• Korean Journal of Organic Agriculture
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• v.17 no.1
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• pp.55-62
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• 2009

In this paper, we assessed the growth of rice seedlings(Chucheong variety) in commercial organic growth medium that was substituted with different ratios of vermicast of oyster mushroom waste grown under potting alone, and potting and floor layering treatment. The commercial organic growth medium was substituted with vermicast at ratios of control, 2%, 4% and 6%, respectively. The control consisted of commercial organic growth medium alone without vermicast. Incorporation of $4%{\sim}6%$ vermicast of oyster mushroom waste into a commercial organic growth medium enhanced the growth of rice seedlings significantly as compared to commercial organic growth medium alone(control), and 2% amended with vermicast in potting treatment. This results demonstrate that substitution with low ratios of vermicast($4%{\sim}6%$) will promote growth of rice seedlings. The growth of rice seedlings in commercial organic growth medium alone without vermicast was enhanced significantly as compared to the substituted with vermicast in floor layering treatment, it may due to nutrient uptake by elongated root from the vermicast when applied to on the floor layering. Floor layering treatment is an effective method for potting processing of vermicast. The vermicast of oyster mushroom waste should have a high safety and great potential as materials of growth media for increasing plant growth, either as soil conditioner, or as substitution or amendments to commercial organic growth medium. For the enhanced growth of rice seedlings, demand to increase with total nitrogen, and decrease with the carbon and nitrogen ratio(C/N) of commercial organic growth medium supplied by such as vermicast.

• Korean Journal of Organic Agriculture
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• v.16 no.3
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• pp.299-308
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• 2008

In this study, the effect of commercial organic growth medium amended with different ratios of vermicast on the growth of rice seedlings. The amended ratios of vermicast were 0% (control), 5%, 10%, 20% and 40%, respectively. A bioassay with two rice varieties (Chucheng and Black) was conducted to assesses the amendment effect of vermicast on the seedling growth in two potting methods. In potting treatment, the Chucheng variety with late maturity showed significantly higher values on growth parameters excepted root weight (RW) up to 20% and Black variety with early maturity up to 10% amended with vermicast, respectively. In floor layering treatment, all of the growth parameters significantly increased up to 5% amended with vermicast in both rice variety. The seedling growth of rice in floor layering treatment attained the highest values at lower amended ratios of vermicast than those of potting treated only, it may correspond with increased uptake of nutrient by elongated root grown under the layering amendment of vermicast. Vermicasts can be used as soil amendments or components of plant growth medium for seeding organic agriculture.

• Journal of Mushroom
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• v.2 no.3
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• pp.157-162
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• 2004

The objectives of this experiment were to study the growth and development of fruiting body of the two Ganoderma lucidum isolates on log of the soft wood Paraserianthes falcataria and the hard wood Shorea sp., and determination of organic germanium and crude ganoderic acid content of the fruiting body produced. The two Ganoderma lucidum isolates used were one Indonesian native (Indonesia isolate) and another isolate was purchased from Fungi Perfecti, USA (commercial isolate). The development and quality of the primordium and fruiting body of the mushroom, in general, were influenced by the isolates used. The types of wood, however, had no effect on the quality of the primordium and fruiting body produced. The Indonesian isolate produced better fruiting body compared to that of the commercial isolate. The development of fruiting body from primordium, however, was low for the two isolates tested. In general, only about one third of the primordium developed further into mature fruiting bodies, except for the commercial isolate grown on the soft wood medium in which more than 60% of the primordium developed into mature fruiting body. Apart from producing normal fruiting body, the commercial isolate also produced an abnormal one, which had a white mature pileus, whereas the normal one was brownish red. The organic germanium concentration of the fruiting body produced on the hard wood, in general, was higher than that of grown on the soft wood. The fruiting body from commercial isolate had higher organic germanium concentration compared to that of Indonesian isolate in both wood types. The two isolates used, however, had almost the same value of the crude ganoderic acid concentration in both types of wood tested. The Indonesian isolate had higher total yield of both organic germanium and crude ganoderic acid of the fruiting body produced compared to that of the commercial isolate.

• Korean Journal of Organic Agriculture
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• v.11 no.1
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• pp.55-66
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• 2003

This study was carried out to investigate the effects of different plant growth media on the growth of tomato(Lycopersicum esculentum Mill.) seedlings during growth stages. The media were commercial plant growth medium 100％, earthworm cast (that was produced by vermicomposting of food waste and cattle manure) 100％, earthworm cast 50％ ＋ vermiculite 50％, earthworm cast 50％ ＋ perlite 50％, earthworm cast 40％ ＋ vermiculite 30％ + perlite 30％. Plant length(mm), number of leaves, leaf area($\textrm{cm}^2$), stem diameter(mm), plant dry mater were greatest till the 2nd week growth stages in the commercial plant growth medium plots, but those were higher in the earthworm cast than those in the other plant growth media at the later stages of this study(P<0.05). And relative growth rate of biological yield, relative growth rate of shoot and relative growth rate of root were highest in the earthworm cast till the 4th week growth stage. Therefore it can be implied that there is the possibility of potential utilization of earthworm cast, which was produced by vermicomposting of food waste and cattle manure, as vegetable growth medium.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.443-453
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• 2012

The objectives of this study were to find out proper potting medium and nutrient levels in the soil to promote the root nodule formation in black locust (Robinia pseudoacacia L.) seedlings. Commercial potting medium, compost, organic fertilizer, molded forest fertilizer, and compound fertilizer were used at different mixing rates to bring in various levels of mineral nutrients in the soil. Seedlings were grown in pots in a greenhouse for three months. Commercial potting medium containing peatmoss, vermiculite, and geolite was not suited for early nodule formation due to lack of nutrients, even though it produced good total dry weight. Compost was the best medium to promote both high total dry weight production and nodule formation with providing the proper levels of nitrogen and phosphorus in the soil. Molded forest fertilizer was acceptable for nodule formation. Compound fertilizer and organic fertilizer was not suited for nodule formation. The potting medium should contain optimum levels of nitrogen (0.05-0.2%) and phosphorus (100-600ppm) to promote early nodule formation in black locust seedlings.

• Research in Plant Disease
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• v.22 no.1
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• pp.18-24
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• 2016

This study was conducted to elucidate the cultural and pathogenic characteristics of Cladosporium cucumerinum PT1 and resistance of 81 commercial cucumbers (Cucumis sativus). Cucumber leaves and fruits appeared as scab were collected from a plastic film house located in Pyeongtaek, Gyeonggi-province, Korea in late March, 2015. A casual fungus was isolated from the diseased fruits on potato dextrose agar and it was identified as C. cucumerinum PT1 based on the morphological characteristics. To find out the effect of wounding and fruit size on the development of cucumber scab, small (<10 cm long), medium (10 to 20 cm long), and large (>20 cm long, commercially mature fruit) size cucumber fruits were harvested, C. cucumerinum PT1 pathogens were inoculated with a single droplet of suspension ($1{\times}10^5$ spores/ml) on wounded or unwounded cucumber fruits. Small fruits were completely damaged with showing severe water-soaking symptoms and fast pathogen growth regardless of wounded or unwounded. Meanwhile slight water-soaking symptoms on medium and large size fruits occurred and disease development into plant tissues was observed only on wounded fruits. Disease resistance of 81 commercial cucumber cultivars was evaluated on third-stage seedlings and small fruits by inoculating suspension ($1{\times}10^5$ spores/ml) of C. cucumerinum PT1. As a result, mini and pickling cultivar groups were resistant, 'Cheoeumcheoreom' cultivar was symptomless and the other cultivars were resistant to medium resistant. On the other hand, most of cucumber cultivars belonging to the other groups were susceptible. Disease resistance of cucumber against cucumber scab was significantly different among cultivars and a few cucumber cultivars showed different disease resistant responses to two bioassay methods using seedlings and small fruits. Therefore, to screen scab resistance in cucumber, a test using both fruits and seedlings is advisable. We think that the selected resistant cultivars can be used to control cucumber scab effectively under the farmhouse condition.

• Mycobiology
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• v.39 no.2
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• pp.85-91
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• 2011

We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of $25^{\circ}C$ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, $K_3PO_4$ showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and $K_3PO_4$ (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.

• Microbiology and Biotechnology Letters
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• v.48 no.3
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• pp.358-365
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• 2020

An organic solvent- and bleach-stable protease-producing strain was isolated from a polluted river water sample and identified as Aeromonas veronii OB3 on the basis of biochemical properties (API 20E) and 16S rRNA sequence analysis. The strain was found to hyper-produce alkaline protease when cultivated on fish waste powder-based medium (HVSP, 4080 U/ml). The biochemical properties and compatibility of OB3 with several detergents and additives were studied. Maximum activity was observed at pH 9.0 and 60℃. The crude protease displayed outstanding stability to the investigated surfactants and oxidants, such as Tween 80, Triton X-100, and H₂O₂, and almost 36% residual activity when incubated with 1% SDS. Remarkably, the enzyme demonstrated considerable compatibility with commercial detergents, retaining more than 100% of its activity with Ariel and Tide (1 h, 40℃). Moreover, washing performance of Tide significantly improved by the supplementation of small amounts of OB3 crude protease. These properties suggest the potential use of this alkaline protease as a bio-additive in the detergent industry and other biotechnological processes such as peptide synthesis.

• Korean Chemical Engineering Research
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• v.53 no.1
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• pp.78-82
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• 2015

Hollow silica particles were prepared using sodium silicate and organic templates. Polystyrene latex (PSL) particles produced by dispersion polymerization were used as organic templates. PSL particles ranged from $1{\mu}m$ to $3{\mu}m$ in diameter were synthesized by adjusting the amount of 2,2'-azobisisobutyronitrile (AIBN). The PSL/$SiO_2$ core-shell particles were prepared by coating of silica nanoparticles originated from sodium silicate using sol-gel method. The organic templates were removed by the organic solvent, tetrahydrofuran (THF). Morphology of hollow silica particles was investigated with respect to types of the reaction medium and pH during the process. By changing the solvent from ethanol to water, hollow silica particles were successfully formed. Hollow silica particles with the uniform shell thickness were produced at low pH as well. The reflectivity of the as-prepared silica particles was measured in the range of the wavelength of UV and visible light. Hollow silica particles showed much better reflective properties than the commercial light reflector, Insuladd.

• Korean Chemical Engineering Research
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• v.53 no.2
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• pp.211-215
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• 2015

The hydrolysis of defatted rice bran using near-critical water was performed, and the feasibility of consequent hydrolyzate as a growth medium was investigated by the cultivation of Saccharomyces cerevisiae. The near-critical water hydrolysis was carried out through a series of batch experiments, and the contents of total carbohydrates, disaccharides, and monosaccharides, total organic carbon (TOC), total nitrogen (TN), pH of products were measured. The growth rate of Saccharomyces cerevisiae was measured with optical density. The yield of total carbohydrates, TOC, and TN increased with temperature below $240^{\circ}C$, however, decreased above $240^{\circ}C$. The decrease of yields above $240^{\circ}C$ was caused by the formation of organic acids, and it agreed with the change of pH of products. The yield of glucose was a maximum at $200^{\circ}C$ and it decreased dramatically at higher temperature. The growth rate of Saccharomyces cerevisiae cultivated in the hydrolyzate was similar with that in the commercial medium under certain conditions. The growth rate was correlated with the content of glucose in hydrolyzate.