• Journal of Korean Society of Transportation
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• v.19 no.2
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• pp.89-98
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• 2001

The objective of this Paper is to derive an adjustment factor for the presence of heavy vehicles when estimating capacity at unsignalized intersections (and/or at modern roundabouts). According to the 1997 and 2000 Highway Capacity Manual (HCM), potential capacity in such cases is estimated by simply adjusting base critical gap and base follow-up time. However, the procedure suggested in the HCM may lead to some errors in the adjustment, hence resulting in poor evaluation and design for the intersections, because it determines the value of adjusting factors by only the number of lanes on main streets regardless of the types of heavy vehicles. This paper shows a simple formula for making the adjustment. This formula is much like the HCM formula used for heavy vehicles in estimating highway capacity by the adoption of passenger car units (PCU). In contrast to the traditional approaches seen in the HCM, the PCU value of this case is explicitly expressed by the flow rate in the major streams and the gap difference in critical gaps chosen by passenger cars and particular heavy vehicles. Computational results of the adjustment factor are graphically illustrated.

• Journal of Chest Surgery
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• v.32 no.8
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• pp.693-701
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• 1999

Recently non-invasive diagnostic imaging replaced the invasive catheter angiography in the diagnosis of vascular disease. Catheter methods are now almost confined to the purpose of intervention. Coronary artery or coronary artery bypass graft still needs catheter technique because of small diameter and the cardiac motion. The last challenge for radiologists in this domain is to obtain a non-invasive imaging. Electron beam tomography(EBT) for high temporal resolution is able to obtain a coronary arteriogram or coronary artery bypass graft (CABG), of which CABG imaging is quite useful for the evaluation of patency. In our experience as well as others, the accuracy of EBT angiogram in evaluating CABG patency revealed that the accuracy of patency of saphenous vein grafts(SVG) is high due to relatively wide lumen, short and straight course and less influence from cardiac motion. The sensitivity and specificity of patency of SVGs were 92%, 97% respectively in the prospective evaluat on and 100% each in the retrospective evaluation. A false positive and a false negative case are rudimentary errors in the initial learing period. In contrast the analysis of left internal mammary artery(LIMA) graft was difficult due to the inherent small size and the adjacent surgical clips provoking beam-hardening artifact; therefore, the method of combining 3 dimensional reconstruction and flow mode study was important in improving the accuracy of LIMA patency. The sensitivity and specificity of LIMA patency were 100% and 80% in both prospective and retrospective evaluation. Therefore, EBT angiography is an accurate non-invasive diagnostic modality for evaluating the patency of CABG, particularly in SVGs. The accuracy can be improved with the improvement of the EBT and the development of the image reconstruction software.

• Archives of design research
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• v.18 no.2 s.60
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• pp.111-122
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• 2005

Public Service Advertising(PSA) is the integrated marketing concept including strategy and technology in all its aspects that pursue the changes of community to seek an agreement of its members by the intentional and the target oriented way. PSA to the exclusion of commercial intention reflects the current social flow and subject since it is focused to the social issue. PSA plays an important role in creating the further cultural value, and also affects present cultural value to advertising message. In this aspect, this study is very valuable to design the plan for further effective management of advertisement and to analyze communication strategy of PSA. This study is to make dear the nature of PSA by the analysis of contents of visual image and linguistics' factors in actual produced and broadcasted TV advertising, called 'Protection of environment' In the results, PSA related the environment is working to linguistic-centered persuading message corresponding to visual factor, this intends to educate and instruct the consumers in 1980's. PSA, therefore, shows a non-description nature without story line and a hero(heroin) on it. In contrast, after 1990's, PSA was made up image-centered and maximized the effectiveness public campaign through the activating consumer's judgement and intervention. We are able to know that it contributes to considering and persuading the consumer to suggest the story format through the visual way to deliver the message. This study of relationship between visual image and linguistics is a common trend appeared in all media including today's advertising, and may be a remarkable result to present proper direction of PSA campaign.

• The Journal of Engineering Geology
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• v.25 no.1
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• pp.67-81
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• 2015

A FORTRAN program was developed to determine the optimal locations of multiple recharge wells in an aquifer with different arrangements of pumping wells. The simulated annealing algorithm was used to find optimal locations of two recharge wells which satisfied three objective functions. The model results show that locating two injection wells inside the cluster of pumping wells is efficient if the recovery rate only was taken into account. In contrast, placing injection wells to the side of the cluster is desirable if the simulation considers aggregate objective function. Therefore, installing an injection well on each side of the cluster seems to yield the maximum recovery rates for the existing pumping wells, and it yields similar increases in pumping rate for all wells in the cluster. The locations of recharge wells can be arranged in numerous configurations, because there are multiple near-optimal local minima or maxima. These results indicate that the simulated annealing can yield effective evaluations of the optimal locations of multiple recharge wells. In addition, the suggested aggregate objective function can be utilized as an appropriate multi-objective optimization.

• The Korean Journal of Pharmacology
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• v.28 no.1
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• pp.1-9
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• 1992

Catecholamines, serotonin and their metabolites were measured in the posterior hypothalamus of urethane-anesthetized normotensive Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) using brain microdialysis which is a recently developed experimental method to measure the release of neurotransmitters and their metabolites at the localized brain area in vivo. Microdialysis probe was implanted stereotaxically to the rat posterior hypothalamus and perfused by Ringer's solution. Monoamines and their metabolites were quantified by reverse phase high performance liquid chromatography with electrochemical detection. In vitro recovery test of microdialysis showed that there exist inverse relationship between the perfusion flow rate and the relative recovery of neurochemical compounds. The estimated extracellular concentration of dopamine was about 32 nM, of norepinephrine 50 nM, of epinephrine 50 nM, of serotonin 73 nM, of 3, 4-dihydroxyphenylacetic acid (DOPAC) 281 nM, of homovanillic acid (HVA) 181 nM, and of 5-hydroxyindoleacetic acid (5HIAA) 3767 nM in the hypothalamic perfusate of the normotensive rat. There was no difference in the basal level of monoamines between the SHR and the WKY. In contrast, the level of DOPAC, HVA and 5HIAA in SHR was higher than that in the WKY, This study demonstrated that the microdialysis technique should be an applicable tool for in vivo measurement of central neurochemical substances.

• Journal of the Korean Society of Marine Environment & Safety
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• v.23 no.5
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• pp.549-557
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• 2017

Numerical studies have been carried out on scrubbers, which are after-treatment devices to satisfy strengthened emission regulations for sulfur dioxide and particulate matter. We investigated the problems with existing scrubbers through numerical analysis and designed and analyzed a new swirl-type scrubber that could solve these problems. As a result, with the swirl-type scrubber, exhaust gas formed a vortex in the lower part of the device, and some of this gas was released along the guide vane through the bottom surface. In this case, the pressure gradient in the vertical direction was not large, but a pressure difference between the inside and outside of the baffle was generated. The shape of the exhaust gas stream was investigated, and when water was not sprayed, the exhaust gas flowed constantly to the outlet along the guide vane, in contrast to when water was sprayed. It was confirmed that the shape of the flow was influenced by the guide vane, nozzle arrangement and water pressure. In the case of the swirl-type scrubber, impact on engine back-pressure was minimal, because differential pressure at the inlet and outlet was less than half of that with a conventional scrubber.

• Journal of the Korean earth science society
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• v.37 no.1
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• pp.62-77
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• 2016

In the early model of plate tectonics, the plate was depicted as a passive raft floating on the convecting mantle and carried away by the mantle flow. At the same time, ridge push at spreading boundaries and drag force exerted by the mantle on the base of lithosphere were described as the dominant driving forces of plate movements. However, in recent studies of plate tectonics, it is generally accepted that the primary force driving plate motion is slab pull beneath subduction zones rather than other forces driven by mantle convection. The current view asserts that the density contrast between dense oceanic lithosphere and underlying asthenosphere is the substance of slab pull. The greater density of oceanic slab allows it to sink deeper into mantle at trenches by gravitational pull, which provides a dominant driving force for plate motion. Based on this plate tectonics development, this study investigated the contents of plate tectonics in high school Earth Science textbooks and how they have been depicted for the last few decades. Results showed that the early explanation of plate movement driven by mantle convection has been consistently highlighted in almost all high school textbooks since the 5th curriculum, whereas most introductory college textbooks rectified the early theory of plate movement and introduced a newly accepted theory in revised edition. Therefore, we suggest that the latest theory of plate tectonics be included in high school textbooks so that students get updated with recent understanding of it in a timely manner.

• IMMUNE NETWORK
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• v.2 no.4
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• pp.217-222
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• 2002

Background: Scid.adh is a recently developed murine thymic lymphoma cell line, which has been used as in vitro model for the study of double negative stage III thymocytes. In this study, we compared the expression profile of a number of genes and proteins, which are tightly related to T cell development and apoptosis, in thymic lymphoma cell lines, R1.1, EL-4, and Scid.adh for the developmental staging. Methods: We examined the expression of development marker genes and proteins in three lymphoma cell lines by flow cytometry and RT-PCR. In addition, the expression of apoptosis-related molecules including bcl-2, bax and Fas was also investigated. Results: As previously reported, Scid.adh cell line expressed CD8 and CD25 but not TCR ${\alpha}$ chain, while R1.1 cells expressed TCR ${\alpha}$ chain and both CD4 and CD8 transcripts. These suggest that R1.1 might be in double positive stage, and low level of CD44 expression and the absence of CD25 support this suggestion. In contrast, EL-4 cells showed high level of TCR ${\alpha}$ chain transcript, and low-level of CD4 expression, suggesting that EL-4 is in more mature stage than R1.1. Further, this suggestion was supported by the lack of mT-20 in EL-4 cells, which is expressed in the immature thymocytes, and Scid.adh and R1.1 cell lines, but not in the terminally differentiated thymocytes and peripheral T cells. Among the apoptosis-related gene, transcripts of bcl-2 gene were detected in both R1.1 and EL-4 but not in Scid.adh cells, while bax was expressed in all cell lines. Fas expression was the highest in EL-4 cells and low in Scid.adh cell line. Conclusion: R1.1 cell may represent double positive stage, and EL-4 is more differentiated cell line. In addition, Scid.adh and EL-4 cell lines are suspected to be useful for the study of function of bcl-2 family and Fas during the thymocyte development, respectively.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.4037-4043
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• 2012

Despite progress in elucidating mechanisms associated with colorectal cancer and improvement of treatment methods, it remains a frequent cause of death worldwide. New and more effective therapies are therefore urgently needed. Recent studies have shown that immunogenicity of whole ovarian tumor cells and subsequent T cell response were potentiated by oxidation modification with hypochlorous acid (HOCl) in vitro and ex vivo. These results prompted us to investigate the protective antitumor response with an HOCl treated CT26 colorectal cancer cell vaccine in an in vivo mouse model. Administration of HOCl modified vaccine triggered robust antitumor immunity to autologous tumor cells in mice and prolonged survival period significantly. In addition, increased necrosis and apoptosis were found in tumor tissue from the oxidation group. Interestingly, ELISPOT assays showed that specific T cell responses were not elicited in response to the immunizing cellular antigen, in contrast to raising sera antibody titer and antibody binding activity shown by ELISA assay and flow cytometry. Further evaluation of the mechanisms underlying HOCl modified vaccine mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results combined with previous studies suggest that HOCl oxidation modified whole cell vaccine has wide applicability as a cancer vaccine because it can target both T cell- and B cell-specific responses. It may thus represent a promising approach for the immunotherapy of colorectal cancer.

• Development and Reproduction
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• v.7 no.2
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• pp.105-112
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• 2003

Previously, we discovered a new MMP-2 isoform GA110, of which appearance in human follicular fluid(FF) and serum was increased by EDTA. The present study was conducted to investigate how GAI 10 can appear by EDTA. To examine possible involvement of protein disulfide isomerase(PDI), an enzyme responsible for the dimerization of protein via disulfide formation, effect of PDI inhibitor on the appearance of GA110 by EDTA was investigated. When PDI inhibitor added to FF before EDTA treatment, the gelatinolytic activity of GA110 was abolished in a concentration dependent manner. By contrast, the activity of 72 kDa gelatinase increased. However, the PDI inhibitor added to FF after EDTA treatment, the gelatinolytic activity of GA110 was unaffected. To find out the nature of the enzyme which converts 72 kDa gelatinase into GAI 10, chromatographic separation method of FF proteins was done. Using hydroxyapatite column, fractions rich in 72 kDa gelatinase were isolated and pooled. By using this pool as substrate for the 72 kDa converting enzyme, protein fractions containing the converting activity were obtained from chromatographic separation of FF onto glutathione sepharose fast flow column. When immunoblotting was performed on this enzymatically active protein fractions against polyclonal anti-PDI antibody, distinct immunoreactivity was observed, although appeared in smaller molecular weight region. Based on these observations, it is suggested that the appearance of GAI 10 in FF by EDTA treatment could be due to an activation of PDI-like enzyme, which dimerizes 72 kDa gelatinase into GAI 10 via the formation of disulfide bond between molecules.