• Bulletin of the Korean Chemical Society
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• 제22권1호
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• pp.46-52
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• 2001

To evaluate the non-ionic polymer, poly(ethylene glycol) (PEG), as a component in cationic copolymers for non-viral gene delivery systems, PEG was coupled to polyethylenimine (PEI). We present the effects of different degrees and shapes of pegylation of PEI on cytotoxicity, water solubility and transfection efficiency. This work reports the synthesis and characterization of a series of cationic copolymers on the basis of the conjugates of PEI with PEG. The modified molecules were significantly less toxic than the original polymer. Moreover, the chemical modification led to enhancement of their solubility. The comparison of pegylated PEIs with different degrees of derivation showed that all the polymers tested reached comparable levels of transgene expression to that of native PEI. As assessed by agarose gel electrophoresis, even highly substituted PEI derivatives were still able to form polyionic complexes with DNA. However, aside from an increase in solubility and retention of the ability to condense DNA, methoxy-PEG-modified PEIs resulted in a significant decrease in the transfection activity of the DNA complexes. In fact, the efficiency of the copolymer was compromised even at a low degree of modification suggesting that the PEG action resulting from its shape is important for efficient gene transfer. The mode of PEG grafting and the degree of modification influenced the transfection efficiency of PEI.

• 한국기계가공학회지
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• 제18권8호
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• pp.8-17
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• 2019

In this study, a model reduction technique was used to develop a precise noise and vibration prediction model for the individual components of a driveline system. The dynamic reduced-order model generated by the Craig-Bampton method was applied to perform dynamic analysis of an electric agricultural power cart. The natural frequency and acceleration response results were analyzed according to the different number of dominant sub-structural modes contained in the reduced-order models. Through the analysis results, it was confirmed that a sufficient number of dominant sub-structures to satisfy the operating conditions should be selected to construct an optimal reduced-order model.

• 한국항공우주학회지
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• 제30권4호
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• pp.106-113
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• 2002

인공위성에서 위성 구조체의 임무는 위성체의 모든 부품을 제반 환경조건 하에서 안전하게 지지하는 것이다. 위성 구조체의 안전성은 위성체 및 발사체 모델의 결합 및 하중함수로 표현되는 발사하중을 부가한 연성하중해석을 통해 최종적으로 검증된다. 본 연구에서는 구조체 무게의 감소를 위해, 발사하중상태 하에의 위성구조에 대해 직접 최적화알고리듬을 적용하였다. 위성 구조부재의 손상여부의 판단을 위한 가속도 반응은 연성하중해석의 결과를 바탕으로 얻었다. 최적화 결과, 구조부재 중량은 약 12%의 감소를 보였으며, 하니콤 심재의 두께가 성능에 크게 기여함을 알 수 있었다.

• 한국전산구조공학회논문집
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• 제35권3호
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• pp.175-182
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• 2022

부분구조화 기법은 자유도가 많고 복잡한 구조물의 유한요소 해석 모델 단순화에 효율적으로 적용될 수 있는 기법이다. 대표적으로 선형 문제에 대해서는 Craig-Bampton method 등이 있다. Craig-Bampton method는 경계 요소를 제외한 나머지 요소의 불필요한 자유도를 제거함으로써 선형 구조물의 축소를 수행한다. 최근에는 부분구조화 기법과 더불어 구조물의 최적설계를 위해 멀티레벨 최적화 기법이 많이 활용되고 있다. 시스템의 목표를 달성하기 위해 각 부구조에 새로운 목표를 할당하는 기법이다. 본 연구에서는 유전자 알고리즘을 이용하여 시스템 목표 달성을 위한 각 부구조별 내부 자유도 개수를 새로운 목표로 할당하고 최적화를 수행하였다. 최적화 절차로부터 도출된 부구조별 내부 자유도 개수를 이용하여 시스템의 축소를 수행하였다. 다양한 수치예제들을 통해 축소 모델에 대한 결과를 확인하였으며, 90% 이상의 정확도를 가지는 것을 확인하였다.

• Tribology and Lubricants
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• 제36권2호
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• pp.105-115
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• 2020

This paper presents a numerical study on the rotordynamic analysis of a dual-spool turbofan engine in the context of blade defect events. The blades of an axial-type aeroengine are typically well aligned during the compressor and turbine stages. However, they are sometimes exposed to damage, partially or entirely, for several operational reasons, such as cracks due to foreign objects, burns from the combustion gas, and corrosion due to oxygen in the air. Herein, we designed a dual-spool rotor using the commercial 3D modeling software CATIA to simulate blade defects in the turbofan engine. We utilized the rotordynamic parameters to create two finite element Euler-Bernoulli beam models connected by means of an inter-rotor bearing. We then applied the unbalanced forces induced by the mass eccentricities of the blades to the following selected scenarios: 1) fully balanced, 2) crack in the low-pressure compressor (LPC) and high pressure compressor (HPC), 3) burn on the high-pressure turbine (HPT) and low pressure compressor, 4) corrosion of the LPC, and 5) corrosion of the HPC. Additionally, we obtained the transient and steady-state responses of the overall rotor nodes using the Runge-Kutta numerical integration method, and employed model reduction techniques such as component mode synthesis to enhance the computational efficiency of the process. The simulation results indicate that the high-vibration status of the rotor commences beyond 10,000 rpm, which is identified as the first critical speed of the lower speed rotor. Moreover, we monitored the unbalanced stages near the inter-rotor bearing, which prominently influences the overall rotordynamic status, and the corrosion of the HPC to prevent further instability. The high-speed range operation (>13,000 rpm) coupled with HPC/HPT blade defects possibly presents a rotor-case contact problem that can lead to catastrophic failure.