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Biological Control of Apple Anthracnose by Paenibacillus polymyxa APEC128, an Antagonistic Rhizobacterium

  • Kim, Young Soo;Balaraju, Kotnala;Jeon, Yongho
    • The Plant Pathology Journal
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    • v.32 no.3
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    • pp.251-259
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    • 2016
  • The present study investigated the suppression of the disease development of anthracnose caused by Colletotrichum gloeosporioides and C. acutatum in harvested apples using an antagonistic rhizobacterium Paenibacillus polymyxa APEC128 (APEC128). Out of 30 bacterial isolates from apple rhizosphere screened for antagonistic activity, the most effective strain was APEC128 as inferred from the size of the inhibition zone. This strain showed a greater growth in brain-heart infusion (BHI) broth compared to other growth media. There was a reduction in anthracnose symptoms caused by the two fungal pathogens in harvested apples after their treatment with APEC128 in comparison with non-treated control. This effect is explained by the increased production of protease and amylase by APEC128, which might have inhibited mycelial growth. In apples treated with different APEC128 suspensions, the disease caused by C. gloeosporioides and C. acutatum was greatly suppressed (by 83.6% and 79%, respectively) in treatments with the concentration of $1{\times}10^8$ colony forming units (cfu)/ml compared to other lower dosages, suggesting that the suppression of anthracnose development on harvested apples is dose-dependent. These results indicated that APEC128 is one of the promising agents in the biocontrol of apple anthracnose, which might help to increase the shelf-life of apple fruit during the post-harvest period.

Effects of Streptomyces griseofuscus 200401 on Melon Powdery Mildew in Greenhouse

  • Lim, Tae-Heon;Cho, Jeong-Sang;Kang, Sang-Jae;Johnson, Iruthayasamy;Cha, Byeong-Jin;Choi, Yong-Hwa
    • The Plant Pathology Journal
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    • v.24 no.1
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    • pp.63-66
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    • 2008
  • The fermentation broth (FB) of Streptomyces griseofuscus 200401 isolated from non-farming soil showed antagonistic activity against powdery mildew fungus both in melon leaf/seedling assay and in field trials. The FB of S. griseofuscus 200401 was tested at different concentrations. In primary test, the control value of 2-fold diluted FB of S. griseofuscus 200401, compared to control, reached to 82.8%. The protective activity recorded 80.5% in 2-fold dilution of FB. The effect was reduced to 28.9% in high dilution (100-fold) treatment. The curative effect was relatively lower than protective activity. In field test, the antifungal activities of S. griseofuscus 200401 remained low in blocks sprayed with 100-fold diluted FB and the control values were 15.2 and 15.9% in 2005 and 2006, respectively. However, the activities were as high as 65% and 67.3% in the blocks treated with 2-fold dilution of FB during the same period.

Effect of aquatic exercise on balance and depression of stroke patients (수중운동이 뇌졸중 환자의 균형과 우울에 미치는 효과)

  • Kim, Se-Hun;Lee, Dong-Kyu;Kim, Eun-Kyung
    • The Journal of Korean Physical Therapy
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    • v.26 no.2
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    • pp.104-109
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    • 2014
  • Purpose: The purpose of this study was to understand the effect of aquatic exercise on balance and depression of stroke patients. Methods: For 12 participants with stroke, six members of the experimental group and six members of the control group were randomly selected and arranged. Proprioceptive Neuromuscular Facilitation (PNF) lower extremity aquatic pattern exercise was applied to the experimental group. On the other hand, participants in the control group performed PNF lower extremity pattern exercise on the ground. Both exercises were performed 30 minutes per day, five times per week, for a period of six weeks. Balance was assessed using the Berg Balance Scale (BBS) and Timed Up to Go test (TUG). Depression was measured using the Center for Epidemiologic Studies depression scale (CES-D). Results: As a result of comparison within groups, the experimental group showed significant difference for balance and depression after the experiment (p<0.05). In comparison between the two groups, the experimental group in which aquatic exercise was applied showed more significant change in balance and depression than the control group (p<0.05). Conclusion: According to the results of this study, aquatic exercise was proven to enhance the balance and decrease the depression of stroke patients.

Effects of EMG-biofeedback Training on Total Knee Replacement Patients' Lower Extremity Muscle Activity and Balance (근전도-생체되먹임 훈련이 무릎관절 전치환술 환자의 하지 근활성도와 균형에 미치는 영향)

  • Park, Seung-Kyu;Kim, Je-Ho
    • The Journal of Korean Physical Therapy
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    • v.25 no.2
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    • pp.81-87
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    • 2013
  • Purpose: The purpose of the current study was to examine the effects of electromyography (EMG)-biofeedback training on lower extremity muscle activity and balance of patients with total knee replacement (TKR). Methods: Subjects were randomly allocated to two groups: experimental and control group. Subjects in the experimental group (n=10) were provided with quadriceps setting exercise by EMG-biofeedback (QSE+BF) and those in the control group were provided with QSE. Subjects in both groups were provided with the respective training programs for 20 minutes per session, five times per week, for a period of six weeks. To test significance, data analysis was performed using repeated-ANOVAs. Results: Statistically significant differences in muscle activity of the rectus femoris muscle and the vastus lateralis, and dynamic balance ability were observed in the experimental group, compared with the control group. In comparison of the muscle activity of the rectus femoris muscle and the vastus lateralis, and dynamic balance ability between different training periods within the groups, both groups showed statistically significant differences. Conclusion: EMG-biofeedback training is effective in improving lower extremity muscle activity and balance ability of patients with TKR, and should be effective in patients with other diseases.

A Study on the Quality Characteristics of Doenjang Prepared with Paecilomyces japonica, from Silkworm (누에 동충하초(Paecilomyces japonica)를 첨가하여 제조한 된장 품질특성 변화에 관한 연구)

  • 방혜열;김건희
    • Korean journal of food and cookery science
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    • v.19 no.6
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    • pp.694-700
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    • 2003
  • Changes in the Qualify Properties of Doenjang Prepared using the Powder or extracts of Paecilomyces japonica were investigated during fermentation at 20$^{\circ}C$. The moisture content was increased during fermentation but was not significantly different in all treatments. The pH of Doenjang with p. japonica was lower than the control group and decreased with the fermentation time. The amino nitrogen content increased gradually for up to 60 days and decreased slightly at 90 days. The L, a and b value decreased in proportion to the fermentation period and those of Doenjang with P. japonica powder were the lowest. From the results of the sensory evaluation, the color of the control group was similar to "yellow" but that of the Doenjang made from powder of P. japonica was close to "dark brown" and those of the Doenjang made from the P. japonica extract were darker than that of the control group. The texture was "glossy and smooth" in all cases and preference about the texture was high. The Doenjang with added P. japonica Powder had a saltier taste and the Doenjang with P. japonica Powder had the least sweet taste. In the flavor and overall Preference, the Doenjang with P. japonica powder was the lowest.

Development and Validation of a Postpartum Care Mobile Application for First-time Mothers (초산모를 위한 산후관리 모바일 앱 개발 및 효과 검증)

  • Lee, Ju Yeon;Kim, Hye Young
    • Women's Health Nursing
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    • v.23 no.3
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    • pp.210-220
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    • 2017
  • Purpose: The aims of the study were to develop mobile application for postpartum care of first-time mothers and to validate it's effect. Methods: Using a nonequivalent control group pretest-posttest design, 52 first-time mothers were recruited (26 each in experimental and control) and the experimental group used the mobile application for 6 weeks after delivery. Postpartum self-care knowledge and confidence, infant care knowledge and confidence, and postpartum depressive mood (Edinburgh Postnatal Depression Scale) were measured before discharge from the hospital and 6 weeks later. Results: Women who have used the postpartum care mobile application reported higher levels of postpartum self-care knowledge (p=.030) and confidence (p=.023) infant care knowledge (p=.001) and confidence (p=.004), while scores of postpartum depression (p=.021) were lower than those in the control group. Conclusion: The postpartum-care mobile application developed in this research may be effective in reinforcing knowledge and confidence for postpartum self-care and infant care and in reducing postpartum depressive mood.

Efficacy of Mannatide Combined with Sodium Cantharidate Vitamin B6 in the Treatment of Malignant Pleural Effusions

  • Wang, Li-Zhi;Zhang, Hong-Juan;Song, Ji
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.9
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    • pp.3913-3916
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    • 2015
  • Objective: To evaluate the efficacy of mannatide combined with sodium cantharidate vitamin B6 in the treatment of malignant pleural effusions. Materials and Methods: Data for 69 patients with malignant pleural effusions who did not receive systemic chemotherapy were collected. Injection into the thorax using mannatide combined with sodium cantharidate vitamin B6 was performed for 37 patients in the experimental group and mannatide combined with cisplatin for 32 patients in the control group. Objective responses, KPS (Karnofsky Scoring) and incidences of side effects between the two groups were compared. Results: 13 patients reached CR (complete response) and 11 PR (partial response) in the experimental group, while 12 patients reached CR and 9 PR in the control group, the difference in overall objective responses between the two groups not being significant (66.7% vs 63.6%, p=0.806). However, improvement of KPS in the experimental group wasgreater than in the control group; total side-effect incidences during the period of treatment were 22.2% (8/36) and 54.5% (18/33), respectively (p=0.006). Conclusions: Regimen of mannatide combined with sodium cantharidate vitamin B6 had better improvement in quality-of-life and symptom relief, with a lower side-effect incidence in treatment of malignant pleural effusions.

New Ignition Method and Ignition Recognition Logic for a Microturbine (마이크로터빈의 새로운 점화 기법과 점화 인식 로직 개발)

  • Kim, Gi-Rae;Choi, Young-Kyu;Rho, Min-Sik
    • Journal of Institute of Control, Robotics and Systems
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    • v.13 no.2
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    • pp.179-186
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    • 2007
  • This paper presents new ignition method and ignition recognition logic for a microturbine. New ignition method is designed by constant speed control of a microturbine with pre-determined time during a ignition period. It make more accurate air-fuel ratio as well as give enough time to ignition system to have full performance under cold temperature. And ignition recognition logic is designed by observing output current change of inverter by generating output torque of a microturbine in the instant of ignition. For filtering a output torque current of inverter with high frequency, we applied a moving average method. So far, ignition recognition is usually implemented by measuring of exhausted gas temperature(EGT) of microturbine. The proposed logic can give more accurate judgement of ignition as well as keep a good working of starting system under out of order a temperature measuring system and biased initial value of EGT sensor. Finally, the two proposed logics are proved by field operating a microturbine under various conditions.

Effects of Repetitive Transcranial Magnetic Stimulation on Motor Recovery in Lower Extremities of Subacute Stage Incomplete Spinal Cord Injury Patients: A Randomized Controlled Trial

  • Ji, Sang-Goo;Cha, Hyun-Gyu;Kim, Myoung-Kwon
    • Journal of Magnetics
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    • v.20 no.4
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    • pp.427-431
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    • 2015
  • The aim of this study was to investigate whether repetitive transcranial magnetic stimulation (rTMS) can improve motor recovery in the lower extremities of the patients with subacute stage spinal cord injury (SCI). This study was conducted with 19 subjects diagnosed with paraplegia because of SCI. The experimental group included 10 subjects who underwent active rTMS, and the control group included 9 subjects who underwent sham rTMS. The SCI patients in the experimental group underwent conventional rehabilitation therapy, and active rTMS was applied daily to the hotspot of the lesional hemisphere. The SCI patients in the control group underwent sham rTMS and conventional rehabilitation therapy. The participants in both the groups received therapy five days per week for six weeks. Latency, amplitude, and velocity were assessed before and after the six-week therapy period. A significant difference in post-treatment gains for the latency and velocity was observed between the experimental and control groups (p < 0.05). However, no significant differences in the amplitude were observed between the two groups (p > 0.05). The results of this study indicate that rTMS may be beneficial in improving motor recovery in the lower extremities of subacute stage SCI patients.

Parallel Load Techinques Application for Transcranial Magnetic Stimulation

  • Choi, Sun-Seob;Kim, Whi-Young
    • Journal of Magnetics
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    • v.17 no.1
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    • pp.27-32
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    • 2012
  • Transcranial magnetic stimulation requires an electric field composed of dozens of V/m to achieve stimulation. The stimulation system is composed of a stimulation coil to form the electric field by charging and discharging a capacitor in order to save energy, thus requiring high-pressure kV. In particular, it is charged and discharged in capacitor to discharge through stimulation coil within a short period of time (hundreds of seconds) to generate current of numerous kA. A pulse-type magnetic field is formed, and eddy currents within the human body are triggered to achieve stimulation. Numerous pulse forms must be generated to initiate eddy currents for stimulating nerves. This study achieved high internal pressure, a high number of repetitions, and rapid switching of elements, and it implemented numerous control techniques via introduction of the half-bridge parallel load method. In addition it applied a quick, accurate, high-efficiency charge/discharge method for transcranial magnetic stimulation to substitute an inexpensive, readily available, commercial frequency condenser for a previously used, expensive, high-frequency condenser. Furthermore, the pulse repetition rate was altered to control energy density, and grafts compact, one-chip processor with simulation to stably control circuit motion and conduct research on motion and output characteristics.