• BMB Reports
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• v.38 no.3
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• pp.366-369
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• 2005

NADPH-cytochrome P450 reductase (CPR) transfers electrons from NADPH to cytochrome P450 and also catalyzes the one-electron reduction of many drugs and foreign compounds. Various spectrophotometric assays have been performed to examine electron-accepting properties of CPR and its ability to reduce cytochrome $b_5$, cytochrome c, and ferricyanide. In this report, reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by CPR has been assessed as a method for monitoring CPR activity. The principle advantage of this substance is that the reduction of MTT can be assayed directly in the reaction medium by a continuous spectrophotometric method. The electrons released from NADPH by CPR were transferred to MTT. MTT reduction activity was then assessed spectrophotometrically by measuring the increase of $A_{610}$. MTT reduction followed classical Michaelis-Menten kinetics ($K_m\;=\;20\;{\mu}M$, $k_{cat}\;=\;1,910\;min^{-1}$). This method offers the advantages of a commercially available substrate and short analysis time by a simple measurement of enzymatic activity of CPR.

• Database Research
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• v.34 no.3
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• pp.159-169
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• 2018

Discretization of continuous variables intended to improve the performance of various algorithms such as data mining by transforming quantitative variables into qualitative variables. If we use appropriate discretization techniques for data, we can expect not only better performance of classification algorithms, but also accurate and concise interpretation of results and speed improvements. Various discretization techniques have been studied up to now, and however there is still demand of research on discretization studies. In this paper, we propose a new discretization technique to set the cut-point using Wasserstein distance with considering the distribution of continuous variable values with classes of data. We show the superiority of the proposed method through the performance comparison between the proposed method and the existing proven methods.

• Journal of the Semiconductor & Display Technology
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• v.23 no.3
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• pp.46-51
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• 2024

In modern society, the incidence of various joint diseases such as degenerative arthritis is steadily increasing with the increase of the elderly population. For the effective treatment of these diseases, the demand for CPM medical devices that increase the range of motion of the joint is increasing, and if a patient is unable to perform exercise on his or her own, it plays a role in helping joint recovery through repetitive and continuous manual exercise to prevent joint stiffness, promote blood circulation, and improve tissue flexibility to shorten the recovery period after joint surgery to efficiently proceed with the rehabilitation process. Due to the uncertainty of the angle measurement method currently in use, it is difficult to accurately evaluate the patient's exercise amount required to improve the range of motion, which can negatively affect the reliability of CPM medical devices and the patient's willingness to rehabilitate. Therefore, a new technology using encoders and angle sensors is needed to overcome the problems arising from the method measured using only the existing Potentiometer.

• Journal of the Korean Society for Nondestructive Testing
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• v.7 no.1
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• pp.42-50
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• 1987

Most of significant defects in the pressure boundaries of nuclear power plant we re dispositioned to be monitored periodically every inservice inspection. Due to the difficulty of the defect sizing during operation, it is necessary to develope the continuous flaw monitoring techniques. The Tip Diffraction method, specifically speaking, spot seems to be suitable for flaw monitoring. The optimum conditions of selecting the transducer were 3.5 MHz and 45-57 degree according to compatibility with the defect height. The effective calculation of the defect height was to assume the fact that the incident beam is parallel. This method would be supplemented to ASME method about the defect characterization for the surface flaw.

• Proceedings of the ESK Conference
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• 1997.04a
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• pp.107-113
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• 1997

In this paper, an interfacing method to control rehabilitation assitance system with bio-signal is proposed. Controlling with EMG signals method has certain advantage on signal-collecting, but has some drawbacks in the function resolution of EMG signals because data-processing process is not efficient. To improve function-resolution and to increase the efficiency of EMG signal interfacing with rehabilitation assistance system, Multi-layer Perception which is highly effective with static signal and hidden-Markov model for dynamic signal resolving are fused together. In proposed method. The direction and average speed of the rehabilitation assitance system are controlled by the trajectory control and estimation of the moving direction result from the fused model. From the experiment, proposed GMM and 2-level MLP hybrid-classifier yielded 8.6% perception-error rate, improving function resolution. New acceleration control method constructed with 3 nested linear filter produced continuous acceleration paths without the information of destination point. Thus, the mass output caused by non- continuous acceleration-deceleration was eliminated. In the simulation, the necessary calculation, in the case of multiplication, was reduced by 11.54%.

• Journal of the Korean Institute of Telematics and Electronics
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• v.26 no.10
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• pp.1507-1517
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• 1989

Singular values and their gradients have been used to analyze the stability and sensitivity of continuous multiloop systems. But this method has been limited to the discrete systems. This method is extended in this paper to analyze discrete systems directly in discrete domain. To do this, derived is the relationship in the disrete system between the stability margins and the minimum singular value of the return differene matrix, and also implemented is a method which computes singular value gradients. This method is applied to the lateralattitude control loop of a remotely piloted vehide both in continuous case and discrete case for verification of its utility.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.147.5-147
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• 2001

This paper describes a gait algorithm and a velocity limitation method for a Leg-Wheel Robot. The gait algorithm enables the robot to preserve continuous locomotion even if the velocity command varies extensively. The velocity limitation method restricts the commanded velocity when it exceeds the mechanical limitation of the robot. Combined use of the velocity limitation method with the gait algorithm ensures the continuity of locomotion, and makes the gait pattern efficient with a long step length and low frequency of leg phase change. These methods can be applied to locomotion on unexplored rough terrain even if the range of roughness is unknown.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2003.10a
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• pp.19-24
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• 2003

Recently, various efforts to make more speedy and precision machine tool to improve productivity and also various efforts to solve environmental problem are going on, so that dry cutting in manufacturing industry, which needs environmental conscious design and development of manufacturing technique, is becoming a very important assignment to solve. Because dry cutting does not use cutting fluid, we need other methods that can be used instead of cutting fluid, which does cooling, lubricating, chip washing, and anti-corrosion. Especially, because turning is a continuous work, the consideration of tool life and surface roughness due to continuous heat and poor lubrication is important. The purposes of this paper are the consideration of how well the compressed air can work instead of cutting fluid, and also the development of the method to select the optimum machining condition by the minimum numbers of experiments through the Taguchi method.

• Journal of Electrical Engineering and Technology
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• v.10 no.3
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• pp.971-982
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• 2015

In order to improve the quality of the billet continuous casting, a two-phase orthogonal power supply (TPOPS) for electromagnetic stirrer is researched, which is composed of three-phase PWM rectifier and three-leg inverter. According to the power analysis of system, the ripple of dc-link voltage is analyzed and its analytical expression is derived. In order to improve the performance of electromagnetic stirring, an integrated control method with feedforward control is proposed for PWM rectifier to suppress the fluctuations of dc-link voltage and provide a stable dc source for inverter. According to the simplified equivalent model, a composite current control method is proposed for inverter. This proposed method can combine the merits of feedforward control with feedback control to effectively improve the dynamic output performance of TPOPS. Finally, a 300kVA prototype of TPOPS is developed, and the results have verified the analysis and control method.

• Structural Engineering and Mechanics
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• v.5 no.1
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• pp.59-68
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• 1997

A two-step method is presented for the optimum design of trusses with available sections under stress and Euler buckling constraints. The shape design of the truss is used as a means to convert the discrete solution into a continuous one. In the first step of the method, a continuous solution is obtained by sizing and shape design using an approximate polynomial expression for the buckling coefficients. In the second step, the member sizes obtained are changed to the nearest available sections and the truss is reconfigured by using the exact values for the buckling coefficients. The optimizer used is based on the sequential quadratic programming and the gradients are evaluated in closed form. The method is illustrated by two numerical examples.