• Title/Summary/Keyword: continuous method

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Whole-Genome Sequencing-based Antimicrobial Resistance and Genetic Profile Analysis of Vibrio parahaemolyticus Isolated from Seafood in Korea (유통 수산물에서 분리한 Vibrio parahaemolyticus의 항생제 내성 및 전장 유전체 분석을 통한 유전적 특성 분석)

  • Gyeong Gyu Song;Hyeonwoo Cho;Yeona Kim;Beomsoon Jang;Miru Lee;Kun Taek Park
    • Journal of Food Hygiene and Safety
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    • v.39 no.3
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    • pp.231-238
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    • 2024
  • Vibrio parahaemolyticus is a major seafood-borne pathogen commonly detected in marine environments. In Korea, V. parahaemolyticus-induced foodborne illnesses account for 7.5% of bacterial pathogen-related food poisonings. Moreover, the amount of antimicrobial agents used in aquatic cultures is continuously increasing. In this study, we isolated V. parahaemolyticus from seafood samples and performed antimicrobial susceptibility tests using the microbroth dilution method. Furthermore, using whole-genome sequencing, we identified antimicrobial resistance genes, virulence genes, and sequence types (STs). We could isolate V. parahaemolyticus from 47 (59.5%) of the 79 seafood samples we purchased from retail markets in Seoul and Chungcheong provinces. Antimicrobial susceptibility tests revealed that 2 and all of the 47 isolates were ampicillin-resistant (4.3%) and susceptible to all tested antimicrobial agents (100%), respectively. The genotype analysis revealed that all isolates carried beta-lactam-, tetracycline-, and chloramphenicol-associated antimicrobial resistance genes. However, we could detect fosfomycin resistance only in one isolate. Concerning the virulence genes, we detected T3SS1 and T3SS2-associated genes in all and one isolate, respectively. However, we could not detect the tdh and trh genes. Of the 47 isolates, 17 belonged to 15 different STs, including ST 658 with 3 isolates. The rest 30 isolates were identified as 25 new STs. The results of this study support the need for operating a continuous monitoring system to prevent foodborne illnesses and the spread of antimicrobial resistance genes in V. parahaemolyticus.

Understanding the Asymptotic Convergence of Domain of Attraction in Extreme Value Distribution for Establishing Baseline Distribution in Statistical Damage Assessment of a Structure (통계적 구조물 손상진단에서 기저분포 구성을 위한 극치분포의 점근적 수렴성 이해)

  • Kang, Joo-Sung;Park, Hyun-Woo
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.13 no.2 s.54
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    • pp.231-242
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    • 2009
  • The baseline distribution of a structure represents the statistical distribution of dynamic response feature from the healthy state of the structure. Generally, damage-sensitive dynamic response feature of a structure manifest themselves near the tail of a baseline statistical distribution. In this regard, some researchers have paid attention to extreme value distribution for modeling the tail of a baseline distribution. However, few researches have been conducted to theoretically understand the extreme value distribution from a perspective of statistical damage assessment. This study investigates the asymptotic convergence of domain of attraction in extreme value distribution through parameter estimation, which is needed for reliable statistical damage assessment. In particular, the asymptotic convergence of a domain of attraction is quantified with respect to the sample size out of which each extreme value is extracted. The effect of the sample size on false positive alarms in statistical damage assessment is quantitatively investigated as well. The validity of the proposed method is demonstrated through numerically simulated acceleration data on a two span continuous truss bridge.

CNN Model-based Arrhythmia Classification using Image-typed ECG Data (이미지 타입의 ECG 데이터를 사용한 CNN 모델 기반 부정맥 분류)

  • Yeon-Suk Bang;Myung-Soo Jang;Yousik Hong;Sang-Suk Lee;Jun-Sang Yu;Woo-Beom Lee
    • Journal of the Institute of Convergence Signal Processing
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    • v.24 no.4
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    • pp.205-212
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    • 2023
  • Among cardiac diseases, arrhythmias can lead to serious complications such as stroke, heart attack, and heart failure if left untreated, so continuous and accurate ECG monitoring is crucial for clinical care. However, the accurate interpretation of electrocardiogram (ECG) data is entirely dependent on medical doctors, which requires additional time and cost. Therefore, this paper proposes an arrhythmia recognition module for the purpose of developing a medical platform through the analysis of abnormal pulse waveforms based on Lifelogs. The proposed method is to convert ECG data into image format instead of time series data, apply visual pattern recognition technology, and then detect arrhythmia using CNN model. In order to validate the arrhythmia classification of the CNN model by image type conversion of ECG data proposed in this paper, the MIT-BIH arrhythmia dataset was used, and the result showed an accuracy of 97%.

Nickel dust-induced occupational contact dermatitis by welding and grinding work in shipyard workers: a report of nine cases

  • Daehwan Kim;A Ram Kim;Hanjun Kim;Sunghee Lee;Byeonghak Seo;Ho Seok Suh;Chang Sun Sim;Heun Lee;Cheolin Yoo
    • Annals of Occupational and Environmental Medicine
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    • v.34
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    • pp.7.1-7.10
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    • 2022
  • Background: Occupational skin diseases are skin conditions that occur or worsen in relation to work and known to be the second most common type of occupational disease affecting individuals in the United States. In Korea, epidemiological reports related to occupational skin diseases are rare. But, no cases of occupational contact dermatitis caused by welding and grinding work have been reported previously. Case presentation: Nine male workers working in the production department for liquefied natural gas (LNG) ships in Ulsan complained of erythematous papules/patches and itching in various areas of the body after welding and grinding work. The work environment monitoring report revealed that the amount of nickel dust exceeded the time weighted average (TWA) and poor local ventilation status. Based on the symptoms and the overall results of surveys, several tests, and work environment monitoring report, the 2 workers who had positive patch-test reactions to nickel were diagnosed with nickel dust-induced allergic contact dermatitis. The other 7 workers were diagnosed that there was a high probability that they had nickel dust-induced irritant contact dermatitis. The 2 workers who had nickel dust-induced allergic contact dermatitis were recommended to switch their jobs. Conclusions: Nickel is one of the most common cause of allergic contact dermatitis. In this case, the dust was assumed to be created by welding work with a high nickel content new welding rod and subsequent grinding work, and the concentration of this dust exceeded the time weighted average. Thus, it is thought that the nickel dust may have caused contact dermatitis through continuous contact with the workers' exposed skin in a poorly ventilated space. Currently, several domestic shipbuilding companies are manufacturing LNG tankers using a new construction method. Consequently, it is highly likely that similar cases will occur in the future, which makes this case report meaningful.

A Study on the Real-time Recommendation Box Recommendation of Fulfillment Center Using Machine Learning (기계학습을 이용한 풀필먼트센터의 실시간 박스 추천에 관한 연구)

  • Dae-Wook Cha;Hui-Yeon Jo;Ji-Soo Han;Kwang-Sup Shin;Yun-Hong Min
    • The Journal of Bigdata
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    • v.8 no.2
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    • pp.149-163
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    • 2023
  • Due to the continuous growth of the E-commerce market, the volume of orders that fulfillment centers have to process has increased, and various customer requirements have increased the complexity of order processing. Along with this trend, the operational efficiency of fulfillment centers due to increased labor costs is becoming more important from a corporate management perspective. Using historical performance data as training data, this study focused on real-time box recommendations applicable to packaging areas during fulfillment center shipping. Four types of data, such as product information, order information, packaging information, and delivery information, were applied to the machine learning model through pre-processing and feature-engineering processes. As an input vector, three characteristics were used as product specification information: width, length, and height, the characteristics of the input vector were extracted through a feature engineering process that converts product information from real numbers to an integer system for each section. As a result of comparing the performance of each model, it was confirmed that when the Gradient Boosting model was applied, the prediction was performed with the highest accuracy at 95.2% when the product specification information was converted into integers in 21 sections. This study proposes a machine learning model as a way to reduce the increase in costs and inefficiency of box packaging time caused by incorrect box selection in the fulfillment center, and also proposes a feature engineering method to effectively extract the characteristics of product specification information.

A study on the direction of Multicultural Faith Community Education in Korean churches : Focusing on the Holistic mission of Elizabeth J. Shepping (한국교회 다문화 신앙공동체교육의 방향성 연구 : 서서평의 통전적 선교를 중심으로)

  • Young Mi Kim
    • Journal of Christian Education in Korea
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    • v.78
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    • pp.137-151
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    • 2024
  • Purpose of Research: This study aims to gain insights into the direction of multicultural faith community education, which is a part of multicultural Christian education that contemporary Korean churches should establish, based on the holistic mission practices of missionary Elizabeth J. Shepping. Research Content and Method: The study begins with a brief overview of the life of missionary Elizabeth J. Shepping, who experienced multicultural living in various countries, highlighting the prepared professional missionary within the context of her time. The study then examines the details of her holistic mission practices as a professional missionary. The direction of multicultural faith community education in Korea is explored by understanding the background and research trends of multicultural Christian education that Korean society and the Korean church are currently facing. Practical aspects, supplementary points, and directions for the future implementation of multicultural faith community education in the Korean church are also proposed. Results and Conclusions: In conclusion, This study emphasizes multicultural faith community education as a component of the pastoral paradigm that the Korean church needs to improve. Four practical directions are proposed: first, education for holistic service; second, education for social and public practice; third, implementation of public service education linked to the local community; and fourth, activation of dedicated service departments and training of professional leaders. However, the study acknowledges a limitation in not providing specific practical programs for multicultural faith community education suitable for the Korean church context. Continuous research is necessary through various supplementary points in the future.

Molecular Genetic Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Nasal Cavity of University Students (대학생들의 비강으로부터 분리된 메티실린 내성 황색포도알균의 분자유전학적 특성)

  • Lee Eun gwang;Oh Dae Hwan;Sunjin Jung;Sohyun Park;Yeonim Choi
    • Journal of the Health Care and Life Science
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    • v.9 no.2
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    • pp.303-308
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    • 2021
  • Staphylococcus aureus and methicillin resistant S. aureus (MRSA) is a major cause of nosocomial infections and is one of the most commonly isolated bacterial species in the hospital and continues to be an important pathogens in both community and hospital-acquires infection. The purpose of this study is to investigate the carrier rate of S. aureus and MRSA in the community and molecular genetic characteristics of these organisms. The identification of S. aureus and MRSA were done by the procedures in Murray's manual of Clinical Microbiology and antibiotic susceptibility patterns by the Clinical and Laboratory Standards Institute(CLSI). MRSA strains were confirms by oxacillin disk diffusion method. forty-six strains (71.9%) of S. aureus were isolated from the nasal specimens of 64 students in health science university. twenty-two strains (22%) of 46 S. aureus were resistant to penicillin and oxacillin. twenty-two strains of the 46 S. aureus isolates were methicillin-resistant Staphylococcus aureus (MRSA). The mecA genes in MRSA were detected by polymerase chain reaction (PCR). Community and nosocomial infections caused by methicillin-resistant Staphylococcus aureus are a significant problem worldwide. There continuous epidemiological study is to investigate the prevalence of MRSA in community acquired infections.

Structural Safety Diagnosis of Plastic Greenhouse Using 3D Scanning Method

  • Byung-hun Seo;Sangik Lee;Jonghyuk Lee;Dongsu Kim;Yejin Seo;Dongwoo Kim;Yerim Jo;Won Choi
    • International conference on construction engineering and project management
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    • 2024.07a
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    • pp.1295-1295
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    • 2024
  • As extreme weather events such as heavy snowfall and typhoon become more frequent, climate change significantly impacts across various worldwide industries. With demands for dealing with this phenomenon, continuous achievements in safety diagnosis have been announced for large structures. Conversely, in agricultural infrastructures having lower risk to human life, there is lack of established safety diagnosis methods. However, considering expansion of high-value smart farm, the importance of plastic greenhouse cannot be overlooked. Therefore, this study aimed to develop on-site diagnosis technique for structural safety of steel structure greenhouse. To build an analysis model, we generated point cloud data of on-site greenhouse using a camera with LiDAR sensor. Subsequently, we extracted points corresponding to pipes using a pre-trained semantic segmentation model, achieving a pipe segmentation accuracy of 78.1%. These points were then converted into 3D frame model, with a location coordinate error of 5.4 cm for nine reference points, as measured by an on-site survey. In FEM structural analysis, nonlinearity of pipe connection was reflected. The loads were determined based on expected wind speed and snow depth in Korea. The structural safety of on-site model was diagnosed more vulnerable with 10.3% higher maximum axial stress, compared with standard model. Through this research, we expect the quantitative safety diagnosis of predicting greenhouse collapse risk. In addition, this technique will enable localized reinforcement strategies within the structure.

A Review of Image Analysis Techniques for Investigating Solute Transport in Porous Media (비파괴적 기법을 활용한 다공성 매체에서의 용질 이동 메커니즘 분석에 대한 고찰)

  • Seonggan Jang;Taeseop Kim;Changmin Kim;Minjune Yang
    • The Journal of Engineering Geology
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    • v.34 no.3
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    • pp.473-496
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    • 2024
  • This study reviewed image analysis techniques used in non-destructive investigations of solute transport mechanisms in porous media during contaminant transport. Commonly employed image analysis methods include X-ray imaging, light-transmission visualization, and light-reflection visualization using ultraviolet or visible light. These techniques provide precise, high-resolution data on solute concentration distributions, fluid flow dynamics, and multiphase systems. Through continuous monitoring without alteration of the experimental setup, they provide accurate insights into solute transport mechanisms. We outline the principles, applications, advantages, and limitations of each method, and explore their contribution to the understanding and prediction of solute transport. We also examine case studies in which these methods have been effectively applied. This review provides a comprehensive understanding of how image analysis techniques can contribute to addressing environmental issues such as groundwater contamination.

Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015

  • Si Hyun Kim;Eun Hee Park; In Yeong Hwang;Hyukmin Lee;Sae Am Song;Miae Lee;Seungok Lee;Soo Young Kim;Jin Ju Kim;Jong Hee Shin;Seong Geun Hong;Kyeong Seob Shin;Sunjoo Kim;Nam Hee Ryoo;Woonhyoung Lee;Sook Jin Jang;Jeong Hwan Shin
    • Annals of Clinical Microbiology
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    • v.22 no.3
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    • pp.55-60
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    • 2019
  • Background Salmonella is an important pathogen that causes gastroenteritis and sepsis in humans. Recently, changes in serotype prevalence and an increase in antimicrobial resistance have been reported. This study investigated the distribution of Salmonella serotypes and determined the antimicrobial susceptibility of various strains. Methods We collected 113 Salmonella isolates other than Salmonella serotype Typhi from 18 university hospitals in 2015. The serotypes were identified by Salmonella antisera O and H according to the Kauffman White scheme. Antimicrobial susceptibility tests for 12 antibiotics were performed using the disk diffusion method or E-test. Results We identified 22 serotypes. Serotype group B (44.2%) was the most common, followed by groups C (34.5%) and D (21.2%). Salmonella I 4,[5],12:i:- (23.0%), S. Enteritidis (16.8%), and S. Typhimurium (12.4%) were the most common species. Resistance rates for ampicillin, chloramphenicol, ceftriaxone, and trimethoprim/sulfamethoxazole were 46.9%, 18.5%, 8.8%, and 5.3%, respectively. The intermediate resistance rate to ciprofloxacin was 29.2%. Six isolates were extended-spectrum β-lactamase (ESBL) producers, including 5 blaCTX-M-15 and 1 blaCTX-M-55. Conclusion There have been changes in the serotype prevalence and antimicrobial resistance of Salmonella in Korea, with a high prevalence of CTX-M 15-positive strains. Continuous monitoring of Salmonella serotypes and antimicrobial resistance is warranted.