• Clinical and Experimental Reproductive Medicine
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• 제23권1호
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• pp.87-94
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• 1996

For evaluating the suitability of human follicular fluid(HFF) as protein supplement in ART, this preliminary study was performed to examine the maturation promoting activity of HFF on mouse follicular oocytes in vitro. Mouse follicular oocytes were collected from ovaries of 21-28 day old ICR mice by puncturing the antral follicles with fine needle at 48 hours after PMSG injection. The oocytes were rinsed and cultured in modified Whittingham's $T_6$ medium containing purines or dbcAMP to maintain meiotic arrest, and different concentrations of HFF were added into the culture medium to examine the effect of HFF on releasing the oocytes from the suppressive influence of the meiotic inhibitors. As a control for HFF, the maturation promoting activity of human fetal cord serum(HFCS) was investigated and compared with the activity of HFF. While HFF was separated, by molecular weight(M.W), into high M.W. fraction(M.W>30,000) and low M.W. fraction(M.W<30,000) and the effects of the fractions on meiotic resumption were investigated in the presence of the meiotic inhibitors. Also hormone analysis was performed to compare the content of hormones in HFF with that in HFCS. Same concentrations of HFF and HFCS induced similar germinal vesicle break down(GVBD) rates of the oocytes meiotic arrested by purines(4mM hypoxanthine+0.75mM adenosine), but the extrusion rate of 1st polar body(PB) of the oocytes cultured in HFF(65.0%, P<0.05) was significantly higher than that(51.6%) in HFCS. While, in the presence of 200 M dbcAMP, the maturation promoting activity of HFF (GVBD: 70.5%, $p<10^{-6}$; 1st PB extrusion: 67.1%, $p<10^{-3}$) was significantly greater than that of HFCS(GVBD: 35.2%; 1st PB extrusion: 41.1%). The oocytes cultured in the fraction of HFF containing high M.W. components showed higher meiotic maturation rates than the oocytes cultured in the low M.W. fraction of HFF. Gonadotropins and $E_2$ were known to improve the completion of maturation changes, and the levels of these hormones were higher in HFF than in HFCS. Therefore, HFF was more effective than HFCS to use for promoting meiotic resumption of mouse oocytes in vitro.

• Journal of Trauma and Injury
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• 제33권1호
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• pp.13-17
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• 2020

Purpose: Accurate and appropriate prehospital field triage is essential for a trauma system. The Korean trauma system (established in 2014) uses the trauma field triage algorithm of the United States Centers for Disease Control and Prevention (CDC). This study evaluated the suitability of the CDC field triage criteria for major trauma cases (injury severity score >15) in Korea. Methods: This retrospective cohort study evaluated trauma patients who presented at the authors' regional trauma center from January 1 to May 7, 2017. The undertriage and overtriage rates of each CDC field triage step were calculated. Receiver operating characteristic curves were constructed, and the area under the curve (AUC) was evaluated for each step. Results: Among the 1,009 enrolled patients, 168 (16.7%) had major trauma. The undertriage/overtriage rates of each step (steps I, II, III, and IV) of CDC field triage were 9.2%/47.4%, 6.3%/50.8%, 4.5%/59.4%, and 5.3%/78.9%, respectively. The AUC values of each CDC triage step were 0.722, 0.783, 0.791, and 0.615, respectively. The AUC values of the separate components of each step (physiologic criteria, anatomic criteria, mechanism-of-injury criteria, and special considerations) were 0.722, 0.648, 0.647, and 0.456, respectively. Conclusions: The CDC field triage system is acceptable, but not ideal, for Korean trauma care. If we follow the protocol, it would be preferable to omit step IV. The Korean Triage and Acuity Scale may be a good indicator for in-hospital triage. However, a new triage protocol that is simple to estimate on-scene while having good performance should be developed.

• 기록학연구
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• 제14호
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• pp.91-117
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• 2006

전자기록은 평가 시 가치 평가와 진본 여부 평가를 함께 수행해야 한다. 그간 기록의 가치 평가에 대해서는 여러 논의가 진행되어 왔지만 진본 평가에 대해서는 그러하지 못했다. 이 글에서는 진본 평가가 기록관리 각 과정의 어느 국면에서 필요하고 그 구체적인 방법은 무엇인가를 밝히고 있다. 대체로 입수단계에서는, 생산기관에서의 재생산 직후의 일치 검증, 수신된 전자기록의 품질 및 일치 검증, 입수기록패키지와 보존기록패키지의 일치 검증이 필요하다. 저장단계에서는 매체수록된 보존기록패키지의 일치 검증, 저장된 전자기록의 손상여부 검사와 복구가, 각종 처리의 단계에서는 관리기준값 변경 처리 후의 적절성 평가, 기록철 분류구조 변경 후의 평가, 마이그레이션 이후의 일치평가 및 기타 주기적 무결성 평가, 배부기록의 일치 평가 등이 요구된다. 이러한 진본 평가를 위해서는 일치검증평가, 내용적 동일성 확인 평가, 메타데이터 요소의 적절성 평가, 불법적 변경여부의 확인 평가, 물리적 상태평가 등의 방법이 적용되어야 한다.

• 생물환경조절학회지
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• 제25권2호
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• pp.100-105
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• 2016

양파 정식기 작업에 적합한 상토 개발을 위해 원예용 유기질 주재료(피트모스, 코이어)의 적합성을 조사했다. 유기질 재료를 무기질 재료(황토 등)와 혼합하여, 묘 출현율, 생육 및 기계정식 적응 특성 등을 조사하였다. 조사 결과 피트모스 혼합 처리구, 코이어 혼합 처리구 모두 묘 출현율, 생육 및 상토 용적밀도에서 유사했으나, 피트모스 혼합 처리구가 코이어 혼합 처리구에 비해 더 높은 상토 응집성을 보였다. 피트모스 혼합 상토의 높은 응집성은 양파 묘 정식 시 뿌리부 무게 증가 등의 효과를 가져와서 정식기 작업 효율성을 증진시키는데 도움을 줄 수 있을 것이라고 생각된다. 이러한 이유로, 양파 정식기 작업에 적합한 상토 개발 시 피트모스를 유기질 주재료로 선정하는 것이 적합하다고 최종 판단하였다.

• 한국산학기술학회논문지
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• 제11권10호
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• pp.3859-3865
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• 2010

신호를 생성할 수 있는 상용 신호 생성 장치 그리고 데이터 수집 장비와 DSP 보드를 이용하여 가상의 리더와 태그를 모델링 한 에뮬레이터 시스템을 구현했다. 개발한 가상의 UHF RFID(860~960[MHz]) 리더/태그 모듈을 이용하여 개발한 RFID 리더와 태그의 프로토콜 및 RF 특성 등이 국제 표준 규격(ISO 18000-6 Type C, EPCglobal C1G2)에 적합 여부를 검증할 수 있는 방법을 제공해 줄 수 있게 하였다. 본 논문에서 제안한 가상의 리더 모델과 태그 모델을 구현하기 위해 DSP 보드를 이용하여 Visual DSP로 구현하였으며, 시스템의 신호발생기, 신호 분석기, 성능 검증 대상 리더 또는 태그, RFID 에뮬레이터 제어 컴퓨터 및 제어 프로그램으로 구성하였다.

• 한국항공우주학회지
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• 제40권11호
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• pp.934-939
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• 2012

본 연구는 무인항공기의 환경제어계통에 적용되는 PAO-공기 열교환기에 대한 성능검증에 관한 것으로, 계통에 대한 성능분석을 통해 열교환기 성능목표를 설정하고 상세설계와 정밀제작을 통해 목표성능에 부합되는 열교환기를 개발하였다. 개발된 PAO-공기 열교환기의 성능을 입증하기 위해 열교환기의 설계점 외에 다양한 시험조건에 대한 압력손실과 열전달 유용도, 총괄열전달계수 등에 대해 시험을 수행하였다. 수행된 결과를 바탕으로, 무인항공기용 환경제어계통에 적용된 PAO-공기 열교환기의 성능과 특성을 분석하여 개발적합성에 대해 검증하였다.

• International Journal of Oral Biology
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• 제35권1호
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• pp.1-5
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• 2010

Human fibroblasts that maintain the structural integrity of connective tissues by secreting precursors of the extracellular matrix are typically cultured with serum. However, there are potential disadvantages of the use of serum including unnatural interactions between the cells and the potential for exposure to animal pathogens. To prevent the possible influence of serum on fibroblast cultures, we devised a serum-free growth method and present in vitro data that demonstrate its suitability for growing porcine fetal fibroblasts. These cells were grown under four different culture conditions: no serum (negative control), 10% fetal bovine serum (FBS, positive control), 10% knockout serum replacement (KSR) and 20% KSR in the medium. The proliferation rates and viabilities of the cells were investigated by counting the number of cells and trypan blue staining, respectively. The 10% FBS group showed the largest increase in the total number of cells ($1.09\;{\times}\;10^5\;cells/ml$). In terms of the rate of viable cells, the results from the KSR supplementation groups (20% KSR:64.7%; 10% KSR: 80.6%) were similar to those from the 10% FBS group (68.5%). Moreover, supplementation with either 10% ($3.0\;{\times}\;10^4\;cells/ml$) or 20% KSR ($4.8\;{\times}\;10^4\;cells/ml$) produced similar cell growth rates. In conclusion, although KSR supplementation produces a lower cell proliferation rate than FBS, this growth condition is more effective for obtaining an appropriate number of viable porcine fetal fibroblasts in culture. Using KSR in fibroblast culture medium is thus a viable alternative to FBS.

• 복식
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• 제62권7호
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• pp.205-219
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• 2012

The purpose of this study is to develop comfortable patient gown for spinal surgery patients. The results of the survey confirmed inconveniences and problems with the current gown, and a new gown style was recommended. The recommended changes addressed design, pattern, and materials. The final experimental design for the top of the new patient gown is a wrap style that moves the center opening of the gown to the side and enables patients to control the length of the sleeves by attaching two snaps. The cutting line is aligned with the back brace location, and the issue of repeated bunching of the gown material by the brace was solved by substituting 100% knitted structure fiber. The sleeve length can be adjusted with snaps on the sleeve cap and hem. When lifting up the gown for treatment on specific areas of the body, doctors open the edge of the right side of the gown in order to lift up the top. The bottom of the new patient gown was made from 100% cotton knitted structure fiber, and it enables patients to control the length of pants by attaching two snaps on the side. The results of an on-site dressing suitability evaluation and a flexibility evaluation with respect to dressing/undressing indicated that the new patient gown was much better received than the existing gowns (A and B). Our hypothesis regarding new patient gowns was supported in both the fit and design categories.

• 한국화재소방학회논문지
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• 제24권2호
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• pp.58-66
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• 2010

정부의 다양한 노력에도 불구하고 재난으로 인한 피해가 지속적으로 증가함에 따라 효율적이고, 과학적이며, 체계적인 재난관리체제의 강화를 위해서는 최근에 급속히 진보되고 있는 유비쿼터스 정보기술의 활용이 필수적이라 할 수 있다. 2008년 지령관제 GPS시스템과 유비쿼터스 119 신고시스템이 도입되어 시행되고 있지만 그 효과성은 크게 나타나지 않고 있다. 본 연구에서는 유비쿼터스 119 신고시스템의 실태 분석과 선행연구, 실증분석 등을 통해 효과적인 재난관리 방안을 제안하는데 목적을 두고 있다. 연구결과 활용도, 적합성, 정보획득 용이성, 사용의향은 높은 인식을 하고 있는 것으로 나타나 재난관리 분야에 UIT를 확대 적용하여도 큰 문제점이 없을 것이다. 지령관제 GPS시스템은 정보 전달에 있어 신속성이 확보된다면 큰 효과를 기대할 수 있다.

• 약학회지
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• 제45권2호
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• pp.227-236
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• 2001

The complexity and variability of both the biologicals and the bioassays used to test them led to the use of the reference standard- a sample of the product of defined purity and potency, against which all preparations of that product must be calibrated. In order to prepare and establish KFDA reference standard for recombinant human growth hormone (somatropin), somatropin substance was filled in ampoules in National Institute for Biological Standards and Control (NIBSC). The candidate KFDA reference standard for somatropin (designated as 98/674) was evaluated to determine the suitability of serving as a KFDA reference standard for somatropin by the collaborative study, in which 10 laboratories participated. Physicochemical analysis and in vivo bioassay were performed by direct comparison with the international somatropin standard 88/624. 98/674 was identified as somatropin by SDS-PAGE, IEF, peptide mapping, and HPLC. Determination of somatropin content by SE-HPLC yielded a mean estimate of 2.01 mg somatropin per ampoule. Data from the study also yielded mean values of 0.39 $\pm$ 0.26% for high molecular weight impurities by SE-HPLC and mean values of 2.13 $\pm$ 1.29% for somatropin related proteins by RP-HPLC. Estimates of relative potency by weight gain bioassay in the hypophysectomised rats showed that relative potency of KS 98/674 was 1.07 aganist IS 88/624. Based on the results of the collaborative study, the candidate reference standard for somatropin is suitable to serve as a KFDA reference standard for somatropin.