• Biomolecules & Therapeutics
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• v.1 no.2
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• pp.188-195
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• 1993

In order to understand the machanism of action and regulation of ${\beta}$-adrenergic receptor in terms of molecular level, the purification of receptor protein has a fundamental importance. Moreover, species differences among avian, amphibian and mammalian ${\beta}$-adrenergic receptors make it more important to purify mammalian ${\beta}$-adrenergic receptor. Because ${\beta}$-adrenergic receptor is an integral membrane protein, it must be solubilized from the membrane for the purification. The purpose of the present study was to solubilize and characterize the mammalian $\beta$-adrenergic receptor from guinea pig lung in quantities by more efficient and practical method eventually to purify receptor. Guinea pig lung membrane preparation was solubilized by sequential treatment of buffers containing low and high concentration of digitonin which are 0.2 and 1.2% respectively. About 50% of the total receptor pool was released by this double extraction procedure. The $\beta$-adrenoceptors in the digitonin extract were identified using the ${\beta}$-adrenergic antagonist, (-)-[$^3H$]-dihydroalprenolol ([$^3H$]DHA). The solubilized receptor retained all of the essential characteristics of membrane-bound receptor, namely saturability; stereoselectivity; high affinity to ${\beta}$-adrenergic drugs. For the measurement of soluble receptor activity, Sephadex G-50 chromatography method has been widely used. Inspite of its accuracy and wide acceptance, this technique employed troublesome column work which required long time to assay the activity of receptor. We employed another methods to measure receptor activity. When using 0.5% polyethylenimine pretreated GF/B glass fiber filter, filtration technique could be used to measure soluble receptor activity. This technique enabled us to reduce the total amount of time to assay by a factor of 4 as well as to detect soluble receptor. In the present study, we could establish more efficient and practical solubilization method of mammalian $\beta$-adrenergic receptor. The rapidity and high yield of this solubilization scheme, together with the favorable recovery of the receptor activity, are significant steps toward the ultimate purification of the mammalian $\beta$-adrenergic receptor. The result of this study together with more convenient purification method could provide large amount of purified receptor with ease for various research purposes.

• The Korean Journal of Cytopathology
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• v.7 no.1
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• pp.1-11
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• 1996

From the concepts of cellular pathology and of exfoliative cytology, as elucidated by Virchow and Papanicolaou respectively in the late 19th and early 20th century, have evolved the primary methods for the diagnosis of cancer today. From Papanicolaou's concept of exfoliative cytology developed fine needle aspiration biopsy in the early 1960's, this has become a major diagnostic procedure and has contributed to a significant reduction in open biopsies and, therefore, to medical cost-effectiveness immunobiochemical techniques provided us with a supplement to cancer diagnosis in the 1980's. The immunoperoxidase method, using monoclonal antibodies, is applied primarily as an ancillary measure to elucidate the nature of cancers The availability of specific monoclonal antibodies has greatly facilitated the identification of cell products or surface markers. For example, antibodies directed against intermediate filaments have proved to be of value in determining the histogenesis oi poorly differentiated neoplasms. Tumor markers may serve as biochemical indicators of the presence of a neoplasm. They can be detected In plasma and other body fluids. Their concentration can be applied as a diagnostic test, for monitoring the clinical course of known cancer, and as a screening measure to detect certain cancers in a population at risk. Flow cytometry is a useful tool for distinguishing several cell characteristics, such as the immunophenotype of leukemia-lymphoma cells, the DNA content of neoplastic cells, and cell proliferation rate. Molecular biologic techniques provided a giant step for the management of cancer patients encompassing diagnosis, prognostic evaluation, and therapy. Nucleic acid hybridization techniques are utilized as Southern, Northern, and dot blots and in situ hybridization. Molecular biology and its techniques may bring a blight new horizon for understanding cancer biology and in designing therapy on the basis of gene manipulation.

• Journal of Korean Tunnelling and Underground Space Association
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• v.8 no.3
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• pp.259-271
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• 2006

This paper aims to predict the corrosion of a fully cement-grouted rockbolt induced by chloride diffusion in a cement mortar grout. From the viewpoint of the long-term durability, a rockbolt may be deteriorated by chemical components, such as sulphate and chloride, in groundwater. Especially, the steel rod of a rockbolt is corroded mainly by chloride. The rockbolt corrosion results in the volume expansion of a rod and then the cracking of a cement grout. In this study, the chloride diffusion coefficient of a cement mortar grout was used to evaluate the possibility of rockbolt corrosion by chloride, and to predict the long-term durability of a rockbolt. The electric acceleration test method was adopted to measure the chloride diffusion coefficient. In addition, a simple pullout testing system was newly proposed to measure the pullout capacity of a rockbolt more easily in a laboratory condition. From the experiments, it was showed that the chloride could diffuse in the cement grout more easily than in ordinary concrete materials. As a result, it was considered that a rockbolt might be easily corroded in a short term by the diffusion of chemical components with high concentration, although it was fully grouted.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.1016-1025
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• 2009

Two experiments were conducted to determine energy and nutrient digestibility in four sources of distillers dried grains with solubles (DDGS) produced from corn and fed to growing pigs. The objective was to test the hypothesis that energy and nutrient digestibility in DDGS does not vary if samples are procured from ethanol plants that use similar production technologies and use corn that is grown within a narrow geographical area. The four sources of DDGS that were used were procured from ethanol plants that were less than 10 yr old and located within 250 km of each other. In Exp. 1, five growing barrows (initial BW = 71.4 kg) that were fitted with a T-canula in the distal ileum were allotted to a 5${\times}$5 Latin square design and used to measure apparent (AID) and standardized (SID) ileal digestibility of AA in the four sources of DDGS. Results of this experiment showed that the SID of CP and all AA except Cys and Pro were greater (p<0.05) in two of the DDGS sources than in the other two sources. Exp. 2 was conducted to measure the concentration of DE and ME and the apparent total tract digestibility (ATTD) of energy, N, P, ether extract, NDF, and ADF in corn and in the same four sources of DDGS as used in Exp. 1. Five pigs (initial BW = 29.7 kg) that were placed in metabolism cages and allotted to a 5${\times}$5 Latin square design were used. Results of Exp. 2 showed that the average DE and ME in DDGS were 4,072 and 3,750 kcal/kg DM, respectively, which was less (p<0.01) than the DE and ME in corn (4,181 and 4,103 kcal/kg DM, respectively). The average ATTD for P in DDGS was 56.1%, which was greater (p<0.01) than the ATTD for P in corn (31.9%). The ATTD for ADF in DDGS was also greater (p<0.05) than in corn, but the ATTD for ether extract and NDF were greater (p<0.05) in corn than in DDGS. It is concluded that energy and nutrient digestibility vary among sources of DDGS even when the DDGS is procured from ethanol plants that use corn grown within a narrow geographical region. Thus, factors other than corn growing region are responsible for the variability of energy and nutrient digestibility in DDGS.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.335-343
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• 2005

The Methodology of burnup calculation with EPMA test set up in this study. The spent fuel from PWR nuclear power plant was used as specimen. This $UO_2$ fuel with $3.2\%$ of enrichment had been irradiated up to 35,000 MWd/MTU(reference data). The burnup is very important factor for nuclear fuel to estimate all fuel behaviors in reactor. To measure amounts of fission products and actinides for the burnup calcualation, chemical analysis (destructive method) has been used but it mattes long experimental time and second radio-wastes. In this study, EPMA test was available to measure amount of fission products. Neodymium is able to be detected and quantified. It can be compared with the results from chemical analysis and ORIGEN-2 code calculation. Concentration of Nd from EPMA test showed good agreement with result of ORIGEN-2 code in the same burnup.

• Applied Microscopy
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• v.35 no.3
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• pp.113-119
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• 2005

The Methodology of burnup calculation with EPMA test set up in this study. The spent fuel from PWR nuclear power plant was used as specimen. This $UO_2$ fuel with 3.2% of enrichment had been irradiated up to 35,000 MWd/MTU. The burnup is very important factor for nuclear fuel to estimate all fuel behaviors in reactor. To measure amounts of fission products and actinides for the burnup calcualation, destructive method analysis has been used but it makes long experimental time and second radio-wastes. In this study, EPMA test was available to measure amount of fission products. Neodymium is able to be detected and quantified. It can be compared with the results from chemical analysis and ORIGEN-2 code calculation. Concentration of Nd from EPMA test showed good agreement with result of ORIGEN-2 code in the same burnup.

• Korean Journal of Optics and Photonics
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• v.28 no.4
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• pp.166-171
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• 2017

Hydrogen gas is a green energy sources because it features no emission of pollutants during combustion. But hydrogen gas is very dangerous, being flammable and very explosive. Hydrogen gas detection is very important for the safety of a nuclear power plant. Hydrogen gas is generated by oxidation of nuclear fuel cladding during a critical accident, and leads to serious secondary damage in the containment building. This paper discusses the development of a Raman lidar system for remote detection and measurement of hydrogen gas. A small, portable Raman lidar system was designed, and a measurement algorithm was developed to quantitatively measure hydrogen gas concentration. To verify the capability of measuring hydrogen gas with the developed Raman lidar system, experiments were carried out under daytime outdoor conditions by using a gas chamber that can adjust the hydrogen gas density. As results, our Raman lidar system is able to measure a minimum density of 0.67 vol. % hydrogen gas at a distance of 20 m.

• Korean Chemical Engineering Research
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• v.53 no.4
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• pp.496-502
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• 2015

Hydrocarbon dew point(HCDP), a property which is the most generally used for describing natural gas condensation, is a very important parameter of natural gas quality specifications. HCDP is strongly influenced by the concentration of the heavier hydrocarbon components, especially $C_6+$, so, along with compliance with gas quality specifications, build up of procedures for obtaining accurate HCDP is essential for gas transmission company, because hydrocarbon condensation present may cause serious operational and safety problems. This study has been carried out in an attempt to measure HCDP accurately by the automatic hydrocarbon dew point meter under the actual field conditions. Measured HCDP also has been compared with calculated HCDP using the composition determined by gas chromatograph and industry accepted equation of state at multiple pressures, along with the cricondentherm. The test results are 1) the automatic hydrocarbon dew point meter was able to measure stable HCDP continuously 2) the automatic hydrocarbon dew point meter has been performed reference measurement by pure propane with a known dew point from literature, and 3) A meaningful differences was observed when comparing the automatic hydrocarbon dew point meter and gas chromatograph results for synthetic standard gas mixtures and real gas mixtures.

• Proceedings of KOSOMES biannual meeting
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• 2007.05a
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• pp.21-26
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• 2007

The Protocol adopted in Sep. 1997 included the new Annex VI of MARPOL 73/78, which entered into force on 19 May 2005. Revision for the accept MARPOL Annex VI with make public on 29 Dec. 2005 and enforce on 29 Jun. 2006. For all that not installation at oil terminal or if installed do not used. The purpose of this study is to research and evaluate the methods of sampling and analysis, and the pollution measure of existed VOCs during the bulk liquid cargo operation at vessels. As the sampling instrument of VOCs, Tedlar bag has the effect of decrease in concentration by the chemical reaction and absorption, and the method using the solvent extraction has error cause because of the extraction, the absorption tube has error by the breakthrough. But canister is the best sampling instrument because of having no sampling mistake. The study on VOCs emission is not sufficient for the research on the pollution cause and the analysis data on the HAP during the bulk liquid cargo operation at vessels. Hence, monitoring on the management hazardous VOCs need to be continued during the cargo operation at vessels and VOCs management field must be study for the make progress at a good pace for reason of crew's health.

• The Journal of Engineering Geology
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• v.13 no.4
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• pp.405-418
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• 2003

Measurements of volumetric water content and saturation of porous media are very important factors in understanding the physical characteristics of soil, groundwater recharge by rainfall, pollutant movement, and slope failure. To measure such physical parameters, a permittivity method using electromagnetic wave is applied and use is made of the special permittivity response of understand to water and ethanol. In particular, the estimation is required because permittivity is influenced by the nature of the underground environment. In this study, we carried out experiments on the relative dependency of soil density, temperature and salinity of standard sand and granitic weathered soil using FDR-V system (Frequency domain reflectometry with vector network analyzer) within a frequency range of 1 - 18 GHz. The results of the study showed that the dielectric constants of standard sand and granitic weathered soil increased with increased volumetric water content of soil. However, the dependency of soil density was found to be a little low. Changes of dielectric constant with temperature appeared definitely in the real part of 1 GHz. That is, the dielectric constant of real part at 1 GHz of water and standard sand increased with the rise of temperature. However, ethanol showed decreased tendency. The study also showed that dielectric constant increased with increase in salinity at imaginary part of 1 GHz. It could be concluded from this study FDR-V system can adequately measure the physical properties of soil and the degree of salinity concentration of porous media within 1 GHz frequency range using dielectric constant.