• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.33-34
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• 2006

The graft copolymer consisting of poly(N-isopropylacrylamide) (PNIPAAm) and single-stranded DNA was prepared. Interestingly, the copolymer was found to form nanoparticles above physiological temperature. We found that non-crosslinking aggregation of the nanoparticles was induced by the hybridization of the surface-bound DNA with the full-match complementary DNA, but not with one-base mismatch. The core material is not restricted to PNIPAAm; DNA-functionalized gold nanoparticle was found to show a similar aggregation induced only by the fully-complementary DNA, resulting in rapid color change within 3 min at ambient temperature. This methodology is general in principle and applicable for wide variety of clinical gene diagnosis.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.979-982
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• 2006

Two knockout vectors, in which the truncated Kluyveromyces lactis URAS gene is flanked by a direct repeat, were developed for Saccharomyces cerevisiae. Each vector was designed to harbor 5'- and 3'-end homology regions for integration. Two knockout fragments were devised to integrate into the correct locus in a complementary manner to disrupt a gene of interest and. concomitantly to make functional Kl URA3 for transfomant selection. The use of dual complementary knockout cassettes was expected to dramatically reduce integration into unwanted loci in the genome. The knockout system developed in this study was successfully used for disruption of the GAL1 gene in S. cerevisiae.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2004년도 춘계학술대회 학술발표 논문집 제14권 제1호
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• pp.97-100
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• 2004

The identification of the DNA structure as a double-stranded helix consting of two nucleotide chain molecules was a milestone in modern molecular biology. The DNA chip technology is based on reverse hybridization that follows the principle of complementary binding of double-stranded DNA. DNA chip can be described as the deposition of defined nucleic acid sequences, probes, on a solid substrate to form a regular array of elements that are available for hybridization to complementary nucleic acids, targets. DNA chips based on cDNA clons, oligonucleotides and genomic clons have been developed for gene expression studies, genetic variation analysis and genomic changes associated with disease including cancers and genetic diseases. DNA chips for gene expression profiling can be used for functional analysis in human eel Is and animal models, disease-related gene studies, assessment of gene therapy, assessment of genetically modified food, and research for drug discovery. DNA chips for genetic variation detection can be used for the detection of mutations or chromosomal abnormalities in cnacers, drug resistances in cancer cells or pathogenic microbes, histocompatibility analysis for transplantation, individual identification for forensic medicine, and detection and discrimination of pathogenic microbes. The DNA chip will be generalized as a useful tool in clinical diagnostics in near future. Lab-on-a chip and informatics will facilitate the development of a variety of DNA chips for diagnostic purpose.

• 한국양식학회지
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• 제16권2호
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• pp.124-127
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• 2003

Our previous studies of both microarray analysis in channel catfish muscle gene expression of 2 different ages and channel catfish muscle expressed sequence tag profiles demonstrated parvalbumin beta is one of the highly expressed muscle transcriptome. We have cloned and sequenced complementary DNA encoding the channel catfish parvalbumin which encode 109 amino acids. The deduced amino acid sequences of the catfish parvalbumin are highly conserved with those cloned from other teleosts. The availability of the catfish parvalbumin provides the opportunity of studying fish epitopes.

• 한국식물병리학회지
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• 제11권1호
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• pp.87-90
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• 1995

Complementary DNA of the movement protein (MP) gene of tobacco mosaic virus Korean pepper strain (TMV-KP) was synthesized from purified TMV-KP RNA by using the reverse transcription and polymerase chain reaction (PCR) system. The synthesized double stranded cDNA was cloned into the plasmid pUC9 and transformed into Escherichia coli JM110. The movement protein gene of TMV-KP of the selected clones was subjected to sequence analysis by Sanger's dideoxy chain termination method. The complete sequence of viral MP gene from TMV-KP strain was 807 nucleotides long. The nucleotide of MP gene from TMV-KP has thirteen and two nucleotide differences from TMV vulgarae (TMV-OM) and Korean (TMV-K) strains, respectively. Thus, the nucleotide sequence of TMV-KP MP gene showed higher homology of 99% with that of TMV-K MP gene.

• 한국작물학회지
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• 제21권1호
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• pp.52-56
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• 1976

한국의 주요품종들이 가지고 있는 엽초자색의 발현에 관여하는 보족유전자 C 및 A유전자의 분포를 조사하기 위하여 한국의 현재 및 과거장려품종과 약간의 도입품종 합계 60품종을 네가지 검정종(0군=Furisaka＃5, C군=T(N)1, A군=7089 및 CP-SLO, CA군=BPI-76)과 교배하여 그 F$_1$의 발색을 조사하였다. 그 결과를 요약하면 다음과 같다. 1. 현장려품종들은 C 및 A검정종에 모두 반응이 없는 O군과 C검정종에만 반응하는 A군으로 분명히 나눌 수 있었다. Japonica 모본으로 육성된 품종들은 전자에, Indica모본으로 육성된 품종들은 후자에 속한다. 2. 과거장려품종들은 대부분이 O군에 속하며 A검정종에 대하여 반응을 나타내는 C군품종이 극소수있었고(수원118호, 수원8002) A품종군에 속하는 것은 없었다. 3. 일본에서 도입된 품종들도 대체로 한국에서 육성된 품종들과 같이 대부분이 O군에 속하여 극소수의 C군품종이 있었을 뿐 A군품종은 없었다. 4. 과거의 찰품종도 메품종과 같이 대부분이 O군에, 그리고 극소수가 C군에 속하며 A군품종은 없었다.

• Journal of Animal Science and Technology
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• 제46권4호
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• pp.555-562
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• 2004

본 연구는 목저은 다음과 같다: 1) 돼지에서 150-kDa temary insulin-like growth faetor(IGF)complex의 한 구성 요소인 acid-labile subunit(ALS) 유전자 intron의 존재 확인. cloning 및 돼지 ALS(porcine ALS; pALS) complementary DNA(cDNA)의 3' 비해독(untranslated) 부위(3' UT) 증폭. cloning, 2) intron-spanning primer pair를 이용한 reverse transcription-polymerase chain reaction(RT-PCR) 방법에 의한 돼지 조직에서의 ALS 유전자 발현 분포 확인 및 3) 돼지 hepatocyte에서의 ALS 유전자 발현 여부 확인. 돼지 genomic DNA를 template로 하여 PCR 방법으로 예상된는 intron 부위를 증폭하고 plasmid vector에 삽입하여 염기서열을 결정한 결과 타 종의 ALS 유전자에서와 같은 위치에 1,371-base pair(bp)의 pALS intron이 존재함을 확인하였다. 역시 본 연구에서 간에서 추출한 RNA를 주형으로 시작하여 3' rapid amplification of cDNA end(3' RACE) 방법으로 147-bp의 3'UT를 합성하고 그 염기성열을 결정하였다. RT-PCR 결과 간은 물론 조사된 모든 돼지의 내장기관(신장, 폐, 비장)과 자성 생식기관(난소, 난관, 자궁) 및 골격근육에서 ALS 유전자가 발현됨이 밝혀졌다. 또한 돼지 간 조직에 대한 in-situ hybridization 결과 hepatocyte에서 ALS 유전자가 발현됨이 확인되었다. 이상의 결과는 ALS가 혈중 IGF의 저정/조절체로서의 주기능 외에 모세혈관 밖에서도 미지의 기능이 있을 기능성을 시사한다.

• 식물조직배양학회지
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• 제28권3호
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• pp.147-151
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• 2001

Explants of Lactuce sativa cultivar, chungchima, were co-cultivated with Agrobacterium tumefaciences LBA4404, EHA101 strains containing nptll gene and ferritin gene encoding iron storage protein from soybean for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 MS basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were tested with PCR analysis using nptll, ferritin specific primers whether ferritin gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot showed that transcripts of ferritin gene were detected in mature leaf of the transgenic lines. These results suggest that ferritin gene be successfully integrated and transcribed in the putative transgenic lettuce plants.

• ALGAE
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• 제38권2호
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• pp.93-110
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• 2023

Progress in phylogenomic analysis has led to a considerable re-evaluation of former cyanobacterial system, with many new taxa being established at different nomenclatural levels. The family Geminocystaceae is among cyanobacterial taxa recently described on the basis of polyphasic approach. Within this family, there are six genera: Geminocystis, Cyanobacterium, Geminobacterium, Annamia, Picocyanobacterium, and Microcrocis. The genus Geminocystis previously encompassed two species: G. herdmanii and G. papuanica. Herein, a new species G. urbisnovae was proposed under the provision of the International Code of Nomenclature for algae, fungi, and plants (ICN). Polyphasic analysis was performed for five strains from the CALU culture collection (St. Petersburg State University, Russian Federation), and they were assigned to the genus Geminocystis in accordance with high 16S rRNA gene similarity to existing species, as well as because of proximity to these species on the phylogenetic trees reconstructed with RaxML and Bayes methods. Plausibility of their assignment to a separate species of the genus Geminocystis was substantiated with smaller cell size; stenohaline freshwater ecotype; capability to complementary chromatic adaptation of second type (CA2); distinct 16S rRNA gene clustering; sequences and folding of D1-D1' and B box domains of the 16S-23S internal transcribed spacer region. The second objective pursued by this communication was to provide a survey of the family Geminocystaceae. The overall assessment was that, despite attention of many researchers, this cyanobacterial family has been understudied and, especially in the case of the crucially important genus Cyanobacterium, taxonomically problematic.

• 생명과학회지
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• 제28권4호
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• pp.398-407
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• 2018

벼 자색 종자과피(Purple pericarp, Prp) 형질은 주요 생리활성물질인 안토시아닌 C3G 생성에 관여하며 흑미를 결정하는 주요 유전형질이다. Prp 유전형질을 가진 흑미와 종자과피에 색이 없는 벼를 교배할 경우 그 후대는 검정색, 갈색, 백색이 각각 9:3:4로 분리된다. 1921년 Nagai에 의하여 제시된 바 벼 종자 색의 9:3:4 유전분리비로 인하여 벼 Prp 형질은 유전자의 열성상위(recessive epistasis) 현상으로 해석되었다. 그러나 흑미를 결정하는 Prp 형질은 두 개의 상보적 유전자들의 상호관계(complementary gene interaction)에 의한 것이기도 하다. 본 연구에서는 이러한 논란이 발생되는 이유를 설명하기 위하여 두 유전자의 조성이 완전한 이형접합인 Pbpb Pppp 유전자형을 가진 $F_1$ 잡종을 만들었다. 이들의 자손은 진한자색(검정), 중간자색, 갈색, 백색 종자이며 각각 3:6:3:4로 분리되었다. 즉, 검정색, 갈색, 백색의 종자가 각각 9:3:4의 비율로 분리된다. 그러나 생화학적인 분석결과 이들은 안토시아닌 중 cyanidin 3-O-glucoside (C3G)가 축적된 검정색 종자와 C3G가 없는 갈색 또는 백색 종자인 두 개의 집단을 분리되며 정확히 9:7의 분리 비를 갖는다. 이 경우 벼 Prp 형질을 갖는 검정쌀 또는 흑미는 전형적인 상보적 유전자의 상호관계에 의한 유전현상이다. 즉, 흑미의 자색 종피 형질 발현에는 Pb 유전자와 Pp 유전자에서 각각 한 개 이상의 우성대립인자의 발현이 필요하다. 그러나 Pb 유전자만 우성대립인자가 존재하는 Pb_ pppp 유전자형의 벼는 C3G를 생성하지 못하고 갈색 종자과피(Brown pericarp, Brp) 형질을 갖게 된다. 즉 갈색쌀는 우성 Pb 유전자의 우성대립인자에 의하여 결정된다. 그러므로 종피색을 결정하는 Prp 형질의 유전양상은 열성상위 현상으로 보이나 흑미의 결정요소인 안토시아닌 C3G의 함유 여부에 관한 유전분석을 시행하면 9:7의 비율로서 전형적인 두 개의 유전자가 모두 관여하는 상보적 유전현상이다. 유전적 정의는 유전자의 최종산물에 의한 물리적 또는 화학적 결정이다. 그러므로 결론하여 검정 쌀의 주요 생리활성물질인 안토시아닌 C3G 생성에 관한 유전현상은 Pb와 Pp 유전자의 상보적 유전자의 상호에 의한 것이다.