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Ginsenoside Rg1 supplementation clears senescence-associated β-galactosidase in exercising human skeletal muscle

  • Wu, Jinfu;Saovieng, Suchada;Cheng, I-Shiung;Liu, Tiemin;Hong, Shangyu;Lin, Chang-Yu;Su, I-Chen;Huang, Chih-Yang;Kuo, Chia-Hua
    • Journal of Ginseng Research
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    • v.43 no.4
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    • pp.580-588
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    • 2019
  • Background: Ginsenoside Rg1 has been shown to clear senescence-associated beta-galactosidase (SA-${\beta}$-gal) in cultured cells. It remains unknown whether Rg1 can influence SA-${\beta}$-gal in exercising human skeletal muscle. Methods: To examine SA-${\beta}$-gal change, 12 young men (age $21{\pm}0.2years$) were enrolled in a randomized double-blind placebo controlled crossover study, under two occasions: placebo (PLA) and Rg1 (5 mg) supplementations 1 h prior to a high-intensity cycling (70% $VO_{2max}$). Muscle samples were collected by multiple biopsies before and after cycling exercise (0 h and 3 h). To avoid potential effect of muscle biopsy on performance assessment, cycling time to exhaustion test (80% $VO_{2max}$) was conducted on another 12 participants (age $23{\pm}0.5years$) with the same experimental design. Results: No changes of SA-${\beta}$-gal were observed after cycling in the PLA trial. On the contrary, nine of the 12 participants showed complete elimination of SA-${\beta}$-gal in exercised muscle after cycling in the Rg1 trial (p < 0.05). Increases in apoptotic DNA fragmentation (PLA: +87% vs. Rg1: +133%, p < 0.05) and $CD68^+$ (PLA:+78% vs. Rg1:+121%, p = 0.17) occurred immediately after cycling in both trials. During the 3-h recovery, reverses in apoptotic nuclei content (PLA:+5% vs. Rg1 -32%, p < 0.01) and increases in inducible nitrate oxide synthase and interleukin 6 mRNA levels of exercised muscle were observed only in the Rg1 trial (p < 0.01). Conclusion: Rg1 supplementation effectively eliminates senescent cells in exercising human skeletal muscle and improves high-intensity endurance performance.

CO and Soot Yields of Wood Combustibles for a Kitchen Fire Simulation (주방 화재시뮬레이션을 위한 목재 가연물의 CO 및 Soot Yields)

  • Mun, Sun-Yeo;Hwang, Cheol-Hong;Kim, Sung-Chan
    • Fire Science and Engineering
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    • v.33 no.1
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    • pp.76-84
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    • 2019
  • Experimental studies using an open cone calorimeter were conducted to provide information on the CO and soot yields of wood combustibles required for a kitchen fire simulation of PBD. A total of eight specimens were examined for medium density fiberboard (MDF) and particle board (PB), which are used widely in kitchen furniture production, depending on the water content, surface processing method, and surface color. The thermal penetration time related to the fire spread rate in the depth direction differed significantly according to the surface processing treatment method, even for a specimen of identical thickness. The CO yield ($y_{CO}$) of the MDF and PB series did not change significantly according to the combustion mode and surface treatment process in flaming mode. On the other hand, $y_{CO}$ was approximately 10 times higher in smoldering mode than in flaming mode. The soot yield ($y_{soot}$), however, varied considerably depending on the combustion mode and surface treatment process. In particular, a higher $y_{soot}$ was found in flaming mode and in the surface-treated specimens. Finally, the $y_{CO}$ and $y_{soot}$ of MDF and PB measured for the kitchen fire simulation of PBD were applied.

Mixed organic acids improve nutrients digestibility, volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high-fiber diet

  • Li, Miao;Long, Shenfei;Wang, Qianqian;Zhang, Lianhua;Hu, Jiangxu;Yang, Jie;Cheng, Zhibin;Piao, Xiangshu
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.6
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    • pp.856-864
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    • 2019
  • Objective: The objective of this study was to investigate effects of mixed organic acids (MOA) on nutrient digestibility, volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high wheat bran diet. Methods: Six crossbred barrows ($Duroc{\times}Landrace{\times}Yorkshire$), with an average body weight $78.8{\pm}4.21kg$, fitted with T-cannulas at the distal ileum, were allotted to a double $3{\times}3$ Latin square design with 3 periods and 3 diets. Each period consisted of a 5-d adjustment period followed by a 2-d total collection of feces and then a 2-d collection of ileal digesta. The dietary treatments included a corn-soybean-wheat bran basal diet (CTR), mixed organic acid 1 diet (MOA1; CTR+3,000 mg/kg OA1), mixed organic acid 2 diet (MOA2; CTR+2,000 mg/kg OA2). Results: Pigs fed MOA (MOA1 or MOA2) showed improved (p<0.05) apparent total tract digestibility (ATTD) of gross energy, dry matter and organic matter, and pigs fed MOA2 had increased (p<0.05) ATTD of neutral detergent fiber compared to CTR. Dietary MOA supplementation decreased (p<0.05) pH value, and improved (p<0.01) concentrations of lactic acid and total volatile fatty acids (TVFA) in ileum compared to CTR. Pigs fed MOA showed higher (p<0.05) concentration of acetic acid, and lower (p<0.05) content of formic acid in feces compared to CTR. Pigs fed MOA1 had increased (p<0.05) concentration of TVFA and butyric acid in feces. Pigs fed MOA1 showed higher concentration of Lactobacillus and lower concentration of Escherichia in feces compared to CTR. Conclusion: Dietary supplementation of MOA 1 or 2 could improve nutrients digestibility, TVFA concentration and intestinal flora in growing-finishing pigs fed high fiber diet.

Effect of night light regimen on growth performance, antioxidant status and health of broiler chickens from 1 to 21 days of age

  • Zhao, R.X.;Cai, C.H.;Wang, P.;Zheng, L.;Wang, J.S.;Li, K.X.;Liu, W.;Guo, X.Y.;Zhan, X.A.;Wang, K.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.6
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    • pp.904-911
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    • 2019
  • Objective: The study was conducted to evaluate the effects of night light regimen on growth performance, antioxidant status and health of Lingnan Yellow broiler chickens from 1 to 21 days of age. Methods: A completely randomized factorial design involved 2 photoperiods (constant lighting [CL], 24 L:0 D and intermittent lighting [INL], 17 L:3 D:1 L:3 D)${\times}2$ light intensities (10 lx and 30 lx). A total of one thousand six hundred and eighty 1-d-old Lingnan Yellow broiler chicks were randomly divided into 4 treatments with 6 replicates (70 birds per replicate). The experiment lasted for 21 d. Results: Photoperiods and light intensities had no effect on average daily gain, feed conversion ratio, and mortality of the broiler chickens (p>0.05). The INL had a significant effect on average daily feed intake (p<0.05) of broiler chickens compared with CL. Photoperiod and light intensity had an interactive effect on melatonin (MT) concentration (p<0.05). At CL, reducing light intensity increased MT concentration; INL birds had higher MT but MT concentration was not affected by light intensity. There was an interactive effect on glutathione peroxidase (GPx) and catalase (CAT) in serum and total antioxidant capability (T-AOC) in liver between photoperiod and light intensity. With the decrease of light intensity, the activities of GPx and CAT in serum and T-AOC in liver increased in CL group (p<0.05). Broiler chickens reared under INL had better antioxidant status and 10 lx treatments had higher activities of CAT in serum than 30 lx (p<0.05). Different photoperiods and light intensities had no effect on malondialdehyde. There was an interaction between photoperiod and light intensity on serum creatine kinase (CK) concentration (p<0.05). At CL, the elevated light intensity resulted in an increase in CK content; INL birds had lower CK concentration especially in low light intensity group. Besides, INL and low light intensity significantly reduced the concentration of serum corticosterone and heat shock protein 70 (p<0.05). Serum immunoglobulin M contents were increased in broiler chickens reared under the INL compared with CL group (p<0.05). Conclusion: Results above suggest that the night light regimen of INL and 10 lx could be beneficial to the broiler chickens from 1 to 21 days of age due to the better health status and electricity savings.

FBX Format Animation Generation System Combined with Joint Estimation Network using RGB Images (RGB 이미지를 이용한 관절 추정 네트워크와 결합된 FBX 형식 애니메이션 생성 시스템)

  • Lee, Yujin;Kim, Sangjoon;Park, Gooman
    • Journal of Broadcast Engineering
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    • v.26 no.5
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    • pp.519-532
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    • 2021
  • Recently, in various fields such as games, movies, and animation, content that uses motion capture to build body models and create characters to express in 3D space is increasing. Studies are underway to generate animations using RGB-D cameras to compensate for problems such as the cost of cinematography in how to place joints by attaching markers, but the problem of pose estimation accuracy or equipment cost still exists. Therefore, in this paper, we propose a system that inputs RGB images into a joint estimation network and converts the results into 3D data to create FBX format animations in order to reduce the equipment cost required for animation creation and increase joint estimation accuracy. First, the two-dimensional joint is estimated for the RGB image, and the three-dimensional coordinates of the joint are estimated using this value. The result is converted to a quaternion, rotated, and an animation in FBX format is created. To measure the accuracy of the proposed method, the system operation was verified by comparing the error between the animation generated based on the 3D position of the marker by attaching a marker to the body and the animation generated by the proposed system.

A Study on the Meaning of School Space: Criticism and Alternatives (학교 공간에 관한 의미 탐색: 비판과 대안)

  • Kim, Dal-Hyo
    • Journal of the Korean Institute of Educational Facilities
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    • v.26 no.4
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    • pp.3-10
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    • 2019
  • In school facilities, space is the basic framework. The structure and arrangement of space will provide the form and feel of school facilities. In order to become a high-quality school facility, it is necessary to focus on the educational, human and ecological aspects of the instructor and learner until the space is conceived, designed and completed. However, even when public education was introduced in the past, it did not become a school space considering this aspect. The school space, which focuses on efficiency and labor production, is a school space that reflects the characteristics of a factory-type school, which has been occupied by a large number until recently. Although efforts to improve the quality of school facilities have been attempted in recent years, there is also a need to pursue more active changes. Future-oriented and progressive school spaces include flexibility, connectivity, individualization, diversity(creativity). In other words, space should be flexible so that it can be used faithfully according to the educational situation, not the fixed and limited school space as in the past. In the future, the school space should be open and securely linked to the place where it is essential to complete community relations with the community. In addition, space should be flexible so that the school can meet the needs of each student as much as possible. And the school space should be transformed from the space design of the past fixed pattern to reflect the close relationship between spatial, psychological, physiological, and behavioral areas. When school space needs to shift away from the past and change in a new future-oriented direction, the remaining tasks should be presented with specific characteristics and content of the direction. And the function of the consignment should be handled by related research. Although the text of this study reveals the characteristics of future-oriented school space, more concrete and empirical research results should be presented by subsequent research at home and abroad. It is necessary to reduce trial and error in creating a future-oriented school space where both professors and learners can be satisfied by analyzing the common points and differences between the results of the study. In order to do this, it is necessary to make efforts to approach such research based on the participation of the subjects who teach and learn directly at the school site.

Evaluating fermentation quality, in vitro digestibility and aerobic stability of a total mixed ration ensiled with different additives on Tibet plateau

  • Dong, Zhihao;Wang, Siran;Zhao, Jie;Li, Junfeng;Liu, Qinhua;Bao, Yuhong;Shao, Tao
    • Animal Bioscience
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    • v.34 no.2
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    • pp.223-232
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    • 2021
  • Objective: To investigate the improvement in utilization efficiency of total mixed ration (TMR) on Tibetan plateau, TMR were ensiled with different additives. Methods: A total of 150 experimental silos were prepared in a completely randomized design to evaluate the six treatments: i) control (without additive), ii) Lactobacillus buchneri (L. buchneri), iii) acetic acid, iv) propionic acid, v) 1,2-propanediol; and vi) 1-propanol. After 90 days of ensiling, silos were opened for fermentation quality and in vitro analysis, and then subjected to an aerobic stability test for 14 days. Results: Treating with L. buchneri, acetic acid, 1,2-propanediol and 1-propanol decreased propionic acid contents and yeast number, whereas increased (p<0.05) pH, acetic acid and ethanol contents in the fermented TMR. Despite increased dry matter (DM) loss in the TMRs treated with 1,2-propanediol and 1-pronanol, additives did not affect (p>0.05) all in vitro parameters including gas production at 24 h (GP24), GP rate constant, potential GP, in vitro DM digestibility and in vitro neutral detergent fibre digestibility. All additives improved the aerobic stability of ensiled TMR to different extents. Specially, aerobic stability of the ensiled TMR were substantially improved by L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol, indicated by stable pH and lactic acid content during the aerobic stability test. Conclusion: L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol had no adverse effect on in vitro digestibility, while ensiling TMR with the additives produced more acetic acid and ethanol, subsequently resulting in improvement of aerobic stability. There is a potential for some fermentation boosting additives to enhance aerobic stability of fermented TMR on Tibetan plateau.

A Study of the DB Design Standard for Submitting Completion Drawings for Auto-Renewal of Underground Facility Information (지하시설물정보 자동갱신을 위한 준공도서 제출 표준DB 설계 연구)

  • Park, Dong Hyun;Jang, Yong Gu;Ryu, Ji Song
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.38 no.6
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    • pp.681-688
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    • 2020
  • The Under Space Integrated Map has been constructed consistently from '15 construction projects until the present time in an effort to implement the "ground sinking prevention method" for the purpose of strengthening underground safety management. The constructed Under Space Integrated Map is utilized to provide information to the person in charge at local government through application of the system of underground information based on the administrative network and to deliver this to specialized underground-safety-effects -evaluation organizations through map extraction based on a floor plan. It suffers from a limitation in its practical use, however, since information is only provided, without promoting a separate renewal project. Although in Section 1 of Article 42 in the Special Law Concerning Underground Safety Management the content pertaining to submission obligations of completion drawings related to underground information including change and renewal are stated explicitly in order to solve this problem, submission is not sufficient since a submission window based only on the administrative network is operated. Accordingly, the Ministry of Land, Infrastructure, and Transport constructed an online system for submitting completion drawings, in an attempt to change the method by which entities involved in underground development directly submitted completion drawings. In this study, a DB standard relating to submitting completion drawings was designed and applied in order to construct an auto-renewal system based on submitted completion drawings, which will be extended to cover the range to underground structures hereafter.

Exploring the change in achievement by the transition of the test mode from paper to computer: Focusing on the national assessment of educational achievement of high school mathematics (종이에서 컴퓨터로의 매체 전환에 따른 평가 결과의 변화 탐색: 고등학교 수학 국가수준 학업성취도 평가를 중심으로)

  • Jung, Hye-Yun;Song, Chang-Geun;Kim, Young-Jun;Lee, Kyeong-Hwa
    • The Mathematical Education
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    • v.61 no.4
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    • pp.597-612
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    • 2022
  • Recently, large-scale mathematics assessments are shifting from traditional paper-based tests to computer-based tests, nationally and internationally. This study explored the mode effect (the difference in student achievement by the change of test mode) according to the types of test items, the technological function reflected in the items, the characteristics of students' computer use, and the computer-based test environment. To this end, we analyzed the results of the 2020 national assessment of educational achievement of high school mathematics conducted on a paper and computer basis. As a result, firstly, the mode effect induced by the mode transition was generally insignificant, but the mode effect was larger in the extended response type than other types. Secondly, there were differences in the mode effect according to the transition to test with computer mode where innovative items were added. Thirdly, the difference between mode effects was statistically significant according to the student's sense of efficacy in computer use. The results of this study suggest that innovative items should be introduced deliberately according to the targeted content and competency in evaluation, and that assessment design and environment preparation need to be carefully developed so that nonessential abilities other than students' mathematical ability or incidental situation do not distort the assessment results.

A Study on the Role of the College to Foster Student Entrepreneurship Contents - Focused on the case of Y College of Dept. Fashion Indestry (학생 창업콘텐츠 육성을 위한 전문대학교의 역할 - Y전문대학교 패션산업과 사례를 중심으로)

  • Lee, Ji-Yeon;Kim, Jang-Hyeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.3
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    • pp.697-706
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    • 2021
  • The purpose of this study is to find out whether the many start-up support programs provided by a college can actually help students start a business, and to discuss what role colleges should have as facilitators of student entrepreneurship. To this end, a qualitative study was conducted with seven students who wished to start a business by utilizing start-up education and support programs provided by College Y. As a result of the study, the participants first mentioned parallel and administrative inefficiency in entrepreneurship and the entrepreneurship programs. Second, the implications for the role of colleges in fostering start-up content are systematic planning and guidance for start-up programs operated by the college, concise administrative procedures, mentoring in various start-up items, and securing a test market. It can be said that the concept of a common office in which students can work together is necessary. The study is significant in that it can help to establish a start-up support plan by suggesting what role colleges should play in relation to entrepreneurship, but its limitation is that its scope includes only one specific major.