• Journal of Microbiology and Biotechnology
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• v.21 no.12
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• pp.1299-1305
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• 2011

An important modification of thrombolytic agents is resistance to plasminogen activator inhibitor-1 (PAI-1). In previous studies, a new truncated PAI-1-resistant variant was developed based on deletion of the first three domains in t-PA and the substitution of KHRR 128-131 amino acids with AAAA in the truncated t-PA. The novel variant expressed in a static culture system of Chinese Hamster Ovary (CHO) DG44 cells exhibited a higher resistance to PAI-1 when compared with the full-length commercial drug; Actylase. In the present study, the truncated-mutant protein was expressed in CHO DG44 cells in 50 ml orbital shaking bioreactors. The final yield of the truncated-mutant in the culture was 752 IU/ml, representing a 63% increase compared with the static culture system. Therefore, these results suggest that using the combined features of a transient and stable expression system is feasible for the production of novel recombinant proteins in the quantities needed for preclinical studies.

• International Journal of Aeronautical and Space Sciences
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• v.18 no.2
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• pp.279-289
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• 2017

In this study, a series of conjugate heat transfer (CHT) analyses are conducted for a stage of a fully cooled high-pressure turbine (HPT) at elevated levels of free stream turbulence (Tu = 5% and 25.7%). The goal of the analyses is to investigate the influence of high turbulence intensity on the fluid-thermal characteristics of a nozzle guide vane (NGV). The turbine inlet temperature is defined by considering a typical radial temperature distribution factor (RTDF). The Unsteady Reynolds Average Navier-Stokes (URANS) CHT simulations are carried out using CFX 15.0, a commercial CFD package. The presented CFD modeling approach for high turbulence intensity is verified with the experimental data from two types of NASA C3X NGVs with films. The computation grid is generated for both the fluid and solid domains. The fluid domain grid is created using a tetrahedral grid system with prism layers because of its complex geometry, and the solid domain grid is composed of only tetrahedral elements. The analytical results are compared to understand the effect of turbulence on flow characteristics and metal temperature distributions. The results obtained in this study provide useful insights on the effects of high free stream turbulence and unsteadiness. The results also lead to the proposal of meaningful turbine design guidelines.

• Spatial Information Research
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• v.20 no.6
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• pp.99-107
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• 2012

Recently, various researches have been attempted to connect or integrate BIM handling construction data and GIS handling spatial data. However, it is hard to interoperate by integration between two data models because characteristics and domains of data models between BIM and GIS are very different. In this research, we propose the platform to secure efficient interoperability between BIM and GIS and define the component technologies and functions of the platform proposed in this research by analyzing the existing commercial softwares.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2008.04a
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• pp.980-985
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• 2008

This paper presents vehicle road test of a semi-active suspension system equipped with continuously controllable magnetorheological (MR) dampers. As a first step, front and rear MR dampers are designed and manufactured based on the optimized damping force levels and mechanical dimensions required for a commercial middle-sized passenger vehicle. After experimentally evaluating dynamic characteristics of the MR dampers, the test vehicle is prepared for road test by integrating current suppliers, real-time data acquisition system and numerous sensors such as accelerometer and gyroscope. Subsequently, the manufactured four MR dampers (two for front parts and two for rear parts) are incorporated with the test vehicle and a skyhook control algorithm is formulated and realized in the data acquisition system. In order to emphasize practical aspect of the proposed MR suspension system, road tests are undertaken on proving grounds: bump and paved roads. The control responses are evaluated in both time and frequency domains by activating the MR dampers.

• Journal of computational fluids engineering
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• v.5 no.1
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• pp.1-7
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• 2000

In general, FDM(finite difference method) and FVM(finite volume method) are used for analyzing the fluid flow numerically. However it is difficult to apply them to problems involving complex geometries, multi-connected domains, and complex boundary conditions. On the contrary, FEM(finite element method) with coordinates transformation for the unstructured grid is effective for the complex geometries. Most of previous studies have used commercial codes such as KIVA or STAR-CD for the flow analyses in the engine cylinder, and these codes are mostly based on the FVM. In the present study, using the FEM for three-dimensional, unsteady, and incompressible Navier-Stokes equation, the velocity and pressure fields in the engine cylinder have been numerically analyzed. As a numerical algorithm, 4-step time-splitting method is used and ALE(arbitrary Lagrangian Eulerian) method is adopted for moving grids. In the Piston-Cylinder, the calculated results show good agreement in comparison with those by the FVM and the experimental results by the LDA.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.19 no.3
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• pp.235-242
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• 2009

This paper presents vehicle road test of a semi-active suspension system equipped with continuously controllable magnetorheological(MR) dampers. As a first step, front and rear MR dampers are designed and manufactured based on the optimized damping force levels and mechanical dimensions required for a commercial middle-sized passenger vehicle. After experimentally evaluating dynamic characteristics of the MR dampers, the test vehicle is prepared for road test by integrating current suppliers, real-time data acquisition system and numerous sensors such as accelerometer and gyroscope. Subsequently, the manufactured four MR dampers(two for front parts and two for rear parts) are incorporated with the test vehicle and a skyhook control algorithm is formulated and realized in the data acquisition system. In order to emphasize practical aspect of the proposed MR suspension system, road tests are undertaken on proving grounds: bump and paved roads. The control responses are evaluated in both time and frequency domains by activating the MR dampers.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.5
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• pp.463-471
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• 2013

This paper presents design and control of electronic control suspension(ECS) equipped with controllable magnetorheological(MR) damper for passenger vehicle. In order to achieve this goal, a cylindrical type MR fluid damper that satisfies design specification of a middle-sized commercial passenger vehicle is proposed. After manufacturing the MR damper with design parameters, their field-dependent damping forces are experimentally evaluated and compared with those of a conventional damper. A quarter-vehicle MR ECS system consisting of sprung mass, spring, tire, controller and the MR damper is established in order to investigate the ride comfort performances. On the basis of the governing equation of motion of the suspension system, five control strategies(soft, hard, comfort, sport and optimal mode) are formulated. The proposed control strategies are then experimentally realized with the quarter-vehicle MR ECS system. Control performances such as vertical acceleration of the car body and tire deflection are evaluated in frequency domains on random road condition. In addition, performance comparison of WRMS(weighted root mean square) of the quarter-vehicle MR ECS system on random road are undertaken in order to investigate ride comfort characteristics.

• Fire Science and Engineering
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• v.21 no.3
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• pp.104-112
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• 2007

Simulators were introduced in education as a tool to make advanced training standardized, less expensive, and without danger to those involved. In 1922 in the Unites States, Edward Link presented his homemade flight simulator, which became common place in both military and civilian aviation, known as the "Link Trainer". The development of mannequin simulators used for medical simulation education, training, and research is reviewed, tracing the motivations, evolution to commercial availability, and efforts toward assessment of efficacy of those for teaching cardiopulmonary resuscitation(CPR) for medical personnel and emergency medical technicians(EMT), cardiology skills, anaesthesia clinical skills, and crisis management. This study will provide a brief overview of simulators and trainers in several domains.

• Journal of KIISE:Computing Practices and Letters
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• v.10 no.2
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• pp.125-133
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• 2004

In order to test reflecting the features of a development methodology and domain, it is required to tailor process standards and perform testing according to the tailored process. However, since commercial testing tools support only a part of the tailored process, it is essential to either acquire or develop testing tools appropriate for a development environment. This paper proposes a method to develop a test process execution tool which has common features of standards, and variousness in methodologies and domains. ‘Test Process Execution Tool: Test PET’ which is a test process execution tool developed adapting the concept of product line. Our Test PET generates the test process suitable for the development methodology and domain and then executes the produced test process.

• Journal of Information Technology Services
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• v.8 no.1
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• pp.203-215
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• 2009

This paper illustrates the application of co-occurrence theory to generate lightweight ontologies semi-automatically. The proposed model includes three steps of a (Semi-) Automatic creation of Ontology; (they are conceptually named as) the Syntactic-based Ontology, the Semantic-based Ontology and the Ontology Refinement. Each of these three steps are designed to interactively work together, so as to generate Lightweight Ontologies. The Syntactic-based Ontology step includes generating Association words using co-occurrence in web documents. The Semantic-based Ontology step includes the Alignment large Association words with small Ontology, through the process of semantic relations by contextual terms. Finally, the Ontology Refinement step includes the domain expert to refine the lightweight Ontologies. We also conducted a case study to generate lightweight ontologies in specific domains(news domain). In this paper, we found two directions including (1) employment co-occurrence theory to generate Syntactic-based Ontology automatically and (2) Alignment large Association words with small Ontology to generate lightweight ontologies semi-automatically. So far as the design and the generation of big Ontology is concerned, the proposed research will offer useful implications to the researchers and practitioners so as to improve the research level to the commercial use.