• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.116-116
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• 2002

Further development of reconstructed embryos may be dependent upon the synchronization of donor nucleus and recipient cytoplasm at cell fusion, To control the synchronization of donor and recipient cells, the enucleated MII arrested oocytes are artificially stimulated prior to embryo reconstruction. Destruction of cyclin B results in the exit of cells from M-phase of cell cycle. This study was designed to investigate the effects of single or combined stimulation affected cyclin B1 mRNA and protein levels in mouse oocytes. The oocyte activation was induced by 7% ethanol or 10$\mu\textrm{g}$/$m\ell$ Ca-ionophore without (single) or with (combined) 10$\mu\textrm{g}$/$m\ell$ cycloheximide. Competitive quantitative PCR for cyclin Bl mRNA and western blot analysis for cyclin B1 protein was preformed in mouse oocytes. Cyclin B1 mRNA level was significantly reduced in single (P<0.05) and combined (P<0.05) stimulation groups. However, this level did not change in non-activated group and increased in intact group. Cyclin B1 protein level was also significantly reduced in both single (P<0.05) and combined (P<0.05) stimulation groups. In conclusion, single and combined stimulation induces the degradation of cyclin B1 mRNA and protein after activation in enucleated mouse oocytes.

• Journal of The Korean Society of Integrative Medicine
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• v.7 no.4
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• pp.273-282
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• 2019

Purpose : The purpose of this study was to provide the effective method for exercise therapy in soccer player. Methods : 30 soccer players were recruited for subjects. The subjects were divided to dynamic stretching exercise combined tDCS (Group I), dynamic stretching exercise combined Sham-tDCS (Group II) of which 15 subjects were randomly allocated. The subjects were given 3 hour of soccer skill and performance and additionally given 20 minutes of dynamic stretching exercise combined tDCS and sham-tDCS for each group, five times a week, for 6 weeks. Their muscle activity and jump performance were analyzed before the intervention. After 6 weeks of the intervention, the mentioned parameters were measured once more for between-group analysis. Results : Comparative analysis of the muscle activity and jump performance between the groups I and groups II showed statistically significant difference. Conclusion : Such results revealed that dynamic stretching exercise combined with tDCS is effective in muscle activity and jump performance. Based on the current study, more effective program is to be proposed for neurologic and musculoskelectal disorder as well as soccer player. Based on the current study, studies that incorporates various combine of variable is required for development of effective tDCS program.

• PNF and Movement
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• v.18 no.2
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• pp.195-204
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• 2020

Purpose: The study investigated the effect of combined balance exercise using visual feedback and balance pads in rehabilitation of chronic stroke patients. Methods: The participants were 30 patients diagnosed with stroke who met the study selection criteria. Participants were randomly divided into 3 groups of 10: a balance pad exercise (BPE) group, a visual feedback exercise (VFE) group, and a combined balance exercise (CBE) group. All three groups engaged in 30 minutes of exercise, 3 times per week, for 6 weeks. Results: Pre-test and post-test results were analyzed using the functional reach test (FRT), the Berg balance scale (BBS), the timed up & go test (TUG), and the Korean version of the activities-specific balance confidence (K-ABC) scale. The study yielded the following results. Pre- and post-program FRT measures showed significant differences between the BPE and CBE groups (p < 0.05). BBS, TUG, and K-ABC scores showed clear differences in all three groups. Secondly, the CBE group and the BPE group a differed significantly on the BBS before and after the 6-week program (p < 0.05). Finally, the CBE group and the VFE group differed significantly on the BBS before and after the 6-week program (p < 0.05). Conclusion: The study results indicate that combined balance exercise improves balance more effectively than the balance pad and visual feedback exercises. This finding should help to improve rehabilitation in the future.

• Journal of Korean Society of Rural Planning
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• v.23 no.4
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• pp.111-126
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• 2017

This study analyzes the impacts of agricultural water shortages in Korea using a combined top-down and bottom-up model. A multi-region multi-output agricultural sector model with detailed descriptions of production technologies and water and land resource constraints has been combined with a standard CGE model. The impacts of four different water shortage scenarios were simulated. It is shown that an active adaptation of crop choices occurs in even the regions with relatively abundant water resources in order to respond to the change in relative output prices caused by water shortages. We found that although the losses in production values are not quite large despite water shortages due to the price feedbacks, the loss in GDP is substantial. We show that our combined approach has advantages in deriving region and product specific production effects as well as the overall GDP loss effect of water shortages.

• Restorative Dentistry and Endodontics
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• v.23 no.1
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• pp.296-303
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• 1998

The purpose of this study was to assess the effects of cavity designs on the marginal leakage of class 5 glass ionomer restorations. The five cavity designs were as follows ; notch shape(A group), notch shape with groove(B group), combined lesion(C group), combined lesion with groove and deep chamfer margin(D group) and combined lesion with groove and shoulder(E group), and each design had 10 cavities. After the cavities were restored with GIC, they were immersed in 0.5% basic fuchsin solution for 6 hours. The specimens were washed thoroughly and sectioned longitudinally in a buccolingual direction through the center of the restorations. The degree of marginal leakage was measured as the extent of dye penetration under the stereoscope. The results of this study were as follows 1. The enamel margins of all groups showed lesser leakage than dentin/cementum margins(p<0.05). 2. The combined lesion(C group) showed more leakage than notch shape(A group), but there was no siginificant difference(p>0.05). 3. In the notch shape, there was no influence on the marginal leakage by the groove preparation. 4. In the combined lesion, marginal leakage was decreased by the groove preparation and marginal modification.

• 한국노년학
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• v.28 no.4
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• pp.941-952
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• 2008

The purpose of this study was to improve the physical strength through the application of the recreation combined exercise program for elderly women. The subjects of this study were consisted of 20 elderly women were 75 year ~ 88 year. The recreation combined exercise program was executed 50 minute a day, 3times a week for 8 weeks. The result was significantly improved in terms of the grip strength, sit to stand, timed up & go, one leg with eyes opened, sit and reach, 4.6meter fast walking speed. Therefore, recreation combined exercise program expect that it was helpful for the elderly women in terms of physical strength.

• PNF and Movement
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• v.21 no.1
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• pp.11-18
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• 2023

Purpose: This study aims to compare the effects of obstacle walking training combined with cognitive tasks on balance, gait, and activities of daily living in patients with stroke. Methods: A single-subject design was used, where one stroke patient participated. Obstacle walking training combined with cognitive tasks was performed for 1 hour per day for a total of 10 times during the intervention phase. The subjects were measured five times in the baseline phase, 10 times in the intervention phase, and five times in the follow-up phase. The outcome measurements included the Berg balance scale (BBS), the 10-meter walk test (10 MWT), and the Korean modified Barthel index (K-MBI). Results: In this study, the results showed that the 10 MWT scores during the intervention period improved and that this improvement remained, even during the post-period. In addition, BBS and K-MBI values for stroke patients increased significantly after training. Conclusion: The results of this study revealed that obstacle walking training combined with cognitive task training may be helpful to improve balance, gait, and activities of daily living in stroke patients. Therefore, obstacle walking training combined with cognitive tasks is recommended for stroke patients.

• Natural Product Sciences
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• v.13 no.3
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• pp.258-262
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• 2007

The antifungal activity of the essential oil fraction from Thymus magus, and its major component thymol, against Candida albicans was investigated in vitro and in vivo. The combined effects of the oils and clotrimazole, a commonly used antifungal drug for treatment of external candidiasis, were evaluated in this study. In experimental vaginal candidiasis the essential oil fraction of T. magnus resulted in relatively milder inhibition of fungal growth following the inoculation of test mice compared to clotrimazole. However, new fungal growth was not detected up to 12 days after cessation of treatment. In contrast, in a similar experiment using clotrimazole, C. albicans was detected in the $12^{th}$ day post-treatment with the sample. This result indicates that T. magnus oil could be a promising drug to control vaginal candidiasis. In checkerboard titer tests, the combination of clotrimazole with the essential oil fraction of T. magus or T. quinquecostatus resulted in significant synergism, with FIC indices between 0.14 and 0.27 against C. albicans, while clotrimazole combined with thymol, the major component of these oils, produced only an additive effect, with FIC indices ranging between 0.50 and 1.00. Thus, the prominent synergistic effects of clotrimazole combined with T. magus essential oil indicate that these compounds may be an effective treatment for C. albicans infections.

• Journal of Acupuncture Research
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• v.25 no.3
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• pp.115-125
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• 2008

Diabetes mellitus is a worldwide epidemic. Global projections suggest that most nations will have a doubling of the incidence of Diabetes mellitus(DM) within 20years. The Herb-Combined Remedy(HCR) of DM was known as one of anti-hyperglycaemic agents. But it is unclear whether HCR can be used to treat patients with Diabetic nephropathy or not. The present study was designed to investigate the anti-hyperglycaemic and preventive effects from diabetic nephropathy of HCR in STZ-induced diabetic rats. Treatment with HCR regulated blood glucose levels, but did not affect body weights. In addition, HCR was able to decrease blood glucose levels after 120min. Serum BUN levels were not effective but, serum Creatinine levels were lowered by treatment with HCR or MF. In histopathological observation of renal tissue, both HCR and MF groups showed decreased tendency of inflammation and renal injury. In conclusion, these results demonstrate that HCR is effective to treat patients with DM and also has preventive activity on diabetic nephropathy.

• Korean Journal of Plant Resources
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• v.26 no.6
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• pp.695-700
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• 2013

Flax (Linum usitatissimum L.) has been used for the only edible oil in Korea. We carried out the field experiment in order to investigate the possibly combined effects of mepiquat chloride (MC) and trinexapac-ethyl (TE) on oil composition, lignan content, seed yield and endogenous gibberellins content of flax cultivar. Plant growth retardants mepiquat chloride (300 and 600 ppm) and trinexapac-ethyl (100, 200 and 300 ppm) were foliar-sprayed to flax plant at 50days after seeding. The plant height was decreased in the combination of mepiquat chloride 600 ppm with trinexapac-ethyl 100, 200 and 300 ppm. Mepiquat chloride treatment combined with trinexapac-ethyl observed the highest response on seed yield, followed by mepiquat chloride 300 ppm with trinexapac-ethyl 100 ppm, mepiquat chloride 300 ppm with trinexapac-ethyl 200 ppm and mepiquat chloride 300 ppm with trinexapac-ethyl 300 ppm. Lignan content was increased in all of the combination treatments. It concludes that the combination of mepiquat chloride 300 ppm with trinexapac-ethyl 300 ppm will be useful to increasing oil and lignan content in flax plants.