• Title/Summary/Keyword: contexts

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Epigenetic control of LTR retrotransposons in plant germline and somatic cells

  • Lee, Seung Cho;Parent, Jean-Sebastien;Ernst, Evan;Berger, Frederic;Grimanelli, Daniel;Martienssen, Robert A.
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.97-97
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    • 2017
  • Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.

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Impacts of Framing and the Interaction with Involvement on Responses toward the Campaign of Helping the Hungry Abroad (국제 기아 돕기 캠페인의 효과에 미치는 긍.부정 프레이밍과 관여도의 영향-상호작용을 중심으로)

  • Lee, Seung-Jo;Yeon, Bo-Young;Koo, Se-Hee
    • Science of Emotion and Sensibility
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    • v.13 no.1
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    • pp.147-162
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    • 2010
  • This study investigates how framing and the interaction with involvement influences responses toward a help campaign. There could be two possible predictions about the results of framing. One is that, because helping is a risk avoidance behaviors, the positive framing would dominant or the other is that, because helping is mainly mediated by negative emotions such as sadness or guilty, the negative framing would be preferred. The present research was to solve the conflicting predictions and was conducted with a experimental design. The participants were exposed to a campaign emphasizing either positive aspects of the help or negative contexts which would be maintained without any help. The results were that the main effect of framing was not existent, but the significant interaction with involvement on responses was found. The interaction of framing and involvement, though, included unexpected inconsistency between the results of attitude and behavioral intention. The theoretical and practical meanings of the results were discussed.

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Different Approaches of Introducing the Division Algorithm of Fractions: Comparison of Mathematics Textbooks of North Korea, South Korea, China, and Japan (분수 나눗셈 알고리즘 도입 방법 연구: 남북한, 중국, 일본의 초등학교 수학 교과서의 내용 비교를 중심으로)

  • Yim, Jae-Hoon;Kim, Soo-Mi;Park, Kyo-Sik
    • School Mathematics
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    • v.7 no.2
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    • pp.103-121
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    • 2005
  • This article compares and analyzes mathematics textbooks of North Korea, South Korea, China and Japan and draws meaningful ways for introducing the division algorithm of fractions. The analysis is based on the five contexts: 'measurement division', 'determination of a unit rate', 'reduction of the quantities in the same measure', 'division as the inverse of multiplication or Cartesian product', 'analogy with multiplication algorithm of fractions'. The main focus of the analysis is what context is used to introduce the algorithm and how much it can appeal to students. This analysis supports that there is a few differences of introducing methods the division algorithm of fractions among those countries and more meaningful way can be considered than ours. It finally suggests that we teach the algorithm in a way which can have students easily see the reason of multiplying the reciprocal of a divisor when they divide with fractions. For this, we need to teach the meaning of a reciprocal of fraction and consider to use the context of determination of a unit rate.

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Korean Mathematics Textbook Analysis: Focusing on a Context of Yungbokhap and on Ways of Integration (중학교 1학년 수학교과서의 실세계 기반 과제 분석: 융복합교육의 맥락과 방식을 중심으로)

  • Moon, Jong-Eun;Park, Mi-Yeong;Ju, Mi-Kyung;Jeong, SooYong
    • School Mathematics
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    • v.17 no.3
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    • pp.493-513
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    • 2015
  • This study is based on the perspective that tasks in local and global contexts are important to raise students' ability to mathematically analyze and solve diverse phenomena and social issues as members of future civil society. Therefore, this study analyzes 880 real world tasks from 13 types of 7th grade textbook based on 2009 revised Curriculum. As a result of analyzing tasks by using 3 types of context category and 9 types of ways as analysis framework and allowing repetitive coding, personal context was most frequent in the context of Younbokhap. In level of Integrated ways of Yungbokhap, sequential model in which other subjects' topics were not directly related to mathematics problem or presented in simple description is most frequent. The result shows that the textbooks are too limited to develop learner's competencies. Through such analysis result, we discuss about the necessity and methods of developing tasks which allow learners to study further in future society and global context and which connect other subjects' knowledge and social issue with mathematics in deeper level.

A Study on the Counter-Measures for International Events through the Case Studies and Its Implications for Counter-Terrorism Policy (국제 행사에 대한 테러대응 사례분석과 정책적 함의)

  • Park, Dong-Kyun;Shin, Ik-Chu
    • Korean Security Journal
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    • no.14
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    • pp.161-179
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    • 2007
  • The numerous definition of terrorism is viewed as the use of force or violence by individual or group that is directed toward civilian populations and intended to instill fear as a means of coercing individuals or groups to change their political or social positions. Recently, the paradigm of terror has been developed as new terrorism motivated by 9. 11 terror in 2001. In these contexts, this study analyzed the case study of recent counter-terrorism of international events and suggested the policy implications. This study is split into four chapters. Chapter I is the introduction part. Chapter II introduces the reader to new terrorism theory, and Chapter III deals with the case study of the international counter-terrorism policy around the world, Chapter IV deals with the policy implications of the case study. The greater the political, economical and social advantages opening large international ceremonies, the larger the probability of being targets for terrorists and criminals. As terrorism is one of the important issue, the security problems at international ceremonies in Korea, rising country as political essence in Asian-Pacific region, become very important. With experienced know-hows against terror and preparations for security, local and central governments must promote the private security companies filling up vacancies of police and official security system and develop international ceremonies, rising high valuable industries in 21st century, with diplomatic efforts. International major events is the largest event related events with politics, economy, culture, and such large-scale events should be a comprehensive counterplan in the light of safety check for the location of a hazard and safety check of facilities in and out, attendance on athlete and visitor and escort of VIPs.

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An Integrated Processing Method for Image and Sensing Data Based on Location in Mobile Sensor Networks (이동 센서 네트워크에서 위치 기반의 동영상 및 센싱 데이터 통합 처리 방안)

  • Ko, Minjung;Jung, Juyoung;Boo, Junpil;Kim, Dohyun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.8 no.5
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    • pp.65-71
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    • 2008
  • Recently, the research is progressing on the SWE(Sensor Web Enablement) platform of OGC(Open Geospatial Consortium) to provide the sensing data and moving pictures collected in a sensor network through the Internet Web. However, existed research does not deal with moving objects like cars, trains, ships, and person. Therefore, we present a method to deal with integrated sensing data collected by GPS device, sensor network, and image devices. Also, this paper proposes an integrated processing method for image and sensing data based on location in mobile sensor networks. Additionally, according to proposed methods, we design and implement the combine adapter. This combine adapter receives a contexts data, and provides the common interface included parsing, queueing, creating unified message function. We verity the proposed method which deal with the integrated sensing data based on combine adapter efficiently. Therefore, the research is expected to help the development of a various context information service based on location in future.

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The International Comparative Study on the Origin of the Terms of Mineral and Rock (광물·암석 용어의 어원에 대한 국제 비교 연구)

  • Lee, Chang-Zin;Ryu, Chun-Ryol;Cho, Jun-Oh
    • Journal of the Korean earth science society
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    • v.32 no.3
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    • pp.306-323
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    • 2011
  • The purpose of this study is to analyse the origin of the terms for well-known minerals and rocks in South Korean, North Korean, English, Chinese and Japanese. All of the terms for mineral and rock have been standardized in English and Chinese, Whereas not all of the terms are standardized yet in Japanese, North Korean and South Korean. Although many Korean researchers have recognized the fact and tried to standardize the terms, most of the terms for minerals and rocks have been used without any research conducted on them, which made it impossible. The terms for minerals were mainly made based on some of 28 geologic properties: chemical composition, color, shape and material. The terms for rocks were mainly originated from some of their 30 geologic properties: mineral composition, origin of rock, material and surface. 23 of the 124 English terms were named after the names of men or location, Whereas only 3 terms were originated from a name of place in the Eastern countries. In the Eastern countries, the terms for some minerals or rocks often consist of more complex geologic properties, compared with English terms for the same ones. For the minerals with complex geological properties, in the Eastern countries, there are 28 terms on average, which is much more than 2 in English. There are 9.25 terms in average for the rocks with complex geological properties in the Eastern while only 5 terms exist in English. Some of the Korean terms are very difficult for students to recognize what they are because the terms were originated from Japanese or English terms, which were translated into Korean without consideration of Korean contexts. Therefore the terms of rocks and minerals need to be discussed about their meaning and relevance.

Role of Home Economics Education in a Low Fertility Society by Community Involvement, Public Policy Participation, and Advocacy (저출산 사회에서 지역사회연계와 정책참여를 통한 가정과교육의 역할)

  • Lee, Gyeong-Suk
    • Journal of Korean Home Economics Education Association
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    • v.24 no.1
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    • pp.73-84
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    • 2012
  • The purpose of this study was to explore the role of home economics education in a low fertility society in the contexts of curriculum contents, community involvement, public policy participation, and advocacy. The results showed following. First, home economics is a key subject matter in dealing with a low fertility problem among 2007 revised curriculum for secondary education level. Home economics curriculum contents was systematically organized through 8th grade to 10th grade in 4 units, where as curricula contents of social studies, science, and moral education deal with a low fertility problem in partial. Second, it is proposed to be active in community involvement incorporating with secondary education, college education, and lifelong education by service learning, outreach program, and extension program. Third, public policy and advocacy participation of home economics teachers are crucial for transformative leadership and practice in enhancing the condition of individual, family, and community wellbeing such as a low fertility problem. To perform this role of home economics education, home economics teachers should participate in developing curricula and teaching materials for the extra curricula activity, creative experiencing activity, and service learning activity. Home economics teacher educators also should actively involved in outreach program and extension program as well as in public policy advocacy activity for solving a low fertility problem.

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A Study on the Entertaining Expressions in the Spaced for Entertainment - With a Focus on the Club Environment - (엔터테이먼트 공간에 나타나는 유희적 표현에 관한 연구 - 클럽 공간을 중심으로 -)

  • Kim, Hye-Hyun;Yoon, Gab-Geun;Mun, Hyok;Seo, Su-Mi
    • Korean Institute of Interior Design Journal
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    • v.20 no.5
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    • pp.42-50
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    • 2011
  • In the 21st century, people began to pay more attention on the culture, and the chances for them to participate in the cultural lives also increased. The spaces for entertainments can also be deemed as the center of the cultural contents. The entertainment is, in consideration of its definition by the literal meaning and the origin of the word itself, 'an act that entertains, or any artificial creation that is designed to give entertainment with a view to relieve the subjects from the burdens they have as they live.'Of the modem entertainment spaces, the one with the most profound visual manifestation of the entertainment element is the club. The purpose of this study is to examine the influence from the entertaining element manifested in the club spaces. Only until a few years back, the club spaces were deemed with negative impressions that comes with the more negative side of the entertaining world. But, recently such negative impressions are lift off considerably, and the club spaces are regarded with positive impressions as to be the kind of place where the young people can enjoy performances and other kind of culture in healthy atmosphere. In Chapter 2, we will examine the entertainment and the nature of the pleasure it gives, as well as the correlation between the entertaining environments and the pleasure they give. In chapter 3, the entertaining elements within such contexts are classified into the experience, form, color, objects, and the lightings and examine the characteristics of each of them. Also, the influences of these elements to the entertaining environment will be reviewed. In the case study in chapter 4, 9 overseas and domestic club spaces that were introduced in 'Interior World' magazines, that is, in the issues that are focused on 'Entertainment,'such as Issue 59, 68, and 79. As a result of the analysis, the influences of the entertaining, or pleasing, elements were discovered. The particular expression characteristics of the pleasing designs in the club spaces are, namely, the form elements, material elements, color elements, objects elements, and the lighting elements. As the society changes and the cultural level goes up, the club spaces are required to adopt more changes. Therefore, the pleasing elements of the space should receive further emphasis. These elements will act as a trend in the space and make the people appreciate the space more refreshing.

Understanding STEM, STEAM Education, and Addressing the Issues Facing STEAM in the Korean Context (STEM, STEAM 교육과 우리나라 융합인재교육의 이해와 해결 과제)

  • Sim, Jaeho;Lee, Yangrak;Kim, Hyun-Kyung
    • Journal of The Korean Association For Science Education
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    • v.35 no.4
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    • pp.709-723
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    • 2015
  • Since 2011, after beginning of the systematic study on STEAM education, South Korea has developed a number of related programs. At this point we see that this is the time to clarify the challenges. The purpose of this study is to clarify the characteristics of their education through the review of many domestic and foreign papers in order to propose the challenges of STEAM education of Korea. The results are as follows. First, the course of integration cannot be separated by the difference in superiority but should be separated in accordance with the purpose of integration. Second, curriculum integration such as STEAM education is characterized by the emphasis on horizontal linking than vertical depth fusion. Accordingly, the content knowledge and vertical linkages are inevitably weakened. In order to overcome this problem, the key concepts and features that were emphasized in the previous curriculum need to also be emphasized in STEAM training, and the comparative study on the core concept and function of each subjects should be preceded. Third, after looking upon the current situation of our country's fusion research and talent training, the limits and the challenges that need to be overcome has been suggested. Fourth, with the basis on research results, we offered an example of the approach on STEAM education which is applicable to the current situation and proposed the challenges and implications that need to be addressed in the STEAM education of Korea in educational contexts such as curriculum, teaching and learning, and evaluation.