• Title/Summary/Keyword: content amino nitrogen

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Protein and Amino Acid Composition of Water Cress Oenanthe stolonifera DC (미나리의 단백질의 및 아미노산 조성)

  • 문숙임;조용계;류홍수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.19 no.2
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    • pp.133-142
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    • 1990
  • This study was attempted to compare the nutritive value of leaf with stem of the water cress Oenanthe stolonifera DC. in order to improve the eating habits and as a part of studying on the effective curing nutrients for the damaged liver. The contents of moisture crude proteinon the effective curing nutrients for the damaged liver. The contents of moisture crude protein crude fat and crude ash were 90.40% 2.85%, 0.42% and 0.74% in leaf while the contents of moisture crude protein crude fat and crude ash were 95.15% 0.77% 0.09%, and 0.64% of moisture crude protein crude fat and crude ash were 95.15% 0.77% 0.09% and 0.64% in stem respectively. The quantitative fractionation of proteini of both leaf and stem ranked albumin the highest content followed globulin prolamin and glutelin in order. It has been sh-own by SDS-polyacrylamide gel electrophoresis that water extractable protein of leaf 11 bands but those of stem were not detected. The scope of molecular weight for the main protein of water extractable protein of leaf was between 34.700 and 45,000. The amounts of extractive-nitrogen from leaf and stem of the water cress were 241.02mg% and 271.67mg% respec-tively. The amounts of free amino acid-nitrogen from the leaf and stem were 89.02mg% and 32.02mg% respectively. In free amino acid-nitrogen from the leaf and stem were 89.02mg% and 32.02mg% respectively. In free amino acid composition of both leaf and stem the major components were aspartic acid and glutamic acid. In total amino acid composition of water cress leaf aspartic and glutamic acid were the major components. Whereas alanine and thr-eonine were the major components in stem The assessment of water cress leaf and stem with chemical score. EAAl Rl showed that the values of stem were lower tendancy than those of leaf. Limiting amino acid of leaf was tryptophan while that of stem was lysine.

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Processing and Quality Characteristics of a Cultured Recessive Small-sized Abalone Haliotis discus hannai Extract (양식 참전복(Haliotis discus hannai) 열성패 추출물의 제조 및 품질특성)

  • Cho, Jun-Hyun;Nam, Hyeon-Gyu;Oh, Kwang-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.6
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    • pp.640-646
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    • 2018
  • To determine whether there are differences in the food component characteristics of the cultured recessive small-sized abalone Haliotis discus hannai (SA; 30-40 each/kg) and middle-sized abalone (MA; 10-15 each/kg), the proximate compositions, fatty acid and total amino acid compositions of these two species were evaluated. Additionally, extraction methods were performed on the SA to asses the quality characteristics of the resulting. In terms of proximate composition, MA had lower moisture and carbohydrate levels and higher crude protein contents than SA. The total amino acid contents of MA and SA were 15,734.4 and 11,379.1 mg/100 g, respectively, the major amino acids were glutamic acid, aspartic acid, serine, glycine, alanine, leucine, arginine and lysine, and the major fatty acids were 16:0, 18:0, 18:1n-9, 18:1n-7, 20:4n-6, 20:5n-3, and 22:5n-3. The pH levels and total nitrogen and amino nitrogen contents of the hot-water extract (WE) and scrap enzyme hydrolysate (SE) samples from the SA were 6.32 and 6.05, 1.36% and 1.52%, and 342.1 and 403.1 mg/100 g, respectively. The extraction yields and free amino acid contents from SA were 1,317 and 440 mL/kg, and 8,721.1 and 9,070.7 mg/100 g, respectively, and the concentrations of major components were as follows: arginine, glycine, glutamic acid, alanine and lysine. Additionally, the complex extract (WE+SE) was superior to the traditional extract (WE) in terms of extraction yield, amino-nitrogen content, and organoleptic qualities but not odor.

The change of free amino acid composition during radish Kimchi Fermentation (알타리무우김치 숙성과정중 유리아미노산의 변화)

  • 방양선
    • Journal of the Korean Home Economics Association
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    • v.23 no.4
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    • pp.55-60
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    • 1985
  • This study was carried out to compare the change of free amino acid content in the radish Kimchi added with anchovy pickle sauce with that added with 15% NaCl solution during 30 days fermentation. RESULTS : 1. During the fermentation, the pH of both sample A and B showed the highest values of 7.3 and 7.1, respectively, and then both decreased continually to the lowest value of 4.2 at the 30th day. 2.The salinity in the juice of sample A was higher than that of sample B in all the steps during fermentation, accompanied with a slight decrease of the salinity in both cases with time elapsed. 3. The free amino-type nitrogen content of sample A was nearly duplicated in the final step than in the initial one,while that of sample B showed no significant changes in all the processing period. 4. The free amino acids detected in the both samples were aspartic acid, threonine, serine, proline, glutamic acid, glycine, alanine, tyrosine, cysteine, valine, methinonine, isoleucine, leucine, phenylalanine, $\gamma-aminobutyrix acid(\ulcorner), ornithine, lysine, histidine and arginine. 5. The amounts of proline, arginine were the highest in all free amino acids during fermentation, and tasty components of radish Kimchi seemed to relate to glutamic acid, alanine, isoleucine, leucine, phenylalanine, more deliciousness of Kimchi A and B seemed to derive from amino acids of anchovy pickle sauce added to Kimci A, such as alanine, valine, phenylalanine, lysine.

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Manufactureing Conditions and Quality of Dried Meat on the Snow Crab II. Change of Weight Loss Yield on the Steaming and Various Drying Method (건조게육의 가공조건과 품질 II. 증자 및 건조방법에 따른 감량 수율 및 유리 아미노산의 변화)

  • 양철영
    • The Korean Journal of Food And Nutrition
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    • v.12 no.3
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    • pp.258-264
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    • 1999
  • This study was carried out to investigate the status in weight loss yield of edible meat quality and free amino acid contents of the snow crab(Chionoectes japonicus) by steaming and various drying treat-ment. Change of body weight loss in steaming treatment sample were 20.48∼26.72% and yield of edible meat of steaming sample was higher than raw sample(p<0.05) The pH value was increased with steam-ing and drying and pH of trunk meat was more increased than leg meat. Volatile basic nitrogen(VBN) content of vacuum dried sample was highest and VBN of hot-air and freezing dried sample were about 10mg%. Content of vaccum dried sample wre 41.33 and 48,56mg%. Thirty kind of free amino acids dectected in the snow crab and changes of free amino acid in leg meat were markedly reduced by vacuum drying and the reducing ratio of leg meat by freeze drying was little. The major free amino acid of leg and trunk meat were 39.94% 46.78% respectively. The formation of flavor free amino acid in freezing dried sample were high but its hot-air aried sample were small.

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Changes in Fatty Acid Composition and Sensory Characteristics of Cheonggukjang by Addition of Walnut (호두 첨가가 청국장의 지방산 조성 및 관능적 특성에 미치는 영향)

  • Park, Hwa-Young;Choi, Hee-Eun;Jo, Young-In;Choi, Ung-Kyu
    • The Korean Journal of Food And Nutrition
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    • v.29 no.5
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    • pp.628-634
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    • 2016
  • The aim of this study was to determine the changes in fatty acid composition and sensory characteristics of cheonggukjang containing walnuts at 0, 10, 20, or 30%. Amino nitrogen content was decreased with increasing content of walnuts. However, cheonggukjang containing 30% of walnuts met the standard of food sanitation act. Volatile basic nitrogen contents of cheonggukjang containing walnuts was lower than that of cheonggukjang containing no walnut. There was no significant difference in total amino acid content for cheonggukjang containing 0%, 10%, or 20% of walnuts. However, total amino acid content in cheonggukjang containing 30% of walnuts was significantly lower than that in cheonggukjang containing 0%, 10%, or 20% of walnuts. The content of essential amino acid in cheonggukjang containing 30% of walnuts was also significantly lower than that in cheonggukjang containing 0%, 10%, or 20% of walnuts. Saturated fatty acid content was decreased significantly from 16.7% to 3.6% when the content of was increased from 0% to 30%. Total unsaturated fatty acid contents was increased with increasing amounts of walnut added to cheonggukjang. Result of sensory evaluation revealed that the taste and overall quality of cheonggukjang made with 10~20% walnuts were significantly higher compared to those of ordinary cheonggukjang. These results suggest that cheonggukjang made with 10~20% walnuts could be developed as a novel fermented food.

Quality Characteristics of the Kochujang Prepared with Mixture of Meju and Koji during Fermentation (메주와 고오지를 혼용하여 담금한 고추장 숙성중의 품질특성)

  • Choi, Jin-Young;Lee, Taik-Soo;Noh, Bong-Soo
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.125-131
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    • 2000
  • Quality characteristics of kochujang prepared with meju, koji and mixture of the two(meju+koji) were investigated during fermentation to improve quality of kochujang. During fermentation of kochujang, moisture content was in the range of $53.4{\sim}66.5%$, salt was $8.3{\sim}10.1%$, crude protein was $8.3{\sim}19.3%$ and pH of kochujang was $4.6{\sim}5.4$. Amino-nitrogen content increased during fermentation and the levels were $230{\sim}270\;mg%$ after 150 days of fermentation. The highest amino-nitrogen content was found in mixed kochujang at the beginning stage of fermentation, but in koji kochujang after 30 days of fermentation. Maximum reducing sugar content was $15.0{\sim}19.5%$ at 60th day of fermentation. The highest amino acid content of kochujang protein was found in meju kochujang followed by koji kochujang at the first stage of fermentation. The content of glutamic acid, a major amino acid was $1.38{\sim}3.66%$ of total amino acid content. High levels of aspartic acid, leucine, arginine, alanine and phenylalanine were found in the kochujangs. Mixed kochujang showed the highest L value among the samples until 30 days of fermentation. After that, the highest L value was found in meju kochujang. The highest degree of redness was observed in meju kochujang until 60 days of fermentation and in koji kochujang during $90{\sim}120$ days of fermentation.

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Studies on the chages in Free Amono Acids of Yellow Corvenia(Pseudosciaena manchurice) during Gulbi processing (굴비제조중 유리아미노산의 변화에 관한 연구)

  • Na, An-Hee;Shin, Mal-Shick;Jhon, Deok-Young;Hong, Youn-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.3
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    • pp.263-275
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    • 1986
  • Gulbi were processed by salting Yellow corvenia (Pseudosciaena manchurica) with in three ways: the dry salting method with bar salt, the dry salting method with purified salt or with the abdominal brine injection method with purified salt. The half of the sample was dried by the control system of temperature and humidity; the other part was dried by the natural condition. The moisture content of fresh Yellow corvenia muscle and eggs were 76.8%, and 68.2% while those of dried samples decreased to 57.7% and 45.3%, respectively. The total nitrogen content of fresh muscle and eggs were 11.0g% and 7.6g%, respectively (dry weight basis), which decreased slightly during salting and showed no significant changes during drying prosess. The protein nitrogen content of fresh muscle and eggs were 10.2g% and 7.5g%, which decreased during Gulbi processing. On the other hand, the nonprotein nitrogen content of both muscle and eggs increased. The content of free amino acids of fresh muscle and eggs were 508.8mg/100g and 1,110.6mg/100g, which increased to between 5.3 and 2.7 times, respectively after 25 days of drying. The composition patterns of free amino acids in muscle and eggs were similar to each other. The four amino acids - Ala, Glu, Lys and Leu - were most abundant in both fresh and dried samples. These amino acids are known as taste and flavour constituents.

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Effects of Nitrogen and Sulfur Application on Yield and contents of Amino Acids Containing Sulfur of Rice (질소(窒素) 및 유황시용(硫黃施用)이 수도(水稻)의 수량(收量)과 함유황(含硫黃)아미노산(酸) 조성(組成)에 미치는 영향(影響))

  • Kim, Bok-Jin;Back, Jun-Ho;Choi, Hyuk
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.122-128
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    • 1997
  • A pot experiment was carried out using Seomjin-byeo and Samgang-byeo to investigate the effects of nitrogen and sulfur on the growth, yields, and sulfur containing amino acid content of rice. The yields and number of tillers at 50 days after transplanting were increased with the increase of the amounts of nitrogen and sulfur, but N/S ratio was decreased in both varieties. There were a positive correlations between yield and contents of nitrogen and sulfur in straw and brown rice, but a negative correlation between N/S ratio and yields at harvesting stage. Crude protein contents increased with the increase of amounts of nitrogen and sulfur. contents of amino acid containing sulfur in brown rice were positively correlated with the sulfur and nitrogen contents in rice straw.

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Nitrogen Uptake and Growth of Soybean Seedlings under Flooding Stress

  • Won Jun-Yeon;Ji Hee-Chung;Cho Jin-Woong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.2
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    • pp.118-122
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    • 2006
  • This experiment was carried out on plastic pots ($40cm{\times}25cm{\times}30cm$) filled with sand soil at greenhouse using two soybean cultivars with small seed; one was Pungsannamulkong (PSNK) recognized as a tolerant cultivar against excessive water stress and the other one was Sobaeknamulkong (SBNK) recognized as a susceptible cultivar. Seed was sown with 30 plants of 2 hills, and the amount of applied fertilizer was N; 3.0 g, P; 3.0 g, and K; 3.4 g per $m^2$ with all basal fertilizations. Plants were grown under photoperiod of natural light with day temperature of $31{\pm}5^{\circ}C$ and night temperature of $22{\pm}1^{\circ}C$. The flooding treatment was done for 3, 5, 7 and 10 days by filling pots with tap water up to 1 cm above the level of the soil surface when plants were 2 days after emerging. Nitrogen uptake by leaves of soybeans decreased significantly by the flooding after 6 days. This significant reduction of N uptake by flooding was evidently recognized from the chlorosis of leaves. The dry matter of flooded soybean seedlings significantly decreased compared to non-flooded soybean seedlings at 10 days. The dry matter of roots also showed similar result of the shoot. Shoots had more N reduction than roots under the flooding. This N reduction was more pronounce in SBNK than in PSNK. Chlorophyll content of flooded soybeans showed decreasing or non-increasing tendency, and the reduction of chlorophyll content was more in SBNK than in PSNK from the flooding stress. Nitrate content of soybean seedlings with flooding stress showed decreasing tendency in shoot and root parts. Ammonium content, however, was higher in flooding stress compared to the non-flooding. Flooding caused a remarkable change in the AA (amino acid) composition and TAA (total amino acid) concentration in the leaves of soybean seedlings.

Development of Lentil Cheonggukjang Fermented by Bacillus subtilis Isolated from Traditional Soy Sauce (재래간장으로부터 분리한 Bacillus subtilis를 이용하여 제조한 렌틸콩 청국장의 개발)

  • Lee, Yeon Jeong;Kim, Na Young;Kim, Up Sik;Han, Myung Joo
    • Journal of the Korean Society of Food Culture
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    • v.32 no.6
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    • pp.566-575
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    • 2017
  • This study was conducted to screen for superior strains for preparation of Cheonggukjang and identify the optimal fermentation time based on fermentation of lentils by various Bacillus subtilis strains. Bacillus count was significantly high at 48 hrs of fermentation (9.22-9.51 log CFU/g). In addition, the pH was significantly high in the range of 7.60-7.92 at 48 hrs fermentation, and the reducing sugar content was significantly high (0.89-1.45). The amino-type nitrogen content and ammonia-type nitrogen content were significantly increased with fermentation time. Additionally, the amylase activity was significantly high (3.05-4.22) at 48 hrs of fermentation, as was the protease activity (4.80-5.63). Bacillus subtilis S3 (5.63), S4 (5.60) were higher than S1 (4.80), S2 (4.92). The viscous substances and DPPH radical scavenging activity increased at 48 hrs, then decreased. Sensory evaluation revealed that lentil Cheonggukjang fermented by S3 showed the highest scores in color, smell, savory taste, sweet taste, viscous substance and overall acceptability. Therefore, 48 hrs of fermentation should be suitable for lentil Cheonggukjang fermented by Bacillus subtilis S3.