• Biomolecules & Therapeutics
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• 제29권5호
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• pp.527-535
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• 2021

Sclerostin (SOST), a regulator of bone formation in osteocytes, inhibits the canonical Wnt signaling by interacting with low-density lipoprotein receptor-related protein 5/6 (LRP5/6) to prevent Wnt binding. Loss-of-function mutations of the SOST gene caused massive bone outgrowth and SOST-null mouse exhibited a high bone density phenotype. Therefore, SOST has been suggested as a promising therapeutic target for osteoporosis. A few previous studies with X-ray crystallography identified the binding interfaces between LRP6 and SOST, but there are limitations in these studies as they used truncated SOST protein or SOST peptide. Here, we analyzed the conformational dynamics of SOST-LRP6 E1E2 complex using hydrogen/deuterium exchange mass spectrometry (HDX-MS). We examined the effect of the C-terminal tail of SOST on LRP6 conformation upon complex formation. HDX-MS analysis suggested a new potential binding interface for the C-terminal region of SOST that was missing from the previous crystal structure of the SOST-LRP6 E1E2 complex.

• 제어로봇시스템학회논문지
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• 제14권11호
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• pp.1103-1109
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• 2008

Since the flexible joint robots with motor dynamics are represented by the fifth-order nonlinear sγstem, it is difficult and complex to design the controller for electrically driven flexible-joint (EDFJ) robots. In this paper, we propose a simple adaptive control method to solve this problem. It is assumed that the model uncertainties of the robots dynamics, joint flexibility, and motor dynamics are unknown. For the simple control design, the dynamic surface design method is applied, and all uncertainties in the robot and motor dynamics are compensated by using the adaptive function approximation technique. It is proved that all signals in the controlled closed-loop system are uniformly ultimately bounded. Simulation results for three-link EDFJ manipulators are provided to validate the effectiveness of the proposed control system.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2010년 춘계학술대회논문집
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• pp.517-523
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• 2010

Recently, the rapid evolution of computational fluid dynamics (CFD) has enabled its key role in industries and predictive sciences. From diverse research disciplines, however, are there strong needs for integrated analytical tools for multi-phenomena beyond simple flow simulation. Based on the concurrent simulation of multi-dynamics, multi-phenomena beyond simple flow simulation. Based on the concurrent simulation of multi-dynamics, multi-physics and multi-scale phenomena, the multi-phenomena CFD technology enables us to perform the flow simulation for integrated and complex systems. From the multi-phenomena CFD analysis, the high-precision analytical and predictive capacity can enhance the fast development of industrial technologies. It is also expected to further enhance the applicability of the simulation technique to medical and bio technology, new and renewable energy, nanotechnology, and scientific computing, among others.

• 한국시스템다이내믹스연구
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• 제12권1호
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• pp.115-130
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• 2011

This study shows the operational availability(Ao) analysis of the supply chain with maintenance functions using the system dynamics simulation. The simulation uses 60 equipments which are serial systems composed by 4 major components. And every entities are connected each other by causal loops. So whole simulation executed like one organic system. Specially we consider 2 constraints, one is the number of spare parts and the other is maintenance capacity level. 2 constraints have 11 levels each so the simulation has 121(11*11) scenarios which scenario has 30 different random number seed. The simulation executed total 3,630(11*11*30) times. We analysis average Ao of total equipments by 121 scenarios and additionally the regression of the average Ao and 2 constraints. As the result, we can get the more accurate values by the system dynamics simulation than the regression to analysis complex system like the supply chain with maintenance functions.

• 한국시스템다이내믹스연구
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• 제9권1호
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• pp.199-218
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• 2008

Empirical studies have shown that government and human service nonprofit organizations have maintained active partnerships to deliver human services for the past several decades. However, the previous researches on resource dependent government-nonprofit service delivery have revealed serious methodological limitations to adequately demonstrate the dynamic relationship between both sectors over time. Since the dynamics underlying the government-nonprofit service delivery is a complex process, in which multiple factors dynamically interact over time, the assumptions of system dynamics can help improve these methodological drawbacks. This study aims to explore methodological issues and weaknesses observed in empirical studies on resource dependent government-nonprofit service delivery in the United States, and further attempts to provide insights on future research toward a dynamic approach, bringing the assumptions of system dynamics to the challenges of the previous researches.

• 한국시스템다이내믹스연구
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• 제6권1호
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• pp.147-176
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• 2005

This paper attempts to analyse the financial validity of the Eco-therapy project which 'J' city in 'C' province has been eagerly trying to implement for its regional economic development and also to estimate the potential economic impact of the project on its regional economy. In doing so, it employs system dynamics which is a methodology for studying and managing complex systems, such as one finds in business and other social systems. Analytic framework for this study is constructed, based on the existing theoretical studies. This study concludes that the Eco-therapy Project concerned is economically and financially valid and it is also expected that the regional economic impacts arising from the construction and operation of the facilities concerned are greater than any other project which 'J' city has been pursuing.

• BMB Reports
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• 제34권6호
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• pp.551-558
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• 2001

We extended the measurable time scale of DNA dynamics to submicrosecond using a long-lifetime metal-ligand complex, $[Ru(phen)_2(dppz)]^{2+}$ (phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuPD), which displays a mean lifetime near 350 ns. We partially characterized the fluorescence resonance energy transfer (FRET) in calf thymus DNA from RuPD to nile blue (NB) using frequency-domain fluorometry with a high-intensity, blue light-emitting diode (LED) as the modulated light source. There was a significant overlap of the emission spectrum of the donor RuPD with the absorption spectrum of the acceptor NB. The F$\ddot{o}$rster distance ($R_0$) that was calculated from the spectral overlap was $33.4\;{\AA}$. We observed dramatic decreases in the steady-state fluorescence intensities of RuPD when the NB concentration was increased. The intensity decays of RuPD were matched the closest by a triple exponential decay. The mean decay time of RuPD in the absence of the acceptor NB was 350.7 ns. In a concentration-dependent manner, RuPD showed rapid intensity decay times upon adding NB. The mean decay time decreased to 184.6 ns at $100\;{\mu}M$ NB. The FRET efficiency values that are calculated from the mean decay times increased from 0.107 at $20\;{\mu}M$ NB to 0.474 at $100\;{\mu}M$ NB concentration. The use of FRET with a long-lifetime metal-ligand complex donor is expected to offer the opportunity to increase the information about the structure and dynamics of nucleic acids.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.356.3-357
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• 2002

Metoprolol (MT) is one kinds of adrenergic beta-blockers. Its (S)-enantiomer is known to be more active than the (R). Recently. the x-ray structure of beta-blocker. (S)-propranolol (a-naphthyl analogue), complexed in a mould fungal cellulase. Cel7A. was reported and the (R)-form did not build any complex. And in our previous study the conformation and stability of MT in carboxymethylated beta-cyclodextrin (BCD) complex was determined by NMR. HPLC, UV and electrophoresis measurement. (omitted)

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1993년도 한국자동제어학술회의논문집(국내학술편); Seoul National University, Seoul; 20-22 Oct. 1993
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• pp.742-748
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• 1993

The dynamics and control of two complex column configurations (sidestream column with stripper; prcfractionater/sidestream column configuration), which are multivariable interacting and nonlinear, have been studied. A new control scheme developed by Hanand Park(1993) to deal with the nonlinear and multivariable nature of distillation processes has been applied to these complex distillation configurations. The control scheme incorporates a nonlinear wave model into a generic model control framework. An observer based on the nonlinear wave model is used to determine the profile positions of distillation column sections. The control scheme enables tight control of the profile position of each column section that leads to fast stabilization of product compositions.

• Journal of Pharmaceutical Investigation
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• 제21권4호
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• pp.223-230
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• 1991

The structural specificity and the chemical dynamics between ${\beta}-cyclodextrin$ and aspirin were studied by FT-IR UN, $^1H$ NMR. $^{13}C NMR$. and FAB-MS spectroscopy in solution and solid state, A stable solid inclusion complex was prepared by the recrystallization method, From the spectral changes of the host and guest molecules, orientational preference for binding in the cyclodextrin cavity was determined.