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Preparation and Characterization of Salmon Patty using Muscle from Salmon Frame (연어 frame 유래 근육을 이용한 연어 패티의 제조 및 특성)

  • Heu, Min-Soo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.3
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    • pp.183-189
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    • 2009
  • This study was conducted to prepare salmon patty using muscle separated from salmon frame (SPFM) and to investigate the food component characterization. When compared to salmon patty with fillet muscle (SPM), SPFM was lower in the moisture content, while it was higher in crude lipid content. However, no differences in the ash and protein contents between SPFM and SPM were found. Compared to SPM, the Hunter color value in cross section of cooked SPFM was higher in a and $\Delta$E values, while the color was lower in Land b values. Trichloroacetic acid soluble-N content of SPFM was 279 mg/100 g, which was insignificantly different (P>0.05) compared to those of SPM and commercial patty. The hardness of SPFM was 0.44 kg/$cm^2$, which was insignificantly different (P>0.05) compared to that of SPM, while was higher than that of commercial patty. The major fatty acids of SPFM were 16:0 (16.5%), 18:1n-9 (29.2%) and 18:2n-6 (26.1%). The 20:5n-3 and 22:6n-3 were also detected in high composition. The total amino acid content of SPFM was 16.6 g/100 g, which was similar to that of SPM. However, the total amino acid of SPFM was 14% higher than that of commercial patty. From the results of the mineral content, SPFM was higher than that of SPM in Fe and Ca, while the K in SPFM was lower. According to the result of sensory evaluation on the color, flavor and taste, no significant differences in all sensory items between SPFM and SPM were found.

A single-step isolation of useful antioxidant compounds from Ishige okamurae by using centrifugal partition chromatography

  • Kim, Hyung-Ho;Kim, Hyun-Soo;Ko, Ju-Young;Kim, Chul-Young;Lee, Ji-Hyeok;Jeon, You-Jin
    • Fisheries and Aquatic Sciences
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    • v.19 no.4
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    • pp.22.1-22.7
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    • 2016
  • One of the main compounds in Ishige okamurae, diphlorethohydroxycarmalol (DPHC), is known to exhibit antiviral and anti-inflammatory effects. However, it has not been investigated extensively. In this study, preparative centrifugal partition chromatography (CPC) coupled with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) online HPLC was employed for effectively separating considerable amounts of antioxidant compounds from marine algae. Two main antioxidant compounds, DPHC and octaphlorethol A (OPA), respectively, were confirmed and isolated from the ethyl acetate (EtOAc) fraction of I. okamurae by $ABTS^+$ online HPLC and preparative CPC systems. The presence of DPHC and OPA was confirmed in the EtOAc fraction of I. okamurae by both liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI/MS) and $ABTS^+$ online HPLC systems: DPHC (39 mg) and OPA (23 mg) were successfully isolated from I. okamurae (500 mg) with optimum solvent composition (0.5:10:4:6; n-hexane/EtOAc/MeOH/water, v/v) with corresponding partition coefficients (K) of 1.62 and 2.71, respectively, by preparative CPC. Hence, CPC coupled with $ABTS^+$ online HPLC is convenient for the efficient and simple isolation of these antioxidant compounds from I. okamurae.

Production of Environment-friendly Artificial Media for Agriculture Using Urban Sludge (도시발생 슬러지를 이용한 환경친화적 인공배지 생산)

  • 김선주;윤춘경;양용석
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.40 no.2
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    • pp.102-111
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    • 1998
  • Large amount of sludge have been generating in the process of water and wastewater treatment in urban area, and it has been making many environmental problems. Currently almost of sludge is landfilled, and since sludge is difficult to handle and dehydrate, the permeated water from the filled-in ground contaminate the surrounding soil and groundwater which may cause serious environmental and sociological problems. The organic component in sludge can be almost removed through the heat treatment process, and the final product is called artificial soil or artificial media according to the temperature control. To produce artificial media using sludge, chabazite and lime were used as an additive, and the mixture of sludge & additives was thermally treated in the firing kiln at about 800~1, 100。C for about fifteen minutes. The physical and chemical characteristics of the produced artificial media were analyzed, and it showed that it can be used as an artificial media for plant production or soil conditioner for farmland. The concentrations of the toxic heavy metals in the artificial media were lower than the soil quality standard for farmland. The characteristics of produced artificial media, using the mixture of sludge and additives through the heat treatment, is similar to the natural chabazite and soil. The analyzed result of the mineral composition of artificial media showed that it has a characteristics similar to natural stable soil, so the produced artificial media may be applied to farmland or water culture without causing adverse effect. Therefore this study showed that the above process can be a feasible alternative for sludge treatment.

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Purification and Characterization of ${\beta}-Glucosidase$ from Penicillium verruculosum

  • Chun, Soon-Bai;Kim, Dong-Ho;Kim, Kang-Hwa;Chung, Ki-Chul
    • Journal of Microbiology and Biotechnology
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    • v.1 no.3
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    • pp.188-196
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    • 1991
  • The ${\beta}-glucosidase$ was purified to homogeneity from the culture filtrate of P. verruculosum by column chromatography. The enzyme was a glycoprotein with a relative size of approximately 220 kDa with an isoelectric point of 4.8, which was composed of dimeric protein of 105 kDa. The enzyme was stable up to $60^{\circ}C$ and the presence of glycerol significantly increased its thermostability. The enzyme was found to hydrolyze both ${\beta}-aryl$ and ${\beta}-alkyl-glucosides$ in addition to ${\beta}-glucosyl$ glucose and catalyzed glucosyl transfer to cellobiose. The enzyme attacked laminarin in an exotype-like fashion. The apparent Km's of the enzyme toward cellobiose, laminaribiose, laminarin were 0.53 mM, 0.35 mM and 1.11 mM, respectively. Glucose and glucono-${\delta}-lactone$ were competitive inhibitors for the enzyme. Copper ($Cu^{2+}$), mercury ($Hg^{2+}$) and p-chloromercuribenzoate were strong inhibitors of the enzyme. The immunoblotting result revealed that one form of ${\beta}-glucosidase$ was biosynthesized, irrespective of carbon sources used. Polyacrylamide gel electrophoresis analysis of the in vitro translated product of total RNA from avicel grown mycelium established that the P. verruculosum ${\beta}-glucosidase$ precursor was approximately 95 kDa in size. The amino acid composition and N-terminal amino acid sequence are given.

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Changes in Quality of Seasoned and Smoked Squid During Processing (조미훈연 오징어의 가공중 품질변화)

  • RYU Hong-Soo;MUN Sook-Im;LEE Kang-Hoo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.25 no.5
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    • pp.406-412
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    • 1992
  • Changes in proximate composition and protein quality were determined to find out appropriate processing conditions of the seasoned and smoked squid(Neon flying squid, Ommastrephes bartrannii). Moisture and crude protein contents were severely reduced (p<0.05), while increasing of fat and ash contents were not apparent. Seasoning and smoking contributed iii enhancing TBA value. Trypsin inhibitor(Tl) content was not increased severely after those processing steps. TI content checked in the all steps of squid processing was not correlated with the TBA value of squid in the same processing step. An improved digestibility and protein efficiency ratio(PER) were observed in the all products except with steak(mechanically soften product) in vitro enzymatic digestibilities of both raw Neon flying squid meats(mantle and arm) were significantly inferior(p<0.05) to other squid species.

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A Study on the Combination of Household Interior Space Application Using Carved Ceramic Candlestick with the Motive of Yeonga (연가를 모티브로 한 도자 투각 촛대와 이를 이용한 주거 실내공간 인테리어 활용 융합방안에 관한 연구)

  • Kim, Seung Man
    • The Journal of the Korea Contents Association
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    • v.18 no.5
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    • pp.472-481
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    • 2018
  • The main point of this paper is to examine the composition of interior space of the residence centered on carved ceramic candlestick with the motive of Yeonga. And with the Yeonga candlestick developed using that, the application of space convergence in the interior design is researched. As the industry develops, pleasant and comfortable living environment draws attention in choosing residence with the psychology to engage nature into the house working. It is anticipated that the Yeonga candlestick interior accessories will be used to smoothly connect floor, wall and ceiling. As the shape of Yeonga includes the aura of the luminaire, the design element of Yeonga is researched through it. These sources are applied to the carved candlestick to investigate their application as indoor lighting. It is also a major viewpoint to develop this as a ceramic product and actively utilize it as a necessary interior item in daily life.

Microstructural Development in Synthetic Hydroxyapatite (합성 수산화아파타이트의 미세구조 발달)

  • Kim, Jong-Hee;Park, Young-Min;Yang, Tae-Young;Yoon, Seog-Young;Park, Hong-Chae
    • Journal of the Korean Ceramic Society
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    • v.41 no.4
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    • pp.289-296
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    • 2004
  • Whisker and short rod shaped hydroxyapatite (Ca$_{10}$(PO$_4$)$_{6}$(OH)$_2$ with stoichiometric composition (Ca/P=1.62 -1.67, molar ratio) has been synthesized by hydrolysis and hydrothermal reaction of aqueous $\alpha$-Ca$_3$(PO$_4$)$_2$($\alpha$-TCP) solution (pH 11), respectively. The shape of resultant HAp was mainly dependent on synthetic route and the microstructural development was on processing condition. In hydrolysis processing, the degree of intersection of whiskerlike particles and agglomeration in the apsis line increased with increasing reaction time. In hydrothermal synthesis, the reaction product obtained under excessive reaction time ($\geq$3 h at 20$0^{\circ}C$) was severely agglomerated without further grain growth above certain critical size (0.75 ${\mu}{\textrm}{m}$ in length, 0.3${\mu}{\textrm}{m}$ 11m in diameter).

Multiculturalism and Socio-Spatial Segregation of Honolulu in the 1920s (1920년대 호놀룰루의 다문화주의와 집단간 사회-공간적 분리)

  • Lee, Young-Min
    • Journal of the Korean Geographical Society
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    • v.42 no.5
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    • pp.675-690
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    • 2007
  • It has been widely believed that the ethnic relations in Honolulu and Hawai'i in the early twentieth century were little associated with racist ideology because the white race was minority in terms of the racial composition. In reality, however, the racial and ethnic issues have played a major role in forming the past and present relations among ethnic groups. This study shows that the white-supremacy ideology exerted a strong influence on minority groups in Honolulu throughout the immigration and settling-down process, as much as in the mainland U.S. Clear occupational stratification and residential segregation among the ethnic groups in Honolulu represented almost the same situation as in mainland cities. The social segregation and spatial propinquity of their residential neighborhoods facilitated the construction of dichotomized identity: "Local" versus "Haole". Such transformed identities were a product of on-going inter-ethnic negotiation process embedded in the non-white multi-ethnic neighborhoods.

Investigation of Al-Ni Alloys Deposition during Over-discharge Reaction of Na-NiCl2 Battery

  • Kim, Jeongsoo;Jo, Seung Hwan;Park, Dae-In;Bhavaraju, Sai;Kang, Sang Ook
    • Journal of the Korean Electrochemical Society
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    • v.19 no.3
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    • pp.57-62
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    • 2016
  • The over-discharging phenomena in sodium-nickel chloride batteries were investigated in relation to decomposition of molten salt electrolyte and consequent metal co-deposition. From XRD analysis, the material deposited on graphite cathode current collector was revealed to be by-product of molten salt electrolyte decomposition. In particular, the result showed that the Ni-Al alloys ($Al_3Ni_2$, $Ni_3Al$ and $Al_3Ni$) were electrochemically deposited on graphite current collectors in line with over-discharging behaviors. It is assumed that the $NiCl_2$ solubility in molten salt electrolytes leads to the co-deposition of Ni-Al alloys by increasing metal deposition potential above 1.6 V (vs. $Na/Na^+$). The cell tests have revealed that the composition of molten salt electrolytes modified by various additives makes a decisive influence on the over-discharging behaviors of the cells. It was revealed that NaOCN addition to molten salt electrolytes was advantageous to suppress over-discharge reactions by modifying the characteristics of molten salt electrolytes. NaOCN addition into molten salt electrolytes seems to suppress Ni solubility by maintaining basic melts. The cell using modified molten salt electrolyte with NaOCN (Cell D) showed relatively less cell degradation compared with other cells for long cycles.

The Effects of Exercise-based Cardiac Rehabilitation Phase II on Pro- and Anti-inflammatory Markers in Patients with Acute Coronary Syndrome

  • Kim, Al-Chan;Oh, Jae-Keun;Shin, Kyung-Ah;Kim, Young-Joo
    • Biomedical Science Letters
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    • v.19 no.1
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    • pp.61-69
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    • 2013
  • The aim of this study was to evaluate the effect of exercise-based cardiac rehabilitation on pro- and anti-inflammatory markers in patients with acute coronary syndrome (ACS). ACS patients who underwent percutaneous coronary intervention (PCI) and took medicine during phase II of rehabilitation were recruited for study. Subjects were divided into two groups; exercise group (EX, n=21) and a non-exercise group (non-EX, n=13). Supervised exercise program in hospital consisted of treadmill and bicycle exercise was performed three times per week for 6 weeks. Patients of EX received individual counseling, including knowledge of heart disease, risk factor modification, and physical training. Cardiopulmonary fitness, body composition, and biochemical blood factors were analyzed before and after experiment. There was no significant difference in serum levels of hs-CRP and TGF-${\beta}1$ between groups, and between time intervals. But there was a significant decrease in serum levels of IL-18 (P<.001). And there was a significant increase in ratio of IL-18 to IL-10 (P<.01) and serum levels of IL-10 (P<.001). After cardiac rehabilitation, there was significant increase in exercise duration (P<.001), maximal oxygen uptake ($VO_{2peak}$; P<.001) and decrease in submaximal rate-pressure product (sRPP; P<.05) in EX. In conclusion, exercise-based cardiac rehabilitation during phase II in patients with ACS after PCI decreased serum IL-18 (pro-inflammatory) content and ratio of IL-18 to IL-10 in serum (highly related with disease recurrence), and increased serum IL-10 (anti-inflammatory) content. In addition, it led to improved cardiopulmonary fitness.