• Title/Summary/Keyword: concentration measure

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Thermophoretic deposition of soot particles in laminar diffusion flame along a solid wall in microgravity (미소중력환경에서의 고체벽면근방 층류확산염내 매연입자의 열영동 부착)

  • Choi, Jae-Hyuk;Osamu, Fujita;Chung, Suk-Ho
    • 한국연소학회:학술대회논문집
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    • 2007.05a
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    • pp.19-24
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    • 2007
  • The deposition behavior of soot particles in a diffusion flame along a solid wall was examined experimentally by getting rid of the effect of natural convection utilizing microgravity environment. The microgravity environment was realized by using a drop tower facility. The fuel for the flame was an ethylene ($C_2H_4$) and the surrounding oxygen concentration 35% with the surrounding air velocity of $V_a$=2.5, 5, and 10 cm/s. Laser extinction method was adopted to measure the soot volume fraction distribution between the flame and burner wall. The results show that observation of soot deposition in normal flame was difficult from buoyancy and the relative position of flame and solid surface changes with time. The soot particle distribution region moves closer to the surface of the wall as the surrounding air velocity is increased. And the experiments determined the trace of the maximum soot concentration line. It was found that the distance between soot line and flame line is around 5 mm. That is, the soot particle near the flame zone tends to move away from flame zone because of thermophoretic force and to concentrate at a certain narrow area inside of the flame, finally, to adhere the solid wall.

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Hydrogen Peroxide, Its Measurement and Effect During Enzymatic Decoloring of Congo Red

  • Woo, Sung-Whan;Cho, Jeung-Suk;Hur, Byung-Ki;Shin, Dong-Hoon;Ryu, Keun-Gap;Kim, Eun-Ki
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.773-777
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    • 2003
  • The color of Congo red hinders the spectrometric measurements of a concentration of hydrogen peroxide and enzyme activity (Horseradish peroxidase; HRP) during enzymatic decoloring of Congo red. In this study, a method was developed to measure peroxidase activity and hydrogen peroxide concentration in the presence of Congo red. The oxidation product of HRP/hydrogen peroxide and ABTS(2,2'-azino-bis-(3-ethylbenzotriazoline-6-sulfonic acid)) formed a dark green color. The spectrum of this product showed absorption bands at 420 nm and 734 nm. When compared with the Congo red spectrum, the absorption at 734 nm of this product did not overlap with Congo red, thus making the hydrogen peroxide measurement possible even in the presence of Congo red. Kinetic study of decoloring of Congo red performed by this method showed that the decoloring reaction followed the Michaelis-Menten kinetics. Pulse feeding of hydrogen peroxide, upon depletion, significantly increased the decoloring of Congo red. This result shows that this newly developed technique can monitor, predict, and improve the enzymatic decoloring process.

Estimation of Sea Surface Current Vector based on Satellite Ocean Color Image around the Korean Marginal Sea

  • Kim, Eung;Ro, Young-Jae;Ahn, Yu-Hwan
    • Proceedings of the KSRS Conference
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    • v.2
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    • pp.816-819
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    • 2006
  • One of the most difficult parameters to measure in the sea is current speed and direction. Recently, efforts are being made to estimate the ocean current vectors by utilizing sequential satellite imageries. In this study, we attempted to estimated sea surface current vector (sscv) by using satellite ocean color imageries of SeaWifs around the Korean Peninsula. This ocean color image data has 1-day sampling interval and spatial resolution of 1x1 km. Maximum cross-correlation method is employed which is aimed to detect similar patterns between sequential images. The estimated current vectors are compared to the surface geostrophic current vectors obtained from altimeter of sea level height data. In utilizing the color imagery data, some limitations and drawbacks exist so that in warm water region where phytoplankton concentration is relatively lower than in cold water region, estimation of sscv is poor and unreliable. On the other hand, two current vector fields agree reasonably well in the Korean South Sea region where high concentration of chlorophyll-a and weak tide is observed. In the future, with ocean color images of shorter sampling interval by COMS satellite, the algorithm and methodology developed in the study would be useful in providing the information for the ocean current around Korean Peninsula.

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Vacuum Pressure Treatment of Water-Soluble Melamine Resin Impregnation for Improvement of Dimensional Stability on Softwoods (목재의 치수안정성 개선을 위한 진공가압 멜라민 수지함침처리)

  • Oh, Seung-Won;Park, Hee-Jun
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.3
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    • pp.327-333
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    • 2015
  • To measure the dimensional stability of three softwoods by vacuum pressurization of water soluble melamine-formaldehyde (MF) resin impregnation process, properties of resin treated compressed woods (compregs) were measured after impregnating the water soluble MF resin into three coniferous wood species for different impregnation times and resin concentrations. As the resin concentration was higher and impregnation time was longer, coefficients of volumetric shrinkage and anti-volumetric swelling efficiency increased, but coefficients of volumetric swelling, anti-volumetric swelling efficiency and absorption decreased. Also, weight percent gain increased remarkably as the resin concentration was high, but there was no uniform relationship with impregnation time.

Optimization of the Lowry Method of Protein Precipitation from the H. influenzae Type b Conjugate Vaccine Using Deoxycholic Acid and Hydrochloric Acid

  • Kim, Hyun-Sung;Kim, Sang-Joon;Kim, Hui-Jung;Kim, Han-Uk;Ahn, Sang-Joem;Hur, Byung-Ki
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.3
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    • pp.215-222
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    • 2006
  • The Lowry method was used in this study to measure protein in Haemophilus influenzae type b (Hib) conjugate vaccines (polyribosylibitol phosphate-tetanus toxoid; PRP-TT) using deoxycholic acid (DOC) to induce protein precipitation. Trichloroacetic acid (TCA) did not induce precipitation adequately from the Hib conjugate bulk and the freeze-dried Hib conjugate product. Its yield was approximately 50%. The matrix structure of Hib conjugate inhibits precipitation by TCA. Although the Lowry method can be carried out without precipitation in Hib conjugate bulk when no residual impurities (adipic acid dihydrazide [ADH], 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide-HCI [EDAC], phenol and cyanogens bromide [CNBr], etc.) are present, it cannot be used for Hib conjugate products that contain sucrose 8.5%, because 8.5% concentration of sucrose enhanced the protein concentration. DOC- and HCl-induced precipitation is an alternative method for evaluating the protein content of the Hib conjugate bulk and the Hib conjugate product. The precipitation was optimal with a final concentrate of 0.1% for DOC at $4^{\circ}C$ and pH 2. This Lowry method, using DOC/HCI precipitation to induce protein precipitation, was confirmed a consistent, reproducible, and valid test for proteins in Hib conjugate bulk and its freeze-dried product.

Ring type non-invasive glucose sensor (민감도가 향상된 링 형태의 비 침습식 혈당 센서)

  • Lee, Ki-Hyuck;Lee, Kook-Joo;Lee, Dong-Ho;Kim, Moon-Il;Park, Gil-Hong
    • Journal of IKEEE
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    • v.11 no.4
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    • pp.293-296
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    • 2007
  • A novel sensitive and non-invasive ring-type glucose probe was designed and measured. The magnitude and phase of return loss change with different concentration of the glucose solution inside the loop structure. Ring type probe is more advantageous than open ended probe to measure electrical characteristics under the skin. The maximum difference of return loss was 0.94 for the concentration difference of 0% and 20% at 285MHz and more sensitive in low frequency.

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Effect of Grape Skin Extract on Apoptosis in MDA-MB-231 Human Breast Cancer Cells (포도껍질 추출물이 인체유방암세포 사멸에 미치는 영향)

  • Park, Min-A;Kang, Keum-Jee
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.1
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    • pp.87-98
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    • 2015
  • We investigated the apoptotic effects of grape skin extracts (GSE) and related gene expressions in human breast cancer MDA-MB-231 cells cultured in the presence of 0, 0.5, 1 and 1.5 mg/mL of GSE for 72 hours. MTT assay, trypan blue and nuclei staining showed lower cellular mitochondrial activities and increased cell deaths with a higher concentration of GSE (p<0.05). Increased cell number with fragmentated DNA of sub-G1 phase was calculated as a measure of apoptotic cell death by FACS analysis (p<0.05). In particular, apoptotic cell death caused markedly increased in the 1 and 1.5 mg/mL of GSE groups, as revealed by flow cytometry (Annexin V-FITC). RT-PCR analysis was performed on apoptotic and preapoptotic genes. Expression of the apoptosis suppressor gene bcl-2 significantly decreased, proapoptotic gene bax was significantly increased and procaspase-3 showing the presence of caspase-3 significantly decreased (p<0.05). Furthermore, bcl-2/bax ratio which is considered to be an important indicator of apoptosis, significantly decreased in a concentration-dependent manner (p<0.05). These results indicated that GSE induces apoptosis in MDA-MB-231 human breast cancer cells.

Study of Antimicrobial Activity of New Zealand's Tea Tree Essential Oil, Grapefruit Seed Extract and its major Component.

  • Han, Chang-Giu;Lee, Young-Woon;Zhoh, Choon-Koo;Kim, Byung-Hoon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.25 no.4 s.34
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    • pp.17-41
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    • 1999
  • Manuka oil sometime named New Zealand's tea tree oil is soluble in oil and come from nature. The $\alpha$-pinene extracted from Manuka oil and R-limonene which is one of the component of extracted Citrex from Grapefruit were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion and broth dilution methods were used to measure the antimicrobial activity. Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus is similar when the concentration of Manuka oil and $\alpha$-pinene is $10{\mu}l$. However, Antimicrobial activity of Manuka oil for Escherichia coli, Staphylococcus aureus is better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene is low. Antimicrobial activity of Citrex is superior to that of R-limonene. The proper ratio of Maunka oil and Citrex can improve the antimicrobial activity. The proper ratio obtained from studies was 75% of Maunka oil and 25% Citrex for Escherichia coli, 25% of Maunka oil and 75% Citrex for Staphylococcus aureus.

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Performance Test and Flue Gas Characteristics of a 350 kW Wood Pellet Boiler (350 kW(300,000 kcal/h)급 우드 펠렛 보일러 운전 특성 및 성능 평가)

  • Kim, Jong-Jin;Kang, Sae-Byul
    • Proceedings of the SAREK Conference
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    • 2009.06a
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    • pp.167-171
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    • 2009
  • We conducted performance test of a 350 kW class wood pellet boiler installed at a dormitory whose total area is $1,354\;m^2$. The maximum heating capacity of the boiler is 350 kW(300,000 kcal/kg). The wood pellet boiler consists of 3 parts; boiler, hot water storage tank and wood pellet storage tank. In testing the boiler, we shut off hot water utility supply and open up floor heating water system in order to measure exact value of the heating output of the wood pellet boiler. To determine the efficiency and heating output of the wood pellet boiler, we measured mass flow rate of wood pellet, the lower heating value(LHV) of the wood pellet, mass flow rate and temperature of water for floor heating and so on. We measured the mass flow rate of fuel, wood pellet with respect to rotational speed of auger, wood pellet feeding screw. We also measured the flue gas concentration of the wood pellet boiler by using a gas analyser. The result shows that the efficiency of the wood pellet boiler is 80.6% based on lower heating value at 124 kW of heating output. At this condition, O2 concentration of the flue gas is 6.0%, CO and NOx concentrations are 85 and 102 ppm.

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Characterization of the Heavy Metals Contaminating the River Nile at El-Giza Governorate, Egypt and Their Relative Bioaccumulations in Tilapia nilotica

  • Morgan, Ashraf M.; Shin, Ho-Chul;Aty, A.M. Abd El
    • Toxicological Research
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    • v.24 no.4
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    • pp.297-305
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    • 2008
  • This study was carried out to measure the concentration of heavy metals (Pb, Mn, Cr, Cd, Ni, Zn, and Cu) in water and Bolti fish (Tilapia nilotica) samples collected from Rasheed branch of River Nile, north of El-Giza Governorate, Egypt by atomic absorption spectrophotometry. The investigated districts through which the branch passes include El-Manashi, Gezzaya, El Katta, Abo Ghaleb and Wardan. Based on WHO and FAO safety reference standards, the results of the current study showed that water and fish tissues were found to contain heavy metals at significantly variable concentration levels among the investigated districts. They were polluted with respect to all the metals tested at Gezzaya district. However, the levels of analyzed metals in water and fish tissues were found lower than legal limits in other districts. The heavy metals showed differential bioaccumulation in fish tissues of the different districts as the accumulation pattern (as total heavy metal residues) was district dependant as follow: Gezzaya > Wardan > El Katta > Abo Ghaleb > El Manashi.