• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.658-664
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• 2011

Rotifers of the genus Brachionus are commonly used as a live food for larval fish, and rotifers of different sizes are preferred according the mouth size of the fish. Rotifer species vary in size, and individual size can depend on the temperature and salinity of the rearing environment. We investigated the effects of temperature and salinity for two species, B. plicatilis (250-300 ${\mu}m$) and B. rotundiformis (100-220 ${\mu}m$). Two strains of B. plicatilis (CCUMP 36 and 48) and two strains of B. rotundiformis (CCUMP 51 and 56) were received from the Culture Collection of Useful Marine Plankton (CCUMP) at Pukyong National University and cultured with the green alga, Nannochloris oculata (KMMCC 16) from the Korea Marine Microalgal Culture Center (KMMCC). The growth and size of rotifers were examined at three water temperatures ($16^{\circ}C$, $24^{\circ}C$, $32^{\circ}C$) and four salinities (20 psu, 25 psu, 30 psu, 35 psu) under continuous light (40 ${\mu}molm^{-2}s^{-1}$). The maximum density and growth rate of B. rotundiformis were greater than those of B. plicatilis. The lorica length of B. plicatilis ranged from 215.4 to 269.7 ${\mu}m$ and from 154.9 to 206.6 ${\mu}m$ for B. rotundiformis, depending on strain, temperature and salinity. Rotifers were smaller when cultured at high temperatures, regardless of salinity. B. rotundiformis preferred higher salinity than B. plicatilis. The results demonstrated that the size of rotifers could be controlled to some extent by temperature and salinity.

• Journal of Microbiology
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• v.43 no.4
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• pp.375-380
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• 2005

Autonomous ultradian metabolic oscillation (T$\simeq$50 min) was detected in an aerobic chemostat culture of Saccharomyces cerevisiae. A pulse injection of GSH (a reduced form of glutathione) into the culture induced a perturbation in metabolic oscillation, with respiratory inhibition caused by $H_2S$ burst pro-duction. As the production of $H_2S$ in the culture was controlled by different amino acids, we attempted to characterize the effects of GSH on amino acid metabolism, particularly with regard to branched chain and sulfur-containing amino acids. During stable metabolic oscillation, concentrations of intra-cellular glutamate, aspartate, threonine, valine, leucine, isoleucine, and cysteine were observed to oscil-late with the same periods of dissolved $O_2$ oscillation, although the oscillation amplitudes and maximal phases were shown to differ. The methionine concentration was stably maintained at 0.05 mM. When GSH (100 $\mu$M) was injected into the culture, cellular levels of branched chain amino acids increased dramatically with continuous $H_2S$production, whereas the cysteine and methionine concentrations were noticeably reduced. These results indicate that GSH-dependent perturbation occurs as the result of the promotion of branched chain amino acid synthesis and an attenuation of cysteine and methionine synthesis, both of which activate the generation of $H_2S$. In a low sulfate medium containing 2.5 mM sulfate, the GSH injections did not result in perturbations of dissolved $O_2$ NAD(P)H redox oscillations without burst $H_2S$ production. This suggests that GSH-dependent perturbation is intimately linked with the metabolism of branched-chain amino acids and $H_2S$ generation, rather than with direct GSH-GSSG redox control.

• KSBB Journal
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• v.5 no.3
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• pp.307-313
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• 1990

A microcomputer-aided fermentation system was constructed for high density fed-batch culture using dissolved oxygen(DO) as a substrate feeding indicator. DO signal was processed prior to aquisition to computer. Agitation speed and oxygen flow rate was changed stepwisely to maintain DO value at a constant level. Agitation speed was controlled by the output signal of D/A converter. Oxygen flow rate was controlled by a flow rate control valve connected to a stepping motor. Substrate was fed with a feeding pump operated by the abrupt increase of DO signal. Methylobacillus sp. SK1 was cultivated to test the system and 16.53g/l of cell density was obtained after 10 hr.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.348-354
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• 1996

This study was carried out to investigate factors influencing on the groundwater quality at controlled horticulture in Honam area in 1995. The deeper groundwater sampling, the lower concentrations of $NH_4-N$ and $NO_3-N$ were observed. There was no difference in concentrations of $SO_4\;^{2-}$ and $Na^+$ in the groundwater below 15m. Contents of $NH_4-N$, $NO_3-N$ and $PO_4\;^{3-}$ in groundwater were the highest at rice transplanting season(the late May) and that of $SO_4\;^{2-}$, $Na^+$ and $Cl^-$ were the highest at dry season(the mid-February). Continuous cultivation of horticultural crops showed higher concentration of $NH_4-N$, $NO_3-N$, $PO_4\;^{3-}$, $Na^+$ and $Cl^-$ in groundwater than rotational culture with rice. The longer cultivation years with horticultural crops, the higher concentrations of $NO_3-N$, $PO_4\;^{3-}$, $SO_4\;^{2-}$ and $Cl^-$ were shown, and constitutional ratio of $NO_3-N$ among the anions increased gradually. Nitrate-N level, exceeded 20mg/l, the critical level for agricultural usage, frequently at Yongjinmyeon Wanju and Janglockdong Kwangju, and $PO_4\;^{3-}$ levels were higher at Seogtandong Iksan than the other places.

• Journal of Plant Biology
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• v.22 no.1_2
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• pp.35-43
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• 1979

The present investigation has been made to study the deficiency symptoms of boron on the formation of cell wall and the development of the individual components of the orchid cell wall. Analytical samples were taken from two sources; one from the individual orchid plants started from an apical meristem culture followed by the generation of the protocorm-like body which was developed into a plant, the other from the plant cultivated in water for 30 days. The amount of boron in the cultrues were controlled and the deficiency symptoms were observed under theelectron microscope, optical microscope with samples taken from the zones of elongation of leaves and compared the dry weight of cell walls and finally the various fractions of the cell wall components. The following results were obtained: (1) The growth of roots and leaves was hampered in the boron deficient plants. (2) In the boron-deficient leaves a severe necrosis and cracks were developed in the tissue of zone of elongation besides the decrease in growth. (3) under the electorn microscope the cell walls of boron-deficient plants showed rough undulated structures unlike the smooth control cell walls. (4) the dry weight of total cells and cell walls of boron deficient plants were higher than the control plants. (5) In the boron deficient plant the amout of pectin and hemicellulose isolated from cell walls were higher and the amount of protein was lower than the controlled plots.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.90.2-90.2
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• 2013

Vertically-aligned silicon nanostructure arrays (SNAs) have been drawing much attention due to their useful electrical properties, large surface area, and quantum confinement effect. SNAs are typically fabricated by chemical vapor deposition, reactive ion etching, or wet chemical etching. Recently, metal-assisted chemical etching process, which is relatively simple and cost-effective, in combination with nanosphere lithography was recently demonstrated for vertical SNA fabrication with controlled SNA diameters, lengths, and densities. However, this method exhibits limitations in terms of large-area preparation of unperiodic nanostructures and SNA geometry tuning independent of inter-structure separation. In this work, we introduced the layerby- layer deposition of polyelectrolytes for holding uniformly dispersed polystyrene beads as mask and demonstrated the fabrication of well-dispersed vertical SNAs with controlled geometric parameters on large substrates. Additionally, we present a new means of building in vitro neuronal networks using vertical nanowire arrays. Primary culture of rat hippocampal neurons were deposited on the bare and conducting polymer-coated SNAs and maintained for several weeks while their viability remains for several weeks. Combined with the recently-developed transfection method via nanowire internalization, the patterned vertical nanostructures will contribute to understanding how synaptic connectivity and site-specific perturbation will affect global neuronal network function in an extant in vitro neuronal circuit.

• Journal of English Language & Literature
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• v.58 no.4
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• pp.589-609
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• 2012

This research compares a future society described in Ray Bradbury's Fahrenheit 451 (1953) to modern technopoly. The main protagonist of the novel, Guy Montag, is a fireman who burns books in a future society which does not allow people to read or own books. The future society which controls the expansion of knowledge is similar to technopoly which Neil Postman defines as a culture where people passively react to overflow of information. Bradbury compares Montag to several characters, such as his wife Mildred and Captain Beatty. With this comparison, Bradbury lets his readers look back themselves who live in a sea of information without being aware of the domination of technopoly. This research suggests that the reason people do not know that knowledge is controlled and limited is because they do not distinguish between knowledge and information. They misunderstand widely available information is knowledge as characters in Fahrenheit 451 feel stuffed with information. Since the 1990s, scholars and writers such as Neil Postman and Nicholas Carr have expressed problems with the excess of information, however Bradbury already predicted through Fahrenheit 451 in 1953 that the development of technology does not mean a higher level of knowledge. This research suggests what modern human beings have lost in vast amount of information rather than what they have gained.

• SUVANNABHUMI
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• v.5 no.2
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• pp.67-80
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• 2013

A widely shared view identifies civil society with the set of nonprofit or nongovernmental organizations. This tradition reflects a long history of social theory viewed in institutional terms. Myanmar society has developed, mainly based on Buddhist culture and tradition. Buddhist practice necessitates public validation within an economy of merit in which moral action earns future rewards. Myanmar private voluntary associations have, since ancient times, played a vital role in achieving social purposes. Buddhist monasteries were the main education institutions in Myanmar. Until today, monastic schools, or monastic education centers as they are often called, have been the most important civil-society institutions bridging the accessibility gap in the state-run education system in government-controlled areas. Apart from social or community based that serve for public health in Myanmar there has been a number of religious organizations serving for the same. Moreover there appeared an association to very differently help those who need much urgent help. Since military coup in 1962, however, successive regimes have sought to stamp out civil society and permit only state-controlled organizations. Civil society re-emerged during the nation-wide pro-democracy demonstrations in 1988. After 1988 civil societies are allowed as like a political party to form. Now a day some civil societies have worked to start small-scale projects addressing local problems, but they must stay clear of politics.

• Korean Journal of Social Welfare
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• v.63 no.2
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• pp.81-107
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• 2011

The purpose of the study is to examine the effect of error management culture and psychological capital on job satisfaction and organizational commitment from social workers. This study was focused on the relationship between error management culture, psychological capital and job satisfaction and organizational commitment, controlled organizational fairness. The data was collected from social workers in Seoul, Kyunggi areas using stratified sampling method. A total of 564 social workers and 89 human service organizations were finally used for multilevel analysis. The survey had conducted for 18 days, from October 27 to November 13 in 2009 by mail. The major finding of this study are as follows. First, interaction effect between error management culture and psychological capital was significant job satisfaction and organizational commitment in positive direction. Second, main effect of error management culture in human service organizations was positively significant job satisfaction and organizational commitment on social workers. So it was important to enhance the level of error management culture in order to increase the job satisfaction and organizational commitment. Third, main effect of psychological capital on social workers was positively significant job satisfaction and organizational commitment. According to the results of this study, it was suggested the theoretical and practical implications for increasing and strengthen the error management culture and psychological capital.

• KSBB Journal
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• v.14 no.3
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• pp.284-290
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• 1999

Glucose fed-batch culture was studied to improve cellulose productivity by Acetobacter xylinum BRC5. When initial glucose concentrations in batch cultures were less than 20 g/L, yield coefficients of cellulose (Yp/s) remained a constant value of 0.21 g cellulose/g glucose. But a low yield coefficient, Yp/s=0.13 was obtained from an initial glucose concentration of 40 g/L. Since initial high glucose concentrations in batch culture resulted in low yields of cellulose, constant fed-batch cultures were carried out. The optimal feed rate for fed-batch culture was 2.22 g glucose/L.h. In constant fed-batch culture without DO control, 10 g/L of cellulose was obtained from 40 g/L of glucose with this feed rate, which was approximately two fold higher than that of the batch culture with the same initial glucose concentration. In DO stat plus fed-batch culture, the highest cellulose productivity could be obtained when dissolved oxygen level was controlled at 10% of air saturation, and cellulose productivity increased about 1.5 times compared with that of the culture without DO control.