• Asian Pacific Journal of Cancer Prevention
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• v.16 no.16
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• pp.6991-6996
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• 2015

In the past decade, the incidence and mortality rates of cholangiocarcinoma (CCA) have been increasing worldwide. The relatively low responsiveness of CCA to conventional chemotherapy leads to poor overall survival. Recently, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL or Apo2L) has emerged as the most promising anti-cancer therapeutic agent since it is able to selectively induce apoptosis of tumor cells but not normal cells. In this study, we aimed to investigate the therapeutic effect of TRAIL in CCA cell lines (M213, M214 and KKU100) compared with the immortal biliary cell line, MMNK1, either alone or in combination with a subtoxic dose of 5-fluorouracil (5-FU). We found that recombinant human TRAIL (rhTRAIL) was a potential agent which significantly inhibited cell proliferation and mediated caspase activities (caspases 8, 9 and 3/7) and apoptosis of CCA cells. The combined treatment of rhTRAIL and 5-FU effectively enhanced inhibition of CCA cell growth with a smaller effect on MMNK1. Our finding suggests TRAIL to be a novel anti-cancer therapeutic agent and advantage of its combination with a conventional chemotherapeutic drug for effective treatment of CCA.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.788-795
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• 2009

Vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis under various physiological and pathological conditions. We found that the VEGF isoforms VEGF120, VEGF164, and VEGF188 were expressed in the bovine mammary gland and bovine mammary epithelial cells (bMECs). Expression of VEGF in the mammary gland was significantly higher during the lactation period than during the dry period. Although dexamethasone or prolactin alone had little effect on the expression of VEGF, that in dexamethasone-treated cells was significantly induced after additional treatment with prolactin. Furthermore, the VEGF expression induced by the combination of dexamethasone and prolactin was reduced by PD98059 in a dose-dependent manner. This combination also stimulated the phosphorylation of p44/p42 MAP kinase in these cells. These results strongly suggest that the combination of dexamethasone and prolactin stimulates VEGF expression in bMECs via p44/p42 MAP kinase.

• Journal of Biosystems Engineering
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• v.41 no.1
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• pp.34-42
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• 2016

Purpose: This study was performed to improve the drying quality and drying rate of ginseng slices by combining microwave and far-infrared drying techniques. Methods: Based on single-factor experiments and analyses, a quadratic regression orthogonal rotation combination design was adopted to study the effects of the moisture content at the conversion point between the microwave and far-infrared techniques, the ginseng slice thickness and the far-infrared drying temperature on the chip drying time, the surface color difference value, the nutritional composition and the surface shrinkage rate index. Results: Compared to the far-infrared drying alone, the combined microwave and far-infrared drying resulted in an increase in the saponin content of the ginseng slices and reductions in the drying time, surface color difference, and shrinkage rate. Conclusions: We established a mathematical model of the relationships between the surface shrinkage rate index and the experimental factors using the multi-objective nonlinear optimization method to determine the optimal parameter combination, which was confirmed to be the following: microwave and far-infrared moisture contents of 65%, a ginseng slice thickness of 1 mm, and a far-infrared drying temperature of $54^{\circ}C$.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.60 no.5
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• pp.949-954
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• 2011

This paper presents a comparative study on motor characteristics with specific consideration of radial vibration force in interior permanent magnet synchronous motors (IPMSM) according to pole/slot combinations. Three IPMSM models, 16-pole/15-slot design, 16-pole/18-slot design and 16-pole/24-slot design are built, in which 16-pole/15-slot and 16-pole/18-slot designs provide high winding factor and 16-pole/24-slot design is known as a general pole/slot combination. By coupling finite element analysis (FEA) with equivalent circuit method, motor characteristics, back electro-motive force (Back-EMF), inductances, cogging torque, etc. as well as machine output performances are analyzed and compared. The radial vibration force (RVF) distribution in air gap causing stator vibration and noise is interested. It is expected that this study help with appropriate choice of pole/slot combination in IPMSM design.

• Herbal Formula Science
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• v.18 no.1
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• pp.121-132
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• 2010

Inflammatory reaction is characterized by over-production of inflammatory mediators due to an up-regulation of inflammatory pathways, which produce pro-inflammatory mediators, such as interleukin-1beta (IL-$1{\beta}$), IL-6, tumour necrosis factor alpha (TNF-$\alpha$), prostaglantin $E_2$ ($PGE_2$), and nitric oxide (NO) in U937 cells. We investigate the anti-inflammatory effects of water extracts from Lonicerae Flos and Scutellariae Radix in lipopolysaccharide (LPS)-stimulated U937 cells. Each extract suppressed the production of inflammatory mediators (NO, IL-$1{\beta}$, TNF-$\alpha$, and $PGE_2$) and the expression of inducible NO synthase and cyclooxygenase-2 in LPS- stimulated U937 cells in a dose-dependent manner. These suppressive effects were synergistically increased by their combination. Their combination extract also inhibited NF-${\kappa}B$-DNA complex of NF-${\kappa}B$ binding activity and translocation of NF-${\kappa}B$ from cytosol to nucleus. These results suggest that the combination of water-extractable components of Lonicerae Flos and Scutellariae Radix may be useful for therapeutic drugs against inflammatory immune diseases, probably by suppressing the production of inflammatory mediators.

• Journal of Industrial Technology
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• v.24 no.A
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• pp.37-46
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• 2004

A performance analysis for newly being considered electric rail car office has been made in this study. The major purpose is to examine a proposed design in terms of its capacity and the proposed number of travels (82-84) per day. For this study we first examined the overall system configuration with detailed operational processes of cleaning and inspection. We then developed a simulation model using ARENA and designed input data from 12 selected factors and their interaction effects. A simulation run for each treatment combination of $L_{16}(2^{15})$ orthogonal array was run and 20 batch means were obtained. Through careful analyses of the results obtained, we drew a diversity of suggestions including the best factor level combination. Our confirmation experiments at the optimal level combination further validate the possibility of 82 runs and the consistency in the results.

• Herbal Formula Science
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• v.17 no.1
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• pp.99-111
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• 2009

Rheumatoid arthritis is characterized by focal loss of cartilage due to an up-regulation of inflammatory pathways, which produce pro-inflammatory mediators, such as interleukin-1(IL-1), tumour necrosis factor alpha($TNF-\alpha$), prostaglantin, and nitric oxide(NO). We investigated the anti-arthritic effects of water extracts from Pellodendri cortex and Atractylodis rhizoma in vitro and in vivo. Each extract suppressed the production of inflammatory mediators(NO, $IL-1\beta$, $TNF-\alpha$, and prostaglandin $E_2$) and the expression of inducible NO synthase and cyclooxygenase-2 in lipopolysaccharide-stimulated macrophages in a dose-dependent manner. These suppressive effects were synergistically increased by their combination. The same results were also observed in the rat osteoblast sarcoma cell line ROS17/2.8 stimulated with $IL-1\beta$, $IFN-\gamma,$ and $TNF-\alpha$. Moreover, the combination of these water extracts significantly suppressed collagen-induced mouse arthritis. These results suggest that the combination of water-extractable components of Pellodendri cortex and Atractylodis rhizoma may be useful for therapeutic drugs against rheumatoid arthritis, probably by suppressing the production of inflammatory mediators.

• Biomolecules & Therapeutics
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• v.22 no.4
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• pp.355-362
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• 2014

We have developed a fully automated high throughput drug screening (HTDS) system based on the microfluidic cell culture array to perform combinational chemotherapy. This system has 64 individually addressable cell culture chambers where the sequential combinatorial concentrations of two different drugs can be generated by two microfluidic diffusive mixers. Each diffusive mixer has two integrated micropumps connected to the media and the drug reservoirs respectively for generating the desired combination without the need for any extra equipment to perfuse the solution such as syringe pumps. The cell array is periodically exposed to the drug combination with the programmed LabVIEW system during a couple of days without extra handling after seeding the cells into the microfluidic device and also, this device does not require the continuous generation of solutions compared to the previous systems. Therefore, the total amount of drug being consumed per experiment is less than a few hundred micro liters in each reservoir. The utility of this system is demonstrated through investigating the viability of the prostate cancer PC3 cell line with the combinational treatments of curcumin and tumor necrosis factor-alpha related apoptosis inducing ligand (TRAIL). Our results suggest that the system can be used for screening and optimizing drug combination with a small amount of reagent for combinatorial chemotherapy against cancer cells.

• Proceedings of the KSME Conference
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• 2001.06a
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• pp.211-216
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• 2001

The components with the circular cross section have the symmetric combination parts for rotating balance and the crack emanates from the symmetric combination parts. The symmetric cracks from symmetric combination parts make a decrease in the component fatigue life more than single crack. In this study, to estimate the behavior of symmetric cracks, the fatigue test was performed using rotary bending tester on the specimen with a symmetric defects in circular cross section. The material used in this study is Ni-Cr-Mo steel alloy. Under the same stress, the result from the rotary bending fatigue test turned out that the symmetric cracks made a decrease in the fatigue life by 35% more than single crack and the relation between log a and cycle ratio $N/N_f$ obtained linearly.

• Journal of the Korean Society of Clothing and Textiles
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• v.28 no.3_4
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• pp.460-471
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• 2004

The purpose of this study is to investigate the effect of hair-style (straight and wave), hair-length(short, medium, and long), and type of hat design (no-hat, beret, cloche, bowler, and capeline) on impression formation. The experimental materials developed for this study were a set of stimulus and response scales. The 7-point semantic differential response scales were designed. Thirty stimuli color pictures of various combination of hair length, hair style, and type of hat design were manipulated by computer drawing. The subjects were 360 undergraduates living in Seoul, Kyunggi and Kyungnam. The experimental design was composed of 3 factorial designs. The result of the study can be summarized as follows; Impression factor of the stimulus consisted of the 5 different dimensions (attractiveness, gracefulness, activity, concentration of attention, cuteness). Hair style greatly affected gracefulness. On the other hand, hair length made remarkable effects on attractiveness, concentration of attention, and cuteness. In addition, it has been found that the type of hat design significantly influenced on all the factors except attractiveness as independent variables. In regarding the effect of interaction between each variable, the combination of hair style and hair length had significant effects on gracefulness. In the impression formation of cuteness, the combination of hair length and type of hat design were more significant.