• Title/Summary/Keyword: column storage

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Partial Purification of Fig Pectinesterase and Characterization of its in situ Activity (무화과 펙틴에스테라제의 부분 정제 및 in situ 상태에서의 활성 특성)

  • Hou, Won-Nyoung;Kim, Myoung-Hwa;Go, Eun-Kyoung
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1169-1178
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    • 1998
  • This study was performed to purify fig pectinesterase(F-PE) and characterize its in situ activity. Three kinds of F-PE were partially separated by using ammonium sulfate fractionation, Q-Sepharose column, CM-cation exchanger column chromatography, and HPLC. One of those was anionic protein and the others were cationic proteins. All of them had approximate molecular weight of 27,000 and lost rapidly their activity during storage. Therefore alternative crude enzyme was prepared by suspending the freeze dried and milled fig powder in 0.1 M NaCl at pH 7.5. F-PE had the optimum pH of 8.5, the optimum temperature of $50^{\circ}C$ with activation energy of 7,671 cal $mol^{-1}K^{-1}$ and stability up to $55^{\circ}C$ with 10 minutes heating. Optimum activity was obtained in $0.2{\sim}0.4$ M NaCl with optimum solubility at above 0.8 M NaCl.

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Microbial Desulfurization of Coal by Iron-Oxidizing Bacteria Thiobacillus ferrooxidans in packed beds (철산화 박테리아 Thiobacillus ferrooxidans를 이용한 충전탑 반응기에서의 석탄의 생물학적 탈황)

  • 류희욱
    • KSBB Journal
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    • v.14 no.1
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    • pp.124-130
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    • 1999
  • To evaluate the technical of microbial coal desulfurization during the storage in coal dumps, microbial pyrite oxidation in a packed column reactor with Thiobacillus ferrooxidans has been investigated. For microbial desulfurization in a packed reactor system, coal particle size over 1.0 mm with uniform size distribution seems to be most suitable as fas as drainage behavior and accessability of pyrite are concerned. When coal samples of 1∼2 and 2∼4 mm particle size were size were used, about 32∼42% of pyritic sulfur was removed within 70 days. The rate of pyritic sulfur oxidation was in the range of 348∼803 mg S/kg coal ·d, and the sulfur removal rates in packed columns were about 15∼25% of those in suspension cultures. Without any circulation of liquid medium, microbial coal desulfurization could be possible by the inoculation of T. ferrooxidans along on the coal dump. It was concluded that a microbial percolation process is one of possible processes for the desulfurization of high sulfur coal during a long-term storage.

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Effects of Contaning Pigments of Dried Grasshopper on the Lipid Deterioration (벼메뚜기 건제품중의 지질열화에 함유색소성분이 미치는 영향)

  • Lee, Jong-Ho;Kim, Tae-Soo;Choi, Byeong-Dae;Kim, Gyeong-Eup;Lee, Kang-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.4
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    • pp.300-305
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    • 1987
  • Changes in pigments of freeze dried grasshopper in refrigerator were determined to asses the storage stability. In storage at $5^{\circ}C$, chlorophyll contents were drastically decreased but pheopytins were increased. The degradation of chlorophylls get under controlled with vacuum package. The pigments extracted from 98 days stored samples were fractionated by silicic acid column and obtained 3 portions (3a, 3b and 3d). These portions were compared Rf values (0.75, 0.56, 0.50, respectively) and absorption spectra (408, 666nm; 419, 651nm; 433, 655nm, respectively) with that of standard and were identifed pheophytin a and b and 'changed' chlorophyll a. Especially, pheophytins a and b were great strength accelerated to lipid oxidation.

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Asymmetric Index Management Scheme for High-capacity Compressed Databases (대용량 압축 데이터베이스를 위한 비대칭 색인 관리 기법)

  • Byun, Si-Woo;Jang, Seok-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.7
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    • pp.293-300
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    • 2016
  • Traditional databases exploit a record-based model, where the attributes of a record are placed contiguously in a slow hard disk to achieve high performance. On the other hand, for read-intensive data analysis systems, the column-based compressed database has become a proper model because of its superior read performance. Currently, flash memory SSD is largely recognized as the preferred storage media for high-speed analysis systems. This paper introduces a compressed column-storage model and proposes a new index and its data management scheme for a high-capacity data warehouse system. The proposed index management scheme is based on the asymmetric index duplication and achieves superior search performance using the master index and compact index, particularly for large read-mostly databases. In addition, the data management scheme contributes to the read performance and high reliability by compressing the related columns and replicating them in two mirrored SSD. Based on the results of the performance evaluation under the high workload conditions, the data management scheme outperforms the traditional scheme in terms of the search throughput and response time.

Chemical Components of Seed Oil of Sapium japonicum Pax. et Hoffm. (사람주나무 종실유의 화학적 조성)

  • Choi, Myung-Suk;Yang, Jae-Kyung;Gang, Byeng-Kuk
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.3
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    • pp.274-282
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    • 2000
  • Physico-chemical characteristics and chemical composition of seed oil of Sapium japonicum were determined by various analysis methods. Physio-chemical characteristics of the extracted oil from fresh seed were specific gravity (0.928), refraction index (1.477), acid value (2.30), saponification number (190.0), and iodine value (126.0). Neutral lipid (93%) in seed oil was identified as major components, followed by glycolipid (4.9%) and phospholipid (1.3%). In GC analysis, nine fatty acids were presented in the seed oil. Among fatty acids, predominant fatty acids were oleic acid (45.8%) and linoleic acid (35.6%). The contents of fatty acids of seed oil somewhat varied with their storage period. Composition of fatty acids from lipid fractions which were isolated on silica open column was investigated. Major fatty acids in three lipid fractions were linoleic acid and linolenic acid. The fatty acid contents of fresh seed oil derived from each lipid fraction were generally high comapred to storage seed oil. From the above results, seed oil of Sapium Japonicum could be useful in cosmetics, detergents and a few pharmaceuticals.

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Development on the Purification Process of Natamycin from Streptomyces natalensis ATCC27448 (Streptomyces natalensis ATCC27448이 생산하는 natamycin의 정제법 개발)

  • 이창권;장한수;김종태;황용일
    • Journal of Life Science
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    • v.14 no.2
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    • pp.225-228
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    • 2004
  • Natamycin, produced by Streptomyces natalensis ATCC27448, is a polyene macrolide antibiotic, is widely used in the food industry in order to prevent mould contamination. This study carried out to develop an efficient purification process of natamycin from fermentation broth. The stability of natamycin in fermentation broth during storage period was investigated at 4$^{\circ}C$ and room temperature. After the storage of fermentation broth for 14 days at 4$^{\circ}C$, residual activity of natamycin was about 80% but decreased by 27% at room temperature. As solvent to extract natamycin from fermentation broth, methanol was the most efficient. A developed purification procedure includes methanol extraction and Diaion HP-20 column chromatography. Approximately 2.9 g of natamycin was obtained with a final yield of 69.1% and purity of 96.6% from 1.8 l of fermentation broth by this developed purification procedure.

Process Improvement and Evaluation of 0.1 MW-scale Test Bed using Amine Solvent for Post-combustion CO2 Capture (0.1 MW급 연소후 습식아민 CO2 포집 Test Bed 공정개선효과 검증)

  • Park, Jong Min;Cho, Seong Pill;Lim, Ta Young;Lee, Young ill
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.1
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    • pp.103-108
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    • 2016
  • Carbon Capture and Storage technologies are recognized as key solution to meet greenhouse gas emission standards to avoid climate change. Although MEA (monoethanolamine) is an effective amine solvent in $CO_2$ capture process, the application is limited by high energy consumption, i.e., reduction of 10% of efficiency of coal-fired power plants. Therefore the development of new solvent and improvement of $CO_2$ capture process are positively necessary. In this study, improvement of $CO_2$ capture process was investigated and applied to Test Bed for reducing energy consumption. Previously reported technologies were examined and prospective methods were determined by simulation. Among the prospective methods, four applicable methods were selected for applying to 0.1 MW Test Bed, such as change of packing material in absorption column, installing the Intercooling System to absorption column, installing Rich Amine Heater and remodeling of Amines Heat Exchanger. After the improvement construction of 0.1 MW Test Bed, the effects of each suggested method were evaluated by experimental results.

A Study on the Practical Use of the Inn0er Part of a Roof of the New Hanok with a Structural Modification (지붕가구법의 변용을 통한 신한옥 보꾹공간의 활용에 대한 연구)

  • Kim, Hark-Rae
    • Journal of the Korean Institute of Rural Architecture
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    • v.14 no.2
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    • pp.1-10
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    • 2012
  • The purpose of this study is to provide the method of saving the construction cost per unit area of the New Hanok using the inner space of a roof as a floor space with a structural modification of it's roof. The proportion of a roof of the Traditional Hanok accounts for about a half of it's elevation, so it is an essential element of it. But, compare to the whole construction cost of the Traditional Hanok, it costs over a half of expenses to build it. Recently, at the traditional building type, it is found that the inner space of a roof of it is used as a floor space with a structural modification of it's roof. It can be divided into two types, the storage type and the living type. The New Hanok at downtown can accommodate various new lifestyle, so the length of it's Yangtong is longer than that of the Traditional Hanok. When we adjust the proportion of the traditional Hanok to the New Hanok, the height of the column and the roof of the New Hanok becomes also higher than those of the traditional Hanok. So, using the upper part of the column and the roof of the New Hanok, we can make the inner space of a roof of the New Hanok as a floor space -like a bed room, tea room, personal work space and a storage- vertically connect with the 1st floor as the main living area. As a result, it is expected to save the unit construction cost of the New Hanok and has an extensity of space when we build the New Hanok at downtown.

Changes in Total Glucosinolates Levels and Physico-Chemical Properties of Kimchi using Korean Chinese Cabbage of Harvest Time according to Various Storage Conditions (수확기간별 배추를 이용한 김치의 저장조건에 따른 Total Glucosinolates 함량 및 이화학적 변화)

  • Jung, Ji-In;Hong, Eun-Young;Kim, Mee-Kyung;Jung, Ji-Won;Oh, Ji-Young;Kwon, Min-Soo;Lee, Kang-Pyo;Kim, Gun-Hee
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.612-617
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    • 2009
  • Kimchi is a traditional pickled food using Korean Chinese cabbage(Brassica campestris var. pekinensis) and also containing phytochemicals, glucosinolates. This study was carried out to investigate the changes in the total glucosinolates levels of Kimchi using Korean Chinese cabbage of harvest time(June-July, August-September, October-November, December-April, May) according to storage temperature(4, 10, $15^{\circ}C$) and storage duration(0, 1, 3, 5, 7, 10, 14, 21 and 28 day). For determination of glucosinolates, 50g of Baechu kimchi was used for analytical sample preparation provided with an anion exchanges column and measured by UV-visible Spectrophotometer. The highest contents of water occurred at August-September during fermentation. At 15, 10, $4^{\circ}C$, the pH in all of seasonal variation of Baechu kimchi declined, especially from 0 to 3 days at $15^{\circ}C$, from 0 to 7 days at $10^{\circ}C$ and from 0 to 14 days at $4^{\circ}C$. At that storage, the total glucosinolates levels in all of seasonal variation of Kimchi declined in storage temperature. Baechu kimchi at August-September showed the highest total glucosinolates levels. Also total glucosinolates levels decreased as storage period increased. Baechu kimchi fermented at $15^{\circ}C$ for 7days decreased rapidly and reached to the lowest at the 1day(ranged from $10.3{\pm}0.70$ to $23.4{\pm}0.37{\mu}mol/g$ dry weight). At $10^{\circ}C$ for 14days were ranged from $12.9{\pm}0.29$ to $33.7{\pm}1.81{\mu}mol/g$ dry weight before fermentation and decreased rapidly at the 3day(ranged from $9.5{\pm}0.54$ to $20.5{\pm}0.61{\mu}mol/g$ dry weight). Total glucosinolates levels of Baechu kimchi fermented at $4^{\circ}C$ for 28days decreased rapidly during 7day(ranged from $9.9{\pm}0.78$ to $21.1{\pm}0.96{\mu}mol/g$ dry weight) and then slowly decreased. Therefore the total glucosinolates levels decreased during storage time and depended on harvest time and storage conditions of Kimchi.

파쇄 폐타이어가 혼합된 생물학적 반응벽체에 관한 연구 : 폐타이어와 미생물의 MTBE (Methyl tertiary Butyl Ether) 흡착

  • 정수봉;이재영;최상일
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.23-26
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    • 2004
  • Methyl Tertiary-Butyl Ether is one of several fuel oxygenates added to gasoline to improve fuel combustion and reduce tile resulting concentration of hydrocarbon. Thus, MTBE transfer readily to groundwater from gasoline leaking from Underground Storage Tank. Therefor, there are significant risks and costs associated with the water contamination. MTBE is far more water soluble than gasoline hydrocarbon. The purpose of the this study is to test the ability of ground tire with facultative bacteria. Bacillus brevis, to sorb MTBE. The process is consisted both batch and column experiment to determine the sorption capacity. And Biofilm is observed by SEM in the column. Finally, it is clear that ground tire represent an attractive and relatively inexpensive sorption medium for a MTBE. The authors can surmise that to determine the economic cost of ground tire utilization, tile cost to sorb a given mass of contaminant by ground tire will have to be compared to currently accepted sorption media. and Bacillus brevis strain was eliminated on MTBE, too. The biobarrier that ground tire with bacteria, has potential for use in the remediation of MTBE-contaminated environments.

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