• Wind and Structures
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• v.20 no.5
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• pp.661-682
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• 2015

This study investigates the use of time-frequency coherence analysis for detecting and evaluating coherent "structures" of surface pressures and wind turbulence components, simultaneously on the time-frequency plane. The continuous wavelet transform-based coherence is employed in this time-frequency examination since it enables multi-resolution analysis of non-stationary signals. The wavelet coherence quantity is used to identify highly coherent "events" and the "coherent structure" of both wind turbulence components and surface pressures on rectangular prisms, which are measured experimentally. The study also examines, by proposing a "modified" complex Morlet wavelet function, the influence of the time-frequency resolution and wavelet parameters (i.e., central frequency and bandwidth) on the wavelet coherence of the surface pressures. It is found that the time-frequency resolution may significantly affect the accuracy of the time-frequency coherence; the selection of the central frequency in the modified complex Morlet wavelet is the key parameter for the time-frequency resolution analysis. Furthermore, the concepts of time-averaged wavelet coherence and wavelet coherence ridge are used to better investigate the time-frequency coherence, the coherently dominant events and the time-varying coherence distribution. Experimental data derived from physical measurements of turbulent flow and surface pressures on rectangular prisms with slenderness ratios B/D=1:1 and B/D=5:1, are analyzed.

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.5
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• pp.1300-1310
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• 1990

Naphthalene sublimation technique is employed to investigate the mass transfer processes from a square cylinder at various Reynolds numbers and various angles of attack. Distribution of the local mass transfer coefficients on each face of the cylinder changes dramatically with the angle of attack. Such variation of local mass transfer rates closely connected with the complex flow phenomena such as stagnation, acceleration, separation, reattachment and vortex shedding. The average Sherwood number has a minimum value at 12.deg.-13.deg., and a maximum value at a=20.deg.-25.deg. A comparison of present mass transfer measurement with other heat transfer measurements, using the heat/mass transfer analogy, shows good agreement in average transfer rates, same trend but notable differences in local values. Therefore, naphthalene sublimation technique can be adopted to explore heat transfer processes in the complex flow situations, which is considered to be hardly possible with the conventional heat transfer measurements.

• Bulletin of the Korean Chemical Society
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• v.25 no.8
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• pp.1137-1142
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• 2004

A spectrophotometric and first derivative spectrophotometric method was developed in aquatic Tween 80 micellar solutions for selective determination of nickel without using any pre-separation step. 1-(2-Pyridylazo)-2-naphthol (PAN), as a sensitive chromogenic complexing agent formed a red-colored Ni(II)-PAN complex in Tween 80 media with satisfactory solubility and stability. Conditions such as pH, PAN concentration, type and concentration of micellizing agent were optimized. Molar absorptivity of Ni-PAN complex was found $4.62\;{\times}\;10^4L\;cm^{?1}\;mol^{?1}$ at 569 nm, under the optimum condition. Calibration graphs were derived by zero, first and second derivative spectrophotometry at maximum wavelengths of 569, 578 and 571 nm with linear ranges of 30-1800, 20-2500 and 30-2000 ng $mL^{?1}$ , respectively. Precision as standard deviation as well as accuracy as recovery percent were in the range of 1-20 ng $mL^{?1}$, and 93.3-103.3%, respectively, for the entire of the linear ranges. Spectrophotometric detection limit was 3 ng $mL^{?1}$ and effects of diverse ions on the first derivative determination of nickel were studied to investigate selectivity of the method. Interferences of cobalt and copper on the nickel determination were prevented using o-phenanthroline as masking agent. The recommended procedures were applied to the various synthetic and stainless steel alloys, tea leaves and human hair, with satisfactory results.

• KSBB Journal
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• v.9 no.2
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• pp.115-121
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• 1994

A Purification device with 30-channel free-flow electrophoresis was assembled to treat samples of 240m1 volume for purification of lentil lectin (LcH) from lentil seeds with no impurities in a silverstained PAGIEF gel. HEPES(50mM)-Ttis(50mM), Cycloserine(50mM)-urea(3M), Histidine(50mM)-urea(3M) were used as ampholytea among which Histidine(50mM)-urea(3M) (pI 7.65) was found best in resolution. LcH is known to be present in the form of LcH-A, LcH-B and the complex of the two. The complex, however, disappeared when urea was added in the ampholytes, which suggested that the complete purification of two isolectins is possible using the present multistep purificaton device.

• Journal of the Korean Chemical Society
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• v.34 no.1
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• pp.76-84
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• 1990

Optical isomers of dansyl amino acids were separated by a chiral mobile phase addition method. Two metha and para isomers of xylenyl-L-proline were prepared and used as the ligands of copper(Ⅱ) chelate to resolve the dansyl amino acids. Their elution behaviors were similar to those obtained from the addition of copper (Ⅱ) benzyl-L-proline chelate. The matrix effect of the mobile phase such as pH, concentration of buffer and compositions of organic solvent acetonitrile affected the optical resolution. The separation mechanism could be explained by a cis-trans effect of the ligand exchange reaction and hydrophobic interaction between the ternary complex and the stationary phase.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2017.11a
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• pp.118-119
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• 2017

The combined waterproofing method is an excellent method to overcome the disadvantages of the single waterproofing method by composing two or more materials to complement each other, but it is a method that can cause defects such as separation and peeling between materials due to the heterogeneity of the applied two materials. In order to improve this, in this study, we aimed to develop a technology for inducing chemical unification between materials through a urea reaction with a coating material applied on the lower side by laminating a nonwoven fabric treated with a modified amine on the back surface of the sheet material, The adhesion performance test was carried out with the presence or absence of denatured amine treated nonwovens as variables. As a result of the test, it was confirmed that the adhesion performance of the specimen to which the modified amin - treated nonwoven fabric was applied was improved by about 60% or more as compared with the specimens not having the denatured amine treated specimen.

• Journal of Life Science
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• v.7 no.2
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• pp.79-87
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• 1997

To determine the cause of Vibrio septicemia by understanding the characteristics endotoxin from Vibrio vulnificus, lethal dose, heat resistance and vascular permeability enhancing activity were svaluated using vegestative cell and cell homogenate and the result is as follows: 1. Vibrio vulnificus CDC B3547 of patient origin did not exihibit any significant difference in toxicity compared to Vibrio vulnificus B57 of enviroment origin. 2. Strong toxicity was observed when viable cell count of Vibrio vulnificus CDC B3547 was more than 10$^{7}$/ml. 3. Toxicity of cell homogenate was completely inactivited upon geating at 80$^{\circ}$C for 20min. 4. Cell homogenate did not show hemolyic activity but was acknowleged to have cytotoxicity. 5. Major lethal toxin against mouse was existed in Vibrio vulnificus CDC B3547; however, separation of LPS and LPS-protein complex was not successful using the current technique.

• Analytical Science and Technology
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• v.32 no.2
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• pp.35-47
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• 2019

In this study, high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to detect 26 antidiabetic compounds in adulterated dietary supplements using a simple, selective method. The work presented herein may help prevent incidents related to food adulteration and restrict the illegal food market. The best separation was obtained on a Shiseido Capcell Pak(R) C18 MG-II ($2.0mm{\times}100mm$, $3{\mu}m$), which improved the peak shape and MS detection sensitivity of the target compounds. A gradient elution system composed of 0.1 % (v/v) formic acid in distilled water and methanol at a flow rate of 0.3 mL/min for 18 min was utilized. A triple quadrupole mass spectrometer with an electrospray ionization source operated in the positive or negative mode was employed as the detector. The developed method was validated as follows: specificity was confirmed in the multiple reaction monitoring mode using the precursor and product ion pairs. For solid samples, LOD ranged from 0.16 to 20.00 ng/mL and LOQ ranged from 0.50 to 60.00 ng/mL, and for liquid samples, LOD ranged from 0.16 to 20.00 ng/mL and LOQ ranged from 0.50 to 60.00 ng/mL. Satisfactory linearity was obtained from calibration curves, with $R^2$ > 0.99. Both intra and inter-day precision were less than 13.19 %. Accuracies ranged from 80.69 to 118.81 % (intra/inter-day), with a stability of less than 14.88 %. Mean recovery was found to be 80.6-119.0 % and less than 13.4 % RSD. Using the validated method, glibenclamide and pioglitazone were simultaneously determined in one capsule at concentrations of 1.52 and 0.53 mg (per capsule), respectively.

• Journal of Korean Elementary Science Education
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• v.43 no.1
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• pp.122-135
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• 2024

The purpose of this study was to analyze the examples presented in the "Separation of Mixtures" section of the 2015 revised science authorized textbook introduced in elementary schools in 2022 and to see how the teachers and students understand the concept. To do that, 96 keywords were extracted through three cleansing processes to separate the elements of the mixture presented in the textbook. In order to analyze the teachers' perceptions, 32 teachers at elementary schools in Gyeonggi-do received responses to a survey, and a survey of 92 fourth graders who learned the separation of the mixture with an authorized textbook in 2022 was used for the analysis. As a result, as for the solids, 54 out of 96 separations (56.3%) showed the highest ratio, and the largest number of cases were presented according to the characteristics of the development stage of students. It was followed by living things, liquids, other objects and substances, and gasses. By analyzing the mixture, the structure and the interrelationships between the 96 extracted keywords were systematized through the network analysis, and the connection between the keywords, which were a part of the mixture was analyzed. The teachers partially responded to the separation of the complex mixture presented in the textbook, but most of the students did not recognize it. Because the analysis of the teachers' and students' perceptions of the seven separate categories presented in the survey was not based on a clear conceptual perception of the separation of the mixture, but rather they tended to respond differently for each characteristic of each individual category, it was decided that it was necessary to present clearer examples of the separation of the mixture, so that the students could better understand the concept of separation of mixtures that could be somewhat abstract.

• Journal of the Korean Ceramic Society
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• v.25 no.3
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• pp.251-260
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• 1988

Ion-exchange porous glasses were prepared by heat treatment and subsequently hydro thermalor acid leaching treatment $10Li_2O$.$(90-x)B_2O_3$.$xSiO_2$ base glasses containing various amount of $Al_2O_3$ or $MoO_3$. It was investigated how the phase separation and the cation exchange capacity(CEC) were affected by the addition of $Al_2O_3$ or $MoO_3$. The optimum condition of phase separation in these glasses was about 48$0^{\circ}C$ for 10 hrs. The degree of phase separation was rapidly suppressed by the addition of $Al_2O_3$ up to 10 mol% and thereafter suppression effect was decreased. The maximum value of CEC, about 252meq/100g, was observed with the $1OLi_2O$.$45B_2O_3$.$45SiO_2＋7.5Al_2O_3$ porous glass prepared by hydrothermal treatment and its mean pore radius was about 16.3A. The addition of $MoO_3$ accelerated phase separation and leaching rate. Looking at the remakable increment of pore diameter and pore volume of these porous glasses by the addition of $MoO_3$, the effect of $MoO_3$ may be ascribed to the lowering of silica concentration in the borate phase and to the forming of water-soluble complex with silica during the leaching treatment.