results suggest that silk fibroin provides suitables surface to neural cells and its proper content provides proper culture conditions to improve cell adhesion and proliferation.

Effect of a Soil Amendment for Controlling Fusarium Wilt of Cucumber caused by Fusarium oxysporum f. sp. cucumerinum (오이 덩굴쪼김병(Fusarium oxysporum f. sp. cucumerinum) 방제에 대한 토양첨가제의 효과)

  • Chung, Bong-Koo;Ryou, Na-Young
    • The Korean Journal of Mycology
    • /
    • v.24 no.2 s.77
    • /
    • pp.93-103
    • /
    • 1996
  • In order to find out effect on the inorganic and organic compounds as a soil amendment to control Fusarium wilt of cucumber caused by Fusarium oxysporum f. sp. cucumerinum, this study was conducted during the last two years from 1993 to 1995. According to add 14 inorganic chemicals (1%, w/v) including $Al_2(SO_4)_3$ individually in vitro, these chemicals were confirmed their suppression, and especially Alum, CaO and $Al_2(SO_4)_3$ suppressed not only $20.9{\sim}25.0$ percent on mycelial growth of the fungus, but also inhibited $72.8{\sim}97%$ on conidial germination. $Ca(NO_3)_2$ suppressed mycelial growth only, while KCl, $K_2SO_4,\;NH_4NO_3$, and Urea suppressed conidial germination. The 7 chemicals were finally seleced. Composted pine bark (CPB) suppressed definely more than 90% on conidial germination in the different extract concentration (2,5 and 10%), although mycelial growth on extract medium of CPB and milled alfalfa leaves (MAL) were not remarkable. The antagonist Trichoderma sp. (Tr-3) mixed with an amended soil (1%, w/w) containing composted pine bark showed a good mycelial growth to compete the causal fungus. And the antagonist Pseudomonas sp. (7-1-3) was also confirmed its antagonistic ability with culture filterate. It is known that a CPB soil amendment mixed with the two antagonists (1%, w/w) controlled almost completely Fusarium wilt of cucumber in greenhouse pots and a field experiment. It is therefore expected that biocontrol on Fusarium wilt of cucumber by a soil amendment can be applied to farmmer's fields.

  • PDF

Influence of Boron and Iron Toxicity on the Physiological Status, Growth, and Mineral Uptake of Ginseng in Hydroponic Culture (인삼 수경재배 시 붕소와 철 과잉 농도가 인삼의 생리장해 증상, 생육 및 무기원소 흡수에 미치는 영향)

  • Yu, Jin;Kang, Soo Hyun;Jang, In Bae;Jang, In Bok;Park, Ki Choon;Lee, Ueong Ho;Park, Hong Woo;Suh, Su Jeoung;Seo, Tae Cheol;Kim, Kee Hong
    • Korean Journal of Medicinal Crop Science
    • /
    • v.25 no.3
    • /
    • pp.175-182
    • /
    • 2017
  • Background: Ginseng is a perennial crop grown for more than four years in the same place. Therefore, it is highly affected by the soil environment, especially nutrients in the soil. The present study was carried out to investigate to the influence of boron and iron concentrations on the physiological status, growth, and mineral uptake of ginseng to obtain the basic information for diagnosing a physiological disorder in ginseng plants. Methods and Results: The boron and iron concentrations were controlled at 3, 30, 150, 300 and 2, 20, 100, $200mg/{\ell}$, respectively. When treated with $150mg/{\ell}$ of boron, the ginseng plants showed yellowing or necrosis symptoms at the edge or end of their leaves. Compared with the $3mg/{\ell}$ treatment, the root weight decreased by 13 and 24% in the 150 and $300mg/{\ell}$ treatments, respectively. When treated with $20mg/{\ell}$ of iron, the ginseng plants showed yellowing between the veins of the leaves followed by the formation of brown spots. The root weight gradually decreased with increasing iron concentration. Approximately 55% decrease in root weight was observed upon treatment with $200mg/{\ell}$ of iron. Conclusions: The boron toxicity occurs in the leaves of ginseng at the boron concentration of approximately 1,900 mg/kg or more. The iron toxicity occurs at the iron concentration of approximately 120 mg/kg for leaves and 270 mg/kg for roots.