• Title/Summary/Keyword: conjugation frequency

Search Result 40, Processing Time 0.025 seconds

Planar Active Rectrodirective Array With Subharmonic Phase Conjugation Mixers

  • Kim Gi-Rae;Park Ji-Yong
    • Journal of information and communication convergence engineering
    • /
    • v.2 no.3
    • /
    • pp.153-156
    • /
    • 2004
  • A planar active retrodirective four-element array with subharmonic phase conjugation mixers based on anti-parallel diode pairs (APDPs) is proposed. As compared to previous phase conjugation mixers using twice RF frequency for LO frequency, the proposed conjugation mixers need only half RF frequency so that it can be easily applied for millimeter-wave applications. Receiving, transmitting, local oscillator, and intermediate frequencies are 5.79, 5.81, 2.9 GHz, and 10 MHz. Monostatic RCS and Bistatic RCS measurements at source locations of $0^{\circ},\;-20^{\circ},\;and\;28^{\circ}$ show good agreement with the calculated data.

The Auto-Tracking Communication Link Using Planar Active Rectrodirective Array

  • Kim, Gi-Rae
    • Journal of information and communication convergence engineering
    • /
    • v.5 no.2
    • /
    • pp.121-125
    • /
    • 2007
  • In this paper, a planar active retrodirective four-element array with subharmonic phase conjugation mixers based on anti-parallel diode pairs (APDPs) is designed, and its application in the auto-tracking duplex communication link is presented. As compared to previous phase conjugation mixers using twice RF frequency for LO frequency, the proposed conjugation mixers need only half RF frequency so that it can be easily applied for millimeter-wave applications. The proposed architecture, which conventionally performs the function of the transmission of an incident signal back in the direction of its source, is modified in order to include a receive function. Experimental verification of these architectures is performed at 1GHz and the results from the prototypes are compared with a theoretical model.

Bit Error Parameters on Passive Phase Conjugation Underwater Acoustic Communication (수동 페이저 컨쥬게이션 수중음향통신 기법의 비트오류 영향 인자)

  • Yoon, Jong-Rak;Park, Moon-Kab;Ro, Yong-Ju
    • The Journal of the Acoustical Society of Korea
    • /
    • v.24 no.8
    • /
    • pp.454-461
    • /
    • 2005
  • Time spread due to the multipath in underwater acoustic channel, induces ISI (Inter-Symbol Interference) which degrades the performance of the underwater acoustic communication system. The passive phase conjugation (PPC) which improves the signal to multipath interference ratio (SMR) and therefore reduces the frequency selectivity. is a diversity communication technique giving a less ISI under multipath fading channel. Its frequency selectivity depends on the number of receiver array sensors and time varying source to receiver range. In this study, frequency selectivity of the PPC and its effects on bit error of underwater acoustic communication is analyzed by numerical simulation.

Analysis of Tetracycline Resistance Plasmids and the Conjugative Transfer of Tetracycline Resistancy in Staphylococcus epidermidis (Staphylococcus epidermidis의 Tetracycline 내성 Plasmid의 분석 및 Conjugation에 의한 내성전달)

  • Chung, Jae-Kyu;Park, Mi-Kyung;Kim, Sung-Kwang
    • The Journal of the Korean Society for Microbiology
    • /
    • v.22 no.1
    • /
    • pp.95-99
    • /
    • 1987
  • When tetracycline resistancy were cured by ethidium bromide treatment, some of the cured strains lost the tetracycline resistance plasmid while other strains kept the plasmids. Both strains of lost and remained plasmids were digested with restriction endonuclease Hind III and these cleaved plasmids were compared with that of parent strains, two plasmid remained strains showed same cleavage patterns between parent and cured strains, however, one plasmid lost strain showed dissimilarity with parent strain, but in the other one strain, among 4 plasmid lost colonies, 2 showed same but other 2 showed different patterns compared to parent strain. Tetracycline was transfered by conjugation in on set(Staphylococcus aureus donor versus Staphylococcus epidermidis, recipient) with relative high frequency but the other 2 sets showed a low degree of frequency and the other 2 sets exhibited no transfer.

  • PDF

Dependence of Optical Phase Conjugation on Incident Beam Position and Intensity (입사빔의 위치 및 세기에 따른 자기펌핑 위상공액파의 특성)

  • 손동환;전병욱;이임걸;손정영;임동건
    • Journal of the Korean Institute of Telematics and Electronics A
    • /
    • v.30A no.3
    • /
    • pp.43-51
    • /
    • 1993
  • Effects of incident beam position and intensity on self-pumped optical phase conjugation are presented using barium titanate as an optical phase conjugator. Depending on the position of incident beam, the crystal used consists of four major operating regions: irregular pulsing, regular pulsing, stable phase conjugation, and unstable oscillating regions. In the second region, the pulsing frequency and amplitude of phase conjugate beam are proportional to I$_{in}$ and I$_{in}^{0.85}$, respectively, where I$_{in}$ is the incident beam intensity. In the fouth region, the rising time and intensity of the first-generated pulse are proportional to I$_{in}^{0.92}$ and I$_{in}^{0.81}$, respectively. A frequency shift by beam fanning is also discussed by observing interference pattern from an interferometer.

  • PDF

Conjugal Transfer of Plasmid DNA from Escherichia coli to Streptomyces lavendulae RFI-5

  • KITANI, SHIGERU;BIBB, MERVYN J.;NIHIRA, TAKUYA;YAMADA, YASUHIRO
    • Journal of Microbiology and Biotechnology
    • /
    • v.10 no.4
    • /
    • pp.535-538
    • /
    • 2000
  • Streptomyces lavendulae FRI-5 produces the ${\gamma}$-butyrolactone autoregulator IM-2, which is required for nucleoside antibiotic producetion. We have developed a system for introducing DNA into S. lavendule FRI-5 via conjugal transfer from Esherichia cole. Conditions were established for conjugation of the oriT-and attP-containing plasmid pSET152 from E. coli ET12567 (pUZ8002) to FRI-5. Conjugation resulted in integration of the plasmid at the chromosomal C31 attB site. The frequency of intergeneric conjugation varied with the medium used. The highest frequency ($1.6\times10-5$ per recipient) was obtained on ISP medium 2 containing 10mM MgCl2. Southern blot and phenotypic analyses of exconjugants revealed that S. lavendulae FRI-5 contains a unique C31 attB site, and that integration of heterologous DNA into the attB site did not interfere with morphological differentiation or IM-2-dependent signal transduction, including the production of a blue pigment. This system will now enable detailed genetic analysis of the regulation of antibiotic production in S. lavendulae FRI-5.

  • PDF

Factors affecting transfer frequency of pAM$\beta$$_1$from streptococcus faecalis to lactobacillus casei (Streptococcus faecalis의 pAM $\beta$$_1$을 lactobacillus casei로의 전이에 영향을 미치는 요인)

  • 허정원;강성훈
    • Korean Journal of Microbiology
    • /
    • v.28 no.3
    • /
    • pp.188-191
    • /
    • 1990
  • The streptococcal plasmid pAM$\beta_{1}$ (erythromycin resistance)was transferred via conjugation from Streptococcus faecalis DS 5 to Lactobacillus casei YIT 9018 by a filter mating method. The transfer frequency depended greatly on the type, pore size and side(front or back) of membrane filter. Water passing through a membrane under reduced pressure induced very tight contact between the cells, increased the transconjugation frequency about 1.3 to 37-fold when Millipore membrane filter (0.45.$\mu$m, front side up) was used.

  • PDF

Conjugal transfer and fate of the genetically engineered $Km^{r}$ gene in freshwater environments (유전자 조작기법으로 변형시킨 $Km^{r}$ 유전자의 담수 환경에서의 전이 및 행방)

  • 김치경;이성기
    • Korean Journal of Microbiology
    • /
    • v.28 no.3
    • /
    • pp.219-228
    • /
    • 1990
  • A kanamycin resistance($Km^r$) gene was studied for its transfer in natural freshwater environments by using the natural bacterial isolate(M1) of DK1 and the DKC601 strain, $Km^r$ plasmid of which was genetically engineered from the NI strain. The transfer frequency ofthe $Km^r$ gene and rearrangement of the $Km^r$ plasmid were compared between the gnetically engineered microorganism(GEM) and the NI parental strain by conjugation with the same recipient strain. The transfer frequency of the $Km^r$ gene was about $9.1\times 10^{-12}-1.8\times 10^{-11}$ in both the GEM and NI strains at 5 to $10^{\circ}C$, but the frequency of the NI was about 10 times higher than that of the GEM at 20 to $30^{\circ}C$. The $Km^r$ plasmid in the transconjugants obtained by conjugation of the NI with the MY1 strain as a ricipient showed alot of rearrangement, but the $Km^r$ plasmid transferred from the GEM was stable without alteration of its size. When the MT2 strain was used as a recipient, however, such a rearrangement of the $Km^r$ plamid was observed in the transconjugants obtained from the GEM as well as the NI strain. In those transconjugants obtained from different mating pairs and water environments, the plasmid were appeared to decrease in their number as the period of conjugation time was prolonged, but only the $Km^r$ plasmid transferred from the GEM kept having its size of 52kb. Therefore, the $Km^r$ gene was transferred at the same rate from the GEM and NI strains in natural freshwater environment, but the gene of the GEM strain was more stable than the NIduring conjugation and the $Km^r$ plasmid was rearranged by changing the recipient strain for conjugation in any water environments.

  • PDF

The R-Plasmid Transfer and Elimination of Shigella Cultures (Shigella균속의 항균제내성, 전달성 R-plasmid 및 제거에 관한 연구)

  • Hong, Sung-Ro;Lee, Yun-Tai
    • The Journal of the Korean Society for Microbiology
    • /
    • v.21 no.1
    • /
    • pp.33-45
    • /
    • 1986
  • On hundred and forty stains of shigella cultures isolated from the twelve hygiene laboratories of cities and provincial general hospital laboratories in 1983 were tested for their resistance to thirteen antimicrobial drugs and their R-plasmid transfer. Antimicrobial drugs were used amikacin, ampicillin, cephalothin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, rifampicin, streptamycin, tetracycline, tobramycin, cefoperazone and piperacillin. All strains were resistant to one or more of thirteen antimicrobial drugs but 94.3% were susceptible to amikacin, gentamicin and tobramycin of total isolated. The most strains commonly found resistance was to chloramphenicol (94%) followed by streptamycin (93%), tetracyline (92%) piperacillin (90%) ampicillin (83%), cefoperazone (42%), nalidixic acid (14%), cephalothin (17%), rifampicin (22%) and kanamycin (6%), sixty percent of strains among 140 were resistance to ampicillin, chloramphenicol, streptomycin, tetracycline at the same time. The transfer of drug resistance by conjugation was tested and ninety four strains (94.3%) were resistant to one or more drugs were found to transfer their drug resistance of E. coli. percentage of transfer frequency by conjugation was one strains (54%), the transfer frequency of drug resistance varied by donor strains and recipients, but not by selecting drugs. Resistance to nalidixic acid was not transferred by conjugation to recipients. Percentage of plasmid curing after the treatment of acriflavine, acridine orange was about 8%. Among strains cured two strains were tested compare original strains with them in biochemical properties in arginine dihydrolase and arabinose fermentation reaction. It was found to growth curves of No.2 shigella flexneri, serotype 1b, and its derivatives cured with acriflavine in $M{\ddot{u}}ller$ Hinton broth medium (pH 7.4, $38^{\circ}C$) by temperature Gradient Biophoto Recorder TN-1120 (Tokyo, Japan).

  • PDF

Transfer properties of nif-plasmid pEA 9 by pEA 9::Tn5-Mob (pEA 9::Tn5-Mob에 의한 nif-plasmid pEA 9의 transfer 성질)

  • 민병환;이호자
    • Korean Journal of Microbiology
    • /
    • v.26 no.3
    • /
    • pp.181-187
    • /
    • 1988
  • Using a Tn5-Mob system, pEA9 was characterized as a self-transmissible plasmid carrying a kanamycin resistance marker. The self-transfer frequencies of pEA9 varied greatly depending on pH values. The transfer frequency was about $4\times 10^{-5}$ at pH 5, that was 10 times higher than one at pH 6.5. With a helper plasmid, transfer frequencies were increased about $10^{4}$ times than the frequencies obtained without it.

  • PDF