• Title/Summary/Keyword: colormetric determination

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Colorimetric Determination of Acidic Polysaccharide from Panax ginseng, its Extraction Condition and Stability (인삼 산성다당체의 비색정량 방법과 그 추출조건 및 안정성)

  • 도재호;이형옥
    • Journal of Ginseng Research
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    • v.17 no.2
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    • pp.139-144
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    • 1993
  • The method for colorimetric determination of acidic polysaccharide from Panax ginseng was investigated. It is possible to apply the method of carbazole-sulfuric acid to determination of pectin, and also to measure the amount of pectin in the mixture of various high molemlu compounds such as starch. cellulose and gum, etc. When the method of carbazole-sulfuric acid was applied to determine the amount of acidic polysaccharide, optical density at 525 nm increased linearly with an increase in the concentration of pure acidic polysaccharide. Effective extraction temperature with water for the determination of the amount of ginseng acidic polysaccharide (GAP) was $80{\circ}C$. In order to separate or concentrate GAP it was appropriate to precipitate the extract only once with 80% ethyl alcohol. GAP was very stable at $100{\circ}C$ for 4 hrs in aqueous solution and between pH values of 5.0~ 12.0.

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Application of a Diode Laser Colormetric Spectrometer to Determination of Cetylpyridinium Chloride (다이오드 레이저 비색 분광기를 이용한 Cetylpyridinium Chloride거 농도분석)

  • Keun-Woo Park;Se-Yun Kim;Chul-Min Shin;Jeong-Woon Seo;Hye-Jin Hyun;Hae-Seon Nam;Sung-Ho Kim
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.4 no.3
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    • pp.257-259
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    • 2003
  • 본 논문은 다이오드 레이저와 광 다이오드를 사용한 이중 빗살형 다이오드 레이저 비색 분광기를 개발하여 항균 성분의 양이온 계면 활성제로 널리 사용되고 있는 cetylpyridinium chloride(CPC)의 농도를 측정하였다 분광기의 안정도는 광원의 세기, 감도, 재현성을 측정한 예비 실험을 통하여 검증이 되었다 또한 상용화된 UV/VIS분광기와의 비교 결과를 나타내었다. 다이오드 비색 분광기는 3×10/sup -5/M에서 1.1×10/sup -4/M의 CPC 농도 범위에서 0.9635의 상관계수를 나타내었다. 이러한 결과는 CPC의 농도 분석을 위한 간편한 다이오드 레이저 비색 분광기 개발의 가능성을 나타내었다.

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