• Fisheries and Aquatic Sciences
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• v.20 no.8
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• pp.16.1-16.8
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• 2017

The exportation of cultured olive flounder (Paralichthys olivaceus) in Korea has been recently decreasing due to the infections with a myxozoan parasite Kudoa septempunctata, and there is a strong demand for strict food safety management because the food poisoning associated with consumption of raw olive flounder harbouring K. septempunctata has been frequently reported in Japan. The life cycle and infection dynamics of K. septempunctata in aquatic environment are currently unknown, which hamper establishment of effective control methods. We investigated sea water and marine invertebrates collected from olive flounder farms for detecting K. septempunctata by DNA-based analysis, to elucidate infection dynamics of K. septempunctata in aquaculture farms. In addition, live marine polychaetes were collected and maintained in well plates to find any possible actinosporean state of K. septempunctata. The level of K. septempunctata DNA in rearing water fluctuated during the sampling period but the DNA was not detected in summer (June-July in farm A and August in farm B). K. septempunctata DNA was also detected in the polychaetes Naineris laevigata intestinal samples, showing decreased pattern of 40 to 0%. No actinosporean stage of K. septempunctata was observed in the polychaetes by microscopy. The absence of K. septempunctata DNA in rearing water of fish farm and the polychaetes N. laevigata intestinal samples during late spring and early summer indicate that the infection may not occur during this period. N. laevigata was suspected as the possible alternate invertebrate host of K. septempunctata, but the actinosporean stage was not found by well plate method and further studies will be necessary. This research provides important baseline information for understanding the infection dynamics of K. septempunctata in olive flounder farms and further establishment of control strategies.

• The Journal of the Korea Contents Association
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• v.13 no.2
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• pp.488-497
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• 2013

The purpose of the study was to investigate the relationship between the degree of participation and flow for participants in ground, water, and air leisure sports-based tourism activities. A total of 437 participants' data was employed for the analyses (one-way ANOVA and multiple regression analysis) using SPSS Win 18.0 program. Main findings are as follows; First, there were differences in clear goals, transformation of time, loss of self-consciousness, challenge-skill balance, autotelic experience, unambiguous feedback, and sense of control out of flow dimensions based on types of leisure sports-based tourism activities. Second, participation period and frequence had positive effects on flow. Specifically, period had an influence on challenge-skill balance. clear goals: frequence had an effect on challenge-skill balance, transformation of time, clear goals, autotelic experience, unambiguous feedback, and sense of control: intensity had an influence on transformation of time and autotelic experience.

• Journal of Korean Neurosurgical Society
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• v.37 no.1
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• pp.44-47
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• 2005

Objective: The purpose of this study is to investigate the elevated blood glucose levels in the postoperative period are associated with an increased risk of deep wound infection in diabetic individuals undergoing lumbar spine surgery. Methods: Of 2896 patients who underwent lumbar spine operations by one surgeon between 1993 and 2002, 329(11.4%) were diabetics. The rate of deep wound infections in diabetic patients was 6.4%, versus 3.2% for nondiabetics. 152 patients had their operation before implementation of the protocol and 177 after implementation. Charts of the diabetic patients were reviewed. Mean blood glucose levels were calculated from documented results of finger-stick glucometer testing. Results: Twenty-one diabetic patients suffered deep wound infection. Infected diabetic patients had a higher mean blood glucose level through the first 2 postoperative days than noninfected patients($230{\pm}6.9$ versus $175{\pm}3.8mg/dL$; p<0.003) and had a long operation time($216{\pm}57.9$ versus $167.5{\pm}42.2$ minute; p<0.05). Multivariable logistic regression showed that mean blood glucose level for the first 2 postoperative days, long operation time, and use of the instrumentation(p<0.02) were all related predictiors of deep wound infection. Institution of a protocol of postoperative continuous intravenous insulin to maintain blood glucose level less than 200mg/dL was began in september 1997. This protocol resulted in a decrease in blood glucose levels for the first 2 postoperative days and a concomitant decrease in the proportion of patients with deep wound infection, from 8.3%(11/132) to 5.1%(10/195) (p<0.02). Conclusion: The incidence of deep wound infection in diabetic patients is reduced after implementation of a protocol to maintain mean blood glucose level less than 200mg/dL in the immediate postoperative period.

• Journal of Nutrition and Health
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• v.12 no.2
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• pp.99-105
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• 1979

This study was carried out using rats weighing $40{\sim}50\;g$ which were devided into seven groups with various diet compositions emphasizing fat levels of perilla seed oil for the period of 41/2 weeks. The levels of fat in the diet were 5%, 10%, 15% and animals were fed ad libitum. The results are as follows : 1) Yellow pjgmentation of both neck and tail was clearly observed in groups fed 10% and 15% level perilla seed oil without vitamin I supplementation (IV and VII). 2) The growth rate in groups fed 15% level perilla seed oil was reduced as compared to that in groups fed 5% or 10% level perilla seed oil. 3) The mean hematocrit values of 15% level perilla seed oil groups tended to be lower than those of control group, tut the differences were not significant. 4) The serum vitamin I concentration showed different value in various groups, the values of control group were significantly higher than those of perilla seed oil groups-15% level with or without vitamin E supplementation (VI and VII) and 10% level without vitamin E supplementation (IV). According to the results, 10% level-perilla seed oil in the diet can be considered safe if vitamin E is not omitted from the vitamin mixture ana the group fed 15% fat with P/S ratio of 1 appeared to be safe during the experimental period. Finally the long-term studios have to be persued in many aspects by using perilla seed oil in the experimental diet. Because rats are known t4 be quite resistant to the experimental diets, comparative studies using various animal species have to he conducted.

• Restorative Dentistry and Endodontics
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• v.20 no.1
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• pp.372-383
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• 1995

The staining tendency of esthetic restorative material was very important factor for the people who are great concern about the esthetics. Most external stains were superficial and adjustable by routine prophylactic procedure. But some of these stains were remained under superficial stain. Some of these stains were accumulative on external tooth surface and it's removal alter the anatomic contour of restoration. The purpose of this study was to evaluate and compare the staining tendency of esthetic restorative materials to staining solution. In this study two glass-ionomer cements (Fuji II Glass-Ionomer Cement and Fuji II LC Glass-Ionomer Cement) and three composite resins (Sil$\ddot{u}$x Plus, APH and P-50) were evaluated and compared. Total 8 disc-shaped specimens of each material (17mm diameter, Imm thick) were immersed in coffee staining solution. These specimens were divided into one control and 3 experimental groups according to the immersion period as follows : Control: immersed in distilled water during each testing period Group 1 : immersed in staining solution for 6 hours Group 2 : immersed in staining solution for 24 hours Group 3 : immersed in staining solution for 72 hours Staining tendency was evaluated by total color difference(${\Delta}E^*$) of specimen before and after staining by spectorcolorimeteric readings (ColorQUEST Spectrophotometer, U.SA.). The results were as follows : 1. The total color differences of each testing materials were increased with time. 2. Among the experimental groups, the Fuji II Glass Ionomer Cement showed the highest total color difference(6.803) and the Silux Plus showed the lowest total color difference(1.637). 3. In comparison of glass ionomer cements, the total color difference of chemical cured glass ionomer cements(6.803) were higher than light cured glass ionomer cements(3.891) (P<0.01). 4. In comparison of composite resins, the P-50 showed the highest total color difference and the Silux Plus showed the lowest total color difference, but there was not significant difference among composite resins(P>0.05).

• The Korean Journal of Rehabilitation Nursing
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• v.6 no.1
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• pp.70-78
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• 2003

This study was a quasi-experimental study of nonequivalent control group pretest-posttest design to investigate the effect of rhythmic dance movement training on the physical and psychological functions of the elderly. The data were collected from November, 2001 to February, 2002. The subjects for this study were 34 elderly who was over 65 years old and was living in J city. The elderly selected for this study were: free from heart and pulmonary disease and not regular exercise. The rhythmic dance movement training in watching video tape was rhythmic dance movement and education and supportive care. The rhythmic dance movement was 40-60 intensity, 8 weeks' period, three times a week, 60 minutes a day. The data were analysed by $X^2$-test, paired t-test and unpaired t-test and ANCOVA through SAS/PC program. The results of the study were as follows: 1. There was insignificant difference in the gait length experimental and control group. 2. There was significant difference in the gait speed between the two groups. 3. There was significant difference in the dynamic valance between the two groups. 4. There was no significant difference in the depression between the two groups. 5. There was no significant difference in the Quality of life between the two groups. As shown above, the results of the 8 weeks' rhythmic movement program for the elderly produced positive effects on gait speed, dynamic valance. And this program was expected that it was more effective in different intervention period, verified program. Also it was needed follow study.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.361-374
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• 2006

This study was conducted to evaluate the effect of fermented food waste (FFW) supplementation on growth, nutrient digestibility and pork quality of growing pigs. A total of 48 crossbred pigs were assigned to four treatments in a randomized complete block (RCB) design. Treatments were 1) Control (basal), 2) FFW 2% (basal+2%FFW), 3) FFW 4% (basal+4%FFW) and 4) FFW 6% (basal+6%FFW). Diets were formulated based on corn-soybean meal and the FFW products were provided to weaning, growing and finishing periods. During the whole experimental period, FFW treatment groups showed similar ADG, ADFI and G:F ratios (P>.10) compared to control group. However, In the finishing period, pigs fed 6% FFW tended to be lower ADG than other dietary treatments (P=0.13) resulting in lower final weight. All pigs showed inconsistent blood urea nitrogen (BUN) concentrations pattern. In metabolic trial, nutrient digestibility was not affected by the supplementation of FFW. The pork quality was affected by the supplementation of FFW based upon pork pH, lipid peroxidation (TBARS) and meat color analysis. These results suggested that utilization of fermented food waste to growing pig's diet reduce production cost without any detrimental effect on pork quality when it was provided at low level.

• Microbiology and Biotechnology Letters
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• v.1 no.1
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• pp.25-30
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• 1973

Experiments were carried out to clarify changes of intercellular gas composition, respiration and ethylene evolution by controlled external atmospheric pressure and external gas composition on apples. Jonathan apples, harvested 25, in September and Rolls apples, harvested 25, in October, 1971 and 1972 were used. Results obtained were as follows: 1. Intercellular gas volumes were proportionally changed by the given pressure, and the given pressure remarkably affected internal gas composition and volume of apples. As intercellular gas volumes were reached in their equilibrium within 5 min. after treatment, the internal atmospheric conditions became constant rapidly. 2. The increase of internal $CO_2$ production was co-related with an amount of internal $O_2$ consumption, therefore, the decreasing period of internal $O_2$ consumption was equalled to the period of climacteric rise in respiration. 3. The increasing of $CO_2$ production followed evolution of $C_2$H$_4$ and this phenomenon on SAP part. was subsequent to NAP part 4. In sub-atmospheric storage, CA effect was also obtained by control of low $O_2$ and high $CO_2$ concentration.

• Journal of Korean Society on Water Environment
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• v.25 no.6
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• pp.821-831
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• 2009

The study was aimed to assess the expected impact of climate change on the water cycle and soil losses in Daecheong Reservoir watershed, Korea using the Soil and Water Assessment Tool (SWAT) that was validated for the watershed in a previous study. Future climate data including precipitation, temperature and humidity generated by introducing a regional climate model (Mesoscale Model Version 5, MM5) to dynamically downscale global circulation model (European Centre Hamburg Model Version 4, ECHAM4) were used to simulate the hydrological responses and soil erosion processes in the future 100 years (2001~2100) under the Special Report on Emissions Scenario (SRES) A1B. The results indicated that the climate change may increase in the amount of surface runoff and thereby sediment load to the reservoir. Spatially, the impact was relatively more significant in the subbasin Bocheongcheon because of its lower occupation rate of forest land compared to other subbasins. Seasonally, the increase of surface runoff and soil losses was more significant during late summer and fall season when both flood control and turbidity flow control are necessary for the reservoir and downstream. The occurrence of extreme turbidity flow events during these period is more vulnerable to reservoir operation because the suspended solids that remained water column can be resuspended by vertical mixing during winter turnover period. The study results provide useful information for the development of adaptive management strategy for the reservoir to cope with the expected impact of future climate change.

• Journal of Animal Science and Technology
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• v.54 no.3
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• pp.157-163
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• 2012

The male reproduction is precisely controlled by a number of intrinsic and extrinsic factors. These factors usually involve in expressional regulation of various molecules influencing on sperm production in the testis. A number of ways are employed to control the transcription of specific genes, including epigenetic modifications of DNA and histone molecules. DNA methylation of CpG dinucleotides is a commonly used regulatory mechanism for testicular genes associated with the fertility. Previous studies have demonstrated the infertility induced by improper DNA methylation of these genes. In the present research, we attempted to determine transcriptional expression of some of these genes in the rat testis at different postnatal ages using real-time PCR analysis. These genes include neurotrophin 3 (Ntf3), insulin-like growth factor II (Igf2), JmjC-domain-containing histone demethylase 2A 1 (Jhm2da), paired box 8 transcription factor (Pax8), small nuclear ribonucleoprotein polypeptide N (Snrpn), and 5,10-methylenetetrahydrofolate reductase (Mthfr). The expression levels of Ntf3, Igf2, and Snrpn genes were the highest at the neonatal age, followed by transient decreases at the prepubertal age. Expression of Jhm2da and Mthfr genes were continuously increased from the neonate to 1 year of age. The levels of Pax8 mRNA at the early ages were higher than those at the later ages of postnatal development. These findings suggest that expression of some fertility-associated testicular genes in the rat during postnatal period could be differentially regulated by the control of the degree of DNA methylation.