• Journal of Animal Reproduction and Biotechnology
• /
• v.34 no.4
• /
• pp.280-291
• /
• 2019

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.

• Journal of the Korea Fashion and Costume Design Association
• /
• v.7 no.3
• /
• pp.17-26
• /
• 2005

In this research, in order to find out the effect of the area ratio in the harmonic sense assessment of the coloration of Korean clothes, the coloration of a Korean jacket and a skirt was compounded with a similar coloration and a contrast coloration. The respondents were asked to evaluate 16 stimuli of the traditional Korean skirt and jacket with the coloration of the Korean jacket/ skirt, the skirt/ Korean jacket was separately compounded to be 4 tones such as vivid, light, dull, dark. The subjects were 44 female undergraduate students. The results of each can be summerized as follows. As to similar coloration, the korean yellow jacket and red skirt, Korean students feel vivid and dull tone was harmonious, and American students feel vivid, light, and dark tone were harmonious coloration. In inversed case, red korean jacket and yellow skirt, both countries' students recognized that the vivid tone was harmonious. Korean students gave higher points to korean traditional dress in similar coloration such as yellow korean jacket which was small area and red skirt which was large area coloration than inversed color area ratio which red korean jacket and yellow skirt. As to contrast coloration, the korean green jacket and red skirt, Korean students feel vivid tone was harmonious, and American students feel vivid, dark tone were harmonious coloration. In inversed case, red korean jacket and green skirt were recognized that any tone was not harmonious for both countries' students. Both countries of student showed the culture gap. Compared with American students, Korean students were familiar with korean dress's traditional coloration, as a result familiar coloration was recognized harmonious coloration.

• Proceedings of the KOSOMBE Conference
• /
• v.1998 no.11
• /
• pp.281-282
• /
• 1998

Recently, there has been growing interest in the assessment of physiological parameters on brain perfusion that provide more information than pure morphologic diagnosis. Quantification of parameters that characterize cerebral micro-circulation with magnetic resonance imaging is of great relevance for clinical application. We determine the local tissue concentration by exponential relationship between the relative signal reduction S(t)/$S_0$ and local tissue concentration of contrast material $C_m(t)$ in dynamic susceptibility contrast enhanced MR imaging. And then we made relative regional blood volume map by calculating the area under the measured concentration-time curves $C_m(t)$ during first pass of paramagnetic contrast material as a preliminary step for perfusion map. These images make it possible to compare the rCBV in different brain regions in one individual at a time. We have it in contemplation to obtain arterial and brain signal time curves simultaneously to make absolute rCBV and perfusion (rCBF) map. These maps may provide the method of comparative investigations of different patients having strong variation in AIF.

• Korean Journal of Remote Sensing
• /
• v.36 no.1
• /
• pp.67-81
• /
• 2020

The rapid climatic changes being caused by global warming are resulting in abnormal weather conditions worldwide, which in some regions have increased the frequency of landslides. This study was aimed to analyze and compare the landslide susceptibility using the Frequency Ratio (FR), Statistical Index, Weight of Evidence, Certainty Factor, and Index of Entropy (IoE) at Woomyeon Mountain in South Korea. Through the construction of a landslide inventory map, 164 landslide locations in total were found, of which 50 (30%) were reserved to validate the model after 114 (70%) had been chosen at random for model training. The sixteen landslide conditioning factors related to topography, hydrology, pedology, and forestry factors were considered. The results were evaluated and compared using relative operating characteristic curve and the statistical indexes. From the analysis, it was shown that the FR and IoE models were better than the other models. The FR model, with a prediction rate of 0.805, performed slightly better than the IoE model with a prediction rate of 0.798. These models had the same sensitivity values of 0.940. The IoE model gave a specific value of 0.329 and an accuracy value of 0.710, which outperforms the FR model which gave 0.276 and 0.680, respectively, to predict the spatial landslide in the study area. The generated landslide susceptibility maps can be useful for disaster and land use planning.

• Journal of Korean Society of Rural Planning
• /
• v.24 no.2
• /
• pp.47-58
• /
• 2018

Protected horticultural complexes would increase crop productivity but would adversely affect the groundwater recharge function in the area because the impervious area would increase. Further, they would limit the movement of living beings, affecting biodiversity. Therefore, this study evaluated the groundwater ecosystem services provided by protected horticultural complexes in terms of consistent utilization of water. The estimated amounts of groundwater loss obtained through quantitative assessment of groundwater infiltration showed that a higher impervious area results in higher losses. We, therefore, predict a much higher loss if similar changes in land use are realized on a nationwide scale. A plan to promote groundwater recharge in impervious areas is actively being discussed for urban areas; however, this plan is not yet applicable to farming areas. We consider it is essential to develop groundwater infiltration facilities for horticultural complexes, infiltration trenches, permeable pavements, surface water storage facilities, water purification facilities, etc. Further research and development of groundwater infiltration facilities is important for consistent utilization of water and the improvement of ecosystem services.

• Journal of Microbiology
• /
• v.44 no.4
• /
• pp.375-382
• /
• 2006

From its initial colonization to causation of disease, Streptococcus pneumoniae has evolved strategies to cope with a number of stressful in vivo environmental conditions. In order to analyze a global view of this organism's response to heat shock, we established a 2-D electrophoresis proteome map of the S. pneumoniae D39 soluble proteins under in vitro culture conditions and performed the comparative proteome analysis to a 37 to $42^{\circ}C$ temperature up-shift in S. pneumoniae. When the temperature of an exponentially growing S. pneumoniae D39 culture was raised to $42^{\circ}C$, the expression level of 25 proteins showed changes when compared to the control. Among these 25 proteins, 12 were identified by MALDI-TOF and LC-coupled ESI MS/MS. The identified proteins were shown to be involved in the general stress response, energy metabolism, nucleotide biosynthesis pathways, and purine metabolism. These results provide clues for understanding the mechanism of adaptation to heat shock by S. pneumoniae and may facilitate the assessment of a possible role for these proteins in the physiology and pathogenesis of this pathogen.

• Clinics in Shoulder and Elbow
• /
• v.20 no.1
• /
• pp.3-9
• /
• 2017

Background: Many hyaluronic acid (HA)-based anti-adhesive agents have been commercialized for clinical use in the pharmaceutical market. But their efficacy in arthroscopic rotator cuff repairs remains elusive. To determine their efficacy, we performed a comparative analysis of the effects of two hyaluronate/carboxymethylcellulose (CMC)-based anti-adhesive agents, Protescal and Guardix. Methods: We recruited a total of 256 patients who had received an arthroscopic rotator cuff repair at our hospital between January 2014 and March 2015. Among them, 96 patients fulfilled the study's selection criteria and were enrolled as the final population sample. Thirty patients who had received a postoperative injection of Protescal were allocated into Group A. Another 30 patients who had received a postoperative injection of Guardix were allocated into Group B. As controls, 36 patients who did not receive any injection were allocated into Group C. The patients included in this study were aged between 19 and 75 years. For the clinical assessment, we measured the following clinical parameters-the visual analogue scale for pain (PVAS), the American Shoulder and Elbow Surgeons (ASES) score, and the constant score, as well as passive range of motions (ROMs)-at three time-points (preoperatively, 2-month postoperatively, and 6-month postoperatively). Results: We found that Group A compared to Group B tended to show a swifter recovery in passive anterior elevation and in internal rotation by the 2-month postoperative follow-up, but the differences were not statistically significant. Conclusions: We found that the effects of HA/CMC-based injections were minimal after arthroscopic rotator cuff repairs.

• Health Policy and Management
• /
• v.30 no.1
• /
• pp.100-111
• /
• 2020

Background: Few studies have examined the performance of the public long-term care insurance (LTCI) from the perspective of geographic equity. This study investigated regional variations and associated factors in the supply and utilization of nursing home care within and also between Japan and Korea. Methods: A comparative dataset was developed by extracting data from 2013-2015 LTCI statistics yearbooks and Organization for Economic Cooperation and Development regional statistics, as well as other comparable data in Japan and Korea. The unit of analysis was the prefecture in Japan and the province in Korea. We computed variation indices and conducted regression analyses for regional variations within each country and decomposition analyses to examine the variations between the countries. Results: The overall regional supply and use of nursing home care were higher in Japan, but the regional variations in Korea were larger than in Japan. In both countries, the nursing home supply was negatively associated with the proportion of older people with independent living. Nursing home use was also negatively associated with the supply of hospital beds and home care agencies in Korea; the relationship was the opposite in Japan, however. The country-based differences were more likely to be explained by differences in the distributions of the variables included in the analytical model than country-specific characteristics. Conclusion: Regional-level nursing home supply and use were unequal in both countries, and the contributing factors were not the same. Policy efforts are needed to advance regional equality in long-term care (LTC) and collaboration between health and LTC institutions for frail older people, especially in Korea.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.13 no.10
• /
• pp.5179-5196
• /
• 2019

To explore an effective non-invasion medical imaging diagnostics approach for hepatocellular carcinoma (HCC), we propose a method based on adopting the multiple technologies with the multi-parametric data fusion, transfer learning, and multi-scale deep feature extraction. Firstly, to make full use of complementary and enhancing the contribution of different modalities viz. multi-parametric MRI images in the lesion diagnosis, we propose a data-level fusion strategy. Secondly, based on the fusion data as the input, the multi-scale residual neural network with SPP (Spatial Pyramid Pooling) is utilized for the discriminative feature representation learning. Thirdly, to mitigate the impact of the lack of training samples, we do the pre-training of the proposed multi-scale residual neural network model on the natural image dataset and the fine-tuning with the chosen multi-parametric MRI images as complementary data. The comparative experiment results on the dataset from the clinical cases show that our proposed approach by employing the multiple strategies achieves the highest accuracy of 0.847±0.023 in the classification problem on the HCC differentiation. In the problem of discriminating the HCC lesion from the non-tumor area, we achieve a good performance with accuracy, sensitivity, specificity and AUC (area under the ROC curve) being 0.981±0.002, 0.981±0.002, 0.991±0.007 and 0.999±0.0008, respectively.

• Journal of the Korean GEO-environmental Society
• /
• v.7 no.6
• /
• pp.89-100
• /
• 2006

Recently, lots of lives and properties have been lost because comparatively large magnitude earthquakes were occurred in relatively safe regions and nations. It has been reported that number of earthquakes was increased rapidly in Korea. Hence, recently civil constructions were ensured against risks about earthquake not only large-scale structures but also comparative small-scale structures such as reservoir dams and life line by systematic aseismic design. Therefore, in this study, the seismic stability was ensured to evaluate aseismic performance for major planned reservoir dams in Korea. The seismic response analyses were conducted using SHAKE program on new reservoir dams under short-period, long-period and artificial seismic wave. The liquefaction potential for reservoir dams was assessed by using results from seismic response analysis (simplified assessment method for liquefaction potential). Also, fully coupled analysis--interaction of pore-pressure and soil--was performed to investigate both the development of excess pore water pressure and the characteristic of dynamic shear strain.