• Title/Summary/Keyword: contaminated water

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Bioremediation of metal contamination groundwater by engineered yeasts expressing phytochelatin synthase (Phytochelatin synthase 발현을 통한 효모의 중금속 처리에 관한 연구)

  • ;;;Wilfred Chen
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.09a
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    • pp.290-292
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    • 2004
  • Heavy metal contamination has been increased in aqueous environments near many industrial facilities, such as metal plating facilities, mining operations, and tanneries. The soils in the vicinity of many military bases are also reported to be contaminated and pose a risk of groundwater and surface water contamination with heavy metals. The biological removal of metals through bioaccumulation has distinct advantages over conventional methods; the process rarely produces undesirable or deleterious chemical byproducts, it is highly efficient, easy to operate and cost-effective in the treatment of large volumes of wastewater containing toxic heavy metals. In addition, a recent development of molecular biology shed light on the enhancing the microorganism's natural remediation capability as well as improving the current biological treatment. In this study, characteristics of the cell growth and heavy metal accumulation by Saccharomyces cerevisiae strains expressing phytochelatin syntahse (PCS) gene were studied in batch cultures. The AtCRFI gene was demonstrated to confer substantial increases in metal tolerance in yeast. PCS-expressing cells tolerated more Cd$^{2+}$ than controls.

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Modality of Heavy Metal Contamination of Soil in Military Rifle Shooting Range (소화기 사격장의 중금속 오염 양상)

  • Lee, Kwang-Lyeol;Hyun, Jae-Hyuk
    • Journal of Soil and Groundwater Environment
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    • v.21 no.3
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    • pp.58-63
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    • 2016
  • The study examined the level of heavy metal contamination by dividing military rifle shooting ranges into the three areas, i.e firing, trajectory, and target. The target area was found to be contaminated at a level higher (Cu 845 mg/kg, Pb 30,487 mg/kg) than the Worrisome Level of Soil Contamination (hereinafter referred to as the “Worrisome Level”) The trajectory area was predicted to be free from contamination, but it did indicate contamination although it was pretty much lower (Cu 23 mg/kg, Pb 99 mg/kg) than Worrisome Level. This is attributed to the contamination spread when rearranging the soil of the target area during the maintenance of the shooting range. The firing area was also predicted to be free from heavy metal contamination, but the results analyzed indicated a contamination higher (Cu 201 mg/kg, Pb 2,286 mg/kg) than Worrisome Level. This is attributed to the fragments of the broken bullet scattering due to the pressure generated as the bullet leaves the muzzle. An examination of heavy metal contamination in the discharge area as well as gutters to prevent the intrusion of rain water from perimeter revealed a high level of contamination (Cu 298 mg/kg, Pb 6,497 mg/kg), which makes it necessary to take measures.

An Electrochemical Study on the Corrosion Property of Materials for Sea Water Heat Exchange System (해수 열교환기용 재료의 부식특성에 관한 전기 화학적 연구)

  • 김진경;김강희;김성종;박근현;문경만
    • Journal of Advanced Marine Engineering and Technology
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    • v.26 no.1
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    • pp.99-107
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    • 2002
  • Recently all kinds of structural materials are subjected to the severe corrosive environment. Especially corrosion problems of heat exchanger such as galvanic corrosion, erosion and cavitation raised by both contaminated solution and high velocity of fluid to increase cooling effect of heat exchanger have been frequently reported in these days. In this study two kinds of sheet materials and five kinds of tube materials are used for galvanic corrosion characteristics and their corrosion current density calculation. The tube materials having the most galvanic corrosion resistance between tube and sheet of heat exchanger were Al Brass(68700) and Al Brass(C6872TS) and although Ti tube predominantly indicated the highest individual corrosion resistance among those five tube materials. it appeared that Ti tube can be allowed as sheet materials to get galvanic corrosion easily. However it is considered that Cu-Ni tube materials is not only easy to produce galvanic corrosion significantly between tube and sheet regardless of kinds of sheet materials but also is appeared considerably its own high corrosion current density

Rapid Quantification of Salmonella in Seafood Using Real-Time PCR Assay

  • Kumar, Rakesh;Surendran, P.K.;Thampuran, Nirmala
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.569-573
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    • 2010
  • A quantitative detection method for Salmonella in seafood was developed using a SYBR Green-based real-time PCR assay. The assay was developed using pure Salmonella DNA at different dilution levels [i.e., 1,000 to 2 genome equivalents (GE)]. The sensitivity of the real-time assay for Salmonella in seeded seafood samples was determined, and the minimum detection level was 20 CFU/g, whereas a detection level of 2 CFU/ml was obtained for pure culture in water with an efficiency of ${\geq}85%$. The real-time assay was evaluated in repeated experiments with seeded seafood samples and the regression coefficient ($R^2$) values were calculated. The performance of the real-time assay was further assessed with naturally contaminated seafood samples, where 4 out of 9 seafood samples tested positive for Salmonella and harbored cells <100 GE/g, which were not detected by direct plating on Salmonella Chromagar media. Thus, the method developed here will be useful for the rapid quantification of Salmonella in seafood, as the assay can be completed within 2-3 h. In addition, with the ability to detect a low number of Salmonella cells in seafood, this proposed method can be used to generate quantitative data on Salmonella in seafood, facilitating the implementation of control measures for Salmonella contamination in seafood at harvest and post-harvest levels.

Heavy Metal Contamination and Process for Its Removal in the Vicinity of the Dalsung Cu-W Mine (달성광산(達成鑛山) 주변지역(周邊地域)에서의 중금속오염(重金屬汚染)과 이의 제거방안(除去方案))

  • Kim, Kyoung-Woong;Hong, Young-Kook;Kim, Taik-Nam
    • Economic and Environmental Geology
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    • v.29 no.1
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    • pp.57-63
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    • 1996
  • The Dalsung copper-tungsten mine in the Taegu area, Korea was closed in 1975 and may be the sources of the heavy metal contamination in the tributary system and soil-plant system due to the mine drainage derived from the mine wastes and tailings. To examine the degree and extent of heavy metal contamination in the vicinity of Dalsung mine area, stream water and soil samples were taken and analyzed for heavy metals by ICP-AES and AAS. Highly contaminated soils are found near the Lower Tunnel No.0 ranging up to $1760{\mu}g/g$ As, $2060{\mu}g/g$ Cu, $1120{\mu}g/g$ Pb and 346 ${\mu}g/g$ Zn. From the results of the sequential extraction methods for the metal speciation, the heavy metals in soils may be derived from soil parent materials and acid mine drainage. With the processes for the heavy metal removal, most of the heavy metal ions in the acid mine drainage are removed by being exchanged with Ca ions held by the bentonite, hydroxyapatite and calcium hydoxide.

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The Development of Real-Time Leak Monitoring System for Management of Hazardous Material Pipeline (유해물질 이송관로 파손누출 실시간 예방 및 감시 기술개발)

  • Chae, Sookwon;Seo, Jaesoon;Kim, Joonseok
    • Journal of the Society of Disaster Information
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    • v.12 no.2
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    • pp.122-129
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    • 2016
  • In this study, a dual structured pipe for a preventive monitoring on a accident damage, a smartphone app program for a survey and a construction data collection and a server program for a real-time monitoring were developed. A pilot system was installed in the test field to analyze effects of a system developed in this study. The data for a damage prevention was detected by the attached sensors on the pipe. Exclusion was tested by the pressure sensors to be installed at regular intervals. The app and server programs was enabled to the real-time data collection and real-time monitoring linked by VRS survey equipments and a smartphone.

Study on Radioactive Contamination of Plant Nearby Nuclear Power Plant - Focused on Pinus thunbergii Parl. and Viburnum awabuki K. KOCH - (원전주변 지역 식물의 방사능 오탁에 관한 연구 - 해송과 아왜나무를 대상으로 -)

  • Kang, Tai-Ho;Zhao, Hong-Xia;Jeong, Jin-Wook;Kook, Seong-Do
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.16 no.3
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    • pp.55-62
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    • 2013
  • Generally, the radioactivity from NPP(Nuclear Power Plants) operation can be released below 3% of DRLs(Derived Release Limits) to environment. It was tried to understand which plant was efficient for absorbing radioactivity in this study. Pinus thunbergii Parl. and Viburnum awabuki K. KOCH were analyzed for radioisotope absorption. The samples were collected at three different locations depending on the distance from NPP at the vicinity 10km away, and 30km away. Gamma radionuclide was not detected from the samples, which means that the direct transition into the plant was not significant. Meanwhile, the very low level of radioactive tritium was detected in the samples. One remark was that every plant has different ability for tritium absorption. These results are expected to be applied to propagation and transplanting in radioactively contaminated area or reducing radioactivity in the soil and water near the plants.

Chinese buffer material for high-level radiawaste disposal --Basic features of GMZ-l

  • WEN Zhijian
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.11b
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    • pp.236-244
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    • 2005
  • Radioactive wastes arising from a wide range of human activities are in many different physical and chemical forms, contaminated with varying radioactivity. Their common feature is the potential hazard associated with their radioactivity and the need to manage them in such a way as to protect the human environment. The geological disposal is regarded as the most reasonable and effective way to safely disposal high-level radioactive wastes in the world. The conceptual model of geological disposal in China is based on a multi-barrier system that combines an isolating geological environment with an engineered barrier system. The buffer is one of the main engineered barriers for HLW repository. The buffer material is expected to maintain its low water permeability, self-sealing property, radio nuclides adsorption and retardation property, thermal conductivity, chemical buffering property, overpack supporting property, stress buffering property over a long period of time. Benotite is selected as the main content of buffer material that can satisfy above. GMZ deposit is selected as the candidate supplier for Chinese buffer material of High Level Radioactive waste repository. This paper presents geological features of GMZ deposit and basic property of GMZ Na bentonite. GMZ bentonite deposit is a super large scale deposits with high content of Montmorillonite (about $75\%$) and GMZ-l, which is Na-bentonite produced from GMZ deposit is selected as reference material for Chinese buffer material study.

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대기압의 변화에 따른 휘발성 오염물질의 토양에서 대기로의 거동

  • Choi Ji-Won;Smith James A.;Hwang Gyeong-Yeop
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2005.04a
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    • pp.114-116
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    • 2005
  • Natural attenuation has been actively studied and often selected as final clean-up process in remediation of contaminated ground-water and soil for the last decade. Accordingly, understanding of natural processes affecting the fate and transport of contaminants in the subsurface becomes important for a success of implementation of the natural remediation strategy, Contaminant advection and diffusion processes in the unsaturated zone are naturally related to environmental changes in the atmosphere. The atmospheric pressure changes affecting the transport of contaminants in the subsurface are investigated in this study. Moisture content, trichloroethylene (TCE) concentration, temperature, and pressure variations in the subsurface were measured for the July, August, November, and December 2001 at Picatinny Arsenal, New Jersey. These data were used for a one-phase flow and one-component transport model in simulating the soil-gas flow and accordingly the TCE transport in the subsurface in accordance with the atmosphere pressure variations at the surface. The soil-gas velocities during the sampling periods varied with a magnitude of $10^{-6}\;to\;10^{-7}\;m\;s^{-1}$ at land surface. The TCE advection fluxes at land surface were several orders of magnitude smaller than the TCE diffusion fluxes. A sensitivy analysis indicated that advection fluxes were more sensitive to changes in geo-environmental conditions compared to diffusion fluxes. Of all the parameters investigated in this study, moisture content has the most significant effect on TCE advection and diffusion fluxes.

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Soil flushing of Pesticide-Contaminated Soil (Soil flushing 기법을 이용한 농약 오염토양 정화)

  • 전민하;최상일;장윤영
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1999.10a
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    • pp.87-89
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    • 1999
  • 본 연구에서는 Sorfactant/Cosolvent 혼합용액을 적용한 Soil flushing 기법에 의해 농약(Endosulfan(6,7,8,9,10,10-Hexachlor-1,5,5a,6,9,9a-hexahydro-6,9-methane-2,3,4benzo (e)dioxathiepin-3-oxide))으로 오염된 토양의 정화효율을 알아보았으며, 회분식 및 연속식 실험을 통하여 최적의 운전조건을 도출하고자 하였다. 세척용액의 적정 사용조건을 알아보기 위한 회분식 실험은 Jar tester를 사용하여 진탕비 (토양 중량 : 세척용액 부피), Surfactant(SDS + POE$_{5}$, POE$_{9}$ + POE$_{14}$, POE$_{5}$ + POE$_{14}$, POE$_{14}$)와 보조용매(water, ethanol, methanol, ethanol+methanol)의 혼합비 및 농도 조건을 변화시켜가며 토양세척을 수행하였다. 세척용액은 보조용매에 Surfactant의 농도를 0.5%, 1%로 용해하여 적용하였다. 연속식 실험은 회분식 실험에서 얻어진 최적 세척용액 사용조건 즉, 계면활성제 SDS + POE$_{5}$(1:1, 용액농도 1%), 보조용매 ethanol을 일정 비율로 혼합한 세척용액을 오염된 토양이 충진된 유리칼럼에 여러 유량조건에서 1 - 20 pore volume까지 통과시켜 각 통과된 pore volume에서의 토양세척 효율을 알아보았다. 본 실험조건에서 얻어진 세척용액의 최적 통과 속도는 0.31 ㎤$cm^{-2}$$min^{-1}$ 이었으며, 세척온도의 증가에 따른 세척효율의 향상은 2$0^{\circ}C$이상에서 크게 둔화되었다. 또한 보조용매의 사용량을 줄이기 위해 에탄올을 물로 1:3까지 희석한 결과 세척효율에 큰 영향이 없음을 알 수 있었다.

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