• Title/Summary/Keyword: composition gradient

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An Overview of Different Techniques on the Microbial Community Structure, and Functional Diversity of Plant Growth Promoting Bacteria

  • Kim, Kiyoon;Islam, Rashedul;Benson, Abitha;Joe, Manoharan Melvin;Denver, Walitang;Chanratan, Mak;Chatterjee, Poulami;Kang, Yeongyeong;Sa, Tongmin
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.2
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    • pp.144-156
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    • 2016
  • Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

Plant Assemblages Along an Altitudinal Gradient in Northwest Himalaya

  • Gupta, Bhupendar;Sharma, Navneet
    • Journal of Forest and Environmental Science
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    • v.31 no.2
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    • pp.91-108
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    • 2015
  • The study investigates herbage communities along the altitudinal gradient in northwest Himalaya and highlights the effect of trees on its understory floristic diversity and phytosociology. The study was conducted in nine forests at three elevations viz., chir pine (Pinus roxburghii), mixed and khair (Acacia catechu) forests at elevation $E_1$ (850-1150 m), chir pine, mixed and ban oak (Quercus leucotrichophora) forests at elevation $E_2$ (1151-1600 m) and ban oak, mixed and chir pine forests at elevation $E_3$ (>1600 m) in a sub-watershed located in Solan district of Himachal Pradesh, India. These were compared grasslands located adjacent to forests. In all, 20 grass, 3 sedge, 2 forb and 4 legume species were recorded in study sites. Jaccard's coefficient of herbage vegetation was highest between chir pine forests and grasslands, and lowest in khair and ban oak forests. TWINSPAN dendrogram of herbage composition exhibited three subtypes with Apluda mutica, Arundinella nepalensis and Dichanthium annulatum as indicator species. Three groups of plant communities were identified on the basis of their moisture requirement. Peak density and basal area of herbage in forests and grasslands occurred by September. Density and basal area of herbage in grasslands at different elevations ranged from 649.6 to $1347.9tillers/m^2$ and 30.0 to $65.7cm^2/m^2$, respectively, while, in forests it varied from 351.2 to $1005.3tillers/m^2$ and 14.9 to $43.9cm^2/m^2$, respectively. Density and basal area of the herbage in plant communities decreased along the elevation. Under trees in forests the density of herbage decreased up to 77% and basal area up to 62% of their respective values in grasslands.

Solidification and Segregation Behaviors with Solidification Rate in Co base superalloy, FSX-414 (일방향 응고된 Co기 초내열합금 FSX-414의 응고속도에 따른 응고조직 및 편석 거동)

  • Lee, Hyun-Jung;Lee, Je-Hyun;Seo, Seong-Moon;Jo, Chang-Young;Gwon, Seok-Hwan;Chang, Byeong-Moon
    • Korean Journal of Metals and Materials
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    • v.47 no.7
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    • pp.440-446
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    • 2009
  • Co base superalloys have been widely used for the parts of gas turbine due to their excellent strength, thermal fatigue, oxidation resistance and weldability at high temperature. In this study, directional solidifications were carried out at various solidification rates, including $0.5{\sim}300{\mu}m/s$ in the Co base superalloy FSX-414. The cellular interface were formed at a low solidification rate, $1{\mu}m/s$, and the dendritic interface was found at higher solidification rates, $5{\sim}300{\mu}m/s$. As the spacing of dendrite structure decreased, the size and spacing of eutectics decreased. Dendrite arm spacing decreased with increasing solidification rates and temperature gradient. It was interesting to find the $M_{23}C_{6}$ eutectic microstructure formed between $\gamma$ dendrites. Composition analysis showed that Cr and W were segregated severely between the dendrites, which resulted in the formation of Cr-rich $M_{23}C_{6}$ and W-rich MC carbides.

Species Composition Dynamics and Seedling Density Along Altitudinal Gradients in Coniferous Forests of Seorak Mountain (설악산 상록침엽수림의 고도별 종조성 및 치수 밀도 변화)

  • Kim, Ji-Dong;Byeon, Seong Yeob;Song, Ju Hyeon;Chae, Seung Beom;Kim, Ho Jin;Lee, Jeong Eun;Yun, I Seul;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.109 no.2
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    • pp.115-123
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    • 2020
  • The vertical distribution of vegetation can be classified according to the altitudinal gradient and the distribution of species along this gradient. The purpose of this study was to analyze the vegetation structure, species composition, dimensional density, and change according to altitude. These data illustrate the distribution of coniferous forest by altitude. By order of importance, the vegetation structure of this mixed forest consisted of Abies nephrolepis (12.2), Pinus koraiensis (10.86), and Acer komarovii (8.11). As a result of species composition according to the altitude, A. nephrolepis and Maianthemum bifolium increased in importance with increasing altitude. Tripterygium regelii emerged between 1,400 m and 1,600 m, which indicates that forest gaps were frequent at that elevation. The species diversity index was the highest from 1,400-1,500 m and coincided with the presence of forest gaps. The changes in A. nephrolepis of evergreen conifers increased significantly from 402 ± 5.4 ha.-1 to 528 ± 11.6 ha.-1 for two years, and decreased from 57 ± 1.3 ha.-1 to 56 ± 1.6 ha.-1 for P. koraiensis. The density of A. nephrolepis and P. koraiensis seedlings significantly increased at 1,500-1,600 m. The results of this study can be used as a basis to identify the mast seeding year with the increase or decrease of seedlings. In addition to documenting the evergreen conifer population of the Seorak Mountain, these results can be built upon for future monitoring of seedlings mortality.

Impact of the Pollution on the Benthic Community Environmental impact of the pollution on the benthic coralligenous community in the Gulf of Fos, northwestern Mediterranean (북서 지중해 Fos해역의 해양오염이 해양저서생물군집 Coralligenous Community에 미치는 영향)

  • HONG Jae-Sang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.16 no.3
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    • pp.273-290
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    • 1983
  • A bionomic study of the coralligenous concretionary hard bottom in the northwestern Mediterranean was carried out at four stations : three stations(Arnette, Laurons, Auguette) under the influence of intense multisource pollution(gradient decreasing from north to south) in the Gulf of Fos, west of Marseille, France, and one control station(Moyade islet) in an unpolluted area near Riou island, east of Marseille. Along the increasing pollution gradient from the outer to the inner part of the Gulf of Fos, there is a qualitative and quantitative impoverishment of the fauna. On the whole, the species richness, the numerical abundance. and the species diversity index are all on the decrease. Accordingly, the innermost station in the Gulf of Fos(Auguette) is most heavily affected by the industrial, and to a lesser extent by the domestic wastes, from the nearby industrial complex and urban areas. The impact on the benthic coralligenous community of this serious alteration has been analysed in view of community composition, functional aspect, and ecological stocks. The faunal affinity between stations has been studied by means of the two coefficients : fourfold point correlation coefficient and Jaccard's community coefficient. The upper layer and inferior face communities of the coralligenous concretionary structures are also compared.

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Performance Characteristics of an Ensemble Machine Learning Model for Turbidity Prediction With Improved Data Imbalance (데이터 불균형 개선에 따른 탁도 예측 앙상블 머신러닝 모형의 성능 특성)

  • HyunSeok Yang;Jungsu Park
    • Ecology and Resilient Infrastructure
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    • v.10 no.4
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    • pp.107-115
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    • 2023
  • High turbidity in source water can have adverse effects on water treatment plant operations and aquatic ecosystems, necessitating turbidity management. Consequently, research aimed at predicting river turbidity continues. This study developed a multi-class classification model for prediction of turbidity using LightGBM (Light Gradient Boosting Machine), a representative ensemble machine learning algorithm. The model utilized data that was classified into four classes ranging from 1 to 4 based on turbidity, from low to high. The number of input data points used for analysis varied among classes, with 945, 763, 95, and 25 data points for classes 1 to 4, respectively. The developed model exhibited precisions of 0.85, 0.71, 0.26, and 0.30, as well as recalls of 0.82, 0.76, 0.19, and 0.60 for classes 1 to 4, respectively. The model tended to perform less effectively in the minority classes due to the limited data available for these classes. To address data imbalance, the SMOTE (Synthetic Minority Over-sampling Technique) algorithm was applied, resulting in improved model performance. For classes 1 to 4, the Precision and Recall of the improved model were 0.88, 0.71, 0.26, 0.25 and 0.79, 0.76, 0.38, 0.60, respectively. This demonstrated that alleviating data imbalance led to a significant enhancement in Recall of the model. Furthermore, to analyze the impact of differences in input data composition addressing the input data imbalance, input data was constructed with various ratios for each class, and the model performances were compared. The results indicate that an appropriate composition ratio for model input data improves the performance of the machine learning model.

Comparison of Blood and Alveolar Gas Composition during Rebreathing in the Dog Lung (반복호흡(反覆呼吸)을 하는 견폐(犬肺)에서의 혈액(血液) Gas와 폐포(肺胞) Gas의 비교(比較)에 관(關)하여)

  • Yu, Chang Jun
    • Korean Journal of Veterinary Research
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    • v.12 no.1
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    • pp.25-35
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    • 1972
  • The author used a dog preparation, in which either the left lung or the lower left lobe rebreathed continuously from a bag, to compare $CO_2$ and $O_2$ tensions in the rebreathed gas with those in blood entering or leaving the lung. In the 10 dogs studied, alveolar pressures for both gases were greater than in the blood; the "upstream" gradient (alveolar pulmonary arterial blood) exceeded the "downstream" gradient (alveolar-pulmonary venous blood) for both gases, but the difference was only significant for $CO_2$. The author attributed this effect to the production of $CO_2$ in lung tissue metabolism and its uptake by the blood. The difference in $CO_2$ tension between blood leaving and entering lung increased while the downstream gradient fell, an effect which was independant of the level of anesthesia. There was no evidence of a fall in base excess in blood passing through the lung, as would occur from the production of metabolic acids during anaerobic metabolism at the low levels found in the rebreathing lung in this preparation.

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Single crystal growth of syntheric emerald by reflux method of temperatute gradient using natural beryl (천연베릴을 이용한 온도구배 환류법에 의한 합성 Emerald 단결정 육성)

  • 최의석;김무경;안영필;서청교;안찬준;이종민
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.8 no.4
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    • pp.532-538
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    • 1998
  • Emerald ($3BeO{\cdot}Al_2O_3{\cdot}6SiO_2:Cr^{3+}$) single crystal was grown by temperature gradient reflux method with using Korean natural beryl. The flux of lithium-molibudenium-vanadium oxide system was made by means of mixing the 2 sort of flux which were differently melted $Mo_3-Li_2O$ and $V_2O_5-Li_2O$ each other. The optimum composition of flux was 3 mole ratio of molibudenium. vanadium oxides to lithium oxide ($(MoO_3+V_2O_5)/Li_2O$), flux additives were substituted more less then 0.2 mole% of $K_2O$ or $Na_2O$ to the $Li_2O$ amount. The melting concentration of mixing beryl material was 3~10% content to the flux, that of $Cr_2O_3$ color dopant was 1% to the beryl amount. In the crystal growing apparatus with temperature gradient in the 3 zone furnace which was separated into the block of melt, growth and return, the solution have got to circulate continuously between $1100^{\circ}C$ and $1000^{\circ}C$ in steady state. When thermal fluctuation was treated to during 2 hrs once on a day at 950~$1000^{\circ}C$ in growth zone, the supersaturation solution was maintained, controled and emerald single crystal can be grown large crystal which was prevented from the nucleation of microcrystallite. The preferencial growth direction of hexagonal columnar emerald single crystal was the c(0001) plane of botton side and vertical to the m(1010) plane of post side.

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Temporal variation of ecosystem carbon pools along altitudinal gradient and slope: the case of Chilimo dry afromontane natural forest, Central Highlands of Ethiopia

  • Tesfaye, Mehari A.;Gardi, Oliver;Bekele, Tesfaye;Blaser, Jurgen
    • Journal of Ecology and Environment
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    • v.43 no.2
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    • pp.161-182
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    • 2019
  • Quantifying the amount of carbon pools in forest ecosystems enables to understand about various carbon pools in the forest ecosystem. Therefore, this study was conducted in the Chilimo dry afromontane forest to estimate the amount of carbon stored. The natural forest was stratified into three forest patches based on species composition, diversity, and structure. A total of 50 permanent sample plots of 20 m × 20 m (400 ㎡ ) each were established, laid out on transects of altitudinal gradients with a distance of 100 m between plots. The plots were measured twice in 2012 and 2017. Tree, deadwood, mineral soil, forest floor, and stump data were collected in the main plots, while shrubs, saplings, herbaceous plants, and seedling data were sampled inside subplots. Soil organic carbon (SOC %) was analyzed following Walkely, while Black's procedure and bulk density were estimated following the procedure of Blake (Methods of soil analysis, 1965). Aboveground biomass was calculated using the equation of Chave et al. (Glob Chang Biol_20:3177-3190, 2014). Data analysis was made using RStudio software. To analyze equality of means, we used ANOVA for multiple comparisons among elevation classes at α = 0.05. The aboveground carbon of the natural forest ranged from 148.30 ± 115.02 for high altitude to 100.14 ± 39.93 for middle altitude, was highest at 151.35 ± 108.98 t C ha-1 for gentle slope, and was lowest at 88.01 ± 49.72 t C ha-1 for middle slope. The mean stump carbon density 2.33 ± 1.64 t C ha-1 was the highest for the middle slope, and 1.68 ± 1.21 t C ha-1 was the lowest for the steep slope range. The highest 1.44 ± 2.21 t C ha-1 deadwood carbon density was found under the middle slope range, and the lowest 0.21 ± 0.20 t C ha-1 was found under the lowest slope range. The SOCD up to 1 m depth was highest at 295.96 ± 80.45 t C ha-1 under the middle altitudinal gradient; however, it was lowest at 206.40 ± 65.59 t C ha-1 under the lower altitudinal gradient. The mean ecosystem carbon stock density of the sampled plots in natural forests ranged from 221.89 to 819.44 t C ha-1. There was a temporal variation in carbon pools along environmental and social factors. The highest carbon pool was contributed by SOC. We recommend forest carbon-related awareness creation for local people, and promotion of the local knowledge can be regarded as a possible option for sustainable forest management.

Seasonal Dynamics of Fish Fauna and Compositions in the Gap Stream Along With Conventional Water Quality

  • Lee, Jae-Hoon;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.40 no.4
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    • pp.503-510
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    • 2007
  • The purposes of the study were to analyze the seasonal effects on the fish fauna and compositions including trophic guilds and tolerance guilds. For the study, we collected fish samples twice in June as premonsoon period and early September 2007 as monsoon periods in five sampling sites of the Gap Stream, and then biological oxygen demand (BOD), nutrients (TN, TP) and suspended solids (SS) were compared with the guild data along the gradient of upstream-to-downstream. Chemical water quality, based on BOD, TP, and TN degraded gradually from the upstream to downstream reach and there were about 3 fold difference between S1 and S5. Water quality was worse in the premonsoon than the monsoon, and the heavy monsoon resulted in a dilution of the polluted river by rain water, especially, in the downstream reach. Total number of fish species, based on the catch per unit effort (CPUE), showed a distinct difference between the two seasons; 30 species were sampled in premonsoon, but 23 species were sampled in the monsoon, indicating a seasonal difference in the fish fauna. Tolerant species dominated the fish community (48.3%) in the stream, and the proportions prior to physical disturbance by the monsoon rain were evidently greater in the downstream reach than the upstream. This reflected the characteristics of urban stream polluted by nutrient enrichment as shown in the BOD and TP values. Sensitive species in the premonsoon decreased from the gradient of upstream-to-downstream reach. Such seasonal modifications in the trophic and tolerance guilds were evident. In the analysis of trophic guild and habitat guild, during the premonsoon the proportion of insectivore and riffle-benthic species were largely greater in the upstream reach than the downstream, whereas the proportions were opposite along the gradient of the stream in monsoon. Thus, the patterns of chemical water quality along the longitudinal gradients reflected the premonsoon conditions of insectivores and tolerant species, indicating that summer monsoon data of fish may not match with water quality due to large physical disturbance by flow regime. Seasonal monsoon in this region as well as the chemical pollution may act as a key role influencing the fish compositions of trophic and tolerance guilds and fauna. The data collected during the premonsoon rather than the monsoon, thus, may be better predictor for a diagnosis of stream health conditions.