• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2400-2402
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• 2014

Cu (II) detection is of great importance owing to its significant function in various biological processes. In this report, we developed a novel coumarin-based chemosensor bearing the salicylaldimine unit (2) for $Cu^{2+}$ selective detection. The results from fluorescence spectra demonstrated that the sensor could selectively recognize $Cu^{2+}$ over other metal cations and the detection limit is as low as $0.2{\mu}M$. Moreover, the confocal fluorescence imaging in HepG2 cells illustrated its potential for biological applications.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.159-163
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• 2004

This study was conducted to calculate the permeability coefficient in a single fracture while taking the true fracture geometry into consideration. The fracture geometry was measured using the confocal laser scanning microscope (CLSM). The CLSM geometry data were used to reconstruct a fracture model for numerical analysis using a homogenization analysis (HA) method. The HA is a new type of perturbation theory developed to characterize the behavior of a micro-inhomogeneous material that involves periodic microstructures. The HA permeability was calculated based on the local geometry and local material properties (water viscosity in this case). The results show that the permeability coefficients do not follow the theoretical relationship of the cubic law.

• ALGAE
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• 제25권1호
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• pp.11-15
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• 2010

Two new genera of red algae, Madagascaria erythrocladioides West et Zuccarello and Pseudoerythrocladia kornmannii West et Kikuchi (Erythropeltidales, Compsopogonophyceae, Rhodophyta), were previously described using molecular analysis and confocal microscopy of isolates in laboratory culture. We examined the ultrastructure of both genera to compare with ultrastructure of other members of the class Compsopogonophyceae. Both genera had Golgi bodies not associated with mitochondria and chloroplasts with a peripheral encircling thylakoid similar to all other members of the class studied thus far. Confocal autofluorescence images showed that Madagascaria has a single round central pyrenoid while Pseudoerythrocladia has no pyrenoid. Our electron microscopic work confirms these initial observations. Tables and keys are presented that assist in interpreting cellular details of genera in the class Compsopogonophyceae.

• 한국작물학회지
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• 제51권4호
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• pp.295-297
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• 2006

Potassium (K) distribution in rice (Oryza sativa L.) root was studied by confocal laser microscopy, using potassium sensitive fluorescent dye potassium-binding benzofuran isophthalate (PBFI). Significantly high intensity of K-specific fluorescence was detected at the root cap region followed by meristematic and basal regions. A negligible or fainted fluorescence was observed at the root hairs area. These results suggest that K is heavily distributed in the apical area of rice root, which may be required in higher concentration for division and extension of cells, as it is the rapidly growing region of the root, moreover, may also be involved in water uptake by creating osmotic gradient across membranes.

• Journal of the Optical Society of Korea
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• 제9권2호
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• pp.49-53
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• 2005

We report a new configuration of a reflection-type confocal scanning optical microscope system for measuring the refractive index profile of an optical waveguide. Several improvements on the earlier design are proposed; a light emitting diode (LED) at 650 nm wavelength instead of a laser diode (LD) or He-Ne laser is used as a light source for better index precision, and a simple longitudinal linear scanning and curve fitting techniques are adapted instead of a servo control for maintaining an optical confocal arrangement. We have obtained spatial resolution of 700 nm and an index precision of $2\times10^{-4}$. To verify the system's capability, the refractive index profiles of a conventional multimode fiber and a home-made four-mode fiber were examined with our proposed measurement method.

• Journal of the Optical Society of Korea
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• 제9권1호
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• pp.22-25
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• 2005

The differential heterodyne interferometer (DHI) is suitable for precise measurement of step height and line width, since its differential configuration can significantly reduce disturbances from the environment [1,2]. Like most phase measuring interferometers, however, the DHI is limited, in that it can obtain only the phase from 0 to 2π, because of the sinusoidal nature of the optical interference involved. Thus, the measurable step height is limited to one quarter of the wavelength of the light source. This study describes a confocal differential heterodyne interferometer (CDHI) for measuring step heights of several micrometers, with a high resolution and line width with high repeatability. The CDHI has a simple structure and rapid measurement speed.

• 한국광학회지
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• 제17권2호
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• pp.186-190
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• 2006

공초점 불안정형 공진기의 광학 정렬을 정밀하게 수행할 수 있는 새로운 개념의 null 렌즈 광학 정렬 방법을 제안하였다. 제안된 정렬 방법은 3.5 m 공진기에 적용하여 민감도 분석을 통해 실용성 여부를 판단하였으며, 공진기의 길이 변화가 1.0 mm 발생하였을 때와 반사경이 광축에 대하여 1.0 mrad 기울었을 때에 대한 pupil map 분석 데이터를 제시하였다.

• 한국광학회지
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• 제10권3호
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• pp.221-225
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• 1999

레이저선속의 퍼짐각을 개선하기 위하여 공진기 내부에 선속분할기를 갖는 양영역 공초점 불안정공진기 구리증기레이저를 구성하였다. 기하광학적 배율 M=30인 공진기에서 0.15 mard의 최소 퍼짐각을 얻었고, M=60인 공진기를 사용하여 0.1 mard 의 값을 얻을 수 있었다. 퍼짐각 개선과 관련하여 상대적인 원거리 출력밀도가 최대 130배 증가된 레이저 출력을 얻을 수 있었으며, 자체잠김현상을 보이는 펄스 형태의 레이저 발진이 관측되었다.

• 한국멀티미디어학회논문지
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• 제24권11호
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• pp.1472-1480
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• 2021

Image stacking technique is one of the key techniques for complex surface reconstruction. The process includes sample collection, image processing, algorithm editing, surface reconstruction, and finally reaching reliable conclusions. Since this experiment is based on laser scanning confocal microscope to collect the original contour information of the sample, it is necessary to briefly introduce the relevant principle and operation method of laser scanning confocal microscope. After that, the original image is collected and processed, and the data is expanded by interpolation method. Meanwhile, several methods of surface reconstruction are listed. After comparing the advantages and disadvantages of each method, one-dimensional interpolation and volume rendering are finally used to reconstruct the 3D model. The experimental results show that the final 3d surface modeling is more consistent with the appearance information of the original samples. At the same time, the algorithm is simple and easy to understand, strong operability, and can meet the requirements of surface reconstruction of different types of samples.

• Current Optics and Photonics
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• 제5권6호
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• pp.660-671
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• 2021

We present a novel optical system for an augmented reality head-up display (AR HUD) with two virtual images at different conjugates by employing a confocal off-axis two-mirror and introducing the horopter circle. For a far virtual image with large asymmetrical aberrations, we initially obtain an off-axis two-mirror system corrected for these aberrations and compensated for the down angle by configuring its parameters to satisfy the confocal and Scheimpflug conditions, respectively. In addition, this system is designed to reduce the biocular parallax by matching Petzval surface into the longitudinal horopter circle in a near virtual image. This design approach enables us to easily balance the residual aberrations and biocular parallax when configuring the optical system with two different conjugates, which results in an AR HUD available for near and far virtual images together.