• The Journal of Advanced Prosthodontics
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• 제6권4호
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• pp.295-301
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• 2014

PURPOSE. Numerous methods were used to etch the fiber posts to improve its bonding to root canal dentin. Our aim was to evaluate the efficacy of 37% phosphoric acid in etching fiber posts in comparison with 24% hydrogen peroxide. MATERIALS AND METHODS. Ninety human maxillary central incisors were taken and post space preparation was done. Ninety fiber posts were taken and divided into three groups (n=30) based on the surface treatment they received ($H_3PO_4$, $H_2O_2$, distilled water) and each group was further divided (n=10) based on the time period of application (15 seconds, 30 seconds, 60 seconds). All the posts were luted into canals using Rely X UniCem-2. Each tooth was then sectioned into six slices and subjected to push out test. Data obtained was subjected to statistical analysis at P<.05. The surface topography was evaluated using scanning electron microscopy. RESULTS. Highest bond strength values were noted in 15 seconds etched phosphoric acid group and 60 seconds etched hydrogen peroxide group with no significant difference between two groups. Surface topography revealed complete epoxy layer removal with no damage to its structural integrity in those groups. CONCLUSION. $H_3PO_4$ etching for a period of 15 seconds is an effective alternative in improving the adhesion of fiber post to root dentin.

• Clinical and Experimental Pediatrics
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• 제50권8호
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• pp.752-756
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• 2007

목 적 : 만성 기능성 변비 환아의 치료에서 PEG 4000과 락툴로스의 임상적 효용성에 대해 비교 분석 하였다. 방 법 : 2004년 9월부터 2007년 2월까지 노원 을지병원 소아과에 만성 반복성 복통이나 배변 장애를 주소로 내원하여 만성 기능성 변비로 치료받고 1년간 외래 추적이 가능했던 환아 56명을 대상으로 하였다. 전해질이 함유되지 않은 PEG 4000을 투여한 24명의 환아와 락툴로스를 투여한 32명의 환아들을 대상으로 치료 시작시와 치료 1개월, 3개월, 6개월, 1년 후에 각 약물의 효과와 부작용에 대해 비교 분석하였다. 결 과 : 두 군 모두 배변 횟수가 치료전에 비해 유의하게 증가하였고(P<0.05), 복통 또한 치료 전에 비해 유의한 감소가 있었다(P<0.05). 치료를 시작한 시점에서 12개월이 되었을 때 변비와 유분증으로부터의 회복은 PEG 투여군에서는 12례(60%)에서, 락툴로스 투여군에서는 15례(57.7%)에서 있었으며 두 군 간에 유의한 차이는 없었다. 결 론 : 유소아 만성 변비에서 PEG 4000과 락툴로스는 장기적 유지 요법으로 안전하고 효과적인 약물이며 변비와 유분증으로부터의 회복에 두 군간에 유의한 차이는 없었다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2018년도 추계학술대회
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• pp.444-447
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• 2018

Baculovirus 발현 시스템은 박테리아 발현 시스템, 특히 복잡한 번역 후 변형을 필요로 하는 것과 비교하여 다량의 재조합 단백질을 생성하는 빠르고 비용 효율적인 방법을 포함하는 많은 알려진 장점을 갖는다. 특히 재조합 baculovirus는 광범위한 포유류 세포 유형에서 벡터를 전달하고 재조합 단백질을 발현 할 수 있다. 본 연구에서는 pcDNA3.1로부터 재구성된 baculovirus 벡터를 사용하였는데 이 벡터는 cytomegalovirus (CMV) 프로모터, uroplakin II promoter, polyhedron promoter, 수포 구내염 바이러스 G (VSVG), 녹색 형광 단백질 (EGFP), 단백질 전달 도메인 (PTD) 유전자 등 다양한 유전자들로 재조합 되어 개발되었다. 이러한 재구성 된 벡터를 다양한 세포 및 세포주에 감염시켰다. 이렇게 개발된 baculovirus 벡터는 재조합된 유전자들의 전이성 및 발현성을 기존의 일반적인 벡터와 비교하여 분석하였다. 본 연구결과로 이렇게 개발된 baculovirus 벡터는 기존의 대조군 벡터보다 전이성 및 발현성면에서 더 높은 효율을 갖는다는 것을 확인하였다.

• Archives of Plastic Surgery
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• 제34권1호
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• pp.70-76
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• 2007

Purpose: Full-thickness skin grafts are usually used in facial reconstruction, but on occasion, split-thickness skin graft is also used from the scalp due to the limitation of donor site. However, there were complications, such as alopecia, folliculitis and blood loss. In addition, it can not be used in patients with baldness. Under the circumstances, we used medial arm skin as split-thickness skin graft donor site in lieu of scalp. We investigated the efficacy of the medial arm skin as a donor site of facial skin graft in comparison with scalp. Methods: From 2000 to 2005, the split-thicknesss skin grafts were performed using the medial arm skin in 10 patients and the scalp in 10 patients. We inspected the skin color match, texture match by the visual analogue scale. Scar contracture was estimated by the Visitrak $grade^{(R)}$(Smith & Nephew). The statistical analysis was performed by SPSS 12.0. Results: There was a more satisfaction in color match, texture, and scar contracture in medial arm skin than in scalp. Conclusion: According to these results, medial arm skin may be used efficiently as an alternative donor site of scalp in the facial reconstruction.

• 한국응급구조학회지
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• 제26권3호
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• pp.37-46
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• 2022

Purpose: TThe experiment was designed to compare the efficiency of ventilation between conventional BVM ventilation and a newly devised A-BVM ventilation method with Tidal volume, total ventilation rate, average Ventilation speed, and average Ventilation volume. Methods: 40 Paramedical students who agreed to participate in the study were analyzed. Values were measured using IMB PASS after 2 minutes of Brayden Pro manikin with BVM and A-BVM ventilation. The difference in general characteristics was assessed by t-test and ANOVA and the difference in ventilation methods was analyzed by IBM SPSS. Results: A significant difference was found between the two ventilation methods in terms of tidal volume (t=-11.203, p<.001), ventilation time (t=-3.834, p<.001), and optimum ventilation probability (t=10.770, p<.001). A-BVM ventilation method, rather than BVM ventilation method, showed a value close to the appropriate amount recommended by Korean Advanced Life Support (500~600mL) in tidal volume, and higher in optimum ventilation probability. Conclusion: We could identify the a better mode of ventilation. Further studies on the efficacy of existing BVM ventilation methods as compared to device augmented BVM ventilation methods should be carried out to ensure that adequate ventilation is available to patients in clinical practice.

• 융합정보논문지
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• 제12권2호
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• pp.192-197
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• 2022

다양한 연구가 에센셜 오일과 피부 상재균에 관한 초점을 맞추고 있지만, 에센셜 오일의 항균 특성과 미생물에 대한 효능 비교 연구는 거의 없다. 이 연구에서 우리는 옥사실린을 대조군으로 사용하여 표피포도상구균에 대한 10가지 에센셜 오일의 항균 활성을 테스트했다. 두 가지 다른 농도에서 에센셜 오일과 표피포도상구균을 함유한 용액의 활성을 측정하기 위해 광학 밀도(OD)를 사용했다. 옥사실린에 비해 에센셜오일 3종(팔마로사, 레몬그라스, 멜리사 트루)이 항균활성은 더 높았으나 농도는 항균활성에 미미한 영향을 미치는 것으로 나타났다.

• 대한약침학회지
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• 제26권4호
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• pp.319-326
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• 2023

Objectives: The study's goal was to find out whether Chrysanthemum rubellum extract has anti-diabetic properties by concentrating on α-glucosidase and the PTP-1B signaling pathway. C. rubellum flowers were used for extraction using Methanol/water (80/20) as solvent. Methods: LC-MS techniques was used to check the presence of phytoconstituents present in C. rubellum extract. In vitro antidiabetic activity was evaluated using α-glucosidase inhibitory activity and PTP-1B signaling pathway. On Streptozotocin (STZ)-induced rats with diabetes, the in vivo antidiabetic efficacy was assessed using a test for oral glucose tolerance. Results: The phytoconstituents identified in the extract of C. rubellum were apigenin, diosmin, myricetin, luteolin, luteolin-7-glucoside, and Quercitrin as compound 1-6, respectively. Results showed that diosmin exhibited highest α-glucosidase inhibitory activity i.e. 90.39%. The protein level of PTP-1B was lowered and the insulin signalling activity was directly increased by compounds 1-6. The maximum blood glucose levels were seen in all groups' OGTT findings at 30 minutes following glucose delivery, followed by gradual drops. In comparison to the control group, the extract's glucose levels were 141 mg/dL at 30 minutes before falling to 104 mg/dL after 120 minutes. The current study has demonstrated, in summary, that extract with phytoconstituents reduce blood sugar levels in rats. Conclusion: This finding suggests that extract may reduce the chance of insulin resistance and shield against disorders like hyperglycemia.

• 한국식품위생안전성학회지
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• 제34권6호
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• pp.557-561
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• 2019

본 연구에서는 가축분퇴비에 존재할 수 있는 식중독균의 검출을 위하여 기존의 배양을 이용한 방법을 대체할 수 있는 real-time PCR을 적용하고자 하였으며, 이에 따라 유전자 증폭에 영향을 미치는 DNA 추출 방법에 따른 식중독균 검출 효율을 비교하였다. 적용한 방법은 가열 처리, 유기용매 및 흡착제 처리, 효소 처리의 3가지로 구분할 수 있으며, 각 방법에 따른 DNA의 검출 효율을 실험 결과로 나타내었다. 가열 처리 방법에서는 가열 시간의 증가에 따라 DNA 검출 효율이 높아지는 경향을 나타냈으며, 유기용매 및 흡착제는 효과를 나타내지 않았고, 효소 처리의 경우에는 그람 양성균 보다는 그람 음성균의 DNA가 추출 효율이 더 높은 것으로 나타났다. 결론적으로 퇴비에서 30분 이상의 가열 처리와 효소의 처리를 통한 DNA 추출 방법은 real-time PCR을 적용한 식중독균 검출에 적합한 것으로 판단된다.

• 한방재활의학과학회지
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• 제30권3호
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• pp.89-101
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• 2020

Objectives The purpose of this study is to assess the effectiveness of Chuna manual therapy for shoulder pain in hemiplegic patients after stroke. Methods For a systematic review and meta-analysis, we set a key question in accordance with participants, intervention, comparison, outcome, study design (PICO-SD). we searched for clinical studies that conducted chuna manual therapy for shoulder pain in hemiplegic patients after stroke 7 databases until September 2019. Only randomized controlled trials were chosen. Results Total 14 randomized controlled trials were chosen for systematic review and meta-analysis. 9 studies used efficacy rate as evaluation tool, and 8 used visual analogue scale. Pressure method (壓力型) was used in 11 studies, followed by osteopathy method (整骨型) and wave motion method (波動型) in 9 studies. Chuna manual therapy showed statistically significant pain reduction effect. Through meta analysis, Chuna manual therapy showed significant pain reducing effect except the study with Chuna manual therapy and electroacupuncture compared to electroacupuncture only. Conclusion As a result, Chuna manual therapy showed statistically significant effect in pain reduction. However, almost studies were evaluated in a state of having probable high risk of bias. This suggests that it requires attention to make an interpretation in this study. Furthermore, more clinical research need to be accomplished in the future.

• Journal of Preventive Medicine and Public Health
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• 제45권3호
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• pp.156-163
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• 2012

Objectives: It is necessary to examine groups carrying out sexually risky behavior because the prevalence of sexually transmitted diseases (STDs) is high among them. In this study, the prevalence of STDs among homosexuals and sexbuying men in South Korea was investigated, along with their sexual risk factors. Methods: Men who have sex with men (MSMs, n=108) were recruited in Seoul and Busan by applying the time location sampling method, while sex-buying men (n=118) were recruited from a john school in Gyeonggi province, the suburbs of Seoul. Dependent variables included past or present infection with syphilis, Chlamydia, gonorrhea, and human immunodeficiency virus. Independent variables included health behavior, social support, sexual behavior, and safe sex. Results: It was found that when the MSMs were non-drunk while having sexual intercourse (odds ratio [OR], 0.132), they showed a higher STD infection rate when they had a higher number of anal sex partners (OR, 5.872), rarely used condoms (OR, 1.980), had lower self-efficacy (OR, 0.229), and were more anxious about becoming infected with an STD (OR, 3.723). However, the men who paid for sex showed high STD infections when they had more sex partners (OR, 2.286) and lower education levels (OR, 3.028). Conclusions: STD infections among the two groups were high when they were engaged with many sex partners and not having protected sex. In other words, there was a gap in risky sex behavior within such groups, which was significantly related to the possibility of developing an STD. Therefore, the preventive intervention against STDs for these groups needs to be expanded to include management of sex behaviors.