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The effect of contamination on bonding of orthodontic brackets with a self-etching prirneriadhesive (Self-etching primer/adhesive를 사용한 교정용 브라켓의 접착시 오염이 전단결합강도에 미치는 영향)

  • Kim, Yu-Shin;Lee, Hyung-Soon;Lee, Hyun-Jung;Jeon, Young-Mi;Kim, Jong-Ghee
    • The korean journal of orthodontics
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    • v.34 no.5 s.106
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    • pp.439-447
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    • 2004
  • The purpose of this study was to investigate the influence of water, saliva and blood contamination on the bonding strength of metal brackets with a self-etching primer/adhesive to enamel. Ninety-six extracted human teeth were divided into four groups. The brackets were bonded to enamel with a self- etching primer (3M/Unitek Dental Products. Monorovia California) according to one of four protocols. The teeth were bonded in a dry condition (group D) or in contamination with distilled water (group W), artificial saliva (group S). or fresh human blood (group B) Shear bond strengths were tested using an Instron Universal testing machine. After debonding. bracket and tooth surfaces were examined with a stereomicroscope. In each group, four samples were selected and examined with a Scanning electron microscope of the prepared enamel surface and resin-enamel interlace. The results obtained were summarized as follows: Shear bond Strength if group D $(15.22{\pm}2.86MPa)$ and W $(15.20{\pm}3.85 MPa)$ Were higher than in group B$(12.56{\pm}2.94MPa)$ (p<0.05). There were no statistical differences in the shear bond strengths between groups D. W and S (p>0.05). There was a tendency to have less residual adhesive remaining on the enamel surfaces of group B than group D. The SEW morphology of group D and W showed a more roughened etching pattern than group S and B. Water or saliva contamination on bending of orthodontic brackets with Transbond plus self etching primer had almost no influence on bond strength In this study, the blood contaminated group showed the lowest bond strength, but it was above the clinically acceptable bond strength (5.9-7.8 MPa, Reynold, 1975). The results of this study suggest that acceptable clinical bond strengths can be obtained in wet conditions when self-etching adhesives are used.

Oral Cleft Risk Factors in Rural Area of Indonesia(Sintang) (인도네시아 농촌지역의 구순구개열 위험요인 사례조사)

  • Park, Dae-jin;Lim, Young-soo;Oh, Jee-young;Koh, Kwang-Wook;Song, Sung-Eun;Jo, Eun-Joo
    • Journal of agricultural medicine and community health
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    • v.31 no.2
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    • pp.187-208
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    • 2006
  • Objectives: This study was carried out to evaluate the risk factors of Oral cleft and to inspect the living environments of the rural areas of Sintang, Indonesia Methods: During 3 to 9 August 2004, A questionnaire survey was done for the risk factors of oral cleft. Case group was composed of 11 oral cleft patients who admitted Missionary Hospital whose mother's bloods were analyzed for anemia and hyperlipidemia. Control group was composed of 56 reproductive rural women recruited from near rural villages. Also we surveyed 4 rural areas of Indonesia with simple water test kits. $x^2-test$ for significant difference was analysed. Results: Drinking water was statistically significant risk factor(p<0.05) of oral cleft. Other factors had no statistical significancy. The kind of drinking water was river-originated water. In rural villages, water sanitation state, even boiled water, was very poor. Although $NO_2-N$, $NO_3-N$ was negative, E. coli-form microorganisms were strongly positive in most samples. Total food intake amount was not enough, and vitamin supplements were also under the need. Conclusions: Drinking the contaminated river-water around pregnancy was supposed to be one of the risk factors of oral cleft in Indonesia. Further study is needed for nitrate and mercury.

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Simultaneous Analysis of 13 Pesticides in Groundwater and Evaluation of its Persistent Characteristics

  • Song, Dahee;Park, Sunhwa;Jeon, Sang-Ho;Kim, Ki-In;Hwang, Jong Yeon;Kim, Moonsu;Jo, Hun-Je;Kim, Deok-hyun;Lee, Gyeong-Mi;Kim, Hye-Jin;Kim, Tae-Seung;Chung, Hyen Mi;Kim, Hyun-Koo
    • Korean Journal of Soil Science and Fertilizer
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    • v.50 no.5
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    • pp.434-451
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    • 2017
  • For this study, groundwater samples for 3 years from 2011 through 2013 were collected at 106 groundwater monitoring site in Korea. These groundwater samples were analyzed for 13 pesticides such as cabofuran, pentachlorobenzene, hexachlorobenzene, simazine, atrazine, lindane (gamma-HCH), alachlor, heptachlor, chlordane (total), endosulfan (1, 2), dieldrin, endrin, 4,4-DDT. The objectives of this study were to determine the detection frequency and their concentrations of 13 pesticides and evaluate the health risk level considering ingestion, inhalation, and skin contact using concentrations of 13 pesticides in groundwater samples. An analysis was used for the simultaneous determination for 13 pesticides using GC-MS. GC-MS was performed on HP-5ms, using helium ($1ml\;min^{-1}$) as carrier gas. The average recoveries of the pesticides were from 92.8% to 120.8%. The limits of detection (LODs) were between $0.004{\mu}g\;L^{-1}$ and $0.118{\mu}g\;L^{-1}$ and the limits of quantification (LOQs) were between $0.012{\mu}g\;L^{-1}$ and $0.354{\mu}g\;L^{-1}$. 106 groundwater wells were selected. 54 wells were from well to monitor background groundwater quality and 52 wells were from well to monitor groundwater quality in industrial or contamination source area. Eight pesticides including pentachlorobenzene, lindane (Gamma-HCH), heptachlor, chlordane (total), Endosulfan (1, 2), dieldrin, endrin, and 4,4-DDT were not detected in groundwater samples. The detection frequency for hexachlorobenzene, alachlor, carbofuran and simazine was 23.4%, 11.4%, 7.3%, and 1.0%, respectively. Atrazine was detected once in 2011. The average concentrations were $0.00423{\mu}g\;L^{-1}$ for carbofuran, $0.000243{\mu}g\;L^{-1}$ for alachlor, $0.00015{\mu}g\;L^{-1}$ for simazine, and $0.00001{\mu}g\;L^{-1}$ for hexachlorobenzene. The detection frequency of hexachlorobenzene was high, but the average concentration was low. In the contaminated groundwater, the detection frequency for hexachlorobenzene, alachlor, carbofuran, simazine and atrazine was 26.1%, 21.3%, 7.1%, 1.9% and 0.3%, respectively. In the uncontaminated groundwater, detection frequency for hexachlorobenzene, carbofuran and alachlor were 20.2%, 7.5%, and 1.9% respectively. Simazine and atrazine were not detected at uncontaminated groundwater wells. According to the purpose of groundwater use, atrazine was detected for agricultural groundwater use. Hexachlorobenzene showed high detection frequency at agricultural groundwater use area where the animal feeding area and golf course area were located. Alachlor showed more than 50% detection frequency at cropping area, pollution concern river area, and golf course area. Atrazine was detected in agricultural water use area. By land use, the maximum detection frequency of alachlor was found near an orchard. For human risk assessment, the cancer risk for the 5 pesticides was between $10^{-7}$ and $10^{-10}$, while the non-cancer risk (HQ value) was between $10^{-4}$ and $10^{-6}$. For conclusion, these monitoring study needs to continue because of the possibility of groundwater contamination based on various purpose of groundwater use.

A Study on the DC Resistivity Method to Image the Underground Structure Beneath River or Lake Bottom (하저 지반특성 규명을 위한 수상 전기비저항 탐사에 관한 연구)

  • Kim Jung-Ho;Yi Myeong-Jong;Song Yoonho;Choi Seong-Jun;Lee Seoung Kon;Son Jeong-Sul;Chung Seung-Hwan
    • Geophysics and Geophysical Exploration
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    • v.5 no.4
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    • pp.223-235
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    • 2002
  • Since weak Bones or geological lineaments are likely to be eroded, there may develop weak Bones beneath rivers, and a careful evaluation of ground condition is important to construct structures passing through a river. DC resistivity method, however, has seldomly applied to the investigation of water-covered area, possibly because of difficulties in data aquisition and interpretation. The data aquisition having high quality may be the most important factor, and is more difficult than that in land survey, due to the water layer overlying the underground structure to be imaged. Through the numerical modeling and the analysis of a case history, we studied the method of resistivity survey at the water-covered area, starting from the characteristics of measured data, via data acquisition method, to the interpretation method. We unfolded our discussion according to the installed locations of electrodes, ie., floating them on the water surface, and installing them at the water bottom, because the methods of data acquisition and interpretation vary depending on the electrode location. Through this study, we could confirm that the DC resistivity method can provide fairly reasonable subsurface images. It was also shown that installing electrodes at the water bottom can give the subsurface image with much higher resolution than floating them on the water surface. Since the data acquired at the water-covered area have much lower sensitivity to the underground structure than those at the land, and can be contaminated by the higher noise, such as streaming potential, it would be very important to select the acquisition method and electrode array being able to provide the higher signal-to-noise ratio (S/N ratio) data as well as the high resolving power. Some of the modified electrode arrays can provide the data having reasonably high S/N ratio and need not to install remote electrode(s), and thus, they may be suitable to the resistivity survey at the water-covered area.

The Prediction of the Origin of Microbial Contamination in Kimbab and Improvement of Microbiological Safety by Gamma Irradiation (김밥의 미생물 오염원 예측 및 미생물학적 안전성 개선을 위한 감마선 조사 효과)

  • Lee, Na-Young;Jo, Cheol-Hun;Chung, Hun-Jong;Kang, Ho-Jin;Kim, Jae-Kyung;Kim, Hyun-Ju;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.37 no.2
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    • pp.279-286
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    • 2005
  • Microbial contamination origin of Kimbab was determined using nine types of ready-to-use ingredients, three each from animal, seafood, and vegetable sources. Effect of radiation on microbiological safety was also investigated. Total aerobic bacteria were not detected in seasoned beef, ham, and seasoned burdock, whereas 3.50, 5.41, 8.83, and 5.07 log CFU/g were detected in surimi gel, seasoned and blanched spinach, dried laver, and cucumber, respectively. Total aerobic bacterial and mold numbers were 8.73 and 5.08 log CFU/g in prepared Kimbab. Gamma irradiation reduced level of contaminated aerobic bacteria and mold population in Kimbab and its ingredients, Salmonella mutagenicity assay (Ames test) showed Kimbub ingredients irradiated at 10 kGy did not show any mutagenicity. These results indicate ready-to-use kimbab ingredients were mostly responsible for total aerobic bacteria and mold population of Kimbab, and low dose irradiation and low temperature storage ($10^{\circ}C$) effectively ensured microbiological safety of Kimbab and ready-to-use ingredients.

Studies on Fungal Contamination and Mycotoxins of Rice Straw Round Bale Silage (사료용 볏짚 곤포사일리지의 곰팡이 및 Mycotoxin 오염 연구)

  • Sung, Ha-Guyn;Lee, Joung-Kyong;Seo, Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.4
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    • pp.451-462
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    • 2011
  • The purpose of this study was to investigate fungi and mycotoxin contamination of the rice straw bale silage in Korean. It was tested the 33 samples of rice straw round bale silage with various condition which fed cattle in the farm. The level of fungal contamination was $2.1{\times}10^6\;cfu\;g^{-1}$ in the average and $9.2{\times}10^8\;cfu\;g^{-1}$ in the maximum. The fungal contamination was detected in the all of normal samples which good condition of rice straw bale silage. When the fungi was isolate and identify, it was found 28 species and mycotoxin producing fungi were 8 species as following as Aspergillus flavus, Aspergillus fumigatus, Fusarium culmorum, Fusarium verticillioides, Penicillium carneum, Penicillium paneum, Penicillium roqueforti, Penicillium viridicatum. Specially, Penicillium paneum was found 42% of samples and Aspergillus sp. (A. flavus, A. fumigatus) are 21% of samples. In case of mycotoxin contamination, the 42% of samples are detected more than one kind of mycotoxin. Some samples are contaminated three kinds of mycotoxin. This study was not found aflatoxin ($B_1$, $B_2$, $G_1$, $G_2$) and fumonisin ($B_1$, $B_2$), but were detected the contamination of ochratoxin A (1.0~5.8 ug/kg), deoxynivalenol (DON, 156.0~776.7 ug/kg) and zearalenone (ZON, 38.0~750.0 ug/kg). Therefore, the above results show that rice straw round bale silage expose on hazard factors as mycotoxigenic fungi and mycotoxin contamination, and than need more research about mycotoxin in animal feed to protect animal and human healthy.

Isolation and Characteristics of a Phenol-degrading Bacterium, Rhodococcus pyridinovorans P21 (페놀분해세균 Rhodococcus pyridinovorans P21의 분리 및 페놀분해 특성)

  • Cho, Kwang-Sik;Lee, Sang-Mee;Shin, Myung-Jae;Park, Soo-Yun;Lee, Ye-Ram;Jang, Eun-Young;Son, Hong-Joo
    • Journal of Life Science
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    • v.24 no.9
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    • pp.988-994
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    • 2014
  • The effluents of chemical and petroleum industries often contain non-biodegradable aromatic compounds, with phenol being one of the major organic pollutants present among a wide variety of highly toxic organic chemicals. Phenol is toxic upon ingestion, contact, or inhalation, and it is lethal to fish even at concentrations as low as 0.005 ppm. Phenol biodegradation has been studied in detail using bacterial strains. However, these microorganisms suffer from substrate inhibition at high concentrations of phenol, whereby growth is inhibited. A phenol-degrading bacterium, P21, was isolated from oil-contaminated soil. The phenotypic characteristics and a phylogenetic analysis indicated the close relationship of strain P21 to Rhodococcus pyridinovorans. Phenol biodegradation by strain P21 was studied under shaking condition. The optimal conditions for phenol biodegradation by strain P21 were 0.09% $KNO_3$, 0.1% $K_2HPO_4$, 0.3% $NaH_2PO_4$, 0.015% $MgSO_4{\cdot}7H_2O$, 0.001% $FeSO_4{\cdot}7H_2O$, initial pH 9, and $20-30^{\circ}C$, respectively. When 1,000 ppm of phenol was added to the optimal medium, the strain P21 completely degraded it within two days. Rhodococcus pyridinovorans P21 could grow in up to 1,500 ppm of phenol as the sole carbon source in a batch culture, but it could not grow in a medium containing above 2,000 ppm. Moreover, strain P21 could utilize toxic compounds, such as toluene, xylene, and hexane, as a sole carbon source. However, no growth was detected on chloroform.

Analysis on the Growth Environment of Chionanthus retusus Community at the Wansanchielbong in Jeonju (전주 완산칠봉 이팝나무 자생지의 생육환경으로 본 자연유산 가치 분석)

  • Kim, Yeon
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.28 no.4
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    • pp.85-97
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    • 2010
  • This study analyzed the distribution, structure and environmental condition of the vegetation of the Chionanthus retusus Lindly et Paxton community at the Wansanchielbong in the Jeonju city to offer basic data for sustainable conservation and ecological management system. And the results are as follows; 1. The average pH of soil at the community was pH 5.69 and it was slightly higher than the average of forest soil pH of Korea. But if the degree of pH will be down, it will be needed some more fertilization of Calcium. 2. The total average for contents of organism was 4.98%. And the nitrate - nitrogen content(mg/kg) of A, B, C, D quadrat was 20.29%, 28.87%, 7.65%, and 23.3% respectively. And there were good condition except quadrat C which was contaminated by amount of earth and sand. 3. The flora of the Chionanthus retusus Lindly et Paxton community was listed as 60 taxa; 37 families, 50 genera, 47 species, 10 varieties and 3 forms. The average appearance species of each Quadrat were A sector 30, B sector 26, C sector 19 and D 19 taxa respectively. 4. Surveyed woody plants in the community were as follows : Chionanthus retusus, Zelkova serrata, Quercus variabilis, Cornus walteri, Robinia pseudo-acacia and those were mixed status. And Chionanthus retusus, Zelkova serrata, Robinia pseudo-acacia, Albizzia julibrisin, Cudrania tricuspidata, Symplocos chinensis for. pilosa were mixed in mid layer trees. Herbaceous plants were founded such as Chionanthus retusus, Zelkova serrata, Robinia pseudo-acacia, Grewia parviflora, Rosa multiflora, Trachelospermum asiaticum was dominant with 35~64% in the ground cover, and Commelina communis, Calamagrostis arundinacea, Dryopteris bissetiana, Lilium lancifolium were founded also. 5. The importance values of Chionanthus retusus was 40.2% in the quadrat A1, 50.2% at quadrat A, 50.0% B1, 45.2% B2, 22.4% C1, 73.6% C2, 33.2% D1 and the total average of I.V. was 44.9%. 6. The average height of surveyed Chionanthus retusus was 5.7m and the average DBH was 12.4cm. The number of trees higher than 2m were 107 and the number of trees lower than 2m were 63. The total numbers of Chionanthus retusus were 170. 7. The age of surveyed Chionanthus retusus were analyzed 42 thru 87 years old and that of Zelkova serrata were 42, Quercus variabilis were 60, Quercus aliena were 48, Robinia pseudo-acacia were 40. 8. The number of trees with DBH 40 through 50cm were 6, and that of 30~39cm were 3, and that of 20~29cm were 16, so the total number that was over 20cm was 25. And there were 70 trees under 10cm of DBH and 63 seedlings. It will be very important data to conserve the habitat that the structure and environmental condition of the Chionanthus retusus Lindly et Paxton community at the Wansanchielbong was stable, and sustainable monitoring will be needed. Now that community is nurse forest of Jeonju City but more positive preservation plan will be needed and assigning monument of city or province also be necessary.

Case Study of Hydrochemical Contamination by Antimony Waste Disposal in Korea (국내 안티몬폐기물에 의한 수질화학적 오염 사례연구)

  • Jeong, Chan-Ho
    • The Journal of Engineering Geology
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    • v.18 no.4
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    • pp.471-482
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    • 2008
  • This study was carried out to investigate the contamination characteristics of surface water, soil water and groundwater around and in antimony waste landfill site in Wonsung-ri, Yeonki-kun, Chungnam. The waste disposed in the study was excavated and transported to the other site in several years ago. For this study, we collected 35 water samples including groundwater, soil water and surface in the study site and also collected 2 groundwater samples from a comparison site. The data of chemical analysis of soil water samples show the antimony concentration of $48.75{\sim}74.81\;ppb$, which is much higher than groundwater in a comparison site and is highly excess than regulation level for a drinking water of some advanced countries. A relatively high antimony concentration was detected in three stream water samples nearby landfill site and two groundwater samples. Fe and Mn contents in soil water and stream water were measured as maxium 6.5 mg/L and 7.3 mg/L, respectively. Although other heavy metals of water samples in the study site are higher concentration than water sample of comparison site, their absolute levels are lower than regulation level for a drinking water. The chemical data of water samples are plotted widely from Ca - $HCO_3$ type to Ca - ($Cl +SO_4+NO_3$) type. Some groundwater show high contents of potassium and nitrate, which would come from fertilizer and sewage. Conclusively, some heavy metals including antimony have been still remained under the soil surface of the landfill site in the past. These metals have leaked out into nearby stream and groundwater system, and threaten the ecology, crops and the health of residents in this site. Therefore, the government have to prepare the strategy to prevent the diffusion of heavy metals into aquatic environment and have to process the reclamation work for contaminated site. It is also necessary to make a regulation level of the antimony concentration for a drinking water and soil environment in Korea.

Characteristic Assessment of Heavy Metals in Dusts Collected by the Air Filtration System at Subway Stations in Daegu, Korea (대구지역 지하철역사 공기여과필터 포집먼지에 함유된 중금속성분의 특성평가)

  • Do, Hwa-Seok;Song, Hee-Bong;Shin, Dong-Chan;Kwak, Jin-Hee;Lee, Myoung-Sook;Yoon, Ho-Suk;Kang, Hye-Jung;Phee, Young-Gyu
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.1
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    • pp.42-50
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    • 2009
  • Samples of subway dust were collected by the air filter system of 30 subway stations on Daegu subway line 1 in January 2008. Samples were sieved below 100 ${\mu}m$, and 14 elements were analyzed using ICP after acid extraction. Results obtained from the source assessment of trace elements using enrichment factor showed that Ca, Fe, K, Mg, Mn, Na, V were influenced by natural sources such as weathered rock and resuspended soil, while Cd, Cr, Cu, Ni, Pb and Zn were influenced by anthropogenic sources such as fuel combustion and waste incineration. Concentrations were remarkably higher in components from natural sources than in components from anthropogenic sources. Anthropogenic sources were significantly affected by indoor dusts than outdoor dusts. Results of pollution indices of heavy metals indicated that indoor dusts were more contaminated with heavy metal ions than outdoor dusts. The correlation analysis among trace elements indicated that components were much correlated in the order of natural sources-anthropogenic sources, anthropogenic sources-anthropogenic sources, natural sources-natural sources in both indoor and outdoor dusts. Trace element components of outdoor dusts were largely correlated than those of indoor dusts. In addition, indoor dusts were significantly affected by outdoor dusts rather than depth from the surface or the average daily number of subway passengers.