• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권6호
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• pp.544-558
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• 2006

Trismus is a common problem to most people experiencing at once in his or her life and to most dental practitioners experiencing frequently. It has a number of potential causes which are single factor or complex factors. Its treatment will depend on the cause. The purpose of this study was to discuss the causes of trismus condition and the various treatments available. This study was made by reviewing of collected data from 86 patients complained of trismus among patients who were diagnosed by TMD, tumor, infection including tetanus, soft tissue anomalies, bony fracture and ankylosis from Jan 2002 to Dec 2004 on department of oral and maxillofacial surgery at Pusan National University Hospital, South Korea. The clinical reviews regarding chief complaints, clinical characteristics, diagnostic examination, treatments and the results on the patients were given as follows. 1. The etiology of trismus commonly were derived from temporomandibular joint(TMJ) disorder, TMJ ankylosis, TMJ tumor, odontogenic maxillofacial infection, mandibular condylar fracture, tetanus. 2. The chief complaints of trismus patients were progressive mouth opening limitation, TMJ pain, malocclusion, facial asymmetry, retrognathic state. 3. Especially, for the differential diagnosis between the fibrous ankylosis and true bony ankylosis, computed tomogram (CT) was useful. Surgical gap arthroplasty on bony ankylosis patients was applied and the gain of mouth opening after operation was average 35.8 mm during 19 months. 4. The tetanus, rarely, also induced the trismus with the range of mouth opening less than 10 mm. The average serum level of tetanus anti-toxin was 0.02-0.04 IU/mL. The limitation of mouth opening was improved into average 38 mm on 4 weeks after injection of 10,000 units of tetanus immune globulin. 5. In the treatment of osteochondroma, TMD, odontogenic infection and fracture, and the others inducing trismus, to obtian the maximum result and decreased inadequate time and effort, it is important to finding the causes from the exact clinical examination and diagnosis.

• 대한한의학회지
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• 제30권4호
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• pp.13-27
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• 2009

Objectives: Trans-cinnamaldehyde (TCA) is the main component of Cinnamomi Ramulus and it has been reported that TCA inhibits inflammatory responses in various cell types. Inflammation-mediated neurological disorders induce the activation of macrophages such as microglia in brain, and these activated macrophages release various inflammation-related molecules, which can be neurotoxic if overproduced. In this study, we evaluated gene expression profiles using gene chip microarrays in lipopolysaccharide (LPS)-stimulated BV-2 cells to investigate the antiinflammatory effect of TCA on inflammatory responses in brain microglia. Methods: A negative control group was cultured in normal medium and a positive control group was stimulated with $1{\mu}g/ml$ in the absence of TCA. TCA group was pretreated with $10{\mu}g/ml$ before $1{\mu}g/ml$ LPS stimulation. The oligonucleotide microarray analysis was performed to obtain the expression profiles of 28,853 genes using gene chip mouse gene 1.0 ST array in this study. Results: In positive control group, 1522 probe sets were up-regulated in the condition of the cutoff value of 1.5-fold change and 341 genes with Unigene ID were retrieved. In TCA group, 590 probe sets were down-regulated from among 1522 probe sets and 33 genes with Unigene ID were retrieved, which included 6 inflammation-related genes. We found out that Id3 gene is associated with transforming growth factor-${\beta}$ (TGF-${\beta}$) signaling pathway and Klra8 gene is related to natural killer cell-mediated cytotoxicity pathway. Conclusions: The results mean that TCA inhibits inflammatory responses through down-regulating the expressions of inflammation-related genes in LPS-stimulated BV-2 cells.

• 한국치위생학회지
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• 제11권5호
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• pp.717-727
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• 2011

Objectives : This study was purposed to estimate the level of oral health quality of life and the relationship between health-related factors and oral health quality of life in patients with successful dental implants. Methods : This questionnaire survey was conducted during the period from December 2009 to March 2010 with 126 patients confirmed successful dental implants in Busan. The oral health related quality of life and oral health-related factors were estimated with OHIP-14 and 8 oral health characteristics, respectively. Data analysis was performed with descriptive analysis, t-test, ANOVA, multiple regression analysis using SAS(ver 9.1) program. Results : The score of oral health quality of life in patients with successful dental implants was $12.25{\pm}7.82$. The scores of subscale of the oral health quality of life were $1.24{\pm}1.35$ in social disability, $1.60{\pm}1.37$ in handicap, $1.60{\pm}1.44$ in psychological disability, $1.70{\pm}1.38$ in functional limitation, $1.81{\pm}1.35$ in physical disability, $2.14{\pm}1.46$ in physical pain, $2.17{\pm}1.46$ in psychological discomfort, respectively. The related factors of oral health quality of life in patients with successful dental implants were absence of other type prosthesis and experience of tooth brushing education. Conclusions : The oral health related quality of life in patients with successful dental implants was relatively good condition. Social supports and chances for high quality denture and tooth brushing educations are needed to improve oral health related quality of life in patients with dental implants.

• Journal of Plant Biotechnology
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• 제38권2호
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• pp.162-168
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• 2011

고염 스트레스는 식물의 성장과 수확량에 치명적인 영향을 야기한다. 그와 같은, 환경 스트레스에 의하여 식물은 다양한 유전자의 발현으로 저항성을 가지게 하는 기작이 발달되어 있다. 본 연구에서는 애기장대에서 다양한 환경 스트레스에 관여하는 유전자를 분리할 목적으로 GGM(Graphical Gaussian Model) program을 사용한 후, BLH8(BEL1-Like Homeodomain Gene 8) 유전자의 돌연변이 식물체를 구축하였다. atblh8-1 돌연변이체는 고농도의 $Na^+$과 $K^+$ 이온에 특이적으로 백화현상을 보이지만, 뿌리 성장에는 변화를 보이지 않았다. 그러므로, BLH8 단백질은 $Na^+$과 $K^+$과 같은 환경스트레스 저항성에 관여하는 중요한 인자임을 시사한다. 이와 같이, GGM program은 환경 스트레스에 관여하는 유전자를 분리하기 위한 유용한 도구일 것으로 사려된다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제20권4호
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• pp.90-103
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• 2015

The agricultural soil, meets soil environmental standards whereas agricultural product from the same soil does not meet permissible level of contaminants, is identified in the vicinity of the abandoned mine in Korea. This study estimated the stabilization efficiency of Cd and Pb using limestone through the flood pot test for this kind of agricultural paddy soil. We had the concentration of the monitored contaminants in soil solution for 4 months and analyzed fractionations in soil and concentrations in rice plant. In soil solution of plow layer, the reductive Mn had been detected constantly unlike Fe. The concentrations of Mn in limestone amended soil was relatively lower than that in control soil. This reveals that the reductive heavy metals which become soluble under flooded condition can be stabilized by alkali amendment. This also means that Cd and Pb associated with Mn oxides can be precipitated through soil stabilization. Pb concentrations in soil solution of amended conditions were lower than that of control whereas Cd was not detected among all conditions including control. In contaminants fractionation of soil analysis, the decreasing exchangeable fraction and the increasing carbonates fraction were identified in amended soil when compared to control soil at the end of test. These results represent the reduction of contaminants mobility induced by alkali amendment. The Cd and Pb contents of rice grain from amended soil also lower than that of control. These result seems to be influenced by reduction of contaminants mobility represented in the results of soil solution and soil fractionation. Therefore contaminants mobility (phytoavailability) rather than total concentration in soil can be important factor for contaminants transition from soil to agricultural products. Because reduction of heavy metal transition to plant depends on reduction of bioavailability such as soluble fraction in soil.

• 생명과학회지
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• 제19권11호
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• pp.1651-1657
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• 2009

본 연구는 흰쥐의 흉추 9번을 타박 손상시키고 1주간의 휴식 후 스트레스를 유발하지 않는 저강도 트레드밀운동을 7주동안 수행하여, 가자미근 위축지연 변화를 western blotting과 H&E 염색 그리고 면역형광염색법으로 관찰하였으며, BBB-검사를 이용하여 기능적 회복을 관찰하였다. 형태학적 변화의 결과 척수손상 후 트레드밀 운동에 의해 하지부위 가자미 근과 근섬유의 횡단면 크기가 증가하였으며, 근섬유 세포에서 IGF-I 단백질 발현이 증가하였다. 또한 생화학적인 결과 척수손상 후 트레드밀 운동에 의해 하지부위 골격근 IGF-I 단백질 발현이 증가하였으며, 행동학적 검사를 통해 트레드밀 운동그룹이 비 운동그룹보다 기능적 회복이 개선된 것을 확인하였다. 이러한 결과는 근 비대에 직접적으로 관여하는 IGF-I 단백질 발현량이 운동에 의해 증가하여 가자미근크기 증가와 같은 근 위축 지연을 확인하였으며, 이러한 변화를 통해 기능적 회복에 긍정적인 결과를 보여주었다. 따라서 운동은 척수손상 환자에게 효과적인 기능재활 치료방법이 될 수 있으며, 장애를 최소화하여 자발적인 일상생활을 도모할 수 있을 것이라 생각된다.

• Fisheries and Aquatic Sciences
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• 제13권1호
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• pp.63-70
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• 2010

Two feeding experiments were conducted to investigate the effects of dietary genetically modified (GM) soya and com on growth performance, feed utilization and body composition of juvenile olive flounder, Paralichthys olivaceus and rockfish, Sebastes schlegelii. For each fish species, four isonitrogenous (50% crude protein) and isocaloric (4.1 kcal/g) diets (designated as nGM soya, GM soya, nGM com and GM com) were formulated to contain 20% non-GM (nGM) and GM soya and com. Thirty olive flounder (initial body weight, 15.4${\pm}$0.4 g) and fifty rockfish (initial body weight, 3.1${\pm}$0.02 g) were distributed in each 400 L tank (200 L water) in a flow through system. Each experimental diet was fed to triplicate groups of fish to visual satiation, twice a day (9:00 hand 17:00 h) for 6 weeks. Growth performance was measured every three weeks. No effects of GM feedstuffs on survival were observed. Dietary inclusion of GM feedstuffs did not affect growth performance and feed utilization of fishes, except for rockfish fed GM com. Rockfish fed the GM com diet showed higher weight gain, daily feed intake and daily protein intake than did fish fed the nGM com diet, but no significant differences were observed in final body weight between the dietary treatments. Condition factor, hepatosomatic index, visceral somatic index and body composition were not altered by the inclusion of GM feedstuffs. These results indicate that dietary inclusion of GM soya and com could have no effects on growth performance and feed utilization of juvenile flounder and rockfish. Lower weight gain and feed intake in flounder and rockfish fed the diets containing 20% soya were likely due to anti-nutritional factors, rather than transgenic factors in the feedstuffs. Dietary inclusion of GM soya and com at the level tested did not alter the body composition of fishes. Further studies to investigate the effects of GM feedstuffs on health conditions and the development of fishes, as well as those of residue of transgenic fragments in ambient environments and in animals are necessary for safe use of the ingredients in aquaculture.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권4호
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• pp.360-373
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• 2006

The purpose of this research was to investigate whether pulsed electromagnetic field (PEMF) stimulation applied to the rabbit cranial defects grafted with ${\beta}$-tricalcium phosphate (${\beta}$-TCP) could affect the new bone formation. With 16 New Zealand white rabbits under the same condition, bilateral calvarial bone defects were formed around the sagittal suture line. The defect on the left side was grafted with ${\beta}$-TCP, while on the right side was grafted by harvested autogenous bone. PEMF was applied to 8 rabbits for 8 hours per day. The bony specimen were divided into 3 groups, the group 1 was autogenous bone grafted specimen, the group 2 was ${\beta}$-TCP grafted with PEMF, and the group 3 was ${\beta}$-TCP grafted without PEMF. We investigated the bone regeneration & growth factor expression at 2, 4, 6, and 8 weeks. As a result, BMP 2 was expressed in the group 1 from 2 weeks, the group 2 from 4 weeks, and the group 3 from 6 weeks. BMP 4 was expressed in the group 1 from 2 weeks, in the group 2 and the group 3 from 4 weeks. 4. There was no significant difference in expression pattern of BMP 7, PDGF, VEGF, and TGF-${\beta}$1 during grafted bone regeneration in group 1, 2, and 3. According to our results, PEMF stimulation could be effective on the new bome formation in animal study, and have a feasibility of clinical use.

• 한국식품과학회지
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• 제38권1호
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• pp.147-151
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• 2006

본 연구에서는 저열량식이요법의 부작용 증세중 체력저하를 개선할 수 있도록 동의보감의 처방중 체력 증진과 연관된 보음(補陰), 보혈(補血) 기능을 갖는 사물탕(四物楊)을 기반으로 하여 개발된 생약 조성물의 체력 증진 기능을 규명하고자 실시하였다. 저열량식이요법의 모델로서 섭취 식이량의 50%를 제한하는 제한식이를 2주간 실시한 결과 제한식이를 섭취한 군은 강제 수영 모델에서 총 수영 시간이 감소하고 근육내 ATP 함량의 감소, 혈액중 젖산 함량의 증가가 나타나 식이 제한에 의해서 체력 감소가 나타남을 확인할 수 있었다. 본 연구에서 사용된 산야초 추출물을 동일하게 식이제한을 한 실험동물에게 섭취시킨 결과 총수영시간의 증가와 근육내 ATP의 증가, 젖산 함량의 감소가 나타나 저열량식이에 의한 부작용인 체력 저하를 산야초 추출물이 완화시킬 수 있는 기능이 있음을 확인 할 수 있었다. 또한, 산야초 추출물의 투여는 체력과 높은 연관성이 있는 것으로 알려진 혈중 IGF-1의 농도를 증가시키는 것이 관찰되어 체력 향상에 도움을 주는 것으로 추측된다. 식이 제한 환경에서 나타날 수 있는 천연물 추출물의 독성을 확인하기 위하여 간기능 지표인 GOT, GPT를 측정한 결과 특별한 변화가 나타나지 않아 산야초 추줄물의 안전성을 확인할 수 있었다.

• 대한예방한의학회지
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• 제5권2호
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• pp.40-68
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• 2001

In this study, grade of subjective symptom appealed by laborer of Jeollabuk-do was evaluated using questionary regarding factor made effect on musculoskeletal disease and in addition, studied relationship with health promotion life style of them. Based on the result, relationship of general characteristics of musculoskeletal subjective symptom and life-style of the subjects was concluded as below. 1. General characteristics of study subjects were as following. Ratio of male was higher as 57.7% of male and 42.2% female and age distribution was 5.1% of 20s, 34.99% of 30s, 36.3% of 40s and 23.7% of 50s and therefore, $30{\sim}40$ aged groups showed highest ratio. Most subjects (74.9%) was married status and in case of education level, high-school graduate and dropout (23.3%) and over-college graduate (46.8%) showed highest distribution. $1{\sim}2$ Mil. KRW (29.5%) and $2{\sim}2.99$ Mil. KRW (21.2%) is the main income distribution and however there was high ratio of non-reply (29.0%). In case of employment period, $10{\sim}14$ years (15.3%) and over 15 years (29.6%) showed highest ratio and there were many non-reply (39.4%) and in addition, 67.6% replied as own house and 14.3% as lease on deposit base in question of residence type. 2. Subjects showed high ratio of subjective symptom appeal of 62.79% and many cases (50.23%) appealed 1 or 2 symptoms. Symptom by body region was 29.8% (waist), 27% (shoulder), 21.2% (knee), 15.5% (neck), 9.5% (ankle), 8.1% (wrist) and 5.0% (elbow) in order. In case of relationship with general characteristics, female comparing with male, non-residence of own house, subjects with lower education level and employment period of $10{\sim}14$ years showed higher appeal rate and kind of symptoms than others. Therefore, it was concluded that rate of musculoskeletal symptom appeal have close relationship with gender, level of living, education level, age and employment period. 3. In case of severe pain of upper body except waist and ankle, it was appealed in both or right side and it means that upper body pain is originated from right side and right region pain is transited to both region pain. In addition, there was 39.41% of non-reply to existence of right-left region pain and therefore, it was evaluated that, in may cases, there was no awareness of their own symptom condition even on subjective symptom. 4. Degree of pain was, as pain over middle level, evaluated as 2.79 on full mark of 4.0 and in order of waist (2.97), ankle (2.83), knee (2.82), wrist (2.82), neck (2.79), shoulder (2.70) and elbow (2.62). In addition, 71.97% appealed $2{\sim}3$ cases for the latest 1 week. Owing to subjective symptom, 54.95% drop into hospital or pharmacy, 10.32% made temporary retirement or absence, 7.99% transferred into more comfortable duty and $39.4{\sim}54%$ experienced one or more managing mentioned above. 5. Fulfillment of health promotion life style of subjects was evaluated on full mark of 4.0 and total score was 2.63. Average mark of each area was personal relationship (3.05), self-realization (2.92), stress management (2.63), health control (2.48), physical exercise (2.19) and nutrition management (2.19) and personal relationship was highest and physical exercise and nutrition management were lowest. As general characteristics influencing health promotion life style, gender, residence style and employment period showed significant difference. Male showed higher mark than female and showed higher mark in order of own house, others, lease on deposit base, monthly rent. Subjects with longer employment period showed higher mark with significant difference. 6. Accounting of factor influencing each area of health promotion life style, self-realization showed significance in marriage status, income, residence style and education level and health control in age, residence style and employment period. Physical exercise showed significant difference in gender, age, residence style and employment period and nutrition in gender, age, residence style and employment period. Stress management showed significant difference in residence style and employment period and however not in personal relationship. 7. Health promotion life style relating with existence and kind of pain showed significant difference in all area except personal relationship area. In absence of pain, there was statistically significant high score in all area even in total health promotion life style and all area. Accounting of kind of pain, cases of $1{\sim}2$ kinds of pain and $5{\sim}6$ kinds of pain showed relatively high score and it was lower than mark of subject stated absence of pain. 8. Subjects appeal symptom were classified by symptom region and difference of total and each areas were evaluated. General area (p=0.002), self-realization (p=0.012), health management (p=0.023), physical exercise (p=0.028), nutrition management (p=0.028) and stress control (p=0.001) showed statistically significant difference and not in personal relationship area. Especially, elbow, shoulder and neck area marked high and group appealed pain of knee, arm and elbow, foot and ankle marked low. Based on those results, subjective symptom should be accounted seriously in diagnosis of occupational musculoskeletal disease of laborer and among subjective symptom, general characteristics of gender, age, condition of living, education level and employment period make effect. Generally subject appeal symptom marked lower than subject without symptom appeal and it means that life management of subject appealing musculoskeletal pain make important role in management and treatment of occupational musculoskeletal disease.