• Asian-Australasian Journal of Animal Sciences
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• v.17 no.10
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• pp.1338-1343
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• 2004

The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was uccessfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.195-201
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• 2008

A simplified technique that cryoprotects zygotic embryos by desiccation was developed for germplasm conservation of wild yam species(Dioscorea spp.) in Korea. Comparative studies with three other cryogenic techniques were conducted. The maximum survival of zygotic embryos were achieved at a frequency of 96.6% when embryos were cryopreserved by the desiccation method. For the successful cryopreservation of yam zygotic embryos, those that were excised from immature/mature seeds were dried in the air stream of a laminar flow cabinet for 30 min at room temperature and then directly immersed in liquid nitrogen.

• BMB Reports
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• v.42 no.12
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• pp.823-828
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• 2009

Techniques for analyzing protein-DNA interactions on a genome-wide scale have recently established regulatory roles for distal enhancers. However, the large sizes of higher eukaryotic genomes have made identification of these elements difficult. Information regarding sequence conservation, exon annotation and repetitive regions can be used to reduce the size of the search region. However, previously developed resources are inadequate for consolidating such information. CONVIRT is a web resource for the identification of transcription factor binding sites and also features comparative genomics. Genomic information on ortholog-independent conserved regions, exons, repeats and sequences is integrated into the virtual chromosome, and statistically over-represented single or combinations of transcription factor binding sites are sought. CONVIRT provides regulatory network analysis for several organisms with long promoter regions and permits inter-species genome alignments. CONVIRT is freely available at http://biosoft.kaist.ac.kr/convirt.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.3
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• pp.86-96
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• 2003

This study aims to improve system for the enlargement of artificial planting spaces through grasp several problems about it and related system in domestic and foreign. In order to improve system for the extension of artificial planting spaces in the urban area following plans have been presented. First, it is necessary to launch various bonus, subsidies and incentives for making planting spaces in the urban area. Second, subdivision of related regulations for using diverse planting methods is needed. Third, government has to take the initiative in pushing on with planting of artificial spaces. Fourth, it is necessary to development of concrete techniques. Finally, it is desirable to plant artificial spaces which animal and plant can live, low effect to environment and material can be circulated although the value of plantation as a meaning of simple green also are admitted. Planting of artificial spaces should be introduced in a positive light for conservation and restoration of urban ecosystem.

• Korea-Australia Rheology Journal
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• v.16 no.1
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• pp.1-15
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• 2004

The computation of viscoelastic flow using neural networks and stochastic simulation (CVFNNSS) is developed from the point of view of Eulerian CONNFFESSIT (calculation of non-Newtonian flows: finite elements and stochastic simulation techniques). The present method is based on the combination of radial basis function networks (RBFNs) and Brownian configuration fields (BCFs) where the stress is computed from an ensemble of continuous configuration fields instead of convecting discrete particles, and the velocity field is determined by solving the conservation equations for mass and momentum with a finite point method based on RBFNs. The method does not require any kind of element-type discretisation of the analysis domain. The method is verified and its capability is demonstrated with the start-up planar Couette flow, the Poiseuille flow and the lid driven cavity flow of Hookean and FENE model materials.

• Proceedings of the KIEE Conference
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• 2005.05a
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• pp.209-211
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• 2005

This paper proposes a adaptive medium-access control(MAC) protocol designed for low-power wireless multi-hop sensor networks which is used for connecting physical world and cyber computing space. Wireless multi-hop sensor networks use battery-operated computing and sensing device. We expect sensor networks to be deployed in an ad hoc fashion, with nodes remaining inactive for long time, but becoming suddenly active when specific event is detected. These characteristics of multi-hop sensor networks and applications motivate a MAC that is different from traditional wireless MACs about power conservation scheme, such as IEEE 802.11. Proposed MAC uses a few techniques to reduce energy consumption. Result show that proposed MAC obtains more energy savings.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.32A no.1
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• pp.61-69
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• 1995

a numerical method to the problem of mutual admittance between two slots is considered by use of conservation of complex power. Calculation of mutual admittance is compared with other results. And mutual admittance obtained by the method is used in the analysis for the broadband rectangular microstrip antenna geometry using E0plane gap coupling. The theoretical results for both return loss and radiation pattern of the broadband antenna are compared with experimental results. Good agreement between calculated and measured values has been observed.

• 한국전산유체공학회:학술대회논문집
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• 2010.05a
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• pp.397-403
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• 2010

The Immersed boundary method(IBM) is one of CFD techniques which can simulate flow field around complex objectives using simple Cartesian grid system. In the previous studies the IBM has mostly been implemented to fractional step method based Navier-Stokes solvers. In these cases, pressure buildup near IB was found to occur when linear interpolation and stadard mass conservation is used and the interpolation scheme became complicated when higher order of interpolation is adopted. In this study, we implement the IBM to an incompressible Navier-Stokes solver which uses SIMPLE algorithm. Bi-linear and quadratic interpolation equations were formulated by using only geometric information of boundary to reconstruct velocities near IB. Flow around 2D circular cylinder at Re=40 and 100 was solved by using these formulations. It was found that the pressure buildup was not observed even when the bi-linear interpolation was adopted. The use of quadratic interpolation made the predicted aerodynamic forces in good agreement with those of previous studies.

• Journal of Korean Society of Rural Planning
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• v.8 no.2 s.16
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• pp.50-56
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• 2002

In 1980s, studies on the river environment and the management techniques to maintain the quality of water achieved actively. From the beginning of 1990s, the concept considering the river ecology and conservation in urban area have been introduced in Korea. Recently, some environment friendly projects on the rivers have been executed and some are under way. Many small rivers in rural areas have been maintained considering landscape and partly ecology. However until the pilot project named Songsam drainage channel project started in 1998, there were no studies and projects on the irrigation and drainage channels considering environment friendly maintenance. Korea Agricultural and Rural Infrastructure Corporation (KARICO) is going to introduce environment friendly and naturally favorable maintenance technique when the new projects to reform the irrigation and drainage channels are planned. Irrigation channel in O-dong project site in Yeomsan-myeon, Younggwang-gun was designed considering environment friendly consolidation and maintenance.

• Proceedings of the Korea Concrete Institute Conference
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• 1996.04a
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• pp.141-145
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• 1996

The primary purpose of this study is to investigate reusal techniques of by-product produced the combined heat power plant in the construction field, which may contribute to the savings of construction materials and the conservation of enviornment. This study is compared and evaluated by testing the chemical resistance, adiabatic temperature rising test, creep and drying shrinkage. As the result of the study, the following conclusions are derived : (1) hydration heat of the fly ash concrete is less than the plain concrete in adiabatic temperature rising test, (2) the fly axh concrete (FA 30%) is similar to the plain concrete in the chemical resistamce, (3) the fly ash concrete (FA 10, 30%) is similar to the plain concrete in drying shrinkage, but the fly ash concrete (FA 50%) is highly increased, (4) the fly ash concrete (FA 30%) is less than the plain concrete in creep test.