• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1210-1214
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• 2010

Many bacteria are involved in the fermentation of doenjang, and Bacillus species are known to perform significant roles. Although SDS-PAGE has been frequently used to classify and identify bacteria in various samples, the microbial diversity in doenjang has not yet been investigated. This study aims to determine the identity and distribution of dominant Bacillus species in doenjang using SDS-PAGE profiles of whole-cell proteins and 16S rRNA gene sequencing. Reference Bacillus strains yielded differential SDS-PAGE banding patterns that could be considered to be highly specific fingerprints. Grouping of bacterial strains isolated from doenjang samples by whole-cell protein patterns was confirmed by analysis of their 16S rRNA gene sequences. B. subtilis was found to be the most dominant strain in most of the samples, whereas B. licheniformis and B. amyloliquefaciens were less frequently found but were also detected in several samples. The results obtained in this study show that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully identify Bacillus species isolated from doenjang.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.22
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• pp.9797-9800
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• 2014

Background: Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and Southeast Asia. Alu elements are among the most prevalent repetitive sequences and constitute 11% of the human genome. Although Alu methylation has been evaluated in many types of cancer, few studies have examined the levels of this modification in serum from NPC patients. Objective: To compare the Alu methylation levels and patterns between serum from NPC patients and normal controls. Materials and Methods: Sera from 50 NPC patients and 140 controls were examined. Quantitative combined bisulfite restriction analysis-Alu (qCOBRA-Alu) was applied to measure Alu methylation levels and characterize Alu methylation patterns. Amplified products were classified into four patterns according to the methylation status of 2 CpG sites: hypermethylated (methylation at both loci), partially methylated (methylation of either of the two loci), and hypomethylated (unmethylated at both loci). Results: A comparison of normal control sera with NPC sera revealed that the latter presented a significantly lower methylation level (p=0.0002) and a significantly higher percentage of hypomethylated loci (p=0.0002). The sensitivity of the higher percentage of Alu hypomethyted loci for distinguishing NPC patients from normal controls was 96%. Conclusions: Alu elements in the circulating DNA of NPC patients are hypomethylated. Moreover, Alu hypomethylated loci may represent a potential biomarker for NPC screening.

• KIISE Transactions on Computing Practices
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• v.22 no.11
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• pp.583-590
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• 2016

Recently, the rapidly increasing interest in IoT in almost all areas of casual human life has led to wide acceptance of speech recognition as a means of HCI. Simultaneously, the demand for speech recognition systems for mobile environments is increasing rapidly. The server-based speech recognition systems are typically fast and show high recognition rates; however, an internet connection is necessary, and complicated server computation is required since a voice is recognized by units of words that are stored in server databases. In this paper, we present a novel method for recognizing the Korean vowel 'ㅗ', as a part of a phoneme based Korean speech recognition system. The proposed method involves analyses of waveform patterns in the time domain instead of the frequency domain, with consequent reduction in computational cost. Elementary algorithms for detecting typical waveform patterns of 'ㅗ' are presented and combined to make final decisions. The experimental results show that the proposed method can achieve 89.9% recognition accuracy.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.40 no.4
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• pp.227-236
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• 2003

This paper proposes a modified jointly-blue noise mask (MJBNM) method using the S-CIELAB color measure as digital color halftoning method. Based on an investigation of the relation between the pattern visibility and the chromatic error, of a blue noise pattern, a halftoning method is proposed that reduces the chromatic error, while preserving a high quality blue noise pattern. Accordingly, to reduce the chrominance error, the low-pass filtered error and S-CIELAB chrominance error are both considered during the mask generation procedure and calculated for single and combined patterns. Using the calculated low-pass filtered error, the patterns are then updated by either adding or removing dots from the multiple binary patterns. Finally, the pattern exhibiting the lower S-CIELAB chrominance error is selected. Experimental results demonstrated that the proposed algorithm can produce a visually pleasing half toned image with a lower chrominance error than the JBNM method.

• Journal of Ecology and Environment
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• v.44 no.2
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• pp.62-71
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• 2020

Understanding vegetation structure and the relationship with environmental factors has been crucial for restoration and conservation of riparian zones. In this study, we conducted field survey in a riparian zone of Namhan River in South Korea both before and after flooding in order to understand temporal and spatial variations of riparian vegetation. There were significant temporal and spatial variations in species composition, and distribution patterns of vegetation were different along a gradient of elevation above the water level. At low elevation, Zizania latifolia was dominant throughout the field survey periods, and Bidens frondosa began to grow late and dominated both in post-flooding 1 and 2. Prior to flooding, Scirpus radicans and Polygonum thunbergii were widely distributed at middle elevation, while Artemisia vulgaris, Phragmites australis, and Miscanthus sacchariflorus were dominant at high elevation. After flooding, P. thunbergii was dominant at middle elevation with most other species decreasing, and more invasive or pioneer plants, including Artemisia princeps, H. scandens, and Sicyos angulatus, were observed at high elevation. Species composition and distribution patterns were homogeneous at low elevation, whereas dynamic variations of vegetation were observed both temporally and spatially at higher elevations. Elevation and distance from the water front were the most principal factors governing vegetation structure. Furthermore, soil physicochemical properties were also found to determine species composition and distribution patterns. These results indicate that vegetation structure in the riparian zones is formed by the combined effects of hydrological regime and soil physicochemical properties, inherent characteristics of species, and interspecific competition. Understanding of temporal and spatial variations of riparian vegetation may provide useful insights into ecological restoration and conservation of the vegetation within the riparian zones.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6069-6075
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• 2013

Background: At present, the diagnosis of colorectal cancer (CRC) requires a colorectal biopsy which is an invasive procedure. We undertook this pilot study to develop an alternative method and potential new biomarkers for diagnosis, and validated a set of well-integrated tools called ClinProt to investigate the serum peptidome in CRC patients. Methods: Fasting blood samples from 67 patients diagnosed with CRC by histological diagnosis, 55 patients diagnosed with colorectal adenoma by biopsy, and 65 healthy volunteers were collected. Division was into a model construction group and an external validation group randomly. The present work focused on serum proteomic analysis of model construction group by ClinProt Kit combined with mass spectrometry. This approach allowed construction of a peptide pattern able to differentiate the studied populations. An external validation group was used to verify the diagnostic capability of the peptidome pattern blindly. An immunoassay method was used to determine serum CEA of CRC and controls. Results: The results showed 59 differential peptide peaks in CRC, colorectal adenoma and health volunteers. A genetic algorithm was used to set up the classification models. Four of the identified peaks at m/z 797, 810, 4078 and 5343 were used to construct peptidome patterns, achieving an accuracy of 100% (> CEA, P<0.05). Furthermore, the peptidome patterns could differentiate the validation group with high accuracy close to 100%. Conclusions: Our results showed that proteomic analysis of serum with MALDI-TOF MS is a fast and reproducible approach, which may provide a novel approach to screening for CRC.

• Journal of the Korean Society of Costume
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• v.62 no.1
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• pp.90-103
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• 2012

Felt, is one of the Korean traditional woolen fabrics, that is thermally excellent and durable, so it has been widely used for various living goods from the ancient time to the modern society. Felt makes various changes in colors and shapes, does not change easily even after a long-term use, and is easy to clean. In this study, the origin and history of felt were researched. Also, different kinds and shapes of felt and the currently used manufacturing technique, and how the Korean traditional pattern-applied cultural products were developed using felt were examined as well. The method of this study were as follows. First, felt manufacturing techniques used after investigating the precedent studies, books, records and literatures of museums, the existing relics of felt were examined. Second, the conditions of the traditional pattern-applied cultural products were found out through the precedent studies after examination of kinds and symbols of the Korean traditional patterns. Third, patterns that were applied to cultural products were selected and designed with new patterns. Fourth, the past techniques were conducted with the following three methods by referring to the range of cultural products manufacturing; )color felt made with one color, pattern felt, and pattern-embroidered felt. Fifth, the following products combined with decoration and practical application by applying felt techniques were used. This study was aiming at the development of practical cultural products made with the traditional wool, felt, which can receive a good response from all age groups. It is allegedly required to develop various cultural products through studies and the rediscovery of the national traditional materials. In the future, it is necessary to study in such a way that diversification and differentiation are made through approaches and attempts with various techniques.

• Journal of muscle and joint health
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• v.8 no.2
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• pp.250-261
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• 2001

Alternative therapy for rheumatoid arthritis has become worldwide phenomenon this decade without scientific validation. This study was undertaken to explore the prevalence, patterns of use, and perceived efficacy of alternative therapies by patients attending a rheumatology clinic. We conducted a crossectional survey of 142 RA patients with structured questionnaire at outpatient rheumatic clinic from July to August in 2001. Results of this study were summarized as follows : 1. 85.9% of subjects were woman. Mean age was 49.97years, duration of rheumatoid arthritis was 5.6years. Mean pain score was 5.29cm. 2. 74.6% of subjects perceived rheumatoid arthritis as a controllable disease. 31% of patients combined medical treatments with alternative therapies. 58.5% of patients perceived that combining medical treatments with alternative therapies is the most effective strategy in controlling disease. 3. 77.5% had used more than one alternative therapy since the diagnosis was made. The major reason of discontinuation of use was no effect for the treatment of their disease. 4. 34.5% were currently using an alternative therapies and 50.9% were going to use alternative therapies in future. 5. 42.7% reported that alternative therapies was effective and only 3.6% reported side effects. 6. The perceived effect score was 3.14cm in general expecially the physiological score 2.91cm, psychological score 4.16cm. In conclusion, our results demonstrate a high use of alternative therapies by rheumatoid arthritis patients. And the perceived effect score of physiological aspect was not higher than one of psychological aspect. Therefore it should be considered in education program for the use of alternative therapies.

• Fashion & Textile Research Journal
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• v.10 no.6
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• pp.989-996
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• 2008

The purpose of this study is to provide the basic knowledge for the pattern development of a flared skirt. Two flat pattern (FP) methods, the concentric circle method and the corrected concentric circle method, and a draping were used for pattern making. Using these patterns, skirts were made with lengthwise grain in the centerline. The influence of pattern making method on the silhouette of the flared skirt was evaluated by the hemlines formed at the skirt worn in a dress form. Moreover, the combined influence of pattern making method and cutting direction on the silhouette of the flared skirt was examined with the draping pattern (DP) skirt and a FP skirt with $45^{\circ}$ bias grain in the centerline. The DP skirt had more uniform nodes and formed a stable wave form than the FP skirts throughout the whole hemlines. The number of nodes was reduced with lining in the two FP skirts, whereas the number of nodes was not changed in the DP skirt. The unit wave form of all the skirts by three patterns showed long loop form, and the slope angles of the unit wave form of the two FP skirts were higher than that of the DP skirt. The silhouette of the flared skirt was highly influenced by the shape of the waist circumference line in patterns. The DP skirt with lengthwise grain in the centerline showed good silhouette with uniform nodes and high marking efficiency, compared to the FP skirt with $45^{\circ}$ bias grain in the centerline.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.10B
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• pp.1076-1085
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• 2009

In this paper, a new method for efficient computation of CGH patterns for 3-D video images is proposed by combined use of temporal redundancy and look-up table techniques. In the conventional N-LT method, fringe patterns for other object points on that image plane can be obtained by simply shifting these pre-calculated PFP (Principle Fringe Patterns). But there have been many practical limitations in real-time generation of 3-D video holograms because the computation time required for the generation of 3-D video holograms must be massively increased compared to that of the static holograms. On the other hand, as ordinary 3-D moving pictures have numerous similarities between video frames, called by temporal redundancy, and this redundancy is used to compress the video image. Therefore, in this paper, we proposed the efficient hologram generation method using the temporal redundancy of 3-D video image and N-LT method. To confirm the feasibility of the proposed method, some experiments with test 3-D videos are carried out, and the results are comparatively discussed with the conventional methods in terms of the number of object points and computation time.