• Title/Summary/Keyword: contact effect

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Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts

  • Sohn, Hae-Jin;Seo, Ga-Eun;Lee, Jae-Ho;Ham, A-Jeong;Oh, Young-Hwan;Kang, Heekyoung;Shin, Ho-Joon
    • Parasites, Hosts and Diseases
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    • v.57 no.3
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    • pp.217-223
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    • 2019
  • Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this study, we observed the cytopathic effect, in vitro cytotoxicity, and secretion pattern of cytokines in human corneal epithelial cells (HCECs) induced by A. castellanii trophozoites and/or cysts. Morphological observation revealed that panked dendritic HCECs co-cultured with amoeba cysts had changed into round shape and gradually died. Such changes were more severe in co-culture with cyst than those of co-cultivation with trophozoites. In vitro cytotoxicity assay revealed the highest cytotoxicity to HCECs in the co-culture system with amoeba cysts. A. castellanii induced the expression of $IL-1{\alpha}$, IL-6, IL-8, and CXCL1 in HCECs. Secreted levels of $IL-1{\alpha}$, IL-6, and IL-8 in HCECs co-cultured with both trophozoites and cysts were increased at an early incubation time (3 and 6 hr). These results suggested that cytopathic changes and pro-inflammatory cytokines release of HCECs in response to A. castellanii, especially amoebic cysts, are an important mechanism for AK development.

Rotordynamic Model Development with Consideration of Rotor Core Laminations for 2.2 kW-Class Squirrel-Cage Type Induction Motors and Influence Investigation of Bearing Clearance (2.2 kW급 유도전동기의 회전자 적층구조를 고려한 회전체 동역학 해석모델 개발 및 베어링 간극의 영향 분석)

  • Park, Jisu;Sim, Kyuho;Lee, Sung-Ho
    • Tribology and Lubricants
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    • v.35 no.3
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    • pp.158-168
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    • 2019
  • This paper presents the investigation of two types of rotordynamic modeling issues for 2.2 kW-class, rated speed of 1,800 rpm, squirrel-cage type induction motors. These issues include the lamination structure of rotor cores, and the radial clearance of ball bearings that support the shaft of the motor. Firstly, we focus on identifying the effects of rotor core lamination on the rotordynamic analysis via a 2D prediction model. The influence of lamination is considered as the change in the elastic modulus of the rotor core, which is determined by a modification factor ranging from 0 to 1.0. The analysis results show that the unbalanced response of the rotor-bearing system significantly varies depending on the value of the modification factor. Through modal testing of the system, the modification factor of 0.079 is proven to be appropriate to consider the effects of lamination. Next, we investigate the influence of ball bearing clearance on the rotordynamic analysis by establishing a bearing analysis model based on Hertz's contact theory. The analysis results indicate that negative clearance greatly changes the bearing static behavior. Rotordynamic analysis using predicted bearing stiffness with various clearances from -0.005 mm to 0.010 mm reveals that variations in clearance result in a slight difference in the displacement of the system up to 18.18. Thus, considering lamination in rotordynamic analysis is necessary as it can cause serious analysis errors in unbalanced response. However, considering the effect of the bearing clearance is optional because of its relatively weak impact.

Effect of Si Doping in Self-Assembled InAs Quantum Dots on Infrared Photodetector Properties (Si 도핑이 InAs 자기조립 양자점 적외선 소자 특성에 미치는 효과)

  • Seo, Dong-Bum;Hwang, Je-hwan;Oh, Boram;Kim, Jun Oh;Lee, Sang Jun;Kim, Eui-Tae
    • Korean Journal of Materials Research
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    • v.29 no.9
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    • pp.542-546
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    • 2019
  • We investigate the characteristics of self-assembled quantum dot infrared photodetectors(QDIPs) based on doping level. Two kinds of QDIP samples are prepared using molecular beam epitaxy : $n^+-i(QD)-n^+$ QDIP with undoped quantum dot(QD) active region and $n^+-n^-(QD)-n^+$ QDIP containing Si direct doped QDs. InAs QDIPs were grown on semi-insulating GaAs (100) wafers by molecular-beam epitaxy. Both top and bottom contact GaAs layer are Si doped at $2{\times}10^{18}/cm^3$. The QD layers are grown by two-monolayer of InAs deposition and capped by InGaAs layer. For the $n^+-n^-(QD)-n^+$ structure, Si dopant is directly doped in InAs QD at $2{\times}10^{17}/cm^3$. Undoped and doped QDIPs show a photoresponse peak at about $8.3{\mu}m$, ranging from $6{\sim}10{\mu}m$ at 10 K. The intensity of the doped QDIP photoresponse is higher than that of the undoped QDIP on same temperature. Undoped QDIP yields a photoresponse of up to 50 K, whereas doped QDIP has a response of up to 30 K only. This result suggests that the doping level of QDs should be appropriately determined by compromising between photoresponsivity and operating temperature.

The Prevention Effect of Seawater Penetration of Gel Coat Applied in Repair of FRP Fishing Vessel (FRP 어선 보수에 적용되는 겔코트의 해수 침투 방지 효과)

  • Kang, Dae Kon;Park, Jai Hak
    • Journal of the Korean Society of Safety
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    • v.33 no.6
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    • pp.15-21
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    • 2018
  • Ships may collide with reefs or other objects during operation, when arriving or departing ports. The hull plate may be damaged due to the contact with other ships. The total number of domestic powered fishing vessels has decreased, but that of FRP fishing vessels has increased by 0.7% and the ratio of FRP fishing vessels to the total fishing vessels increased to 96%. Recently, fishing vessels has been used as fishing boats for income of non-fishermen as well as fishermen. Therefore, safety management for repair and maintenance is necessary. The penetration of moisture and moisture in the composite material such as FRP may deteriorate the mechanical properties and the salt (NaCl) component of the damaged portion may cause a relatively high deterioration in material strength. The gel coat painting is the final stage of repairs ans maintenance of FRP fishing vessels. The thickness criteria in the domestic and foreign gel coat is 0.3~0.762 mm. The joint specimens, which was immersed in seawater for 120 days, were compared with those without seawater immersion. As a result, the tensile strength was 83 ~ 121.8% and the flexural strength was 83 ~ 113% compared with the specimens without seawater immersion. According to the previous study the tensile strength decreased by more than 29% and the flexural strength decreased by more than 50% when the composite material was immersed in seawater for 1,083 hours without coating. As a result, it was found that the gel coat with 0.5 mm thickness is very effective in preventing the strength decrease of the composite material.

Experimental Study on the Wear Effects of a Brush Seal in DN 2.5million in a 250℃ High - temperature Steam Environment (DN 250만 250℃고온 스팀환경에서 운전되는 단열 브러쉬 실 마모효과에 관한 실험적 연구)

  • Ha, YunSeok;Ha, TaeWoong;Lee, YoungBok
    • Tribology and Lubricants
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    • v.35 no.2
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    • pp.99-105
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    • 2019
  • This study presents an experimental investigation of the wear and oxidation of the bristles of a brush seal in a super-heated steam environment. We construct a model reflecting normal force and radial interference to predict the amount of wear. To monitor the volume loss of the bristle induced by the swirl phenomenon of the rotor, we measure the clearance between the rotor and the brush seal by using a non-contact 3-D device. We calculate the area by using the area-wise measurement method. Considering the obvious brush seal wear variables, we use two disks with different roughness($Ra=0.1{\mu}m$ and $100{\mu}m$) to determine the effect of roughness on wear. Considering an actual steam turbine, we utilize a steam generator and super-heater to generate a working fluid (0.95MPa, 523.15K) that has high kinetic energy. We observe the abrasion of the bristles in the hot steam environment through a scanning electron microscope image. This study also conducted energy dispersive X-ray (EDX) analysis for a qualitative evaluation of local chemistry. The results indicate that the wear and elimination of bristles occur on the disk with high roughness, and the weight increases due to oxidation. Furthermore these results, reveal that the bristle oxidation is accelerated more under super-heated steam conditions than under conditions without steam.

Comparison of the removal torque and a histomorphometric evaluation of the RBM treated implants with the RBM followed by laser treated implants: an experimental study in rabbits

  • Park, Eun Young;Sohn, Hae Ok;Kim, Eun-Kyong
    • Journal of Yeungnam Medical Science
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    • v.36 no.1
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    • pp.43-49
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    • 2019
  • Background: In the osseointegration of dental implants, the implant surface properties have been reported to be some of the most important critical factors. The effect of implant's surfaces created by resorbable blast media (RBM) followed by laser ablation on bone tissue reactions was examined using the removal torque test and histomorphometric analysis. Methods: Two types of dental implants, RBM-laser implants (experimental group) and RBM implants (control group) (CSM implant system, Daegu, Korea; L=6 mm, diameter=3.75 mm) were placed into the right and left distal femoral metaphysis of 17 adult rabbits. Six weeks after placement, removal torque was measured and histomorphometric analysis was performed. Results: The mean removal torque was $24.0{\pm}10.2Ncm$ and $46.6{\pm}16.4Ncm$ for the control and test specimens, respectively. The experimental RBM-laser implants had significantly higher removal torque values than the control RBM implants (p=0.013). The mean values of total and cortical bone to implant contact (BIC) were respectively $46.3{\pm}10.8%$ and $65.3{\pm}12.5%$ for the experimental group, and $41.9{\pm}18.5%$ and $57.6{\pm}10.6%$ for the control group. The experimental RBM-laser implants showed a higher degree of total and cortical BIC compared with RBM implants, but there was no statistical significance (p=0.482, 0.225). Conclusion: The removal torque and BIC of the test group were higher than those of the control group. In this study, the surface treatment created by RBM treatment followed by laser ablation appears to have a potential in improving bone tissue reactions of dental implants.

Preparation of Flame Retardant and Antibacterial Wood with Composite Membrane Coating

  • XU, Jun-xian;LIU, Yang;WEN, Ming-yu;PARK, Hee-Jun;ZHU, Jia-zhi;LIU, Yu-nan
    • Journal of the Korean Wood Science and Technology
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    • v.49 no.6
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    • pp.658-666
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    • 2021
  • A novel flame retardant and antibacterial composite membrane coating for wood surfaces was prepared by adding POSS-based phosphorous nitrogen flame retardant (later referred to as NH2-POSS) and silver nanoparticles (Ag NPs) to chitosan (CS). The effects of NH2-POSS content (mass fractions of CS 0%, 0.5%, 1%, 3%, 5%, and 7%) on the structure and properties of the composite membrane coating on wood were investigated. The composite film was prepared by the method of blending and ducting. Contact angle, tensile property and antibacterial effects of the composite film were measured, and infrared spectroscopy was used. The results show that the addition of NH2-POSS can not only improve the toughness of the membrane, but also the flame retardancy of the membrane, which improves the application of the membrane in wood products. However, with the addition of NH2-POSS, the transparency of the composite membrane was weakened. The inhibitory effect of the composite membrane on the growth of Escherichia coli was enhanced with the increase in Ag NPs. This research provides a foundation for the application of functional wood.

Effects of Mg-Al Alloy and Pure Ti on High Temperature Wetting and Coherency on Al Interface Using the Sessile Drop Method (정적법을 이용한 Mg-Al계 합금과 순수 Ti의 고온 젖음현상 및 Al계면에서의 정합성에 미치는 영향)

  • Han, Chang-Suk;Kim, Woo-Suk
    • Korean Journal of Materials Research
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    • v.31 no.1
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    • pp.38-42
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    • 2021
  • In this study, high temperature wetting analysis and AZ80/Ti interfacial structure observation are performed for the mixture of AZ80 and Ti, and the effect of Al on wetting in Mg alloy is examined. Both molten AZ80 and pure Mg have excellent wettability because the wet angle between molten droplets and the Ti substrate is about 10° from initial contact. Wetting angle decreases with time, and wetting phenomenon continues between droplets and substrate; the change in wetting angle does not show a significant difference when comparing AZ80-Ti and Mg-Ti. As a result of XRD of the lower surface of the AZ80-Ti sample, in addition to the Ti peak of the substrate, the peak of TiAl3, which is a Ti-Al intermetallic compound, is confirmed, and TiAl3 is generated in the Al enrichment region of the Ti substrate surface. EDS analysis is performed on the droplet tip portion of the sample section in which pure Mg droplets are dropped on the Ti substrate. Concentration of oxygen by the natural oxide film is not confirmed on the Ti surface, but oxygen is distributed at the tip of the droplet on the Mg side. Molten AZ80 and Ti-based compound phases are produced by thickening of Al in the vicinity of Ti after wetting is completed, and Al in the Mg alloy does not affect the wetting. The driving force of wetting progression is a thermite reaction that occurs between Mg and TiO2, and then Al in AZ80 thickens on the Ti substrate interface to form an intermetallic compound.

Effect of addition of Tl+ and Pd2+ on the texture and hardness of the non-cyanide gold plating layer (논시안 금도금층의 조직과 경도에 미치는 Tl+ 과 Pd2+ 이온첨가의 영향)

  • Heo, Wonyoung;Son, Injoon
    • Journal of Surface Science and Engineering
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    • v.55 no.6
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    • pp.460-468
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    • 2022
  • Due to its high electrical conductivity, low contact resistance, good weldability and high corrosion resi-stance, gold is widely used in electronic components such as connectors and printed circuit boards (PCB). Gold ion salts currently used in gold plating are largely cyan-based salts and non-cyanic salts. The cya-nide bath can be used for both high and low hardness, but the non-cyanide bath can be used for low hardness plating. Potassium gold cyanide (KAu(CN)2) as a cyanide type and sodium gold sulfite (Na3[Au(SO)3]2) salt as a non-cyanide type are most widely used. Although the cyan bath has excellent performance in plating, potassium gold cyanide (KAu(CN)2) used in the cyan bath is classified as a poison and a toxic substance and has strong toxicity, which tends to damage the positive photoresist film and make it difficult to form a straight side-wall. There is a need to supplement this. Therefore, it is intended to supplement this with an eco-friendly process using sodium sulfite sodium salt that does not contain cyan. Therefore, the main goal is to form a gold plating layer with a controllable hardness using a non-cyanide gold plating solution. In this study, the composition of a non-cyanide gold plating solution that maintains hardness even after annealing is generated through gold-palladium alloying by adding thallium, a crystal regulator among electrolysis factors affecting the structure and hardness, and changes in plating layer structure and crystallinity before and after annealing the correlation with the hardness.

The Effect of Group Validation Therapy(V/T) in the Elderly with Dementia (집단인정치료(Group validation therapy)가 치매노인에게 미치는 영향에 관한 연구)

  • Chang, Woo-Shim
    • 한국노년학
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    • v.28 no.4
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    • pp.1023-1039
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    • 2008
  • The purpose of this study was to develop a group validation therapy(V/T) which could be implemented for the elderly with dementia in nursing home, and to evaluate the effectiveness of the program on cognition, ADL(Activity of Daily Living), depression, problematic behavior and QOL(Quality of Life). Subjects were recruited from 4 nursing homes in D city. The sample comprised forty elderly with dementia, capable of verbal communication. Each twenty were in an experimental and control groups. However, four elders with dementia dropped out in experimental and control groups due to personal affairs. Experimental group completed twelve consecutive group validation therapy sessions that combined centering, asking factual questions, rephrasing, identifying and using the preferred sense, asking the extreme, imagining the opposite, reminiscing, touching, maintaining eye contact and a caring tone of voice, observing, matching and expressing the emotion with emotion, using ambiguity, linking behavior with a basic human need, using music and mirroring techniques. Following the intervention, experimental group experienced a significant improvement in cognition, ADL, depression, and QOL. But it is a nonsignificant in problematic behavior on statistically. As a result, a group validation therapy should be applied as an effective and practical psychosocial intervention for the elderly with dementia.