• The Plant Pathology Journal
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• 제16권4호
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• pp.200-205
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• 2000

Biological control of blue stain fungi, such as Ophiostoma and Leptographium spp., that reduce the quality of logs and cause economic losses in wood product industry, was carried out in laboratory and field trials by a colorless strain of Ophiostoma quercus, BSFcs-1. Inoculation of pine wood chips with the colorless strain 1 wk before inoculating wild-type strain demonstrated that BSFcs-1 colonized wood chips and excluded blue stain fungi from being established. Efficacy of BSFcs-1 was compared with colorless strain of O. piliferum, which is commercially available under the trade name of Cartapip. Inoculation of pine wood logs with the colorless strain 1 wk before inoculating wild-type strain of blue stain in isolated wood chips, while O. quercus and O. floccosum colonized 0% and 17%, respectively. Simultaneous inoculation of logs with the colorless and wild-type strains resulted in decreased colonization (28%) by BSFcs-1, but increased colonization by O. quercus (185) and O. floccosum (29%). On the other hand, BSFcs-1 and wild-type strain alone colonized 75% and 71%, respectively. Treatment of the surface of log ends with mycelial suspension of BSFcs-1 after cutting also showed good control of blue stain fungi in a pine forest stands.

• 반도체디스플레이기술학회지
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• 제20권4호
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• pp.49-54
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• 2021

The display industry is transitioning from traditional rigid products such as flat panel displays to flexible or wearable ones designed to be folded or rolled. Accordingly, colorless and optically transparent polyimide (CPI) films are one of the prime candidates to substitute traditional cover glass as a passivation layer to accommodate product flexibility. However, CPI films subjected to repetitive pure bending loads inevitably entail an accumulation of residual strain that can eventually cause wrinkles or delamination in the underlying component after a certain number of static and cyclic loading. The purpose of this study is to establish an experimental method to systematically evaluate the bending residual strain of CPI films. Films were monotonically and cyclically wrapped on mandrels of various diameters to ensure a constant strain in each. After unwrapping the wound CPI film, the residual radius of curvature remaining on the film was measured and converted into residual strain. The critical radius of curvature at which residual strain does not remain was about 5 mm, and the residual strain decreased in proportion to the log time. It is expected that flexible displays can be reliably designed using the data between the applied bending strain and the residual strain.

• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1691-1699
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• 2018

A metabolically-engineered Deinococcus radiodurans R1 strain capable of producing phytoene, a colorless $C_{40}$ carotenoid and a promising antioxidant, has been developed. To make this base strain, first, the crtI gene encoding phytoene desaturase was deleted to block the conversion of phytoene to other carotenoids such as lycopene and ${\gamma}$-carotene. This engineered strain produced $0.413{\pm}0.023mg/l$ of phytoene from 10 g/l of fructose. Further enhanced production of phytoene up to $4.46{\pm}0.19mg/l$ was achieved by overexpressing the crtB gene encoding phytoene synthase and the dxs genes encoding 1-deoxy-$\text\tiny{D}$-xylulose-5-phosphate synthase gene, and by deleting the crtD gene. High cell-density culture of our final engineered strain allowed production of $10.3{\pm}0.85mg/l$ of phytoene with the yield and productivity of $1.04{\pm}0.05mg/g$ and $0.143{\pm}0.012mg/l/h$, respectively, from 10 g/l of fructose. Furthermore, the antioxidant potential of phytoene produced by the final engineered strain was confirmed by in vitro DPPH radical-scavenging assay.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.182-187
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• 2004

Strain NR $15-1^T$ isolated from traditional Korean Nuruk is described as a new species and named as Aspergillus coreanus NR $15-1^T$ sp. novo Strain NR $15-1^T$ grew rapidly to form yellow-green colonies whose surfaces were velvety on Czapek solution agar. Conidial heads were yellow to light and elliptical, whereas the conidiophore was colorless and typically long. In addition, vesicles were from flask-shaped to globose, and sterigmata are uniseriate. Conidia were spherical and deep yellow-green, and their surfaces were lightly roughened. The G＋C content of strain NR $15-1^T$ was 51 mol% and strain NR $15-1^T$contained a dihydrogenated ubiquinone with Q9 (94.9%) as a major quinone. The nucleotide sequences of strain NR $15-1^T$ in the two Internal Transcribed Spacers (ITS 1 and 2) and 5.8S rDNA showed highest similarity when compared with that of A. tubingensis and A. phoenicis NRRL $365^T$. However, based on morphological and chemotaxonomic characteristics, this strain was different from A. tubingensis and A. phoenicis NRRL $365^T$. On the basis of the data presented, it is proposed that strain NR $15-1^T$ should be placed in the genus Aspergillus as a new species, Aspergillus coreanus sp. novo Therefore, the type strain of the new species is strain NR $15-1^T$ (=KCTC 18075P^T,=KCCM 80006^T$.

• 한국공간구조학회논문집
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• 제10권1호
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• pp.51-58
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• 2010

ETFE 필름 막재는 Ethylen Tetra Fluoro Etylene의 약자로, 무색의 투명한 막재료이다. ETFE 필름 막재는 내화학성이 있고 매우 가벼운 장점이 있는 것으로 알려져 있다. ETFE 필름 막재는 $50{\mu}m$에서 $300{\mu}m$의 두께가 주로 사용되며, 인장강도는 40MPa에서 60MPa 300%에서 400%정도이다. 본 연구에서는 최근 해외에서 초대형 박구조물의 박재료로 시용되는 ETFE 필름 막재에 대한 기초적 역학적 특성을 조사하기 위하여 인장시험을 수행하였다. 인장시험으로부터 인장강도, 인장연신율, 영계수(Young's Modulus) 등 건축 설계 시 필요한 기초적 자료를 제공하고자 한다.

• 한국공간구조학회논문집
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• 제9권2호
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• pp.77-82
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• 2009

ETFE 막재는 Ethylene Tetra Fluoro Ethylene의 약자로 색깔이 없고, 투명한 필름 막이다. ETFE 필름의 장점은 내화학성이 있고, 잘 접히지 않으며, 매우 가볍운 재료라는 것이다. 필름의 두께는 50마이크로 미터에서 300 마이크로 미터 두께가 주로 사용되고, 직포가 없어며 햇빛 투과율이 우수하고 재료의 강도는 다른 막재에 비해서 낮다. ETFE 막재의 인장강도는 40MPa에서 60MPa 정도이고, 인장 변형도는 약 200%에서 400% 정도이다. 본 논문에서는 ETFE 필름 막재의 역학적특성 시험을 수행하였다. 인장 시험으로 부터 인장 변형도, 인장 강도, 응력 변형도 곡선을 구하였고, ETFE 막재의 항복 강도를 결정하여 탄성계수를 구하였다. 그리고 온도하중에 의한 응력-변형도 특성과 반복하중에 대한 필름의 역학적 특성을 분석하였다.

• Mycobiology
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• 제51권3호
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• pp.115-121
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• 2023

The fungal strain KNUF-22-18B, belonging to Cucurbitariaceae, was discovered from a stink bug (Hygia lativentris) during the investigation of insect microbiota in Chungnam Province, South Korea. The colonies of the strain KNUF-22-18B were wooly floccose, white to brown in the center on oatmeal agar (OA), and the colonies were buff, margin even, and colorless, reverse white to yellowish toward the center on malt extract agar (MEA). The strain KNUF-22-18B produced pycnidia after 60 days of culturing on potato dextrose agar, but pycnidia were not observed on OA. On the contrary, N. keratinophila CBS 121759^T abundantly formed superficial pycnidia on OA and MEA after a few days. The strain KNUF-22-18B produced chlamydospores subglobose to globose, mainly in the chain, with a small diameter of 4.4-8.8 ㎛. At the same time, N. keratinophila CBS 121759^T displayed a globose terminal with a diameter of 8-10 ㎛. A multilocus phylogeny using the internal transcribed spacer regions, 28S rDNA large subunit, b-tubulin, and RNA polymerase II large subunit genes further validated the uniqueness of the strain. The detailed description and illustration of the proposed species as Neocucurbitaria chlamydospora sp. nov. from Korea was strongly supported by molecular phylogeny.

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.483-489
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• 1997

Sulforhodamine B (SRB) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, RNA dot blot and Northern hybridization analysis were performed to screen microorganisms for the production of anticancer agent. Among microorganisms tested, strain YP-1 was selected for its cytotoxicity and ability to reduce the level of c-myc RNA. Strain YP-1 was identified as Streptomyces endus. The anticancer material produced by Streptomyces endus YP-1 was sequentially purified by solvent extraction, silica gel column chromatograpby, preparative TLC and preparative HPLC. The cancer material identified as azalomycin B by the instrumental analyses such as $^{1}$H-NMR, $^{13}$C-NMR, Mass, IR and UV absorption. It was colorless amorphous powder and its molecular weight was 1025.278. Azalomycin B, produced by Streptomyces endus YP-1, showed anticancer activity against several human cancer cell lines and reduction of c-Myc protein level in Colo320 DM cells which was determined by Western blot analysis.

• Journal of Microbiology and Biotechnology
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• 제21권11호
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• pp.1184-1192
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• 2011

A bacterial isolate (strain JS-2) characterized as Bacillus sp. was challenged with high concentrations of toxic selenite ions. The microbe was found to transform the toxic, soluble, colorless selenite (${SeO_3}^{2-}$) oxyions to nontoxic, insoluble, red elemental selenium ($Se^0$). This process of biotransformation was accompanied by cytoplasmic and surface accumulation of electron dense selenium ($Se^0$) granules, as revealed in electron micrographs. The cells grown in the presence of selenite oxyions secreted large quantities of extracellular polymeric substances (EPS). There were quantitative and qualitative differences in the cell wall fatty acids of the culture grown in the presence of selenite ions. The relative percentage of total saturated fatty acid and cyclic fatty acid increased significantly, whereas the amount of total unsaturated fatty acids decreased when the cells were exposed to selenite stress. All these physiological adaptive responses evidently indicate a potentially important role of cell wall fatty acids and extracellular polymeric substances in determining bacterial adaptation towards selenite-induced toxicity, which thereby explains the remarkable competitiveness and ability of this microbe to survive the environmental stress.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1065-1070
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• 2017

This study aimed to examine the anti-candidal efficacy of a novel ketone derivative isolated from Diaporthe sp. ED2, an endophytic fungus residing in medicinal herb Orthosiphon stamieus Benth. The ethyl acetate extract of the fungal culture was separated by open column and reverse phase high-performance liquid chromatography (HPLC). The eluent at retention time 5.64 min in the HPLC system was the only compound that exhibited anti-candidal activity on Kirby-Bauer assay. The structure of the compound was also elucidated by nuclear magnetic resonance and spectroscopy techniques. The purified anti-candidal compound was obtained as a colorless solid and characterized as 3-hydroxy-5-methoxyhex-5-ene-2,4-dione. On broth microdilution assay, the compound also exhibited fungicidal activity on a clinical strain of Candida albicans at a minimal inhibitory concentration of $3.1{\mu}g/ml$. The killing kinetic analysis also revealed that the compound was fungicidal against C. albicans in a concentration- and time-dependent manner. The compound was heat-stable up to $70^{\circ}C$, but its anti-candidal activity was affected at pH 2.